Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,184,761 T→G 5.5% R122R (AGG→CGG)  EO946_RS16485 ← tautomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,184,7610TG 5.5% 1231.3 / 11.7 495R122R (AGG→CGG) EO946_RS16485tautomerase family protein
Reads supporting (aligned to +/- strand):  ref base T (230/215);  new base G (3/47);  total (233/262)
Fisher's exact test for biased strand distribution p-value = 5.90e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 515 total aligned reads displayed.

AACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAC  >  NZ_CP034943/3184622‑3184900
                                                                                                                                           |                                                                                                                                           
aaCCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCt                                                                                                                                             <  1:3010740/140‑1 (MQ=255)
      tcaaactatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCAtt                                                                                                                                       >  2:7582760/1‑140 (MQ=255)
      tcaaactatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCAtt                                                                                                                                       >  2:5896087/1‑140 (MQ=255)
           ctatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  <  1:1018726/140‑1 (MQ=255)
           ctatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  <  2:1852003/140‑1 (MQ=255)
           ctatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  <  1:986033/140‑1 (MQ=255)
           ctatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  <  2:4324135/140‑1 (MQ=255)
           ctatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  <  2:5679927/140‑1 (MQ=255)
             atcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGaaa                                                                                                                                >  1:7071433/1‑140 (MQ=255)
              tcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTTAATCACCGTCCCATTAATAGACCACCCCGCGGGTCTCCATCCGCGGCGTTTTATTATTCAATCATCTGGGCCCTTCCAGGTCCGACAg                                                                                                                               >  2:5172765/1‑140 (MQ=255)
              tcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  <  1:6665100/137‑1 (MQ=255)
                aaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAc                                                                                                                             <  2:7466100/140‑1 (MQ=255)
                     aGAAAACGGCAGAACAAGTAAAAGAGAAAGCGGCACACGCCGTTGAATCACCTACCCATTAATAGACCACCCCGCGGGGAGCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCAATTCAGAAACACCAATc                                                                                                                        >  2:4803185/1‑140 (MQ=255)
                       aaaaCGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAACACCAATCCt                                                                                                                      <  2:2788559/140‑1 (MQ=255)
                        aaaCGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCTGTTATCAATCCGCGGTGTTTTATTATTCATTCATTTGGTTACTTACATTTCCGAAAGACCAATCCTc                                                                                                                     <  2:3056636/140‑1 (MQ=255)
                         aaCGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCa                                                                                                                    <  1:6238294/140‑1 (MQ=255)
                           cGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATc                                                                                                                  <  1:2717684/140‑1 (MQ=255)
                              gagaACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTg                                                                                                               >  1:3675239/1‑140 (MQ=255)
                              gagaACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTg                                                                                                               >  1:6996761/1‑140 (MQ=255)
                              gagaACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTAGTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTg                                                                                                               >  2:5372483/1‑140 (MQ=255)
                                      gTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTAGTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCgg                                                                                                       >  1:3976525/1‑140 (MQ=255)
                                      gTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCgg                                                                                                       >  1:5724493/1‑140 (MQ=255)
                                      gTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCgg                                                                                                       >  1:4968659/1‑140 (MQ=255)
                                           agagAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                                                                  <  2:4025737/140‑1 (MQ=255)
                                              gaAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATg                                                                                               <  1:6308306/140‑1 (MQ=255)
                                               aaaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGa                                                                                              >  1:7380517/1‑140 (MQ=255)
                                               aaaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGa                                                                                              >  1:1899041/1‑140 (MQ=255)
                                                aaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCCGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGAt                                                                                             >  2:5947981/1‑140 (MQ=255)
                                                 aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTc                                                                                                                                           >  1:4044620/1‑93 (MQ=255)
                                                 aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTc                                                                                                                                           <  2:6465180/93‑1 (MQ=255)
                                                 aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATa                                                                                            >  1:546320/1‑140 (MQ=255)
                                                 aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGATTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATa                                                                                            <  2:3467252/140‑1 (MQ=255)
                                                      cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCCATCCTCATCCTGAATTCCGGCCAGCATGATAAacac                                                                                       >  1:4613128/1‑140 (MQ=255)
                                                                tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCAt                                                                                                                                        >  1:2758312/1‑81 (MQ=255)
                                                                   aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                                                          <  1:5610666/140‑1 (MQ=255)
                                                                      aCCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTGTTCTTACg                                                                       >  1:6417026/1‑140 (MQ=255)
                                                                       ccTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTCATAAATCATCTGGGCCCTTCCATTTCCGAAAGACCAAACCTCATCCAGATTTAGGGCCAGCATCATAAACACATCTTCTTTTCTTACgg                                                                      >  2:5520196/1‑139 (MQ=255)
                                                                       ccTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGc                                                                      >  2:1743862/1‑140 (MQ=255)
                                                                         ttCCCATTAATAGACAACCCCGCGGGTCTCACTCCGCGGGGTGTTATTATTCAATCATATGGGCCCTTCCTTTTCCGACAGACAAATCCTCAGGCTGAGTTCCGGCCAGCATGATAATGACATCTTGTGTTCGTACgact                                                                    >  2:712846/1‑137 (MQ=255)
                                                                            ccATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCCATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCACGc                                                                 >  1:6858621/1‑140 (MQ=255)
                                                                             cATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCt                                                                <  1:4775491/140‑1 (MQ=255)
                                                                             cATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCt                                                                <  1:6584666/140‑1 (MQ=255)
                                                                              aTTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTc                                                               >  2:3313551/1‑140 (MQ=255)
                                                                                  aTAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaa                                                           >  2:7349223/1‑140 (MQ=255)
                                                                                      accaccCCGCGGGTATCCATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaa                                                       >  1:289535/1‑140 (MQ=255)
                                                                                      accaccCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGcc                                                                     >  2:5205360/1‑126 (MQ=255)
                                                                                      accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGcc                                                                     <  1:5205360/126‑1 (MQ=255)
                                                                                        caccCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     >  1:77770/1‑140 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATc                                                                                                                        <  1:5852886/73‑1 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     >  2:1243793/1‑140 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     >  1:4202868/1‑140 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     >  2:3977503/1‑140 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     <  1:4920276/140‑1 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTGTCTTACGCCAAGCTCGgaatgtata                                                     >  2:1490856/1‑139 (MQ=255)
                                                                                        caccCCGCGGGTATCAATCCGCGGGGTGTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     >  2:4820912/1‑140 (MQ=255)
                                                                                          ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCGTTCCATGTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAAAACATCTTCTTTTCTTCCCCCAAGCTCGGACTGAATCCg                                                   >  2:5029427/1‑140 (MQ=255)
                                                                                          ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACTCCAAGCTCGGAATGAATCCg                                                   >  1:2060226/1‑140 (MQ=255)
                                                                                          ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                                                   >  1:4486521/1‑140 (MQ=255)
                                                                                          ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                                                   >  2:2402288/1‑140 (MQ=255)
                                                                                           cccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCCGCATGATAAACACATCTTGTTTGCTTACGCCCAGCTCGGAAGGAATCCgg                                                  >  2:7690277/1‑140 (MQ=255)
                                                                                            ccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGc                                                 >  2:3182875/1‑140 (MQ=255)
                                                                                                    aTCAATCCGCGGGGTATTATTATTCAATCAGCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCGGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt                                         <  1:1112024/140‑1 (MQ=255)
                                                                                                     tCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACTCCAAGCTCGGAATGAATCCGGCTTGCGATTg                                        >  1:5547057/1‑140 (MQ=255)
                                                                                                     tCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTg                                        <  2:4151937/140‑1 (MQ=255)
                                                                                                       aaTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg                                      <  1:5137841/140‑1 (MQ=255)
                                                                                                       aaTCCGCGGGGTTTTATTAGTCAATCATCTGGGCCCTTACATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACCCATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg                                      <  2:6639826/140‑1 (MQ=255)
                                                                                                            gcgGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTa                                 >  2:6974460/1‑140 (MQ=255)
                                                                                                              ggggTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaaa                               <  2:6124356/140‑1 (MQ=255)
                                                                                                              ggggTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaaa                               <  2:564451/140‑1 (MQ=255)
                                                                                                                 gTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTg                            <  1:1400473/140‑1 (MQ=255)
                                                                                                                   tttATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  2:3675239/140‑1 (MQ=255)
                                                                                                                   tttATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCAGCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  2:2126644/140‑1 (MQ=255)
                                                                                                                   tttATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  2:2637069/140‑1 (MQ=255)
                                                                                                                   tttATTATTCAATCATCGGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  2:6313434/140‑1 (MQ=255)
                                                                                                                      attattCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtt                       <  1:3526613/140‑1 (MQ=255)
                                                                                                                      attattCAATCATCTGGGCCCTTCCATGTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtt                       <  1:2555579/140‑1 (MQ=255)
                                                                                                                          ttCAATCATCTGGGCGCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGACTGAATCCGGCTGGCGATTGAGCGGTAAAGTGCTCTTTTCt                   >  2:2982812/1‑140 (MQ=255)
                                                                                                                          ttCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCt                   >  1:7161347/1‑140 (MQ=255)
                                                                                                                          ttCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCt                   >  1:3256150/1‑140 (MQ=255)
                                                                                                                           tCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAAGAGATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCAGGCTTGCGATTGAGCGGTAAAGTGGTCTTTTCTg                  >  2:1053348/1‑140 (MQ=255)
                                                                                                                           tCAATCATCTGGGCCCGTCCATGTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTg                  <  2:677680/140‑1 (MQ=255)
                                                                                                                            cAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg                                      <  1:1710718/119‑1 (MQ=255)
                                                                                                                            cAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTGCTTACGCCAAGCTCGGAATGTATCCGGCTGGCGATTGAGCGGTAAAGGGCTCTTTTATGt                 >  2:5780320/1‑140 (MQ=255)
                                                                                                                                cctcTGGGCCCTTCCATTTCCGAAAGACCAATACTCATCCTGATTTCCGGCCAGCATGATAAACACCTCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  1:2255806/138‑1 (MQ=255)
                                                                                                                                catcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  2:2036775/140‑1 (MQ=255)
                                                                                                                                catcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  2:4583453/140‑1 (MQ=255)
                                                                                                                                catcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  2:4486521/140‑1 (MQ=255)
                                                                                                                                catcTGGGCCCTTCCATGTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  1:3480469/140‑1 (MQ=255)
                                                                                                                                catcTGGGCCCTTACATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  1:3804590/140‑1 (MQ=255)
                                                                                                                                catcTGGGCCCGTCCCTGTCCGAAAGACCAAGCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  1:1198688/140‑1 (MQ=255)
                                                                                                                                  tcTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAgtt           <  2:2742608/140‑1 (MQ=255)
                                                                                                                                    tGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAgttgt         >  1:6121123/1‑140 (MQ=255)
                                                                                                                                       gCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCt      >  1:879637/1‑140 (MQ=255)
                                                                                                                                          cTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGa   <  1:2345975/140‑1 (MQ=255)
                                                                                                                                          cTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGa   <  1:5947981/140‑1 (MQ=255)
                                                                                                                                          cTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGa   <  1:7452737/140‑1 (MQ=255)
                                                                                                                                           ttCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAc  >  1:4655017/1‑140 (MQ=255)
                                                                                                                                           ttCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAc  >  1:1622060/1‑140 (MQ=255)
                                                                                                                                           ttCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAc  <  2:2241727/140‑1 (MQ=255)
                                                                                                                                           ttCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGGTGTTCTTGAc  >  2:3799896/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAC  >  NZ_CP034943/3184622‑3184900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: