Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,193,442 T→G 6.6% E61D (GAA→GAC htrB ← serine protease Do‑like protein HtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,193,4420TG 6.6% 1066.9 / 12.2 406E61D (GAA→GAChtrBserine protease Do‑like protein HtrB
Reads supporting (aligned to +/- strand):  ref base T (142/220);  new base G (42/2);  total (184/222)
Fisher's exact test for biased strand distribution p-value = 7.45e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

AGAAACGGCCTCGGTCGCCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATT  >  NZ_CP034943/3193304‑3193581
                                                                                                                                          |                                                                                                                                           
aGAAACGGCCTCGGTCGCCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGttt                                                                                                                                            <  1:6984434/140‑1 (MQ=255)
 gAAACGGCCTCGGTCGCCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTc                                                                                                                                           >  2:7097159/1‑140 (MQ=255)
           cGGTCGCCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTcc                                                                                                                                 <  1:5211713/140‑1 (MQ=255)
           cGGTCGCCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTcc                                                                                                                                 <  2:3388567/140‑1 (MQ=255)
                  cTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGACTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTa                                                                                                                          <  1:6760581/140‑1 (MQ=255)
                           gTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTGCATTTGTAAATCGGAAg                                                                                                                 >  1:6660790/1‑140 (MQ=255)
                           gTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAg                                                                                                                 <  1:3780596/140‑1 (MQ=255)
                           gTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCGGTTCCATTTATAAAGCAGAAg                                                                                                                 >  1:638054/1‑140 (MQ=255)
                                aTGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTATAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTTAAGCAGAAGCCGtt                                                                                                            <  2:6543662/140‑1 (MQ=255)
                                aTGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGGGTCTTTTGCAATTCTAAAACATAAGCCggt                                                                                                            >  1:2084202/1‑138 (MQ=255)
                                aTGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATGTCTAAAGCAGAAGCCGtt                                                                                                            <  2:2116872/140‑1 (MQ=255)
                                   gCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCa                                                                                                         <  1:2601723/140‑1 (MQ=255)
                                       aTAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGtt                                                                                                     >  2:7578373/1‑140 (MQ=255)
                                           ggCGCGCTCCCTCGCAACAGACCCCCGCCGATAATCCCGCCGAAAATCGGGCTCAGCCAGGCGGCTCTCCGTTTCTTTTCTTTGTTCACAGCCGTTTCTGTCTTTTCCATGTCTAAAGCAGAAGCCGTTTCATGTTTTcc                                                                                                 <  2:6423857/140‑1 (MQ=255)
                                           ggCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTcc                                                                                                 <  1:7578373/140‑1 (MQ=255)
                                           ggCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTcc                                                                                                 <  2:1311138/140‑1 (MQ=255)
                                           ggCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTAGTGTTTTcc                                                                                                 >  2:5352633/1‑140 (MQ=255)
                                             cgcgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCg                                                                                               <  1:2944917/140‑1 (MQ=255)
                                             cgcgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCGGTCTTTTCCATTTCTAAAGCAGAAGGCGTGTGATGTTTTCCCg                                                                                               >  2:5006834/1‑140 (MQ=255)
                                             cgcgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCAATGCGGCTCTCCGTGTCTTTTCTTTTTACACAGCCGTTTGTGTCTTTTCCATTTGTAAAACAGAAGCGGTTTCATGTTTTCCCg                                                                                               >  2:7228646/1‑140 (MQ=255)
                                               cgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTGTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTGTCAGGTTTTCCCGCt                                                                                             >  2:4913775/1‑140 (MQ=255)
                                                  tGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAACTCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCa                                                                                          <  1:4649469/140‑1 (MQ=255)
                                                  tGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCa                                                                                          <  2:2007048/140‑1 (MQ=255)
                                                  tGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCa                                                                                          <  2:6881158/140‑1 (MQ=255)
                                                    ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                        <  2:3346281/140‑1 (MQ=255)
                                                    ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                        <  2:7643053/140‑1 (MQ=255)
                                                    ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                        <  1:6533746/140‑1 (MQ=255)
                                                      gAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCAGGCGGCTCTCCGTTTCTTTTCTTTTTTCACGGCCGTGTCTGTCTGTTCCATTTCTAAAGCAGAAGCCGTGTCATGTGTTCCCGCGTCagagg                                                                                      >  2:7416326/1‑136 (MQ=255)
                                                       aGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATAAGa                                                                                     >  2:3056793/1‑140 (MQ=255)
                                                       aGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCGTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCGTCATACGa                                                                                     >  1:3852537/1‑140 (MQ=255)
                                                          aTCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTACATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAAc                                                                                  >  2:4616266/1‑140 (MQ=255)
                                                              gACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGttt                                                                                                                                            <  2:5468729/78‑1 (MQ=255)
                                                              gACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGttt                                                                                                                                            >  1:5468729/1‑78 (MQ=255)
                                                                  cccGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGt                                                                          <  1:265762/140‑1 (MQ=255)
                                                                   ccgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCTTTTCTTTTCTTTTTTCACAGCCGTGTCTGTGTTTTGCATTTCTAAAGGAGAAGCCGTTTGATGTTTTCCCGCTTCATAAGAAACAGGcgcggc                                                                         >  1:2203242/1‑138 (MQ=255)
                                                                   ccgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTCTTTTCCATTTCTAAAGCAGAAACCGTTTCTTGTTTTGCCGCTTCATACGAAACAGGGGCGtg                                                                         >  1:7056210/1‑139 (MQ=255)
                                                                    cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCGTTTCCCTTTCTAAAGCAGAAGCCGTGTCATGTTTTCCGCGGTCCTACGAAACACGCGCGTcg                                                                        >  2:5895844/1‑139 (MQ=255)
                                                                    cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTGTGTGGTTTCCATTTCTAAAGCACAAGCCGGTTCATGTTTTACCGCGTCATACCCAACAGGcgcggcc                                                                        >  1:2377458/1‑137 (MQ=255)
                                                                    cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTGTGTCTTTTGCATTTCTAAAGCAGAAGCCGTTTCATGTTTTGCCGCTTCATAAGAAACAGGCGCGTCa                                                                        >  1:5765048/1‑140 (MQ=255)
                                                                       cgATAATCCCGCCTAAACTCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGc                                                                     <  1:977604/140‑1 (MQ=255)
                                                                       cgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGc                                                                     <  1:1369716/140‑1 (MQ=255)
                                                                         aTAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTGATGTTTTCCCGCTTCATa                                                                                        >  2:6041344/1‑119 (MQ=255)
                                                                         aTAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                        <  1:6041344/119‑1 (MQ=255)
                                                                         aTAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCaa                                                                   >  2:2914195/1‑140 (MQ=255)
                                                                            aTCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAAttt                                                                <  1:438451/140‑1 (MQ=255)
                                                                            aTCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAAttt                                                                <  1:5288626/140‑1 (MQ=255)
                                                                            aTCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAAttt                                                                <  1:7170933/140‑1 (MQ=255)
                                                                                 gCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTGCATTTGTAAAGCAGAAGCCGTTTCATGTTTTGCCGCTTCATAAGAAACAGGCGCGTCCAGCAATTTGTGtt                                                           >  1:3542638/1‑140 (MQ=255)
                                                                                 gCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCGTCAAACGAAACAGGCGCGTCAAGCAAATTGTGtt                                                           >  1:1731858/1‑140 (MQ=255)
                                                                                 gCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTGTGGGTTTTACAATTCTAAAGCCGAACCCGGTTCATGTTTTAGCGCGTCATACGAAACCGGCGCGTCACGCACTGTGTGtt                                                           >  1:7659668/1‑140 (MQ=255)
                                                                                  ccTAACCTCGGGCTCAGCCATGCCGCCCTCCGTTGCATTTCTTTTTTCCCAGCCGTGTCTGTCTTGTCCATTTCGACAGCCCAAGCCGGTTCATGTTTTCCCGCGTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTc                                                          <  2:5275638/140‑1 (MQ=255)
                                                                                  ccTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTc                                                          >  2:2676724/1‑140 (MQ=255)
                                                                                   cTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCa                                                         <  1:3459035/140‑1 (MQ=255)
                                                                                   cTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCa                                                         <  1:509638/140‑1 (MQ=255)
                                                                                     aaaaTCGGGCTCAGGCATGCGGCGCTCCGGTCATTTTCATATTTCCCAGCCGTTTCTGTCTTTTCCACTTCTAACGCAGAAGCCGTTTCATGTTTTCCCGCGTCATTCGAAACAGGCGCGTCAAGCAATTTCTGTTCAGt                                                       <  1:3194795/140‑1 (MQ=255)
                                                                                     aaaaTCGGGCTCAGCCATGCGGCTTTCCTTTTCTTTTCTTTTTTCACAGCAGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGt                                                       <  2:1182158/140‑1 (MQ=255)
                                                                                     aaaaTCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGt                                                       <  2:4342717/140‑1 (MQ=255)
                                                                                           gggCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTc                                                 <  1:7327745/140‑1 (MQ=255)
                                                                                                 aGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCgg                                           <  1:4771967/140‑1 (MQ=255)
                                                                                                  gCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGc                                          <  2:5421331/140‑1 (MQ=255)
                                                                                                    cATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTg                                        <  1:1206360/140‑1 (MQ=255)
                                                                                                     aTGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTAAGTATGTTCAATCGGCTGc                                       <  2:6769297/140‑1 (MQ=255)
                                                                                                      tGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCGTTTCCCTTTCTAAAGCAGAAGCCGTTTCATGTTTTGCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGcc                                      >  1:5583920/1‑140 (MQ=255)
                                                                                                        cGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTTGGGTTTGCATTTCTAAATCAGAAGGCGTTTGCTGGTTTGCCGCTTCCTAAGAAACAGGGGGGTCCAGCAAGTTTGGTTGAGGTTGTTCAATAGGGTGGCgg                                    >  1:1065683/1‑140 (MQ=255)
                                                                                                        cGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAACCCGGTTCATGTTTTGCCCCGTCATACGAAAAAGGCGCCTCAAGCAAATTGTGTTGAGTATGTTAAAAAGGCTGGCgg                                    >  1:1906827/1‑140 (MQ=255)
                                                                                                        cGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCGGTCAGTTACATTTGTAAAGCAGAAGCCGGTTCATGTTTTCCCCCGTCATAAGAAAGACGCGCGTCAAGGAAATTCTGGTGAGTATGGTTAATCGGCTGGCgg                                    >  1:3287282/1‑140 (MQ=255)
                                                                                                         ggCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  1:4573063/140‑1 (MQ=255)
                                                                                                         ggCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  2:2853009/140‑1 (MQ=255)
                                                                                                         ggCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  2:6617917/140‑1 (MQ=255)
                                                                                                         ggCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  1:393695/140‑1 (MQ=255)
                                                                                                         ggCTCTCCGTTTCTTTTCTGTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAACGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  1:5668559/140‑1 (MQ=255)
                                                                                                         ggCTCTCCGTTTCTTTTCTATTTTCACAGCCGATGCTGTCTTTTCCATTTCGAAAGCAGAAGCCGTTTCATGTTTTCCCCCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  2:2469903/140‑1 (MQ=255)
                                                                                                         ggCTCTCAGGTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                   <  2:3873361/140‑1 (MQ=255)
                                                                                                            tctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAt                                <  2:4724250/140‑1 (MQ=255)
                                                                                                            tctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCATTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAt                                <  1:2700602/140‑1 (MQ=255)
                                                                                                             ctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGt                                                                          <  1:5922393/97‑1 (MQ=255)
                                                                                                             ctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTATAAAGCAGAGGCCGGTTCATGTTTTCCCTCTTCATACGAAAAAAGCGCGTCAAGCAATTTCAGTTCAGTATGTTCAATCGGCTGCCGGTTAtt                               >  2:1599221/1‑140 (MQ=255)
                                                                                                             ctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTATAAAGCAGAAGCCGTTTCATGTTTTCCCCCTTCATACGAAACACGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAAACGGCAGCCGGTGAtt                               >  1:6922846/1‑140 (MQ=255)
                                                                                                             ctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCGGTGTTTTCCAATTCTAAATCAGAACCCGTTTCATGTTTTCCCGCTTCATACGAAAAAGGCCCGTCAAGCAATTTCTTGTGAGTATGTTAAATCGGCTGCCgggtctt                               >  1:402900/1‑135 (MQ=255)
                                                                                                              tcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAttt                              <  2:5833533/140‑1 (MQ=255)
                                                                                                              tcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAttt                              <  2:563281/140‑1 (MQ=255)
                                                                                                                 gTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                           <  1:2543821/140‑1 (MQ=255)
                                                                                                                 gTTTCTTTTCGATTTTCACAGCCGTTTCTGTCTTTTCCATTTCGAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                           <  2:4409498/140‑1 (MQ=255)
                                                                                                                   ttcttttcttTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTAtt                         <  1:3046955/140‑1 (MQ=255)
                                                                                                                     cttttcttttTTCACAGCCGTTTGTGTCTTTTGCATTTATAAAACAGAAACCGGTTAATTTTTTTCCGCGTAAAAAGAAAAAAGCGCGTCAAGCAAATTGTGTTGAGTATGTTAAAAAGGCTGGCGGGTAATTTTATTCt                       >  1:7123862/1‑140 (MQ=255)
                                                                                                                     cttttcttttTTCACAGCCGTTTCTGTGTTTTCCAATTATAAACCAGAAGCCCGTTAATTTTTTACCCCATCATAACAAAACCGCGCGTCAACCCAATTATTTTAAGTATTTTCAAACGGCTGCCGGGTATTTGTATTCt                       >  1:1650315/1‑140 (MQ=255)
                                                                                                                     cttttcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAATCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCt                       >  2:4135551/1‑140 (MQ=255)
                                                                                                                     cttttcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCACTTTCTGTTCAGTATGTTCAATCGGCTGCCGGGTATTTGTAttat                       >  2:3777131/1‑138 (MQ=255)
                                                                                                                      ttttcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTg                      <  2:6697574/140‑1 (MQ=255)
                                                                                                                      ttttcttttTTCACAGCCGTTTATGTATTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTg                      <  2:1869681/140‑1 (MQ=255)
                                                                                                                         tcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATg                   <  2:3223618/140‑1 (MQ=255)
                                                                                                                         tcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATg                   <  2:3491522/140‑1 (MQ=255)
                                                                                                                           ttttttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGtt                 <  2:6273145/140‑1 (MQ=255)
                                                                                                                            tttttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTc                <  1:7369431/140‑1 (MQ=255)
                                                                                                                            tttttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTc                <  2:4875522/140‑1 (MQ=255)
                                                                                                                             ttttCACAGCCGTTTCTGTCTTTTGCAATTCTAAATCAGAATCCGGTTAATGTTTTACCGCTTCATAACAAACACGCGCGTGAAGCAAATTCTTTTCAGGATGTTAAATAGGGTGGCGGTTATTTGTATTATTATGttat               >  1:4822162/1‑138 (MQ=255)
                                                                                                                             ttttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGGGGCCGGTTATTTGTATTCTGATGTTCt               >  2:5212394/1‑140 (MQ=255)
                                                                                                                                         tttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAt   <  2:5948866/140‑1 (MQ=255)
                                                                                                                                          ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt  <  1:4217353/140‑1 (MQ=255)
                                                                                                                                          ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTGTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt  <  1:6278624/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGAAACGGCCTCGGTCGCCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATT  >  NZ_CP034943/3193304‑3193581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: