Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,980,160 G→A 29.2% noncoding (641/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,980,1600GA29.2% ‑2.7 / 23.5 36noncoding (641/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (11/14);  new base A (2/8);  total (13/23)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC  >  NZ_CP034943/2980046‑2980296
                                                                                                                  |                                                                                                                                        
acgtCACCCGGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCt                                                                                                                 >  1:3949463/4‑140 (MQ=1)
         tggccttGGGTAGATCACCTGGGTTCGGGTCTACGACCACGTACTCAGGCGACCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCGTCTGCTTAACCTTGCACAGGATCGCAACTCGCCGGTTCATTCTACAAAAGGc                                                                                                        <  2:5624267/134‑1 (MQ=1)
          ggccATGGGTAGATCACCTGGTTTCGGCTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTCCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCa                                                                                                       <  2:3222558/137‑1 (MQ=0)
                ggctAGATCACCTGGTTTCGGGTCTACGAACACGTACTCATGCGCCCTATTCAGAATCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGAGCGTCACTCGCCGGTTCATTCTACAAATAGCACGCCAt                                                                                                 >  2:7153116/4‑140 (MQ=1)
                 catacaTCACCTGGTTTCGGGTATAATAAAAATAACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATc                                                                                                >  1:893596/6‑140 (MQ=1)
                     gaacACCTGGTTTCGGGTCTACTCCCACGTACTCATGCTCACTATTCAGTCTCGCTGACGCTGCCGCTCCGCATCTTGAGCTTAACCTTGCCCAGGAGCGTAACTCGCCCGTTCAGTCTACAAAAGGCACCCAAACAccc                                                                                            >  2:5403698/4‑140 (MQ=0)
                          ccgggTTTCGGGTCTACGACCACGTACTCATGCGCCCGATTCAGACTCGCTTTCGCTGCGGCTCCGCATCGTCTCCTTAACCTTGCACAGCAGCGTAACTCGCCGGGTCATTCTACAAAAGGCACGCCATCACCCGTTaa                                                                                       <  2:6182217/137‑1 (MQ=1)
                          ccgggTGTCGGGTCTACGACCACGTACTCATGCGCCCTCTTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTGGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTaa                                                                                       <  1:5779532/137‑1 (MQ=1)
                             gggttCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCAGTCTACAAAAGGCACGACATAACCCGTTAACgg                                                                                    >  2:5034115/4‑140 (MQ=0)
                                ttggggTCTACGACCACGTACTGATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTGCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGct                                                                                 <  1:3017318/137‑1 (MQ=0)
                                     gtatACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGac                                                                            <  1:1743408/137‑1 (MQ=1)
                                                cgaaCTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGAGCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGc                                                                 >  2:1673758/4‑140 (MQ=1)
                                                  taatCATGCGCCCTATTCAGACTCGCTTTCGCTGCGTCTCCGCATCTTCTGCTTAACCTTGCAGGGGATCGTAACTCGCCGTTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGcac                                                               <  2:1154587/137‑1 (MQ=0)
                                                   acacATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGcaca                                                              <  2:4945112/137‑1 (MQ=1)
                                                   acacATGCGCCCTAGTCAGACTCGCTGTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCCTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGGTAACGGCCTCTGACTACTTGTAGGcaca                                                              <  1:6332604/137‑1 (MQ=1)
                                                     tcttGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACg                                                            >  2:1694126/4‑140 (MQ=1)
                                                       attcgcCCTATTCAGACTCGCTGTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGGTCGTCACTCGCGGGTGCCTTCTACAAACGGCACGGCATCACCCGTTACCGGGCTCTGACTACTTGTAGGCACACGGt                                                          >  2:4359730/4‑140 (MQ=1)
                                                       attcgaccTATACAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGAAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAGTACTTCTACGCACACGGt                                                          >  2:4339678/7‑140 (MQ=1)
                                                             ccgaTTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCGTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCACTGACTACTTGTAGGCACACGGTTTCAgg                                                    <  2:7593800/137‑1 (MQ=0)
                                                               tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt                                                  <  2:6813688/137‑1 (MQ=1)
                                                               tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt                                                  <  1:1492842/137‑1 (MQ=1)
                                                               tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt                                                  <  1:357430/137‑1 (MQ=1)
                                                               tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt                                                  <  2:5099727/137‑1 (MQ=1)
                                                               tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGGTTCAGGAt                                                  >  2:5524035/4‑140 (MQ=0)
                                                               taatCAGACTCTCTTTCGATCCTGCTCCGCATCTTCTGCTTAACCTTGCAAGTGATAGTAACTCTACGGTTCATTCTACAAAAGGCACGCAAACACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt                                                  >  2:4211218/4‑140 (MQ=1)
                                                                        acgctcgcGCTGCGGCACCGCCCCGTCGGCTGACCCGTGCACCGGATCGTAACTCGCCGCTTCCTTCTACAAACGGCACGCCATCACCCCTCCACGGCCTCTGACTACTGGGAGGCACACGGTTTCAGGATCTCTTTCAc                                         <  2:1665155/133‑1 (MQ=0)
                                                                          gcgttCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc                                       <  2:4842035/137‑1 (MQ=1)
                                                                           ctgtCGCTGCGGCTCCGCCTCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTcc                                      >  2:6043654/4‑140 (MQ=1)
                                                                                gcggCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTc                                 <  1:3557742/137‑1 (MQ=1)
                                                                                    cgtcTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCTGTTCATTCTACAAAAGGCACGCCATGACCCGTTAACGGGCTCTGACTACTTGTAGGCAGACGGTTTCAGGATCTCTTTCACTCCCCTTCCggg                             <  2:4120584/137‑1 (MQ=1)
                                                                                    cgccTCCGCATCTTCTGCTTAACCTTGCACGGCATCGTAACTCGCCGGTTCATTCAACAAAAGGCACGCCCTCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGGGTCAGGATGTCTTTCACTCCCCTTCCggg                             >  2:3763257/4‑140 (MQ=1)
                                                                                                gtcagCTTACCCTTGCACGGGATCGGAACTCGCCGGTTCCTTCTACACAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCGTCAGGGGTGCTTTTCAcc                 <  1:6091356/136‑1 (MQ=1)
                                                                                                 tcggCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCt                <  2:602999/137‑1 (MQ=1)
                                                                                                 tcggCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCGTCCGGGGTGCTTTTCACCt                <  1:640414/137‑1 (MQ=1)
                                                                                                         acgttGCACGGGATCGTAACTCGCCGGTTAATTCTACAAAAGGCACGCCATCACCCGGTACCGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa        <  2:4460349/137‑1 (MQ=0)
                                                                                                          ccgtGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc       <  1:4996262/137‑1 (MQ=1)
                                                                                                               caagggATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAc  <  1:354881/137‑1 (MQ=1)
                                                                                                                  |                                                                                                                                        
ACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC  >  NZ_CP034943/2980046‑2980296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑