Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 177,067 C→G 10.2% intergenic (+240/‑32) EO946_RS01020 → / → EO946_RS01025 alcohol dehydrogenase/SulP family inorganic anion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,0670CG10.2% 1003.0 / 20.0 384intergenic (+240/‑32)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base C (171/164);  new base G (46/2);  total (218/166)
Fisher's exact test for biased strand distribution p-value = 1.41e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 396 total aligned reads displayed.

CAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTT  >  NZ_CP034943/176928‑177206
                                                                                                                                           |                                                                                                                                           
cAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTAATTTCATTTCTACCGTAAAATAAATAAAGCTCCCAATAAAAAAAATACGGTGc                                                                                                                                             >  2:3713822/1‑140 (MQ=255)
              aTACTCTTGGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGGGCGGATTTATTTTTTa                                                                                                                               >  2:7371176/1‑140 (MQ=255)
                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTGATTTCTCCCGTAAAATAAATAAAGCTACCAATACAAAAAATAAGGTGGGGATTTATTTTTTTAAAAgg                                                                                                                         >  2:3257874/1‑140 (MQ=255)
                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTGATTTATCCCGGAAAATAAATAAAGCTCCCAAAACAACAAATACGGTGGGGAGTTATTTTTTAAAAAgg                                                                                                                         >  1:1410121/1‑140 (MQ=255)
                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAAACGGTGCGGATTTATTTTTTAAAAAgg                                                                                                                         >  2:1895450/1‑140 (MQ=255)
                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACATTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAAAAAGGTGCGGATTTATTTTTTTAAAAgg                                                                                                                         >  2:2558909/1‑140 (MQ=255)
                     ttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                                                        <  2:1155685/140‑1 (MQ=255)
                     ttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                                                        <  1:7424880/140‑1 (MQ=255)
                      tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTACCGTAAAATAAAAAAATCTCCCAATAAAAAAAAAACCGTGCGGATTTATTTTTTAAAAAGTAg                                                                                                                       >  1:1524579/1‑140 (MQ=255)
                      tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAATTATGCCGTAAAAAAAAGAAACCGACCCAAAAAAAAAAAACCGTGGGGATTTATTTTTTTAAAAGGAg                                                                                                                       >  2:2155668/1‑140 (MQ=255)
                            ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCAATTCGCCCGTAAAATAAAGAAAGCTCCCAATACCAAAAATAAGGTGGGGAATTATTTTTTAAAAAGGAGTCGGta                                                                                                                 >  2:3504205/1‑140 (MQ=255)
                            ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTGATTTCTCCCGTAAAAAAAATAAAGCTCCCAATAAAACAAATACCGTGGGGATTTATTTTTTTAAAAGGAGTCGTta                                                                                                                 >  2:4100816/1‑140 (MQ=255)
                            ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAAGACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTta                                                                                                                 >  2:5367808/1‑140 (MQ=255)
                             taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACACCAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTtat                                                                                                                <  1:6947188/140‑1 (MQ=255)
                               aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                              <  1:5218322/140‑1 (MQ=255)
                               aCTCCTTGCCACAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                              <  1:1185885/140‑1 (MQ=255)
                                cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTTATTTATCCCGTAAAATAAATAAAGCTCCCAAAAAAAAAAAAAAGGGGGGGCTTTATTTTTTAAAAATGAGTGGTTATAtt                                                                                                             >  1:1959257/1‑140 (MQ=255)
                                cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATATCGCCCCTAAAATAAATAAAGCACCCAAAACAACAAAAACCGTGCGGATTTATTTTTTAAAAAGGAGGAGTtataat                                                                                                             >  2:4905397/1‑138 (MQ=255)
                                  ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTCATTTGTGCCGTAAAATAAAGAAAGCGCCCCAAAAAAAAAAAAAGGGGGGGAGTTTTTTTTTATAAAGGAGTCGTTGTAtttt                                                                                                           >  1:5038943/1‑139 (MQ=255)
                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATAAGGTGGGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaat                                                                                                        >  2:4836823/1‑140 (MQ=255)
                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAAAACGGGGCGGATTTATTTTTTAAAAAGGAGTAGtttaattttaat                                                                                                        >  2:2878924/1‑140 (MQ=255)
                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCGTTGATTTGTGCCGGAAAATAAATAAATCTCCCCAAACAAAAAAAAACGGGGGGGTTTATTTTTTTAAAAGGGGTGGttttttttttat                                                                                                        >  1:5876913/1‑136 (MQ=255)
                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTAATTTATCCCCTAAAATAAAATAAGCTCCCCAAACAAAAAAAACCGTGCGGCTTTATTTTTTAAAAAGGGTGCGTTATAtttttat                                                                                                        >  1:5489412/1‑136 (MQ=255)
                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTGTTTCTCCGGTCGTTTTCCTTTCATTTGTCCCGTAAAAAAAAGAAAGCGCCCAATACAACAAAAACGGGGCGGCGTTATTTTTTAAAAAGGAGTCGTTATATATTaat                                                                                                        >  2:2944390/1‑140 (MQ=255)
                                      gCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTGTGCCGTAAAATAAAGAAAGCTCCCAATAAAACAAATACGGGGGGGTTTTATTTTTTTAAAATGAGTTGTTTTTTTTgttta                                                                                                       >  1:531684/1‑136 (MQ=255)
                                       ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                      <  1:7065951/140‑1 (MQ=255)
                                       ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                      <  2:5553904/140‑1 (MQ=255)
                                           aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTCATTTGTCCCGGAAAAAAAAAAAAGATCCCCCTAAAAAAAAAAACGGGCGGGTGTATTTTTTAAAAAAGAGTGGGTTGATTTTTataatatt                                                                                                  >  1:36123/1‑138 (MQ=255)
                                             aacaacATCGTCACCGTGTCATCATGTTCTCCGGTCGTTTTCCTTTCTTTTCTCCCGTAAAATAAAGAAAGCTCCCAATAAAAATAATTCGGTTCGGATTTATTTTTTACAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                <  2:4469721/140‑1 (MQ=255)
                                             aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                <  2:6682192/140‑1 (MQ=255)
                                                  caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCAATTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAAAAAGGGGCGGAGTTATTTTTTAAAAAGGAGGGGTTTTAGTTGAATAATAGTTTTACAt                                                                                           >  2:2024480/1‑140 (MQ=255)
                                                  caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGTAGTCGTTATATTTTAATAATATtttttaat                                                                                           >  2:7182456/1‑136 (MQ=255)
                                                     aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                                        <  1:5623087/140‑1 (MQ=255)
                                                     aCTCAACGTGTCCTCTTGTTCTCCGGTCGTTTTCCTTTCATGTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAACTACGGTGCGGATATATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                                        <  1:3794134/140‑1 (MQ=255)
                                                          aCGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTGATTTGTCCCGTAAAATAAAAAAAGCTCCCAAAAAAAAAAAAAAGGGGGGGGGTTTTTTTTTTTAAAGGTGTGGTTTTTTTTGTATAAAAGTTTTTGATTaaatgag                                                                                   >  1:1709286/1‑136 (MQ=255)
                                                            gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                                                                 <  2:1668943/140‑1 (MQ=255)
                                                            gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                                                                 <  2:7010655/140‑1 (MQ=255)
                                                            gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                                                                 <  2:3411471/140‑1 (MQ=255)
                                                             tgtCCTCTTTTTCTCCTGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTACCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                <  1:7204180/140‑1 (MQ=255)
                                                             tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                <  1:107643/140‑1 (MQ=255)
                                                             gctCCTCTTTTTCTCCGGTCGTTTTCCTTTCTTTTCTCCCGTAAAATAAAGACAGCTCCCAATACAACAAATAGGGTGCGGATTGATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                <  2:5762250/138‑1 (MQ=255)
                                                               tCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTGTCCCCTAAAATAAAAAAAGCTCCCCAAAAAAAAAATACGGGGGGGATTTATTTTTTAAAAAGGAGTGGTGTTATTTTTATAAAAGTTTTAAAATAAAGGAGCaaag                                                                              >  1:2524464/1‑138 (MQ=255)
                                                               tCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTAGGCCCTAAAAAAAAGTAAACTCCCAAATAAACAAATACGGTGGGGATTTATTTTTTAAAAAGGGGTAGTTATATTTTAATTAAAGGTTTACAATAAAGCAGCAATg                                                                              >  2:3072173/1‑140 (MQ=255)
                                                                 ctctTTTTCTCCGGTCGTTTTGCGTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAAAACGGTGCGGCTTTATTTTTTTAAAATGAGTGGTTATATGTTTAAAATAGTTTTTAAATAAAACAGCAAAGGt                                                                            >  2:2953313/1‑140 (MQ=255)
                                                                     ttttCTCCGGTCGTTTTCCTTTGCTTTGTCCCGTAAAATAAAGAAAGCTCCCAAAAAAACAAATACGGTGCGGATTTATTTTTTAAAAATGAGTCGTTATTTTTTAATAATAGTTTTTAATTAAAGCAGCAAGGGTTggg                                                                        >  2:4596407/1‑140 (MQ=255)
                                                                              gTCGTTTTGCTTTCATTTCTCCCGTAAAAAAAAGAAAGCTACCAATAAAACAAAAACGGTGCGGATTTATTTTTTAAAAAAGAGTGGTTATAATTGAAAAAAAGTTTTAGATTAAAGCAGAAATGGGTCGGGCATATCCg                                                               >  2:6595235/1‑140 (MQ=255)
                                                                              gTCGTTTTACGTTCAATTATCCCGTAAAATAAAGAAAGCGACCAAAACAACAAATAACGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATATTTTTTCAATAAAGCAGCCAAGGTTAGGGAAAATACg                                                               >  2:3595646/1‑140 (MQ=255)
                                                                                                ctcCCGTAACATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTgg                                             <  2:648636/140‑1 (MQ=255)
                                                                                                  cccGTAAAATAAAGAAAGCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCa                                           <  1:3481489/140‑1 (MQ=255)
                                                                                                  cccGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCa                                           <  1:2789348/140‑1 (MQ=255)
                                                                                                  cccGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCa                                           <  2:4545067/140‑1 (MQ=255)
                                                                                                        aaaTAAAGAAAGCTCCCAATACCACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                     <  1:6017521/140‑1 (MQ=255)
                                                                                                        aaaTAAAGAAAGCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                     <  1:3025669/140‑1 (MQ=255)
                                                                                                        aaaTAAAGAAAGCTCCCAATACAACAAATCCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                     <  2:5458477/140‑1 (MQ=255)
                                                                                                         aaTAAAGAAAGCTCCCAATACCACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGt                                    <  1:6259657/140‑1 (MQ=255)
                                                                                                         aaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGt                                    <  1:4403958/140‑1 (MQ=255)
                                                                                                         aaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGt                                    <  2:5662984/140‑1 (MQ=255)
                                                                                                           tAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTg                                  >  1:822820/1‑140 (MQ=255)
                                                                                                              agaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt                               <  2:6248745/140‑1 (MQ=255)
                                                                                                               gtaagCTCCCAATACCTCAACTTCTTTTCGCATTTTTTTTTTAAATTGTAGTGGTTATTTTTGAATAATTGTTTTACATTAAAGCAGAAATGTTTCTGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGGGtt                              <  2:5032970/138‑1 (MQ=255)
                                                                                                               gaaatCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                              <  1:961301/140‑1 (MQ=255)
                                                                                                                 aagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAg                            <  1:4522555/140‑1 (MQ=255)
                                                                                                                 aagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAg                            <  2:2455272/140‑1 (MQ=255)
                                                                                                                      cccAATACAACAAATACGGTGCTGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                       <  2:279165/140‑1 (MQ=255)
                                                                                                                           tACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAcc                  <  1:4846312/140‑1 (MQ=255)
                                                                                                                             caacaaATACGGTGCGGATTTATTTTTTAACAAGGAGTCTTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAATACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg                <  2:3003373/140‑1 (MQ=255)
                                                                                                                             caacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg                <  1:6701414/140‑1 (MQ=255)
                                                                                                                              aacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGa               <  2:4042898/140‑1 (MQ=255)
                                                                                                                               acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                     >  1:2949188/1‑117 (MQ=255)
                                                                                                                               acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAcattaa              >  2:4235455/1‑135 (MQ=255)
                                                                                                                               acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGaa              >  2:2652872/1‑140 (MQ=255)
                                                                                                                               acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAACTGaa              >  2:4773171/1‑140 (MQ=255)
                                                                                                                               acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGGTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGaa              >  2:842317/1‑140 (MQ=255)
                                                                                                                                caaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATATTTTTAAATTAAAGAAGAAATTGTTCGGGAATATACGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAg             >  1:4759163/1‑140 (MQ=255)
                                                                                                                                caaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                              <  2:4003692/123‑1 (MQ=255)
                                                                                                                                caaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                              >  1:4003692/1‑123 (MQ=255)
                                                                                                                                caaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTGATAATTGAATAAAAGTATTACATTAAAGCAGAAATTGGTATGGAATATACGAAAAGACAATCTAGCTGGGCTTTTATTTGCCATGGAATTAATACCTGaac             >  1:2252182/1‑139 (MQ=255)
                                                                                                                                 aaaTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAATCAGAAATTGTTGGGGAATATCCGAAAAGACATTCTTGCTGGGCATTTATTTTCGGTTTCATTAATACATGAAGc            >  1:4253591/1‑140 (MQ=255)
                                                                                                                                 aaaTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAtt                        <  2:7340924/128‑1 (MQ=255)
                                                                                                                                 aaaTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAtt                        >  1:7340924/1‑128 (MQ=255)
                                                                                                                                   aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACCTTAAAGCAGCACGGGTTCGGGACTATCCGAAAAGACATTCTTGCTGGCAGTTTAGTTGCGTTAGCATTAATACCTGAAGCaa          >  2:1603446/1‑140 (MQ=255)
                                                                                                                                   aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCCTTTTAGTTGCGGTTGCATTAATACCTGAAGCaa          >  1:4695893/1‑140 (MQ=255)
                                                                                                                                   aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa          >  1:5703583/1‑140 (MQ=255)
                                                                                                                                   aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTAAATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTATTTGCGTTAGCATTAATACCTGAAGCaa          >  2:4306923/1‑140 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        >  1:2021247/1‑140 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        <  1:4925671/140‑1 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        >  1:5548115/1‑140 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        >  2:5251751/1‑140 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        >  1:6362638/1‑140 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTAGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        >  2:6074589/1‑140 (MQ=255)
                                                                                                                                     aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGATTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt        <  1:4100816/140‑1 (MQ=255)
                                                                                                                                        gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc     >  2:7503212/1‑140 (MQ=255)
                                                                                                                                        gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc     >  2:6888183/1‑140 (MQ=255)
                                                                                                                                          gCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtt   <  2:1404196/140‑1 (MQ=255)
                                                                                                                                          gCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTGTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtt   <  1:3112201/140‑1 (MQ=255)
                                                                                                                                           cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATAGTTTTACATTAAAGCATCAATGGTTCGGGAATAACAGAAAATACATACTTGCTGGCAGTTGAGTTTCGTTAGCATTAATAACTGAAGCAATTGGCttt  >  1:1646367/1‑140 (MQ=255)
                                                                                                                                           cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt  >  1:5402646/1‑140 (MQ=255)
                                                                                                                                           cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt  >  1:912116/1‑140 (MQ=255)
                                                                                                                                           cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt  >  2:442088/1‑140 (MQ=255)
                                                                                                                                           cGGATTTATTTTTTAAAAAGGAGTCGTTATAGTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGAGATTCTTGCTGGGATTTTATTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt  >  1:2356932/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTT  >  NZ_CP034943/176928‑177206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: