Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,991,227 T→G 24.4% intergenic (+28/‑179) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2270TG24.4% 348.6 / 70.5 228intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base T (56/108);  new base G (60/2);  total (116/112)
Fisher's exact test for biased strand distribution p-value = 1.97e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363
                                                                                                                                        |                                                                                                                                            
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAACTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttt                                                                                                                                           >  2:2564823/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttt                                                                                                                                           >  2:7536299/1‑140 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTGTTtgtg                                                                                                                                        >  1:4069787/1‑140 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgtttttt                                                                                                                                        >  1:7403962/1‑139 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttttgt                                                                                                                                        >  1:5269508/1‑138 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTg                                                                                                                                        >  1:3198917/1‑140 (MQ=255)
     cgCACCTGGAACCCTCTAACGCTTACTACGGGGGGCTGAAACGGCGTGCTGAACCGGGGCCGCGCGGGGAAATTTTTAAATTTACCCCTATGGAAACAACGTAACAAAAACACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                      <  1:237577/139‑1 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                      >  1:6159986/1‑140 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTGTGtt                                                                                                                                      >  2:4984815/1‑140 (MQ=255)
       cAACTGGAAAGCTCTCAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTtgtttgttgt                                                                                                                                    >  2:2769088/1‑138 (MQ=255)
       cAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGttttttttttgt                                                                                                                                    >  2:6198320/1‑138 (MQ=255)
       cAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGttttttttttgt                                                                                                                                    >  1:3259268/1‑138 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGttttttttttta                                                                                                                         >  1:3414563/1‑134 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTTTTGTTTTTTTTGtta                                                                                                                         >  1:4686191/1‑138 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTTGttttttttttta                                                                                                                         >  1:6562788/1‑134 (MQ=255)
                   tctactgCTTACTATGATTGGCTTAAAGGGCTTGCTGAATCGGTCCCCTGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                        <  1:4501695/134‑1 (MQ=255)
                   tctAAAGGTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                        <  2:1471651/140‑1 (MQ=255)
                   tctAAAGCTTACTTTGATGGGCTGAAACGGCTTGCTGAATCGGTGCAGCGCGGTTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                        <  1:3329914/140‑1 (MQ=255)
                   tctAAAGCTTACTATGTTTGGCTGAAACGGCTTGATTAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAAAAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                        <  2:5983092/140‑1 (MQ=255)
                   aatAAAGCTTACTATGTTGGGCTTAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAATTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                        <  1:1664238/138‑1 (MQ=255)
                    ctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTaaat                                                                                                                       >  2:1210309/1‑138 (MQ=255)
                       aaGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTttattaat                                                                                                                    >  1:3511160/1‑138 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTTTTTTTTTTGTAATTAATAaaaa                                                                                                             >  2:2893823/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAAATAATAaaaa                                                                                                             >  2:1664238/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTTTTTTTTTTTTAATTAATAaaaa                                                                                                             >  1:2026200/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATTAATAaaaa                                                                                                             >  1:433287/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTAATACaaa                                                                                                             >  1:495164/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTTTGTAATTAATACaaa                                                                                                             >  1:853005/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACAGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTCGTTTTTTTTTAATTACGAaaaa                                                                                                             >  2:892473/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATAACGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTTATTAATAaaaa                                                                                                             >  2:6724937/1‑140 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGTTTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTACAAAt                                                                                                            >  2:4109985/1‑140 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTAATAaaaaa                                                                                                            >  2:2734923/1‑139 (MQ=255)
                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTAATTTATAAAAATTTTaaa                                                                                                      >  2:4049327/1‑140 (MQ=255)
                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTTTTTTTTTTTTAAATTCTACAAAATTGaaaa                                                                                                     >  1:7542732/1‑140 (MQ=255)
                                        cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTAATACAAAATTGaaaaaaaa                                                                                                 >  2:781764/1‑137 (MQ=255)
                                          ctaaCGGGTTGCTTATTGGGTGCAGCGCGTGGATATTTTTATATTTATACCGATGGAAACAACGTAAAACAAACACGCCGGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATACAAATTTGAAAAATACgg                                                                                              <  2:1264411/138‑1 (MQ=255)
                                           aaccGGCTTGCGGCCCCGGTGCAGCTCGGGCAAATTTTAAAATTTACACCGATTGAACCACCGTCACACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAACAATACggg                                                                                             <  1:1287665/137‑1 (MQ=255)
                                           aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGCTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTGTGTTGTTTTTTATGTAATTTCTACAAATTAGAAAAATACggg                                                                                             >  2:3178501/1‑140 (MQ=255)
                                           aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACTAAAAATTTGAAAAATACtgg                                                                                             >  2:4685306/1‑140 (MQ=255)
                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTTTTATTTATACAAATTTTAAAAATAACGGaaaaaa                                                                                       >  1:1496454/1‑139 (MQ=255)
                                                 cTTGCTGAATCGGTGCAGAGAGGGTAAATTTATAAATTTATACCGATTGAAACAAAGTAAAACAAAAACCCCTGGGAAACCGGGAGTTTTTTTGTTATTTTTTATCTAAGTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                       <  2:4393671/140‑1 (MQ=255)
                                                         aTCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                               <  2:4795135/140‑1 (MQ=255)
                                                         aTCGGAGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                               <  2:3309962/140‑1 (MQ=255)
                                                              tGCAGATCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTACTACAAATTTGAAAAATAAGGGAAAAAAATGCCCATTACtt                                                                          >  2:4828474/1‑140 (MQ=255)
                                                              tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTATTAATACAAATTTGTAAAATTGGGGAAAAACATCCCAttttgtg                                                                          >  2:7134527/1‑136 (MQ=255)
                                                               gCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTAATACAAATTTGAAAAATACGGGAAAAACATACCAattcattg                                                                         >  2:6309944/1‑135 (MQ=255)
                                                               gCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTATGTTATTAATAAAAATTTGAAAAATAAGTGTAAAACATACCATTTAAttg                                                                         >  2:4492089/1‑139 (MQ=255)
                                                                 atcgCGGGTAAATTTTTAAATTAATACCTATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCATTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                       <  1:774149/137‑1 (MQ=255)
                                                                   agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATAAAAATTTTAAAAATAAGGGAAAAAAAACCCAGTTACttgccaa                                                                     >  2:7247287/1‑135 (MQ=255)
                                                                       gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACTACAAATTTTAAAAATACGGGAAAAAAATACCATTTACTTCCAAAatat                                                                 >  2:3848620/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAACGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAtt                                                                <  2:3344718/140‑1 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTAAAAAAAAATTTAATAAAACGGGAAAAAAATCCCATTTACATACAAAATAtt                                                                >  2:1845112/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACTAAAAATTTGAAAAATACGGGCAAAAAATCCCATTTAATTCCAAAATAtt                                                                >  2:5143358/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTTATACAAATTTTAAAAATAAGTGAAAAACATACCATTTAATTACAAAATAtt                                                                >  2:5994546/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATTTGTACAAAATTTTAAAATACGGGGAAAAAAACCCAATTTCTTACAAAATAtt                                                                >  1:1511140/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTTTTTATTCAAATTTTTAAAATAGGGGATAAAACTGCCATTTTATTCCAACATAtt                                                                >  1:5610535/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTACTACAAATATGAAAAATAAGGGGAAAAAATCCCAATTAATTACaaaaagtt                                                                >  2:5237485/1‑136 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGACTGGGAAACCGGTCGTTTTTTTGTTCTTTTTTATTTAATTAGTACAAATTTTAAAAATTCTGGAAAAAACTCCCAATTAGAAGCAAAATAtt                                                                >  2:5392168/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATCGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTATTTAATTAATTAAAATATGAAAAATAAGGTGAAAAAATACAATTTACTTGCAAAATAtt                                                                >  2:1056008/1‑140 (MQ=255)
                                                                         taaaTTTTTAAATTTATACCGTTTGAAAAAACGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                               <  2:2537391/139‑1 (MQ=255)
                                                                         gAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACATAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                               <  2:3673433/140‑1 (MQ=255)
                                                                         gAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                               <  2:1602186/140‑1 (MQ=255)
                                                                         gAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                               <  2:5903929/140‑1 (MQ=255)
                                                                              ttttAAATTTATACCTATTCAAACAAATTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                          <  1:384569/140‑1 (MQ=255)
                                                                                             gATTCAAACAACCTAAAACAACCACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                           <  1:6342448/140‑1 (MQ=255)
                                                                                              aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAATTTTAAAAAAAAGGGAAAAACATACCAATTAAATAACAAATATTTGTTGTTAAATTTATGTAGTTa                                         >  2:6410737/1‑140 (MQ=255)
                                                                                                  aaaCAACGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:6280086/140‑1 (MQ=255)
                                                                                                  aaaCAAAGTAACCCAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:4011261/140‑1 (MQ=255)
                                                                                                  aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:4874442/140‑1 (MQ=255)
                                                                                                        aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTGTTTTTTTTTTATTTATTAACAATTTTATAAATAATGGGCAAAAAAACCAATTTCTGACCAAATATTTTTTTGTAATATTTTTTGTGTTAAATTTggtt                               >  1:2672053/1‑137 (MQ=255)
                                                                                                        aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTGTTTTTTATTTAATTAATACAAAATTTAAAAAAAACGGGAAAAAAAACCCATTAATTACAAAATATTTTATGGTTAATTTTTTTAGGTAAAAtttttgt                               >  2:2664309/1‑136 (MQ=255)
                                                                                                        aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTAATGAAAATTTTTAAAAATAAGGTAAAAAAAACCAATTATTTTGAAAAATATTTTTTGTTAATTTTTTTTTGGTGAAAttttgt                                >  1:2260923/1‑137 (MQ=255)
                                                                                                        aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATTAATACAAAATTGAAAAATAACGGAAAAAAAAACCAAATAATTACAAAAAATTTTATGGTAAATTTATTTAGGTAAAATTTTTAt                               >  2:2202608/1‑140 (MQ=255)
                                                                                                         ttAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                              <  2:2026200/139‑1 (MQ=255)
                                                                                                         ctAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                              <  2:433287/139‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1089613/140‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:5764329/140‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2856049/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:3844712/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:4755052/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2734923/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2962608/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:519228/140‑1 (MQ=255)
                                                                                                             aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTACAAAATTTAAAAATAACGGAAAAACATCCCATTTTCGTCCAAAATATTTGATTGTAATTTTATTGAGGTAAAAATTTTAttttgg                          >  2:2418043/1‑137 (MQ=255)
                                                                                                             aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTACTACAAATTTTTAAAATAAGGGAAAAAAAAACCCGTTACTTCCAAAATATTTGTTGGTACTTTTATGTAGGTGAAATTTGTAttttgg                          >  2:837745/1‑137 (MQ=255)
                                                                                                                 acaaCGCCTGGGACACCGGGCGTTTTTTTGTTCTTTTTTATCTAATTACGACAAATATGAAAACTACGGGACAAACATACCATTTACGTCCAAAATAGTTGATGGTAATTTTATGTAGGGAAAATTTGTATTTGGCCGtt                      <  2:1434365/138‑1 (MQ=255)
                                                                                                                 aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTACTTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                      <  1:4128164/137‑1 (MQ=255)
                                                                                                                 aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                      <  1:6725202/137‑1 (MQ=255)
                                                                                                                 aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                      <  2:736228/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:6588940/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:1347962/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:7086922/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:2140565/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:2571864/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTATTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:7247287/140‑1 (MQ=255)
                                                                                                                           ggAAACCGGGAGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:5150803/140‑1 (MQ=255)
                                                                                                                              aaCCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaa         <  1:3797355/140‑1 (MQ=255)
                                                                                                                                ccGGGCGTGTTTTTTGTGTTTTTTATTTAATTACTACAAATTTGATAAATAGGGGCAAAACATCCCAAGTACTTCCAAAATGAGTGATGGTAAGTGTATTTAGGTGAAAATTTTTGTTTGGCGGTCAGCGGAGGtttgaa       >  2:7064237/1‑135 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAACATATTTGATGGTAATTTTATGTAGGGAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAACAGCggg  <  2:1998573/140‑1 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1611585/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                            
ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: