BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,377	G→T	12.4%	Q115K (CAA→AAA)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,377	0	G	T	12.4%	955.8 / 32.9	429	Q115K (CAA→AAA)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base G (253/101); new base T (3/69); total (256/173)
Fisher's exact test for biased strand distribution p-value = 2.17e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

GTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTT  >  NZ_CP034943/3280240‑3280514

gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                         >  1:15865/1‑140 (MQ=255)

gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                         >  1:7362900/1‑140 (MQ=255)

gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                         >  1:3066511/1‑140 (MQ=255)

  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:1490955/1‑140 (MQ=255)

  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:223819/1‑140 (MQ=255)

  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:2635862/1‑140 (MQ=255)

     cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTgaaa                                                                                                                                    >  1:498237/1‑140 (MQ=255)

     cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                    >  1:4629002/1‑140 (MQ=255)

         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                >  2:3399217/1‑140 (MQ=255)

         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTGTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGGTGATTAAAAGATa                                                                                                                                >  2:5150532/1‑140 (MQ=255)

          tCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATaa                                                                                                                               >  1:3856600/1‑140 (MQ=255)

               cTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAt                                                                                                                          >  1:4598939/1‑140 (MQ=255)

                  aaTATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATACGTGAtttt                                                                                                                       >  2:4643418/1‑140 (MQ=255)

                  aaTATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAtttt                                                                                                                       >  1:3552966/1‑140 (MQ=255)

                     ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTGGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                    <  2:913674/140‑1 (MQ=255)

                     ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                    <  2:2412397/140‑1 (MQ=255)

                       acccaGCGGAATTTACTGTAAAAGAAAGTCGGTCGGTGTATTCTTTTTCCAATTTTATATTTAATTGTTAGCGCCAATCAAATTCACCAGGAGCTTTTTTTTAAAATTAATGTTTTTTAAAAGATAAGTGATTTTTATCa                                                                                                                  <  1:7115301/136‑1 (MQ=255)

                        aCAAGCGGAATTTAATGTAAATGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGTATGTTGAGTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:1203541/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTCATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:4805356/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAg                                                                                                                 >  1:6493976/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:2541932/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:2468620/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:1961404/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:4083662/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:4957608/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:1176852/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:4095086/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:1664432/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:735164/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATCTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:1048011/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGGTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:819930/1‑140 (MQ=255)

                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGGATTCGTTTTCCAAGTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAACAGATACGTGATTTTTATCAg                                                                                                                 >  1:428144/1‑140 (MQ=255)

                           aGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaa                                                                                                              >  2:4032718/1‑140 (MQ=255)

                            gggtaaTTTATTGTAAAAGAATGTCGGTCGGTGTTTTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAATTGAATGTTTATTAAAAGATATGTGATTTTTATCAGaaaa                                                                                                             <  2:5660036/136‑1 (MQ=255)

                            gctgattttaatttaaAAGAACGTGGTTCGTTTTATTGTTTTTCCATTTTTATAATTAATTGGTAGAGCCACAAAAAAACAACACGAGCCTTTTTGTAAAATTAATTTTGATTAAAATATAAGTTATTTTTATCAGaaaa                                                                                                             <  2:6717089/134‑1 (MQ=255)

                            gctgAATTTAATTTAAAAGAAAGTGGGTCGGTGTATTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCAACAAAAAATCAACCCGAGCGTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGaaca                                                                                                             <  2:6481609/137‑3 (MQ=255)

                            gcgtattttaatttaaAATAAAGTGGTTGGGTTTTTTTTTTTTCAAATTTTCTAATTAATTGTTAGAGACACTCAAAATCAACACGAGCTTTTTTGTAAATTGAATGTTTTTTAAAAGATACGTTTTTTTTATCAGaaaa                                                                                                             <  2:851946/134‑1 (MQ=255)

                            gcgtattttaatttaaAATAAAGTCGTTGGTTTTATTCTTTTTAAATTTTTATAATTATTTGTTAGAGCCACTAAAAATCAACACTAGCATTTTGGTAAAATTAATGTTGATTAAAAGATAAGTTATTTTTATAAGaaaa                                                                                                             <  2:2177878/134‑1 (MQ=255)

                            gcgtattttATTTTAAAAGAAAGTGGGTCGGTTTATTCTTTTTCCATTTTTCTAATTAATTGGTAGAGCAACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:7514921/134‑1 (MQ=255)

                            gCGTAATTTATTGTAAAAGAAAGTGGTTCTGTTTTTTCGTTTACAAATTTTCTAATTAATTGTTAGAGCCACTCAAAATCAACACGCGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTTATTTTTATCACaaaa                                                                                                             <  2:5849537/140‑1 (MQ=255)

                            gCGTAATTTAATTTAAAACAAATTCGTTCGTTTTATTCTTTTTCCAATTTTCTAATTAATTGTTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATTAATGTTGATTAAAATATAAGTTATTTTTATCACaaaa                                                                                                             <  2:1670401/140‑1 (MQ=255)

                            gCGTAATTTAATGTAAATGAACGTCGGTCGGTTTATTGGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATGATTGTTGATTAAAAGATAAGTTATTTTTATCAGaaaa                                                                                                             <  2:7135582/140‑1 (MQ=255)

                            gCGGAATTTAATTTAAAATAACGTCGGTCGTTGTATTCTTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACCCGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:6274284/140‑1 (MQ=255)

                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:5492895/1‑140 (MQ=255)

                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:2335961/1‑140 (MQ=255)

                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:1008843/1‑140 (MQ=255)

                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  2:2823943/1‑140 (MQ=255)

                            gCGGAATTTAATGAAAAACAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATGGGTAGAGCAACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAACATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:1606502/140‑1 (MQ=255)

                            gCGGAATTTAAGTTAAAACAAAGTCGGGCGTTGTATTCGTTTTCAAATTTTCTATTTAATTGGTAGAGCCAAACAAAATCACCACGAGCCTTTTTGTAAAATTAATGTTTATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:4201356/140‑1 (MQ=255)

                            ccGGAATTAACGGTAAAACAATGTCGGTCGGTGTATTCTTTTTCCAATTTTCTAATTAATTGGTAGAGCAACTAAAAAACAACACGAGCCTTTTTGAAAAATGAATGTTTATTAAAAGATACGTGATTTTTATCAGaaaa                                                                                                             <  2:5773762/139‑1 (MQ=255)

                              gtattttaatttaaACGAAATTCGGTGGTTTTTTTCTTTTTCAAATTTTCTATTTAATTGTTAGAGCCACTCAAAATCAACACTAGCCTTTTTGTAAAATTAATGTTTTTTAAAAGTTAAGTTATTTTTATCATAAAACg                                                                                                           <  2:3135014/136‑1 (MQ=255)

                              ggAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTACAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACg                                                                                                           >  1:7248611/1‑140 (MQ=255)

                                 aTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTGTCTAATTAATTGGTAGAGACACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGAcc                                                                                                        >  2:2753983/1‑140 (MQ=255)

                                            aagaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCATATACCGACt                                                                                             >  2:3317654/1‑140 (MQ=255)

                                                  gtcggtcgGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTgaagaa                                                                                       >  2:3004601/1‑140 (MQ=255)

                                                      gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                                                                                   >  2:552596/1‑140 (MQ=255)

                                                      gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                                                                                   >  2:3805403/1‑140 (MQ=255)

                                                        cggtGTATTCGTTTTCCAATTGTCTAATTAATTGGTAGAGCCCCTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAGAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGACGACAAAAgg                                                                                 >  1:6284989/1‑140 (MQ=255)

                                                        cggcGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAgg                                                                                 >  1:4141157/1‑140 (MQ=255)

                                                            gtATTCGTTTTCAAATTTTCTAATTAATTGTTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGTTTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaa                                                                             <  2:6003939/140‑1 (MQ=255)

                                                             ttttCTTTTTCAATTTTTATATTTATTTGTTATACCAAATAAAAATCAACACGAGCTTTTTTTTAAAATTATTTTTTATTAAAATATAAGTTTTTTTTATCAAAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                            <  1:604795/138‑1 (MQ=255)

                                                             tATTCTTTTTCCAATTTTCTAATTAATTGTTAGAGCAACTAAAAATCAACACGCGCCTTTTTTTAAAATTAATGTTGATTAAAAGATAATTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                            <  2:6835097/140‑1 (MQ=255)

                                                             tATTCTTTTTCCAATTTTATAATAAATTGTTAGAGCCAATCAAAATCAACCCGAGCCTTTTTGTAAAATGATTGTTGTTTAAATGTTACTTTATTTTTATAATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                            <  1:5859131/140‑1 (MQ=255)

                                                                 cGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACCCGAGCATTTTTTTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGgt                                                                        <  2:1769888/140‑1 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  2:2929306/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGTAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:3245598/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:447449/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:6809993/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:5428678/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:7584740/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:2758613/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  2:358153/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:2637168/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  2:1094440/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  2:6997562/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTGTTTGTAAAATGAATGTAGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCTACTGAAGAAAAAATGCAAAAATGTGTgg                                                                    >  1:4870822/1‑140 (MQ=255)

                                                                     ttCCAATTTTCTAATTAATAGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAAGGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACACAAGAAAAAAGGCCAAAAGGTgggg                                                                    >  1:5187584/1‑140 (MQ=255)

                                                                      tCCAATTTTCTAATTAATGGGTAGAGCCACTCAAACTCACCCCGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGt                                                                   <  2:5258153/140‑1 (MQ=255)

                                                                             ttCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgtt                                                            >  2:1222398/1‑140 (MQ=255)

                                                                              tCTAATTAATTGTTAGAGCCACACAAAATCAACCCGAGCCTTTTTGTAAAATTAATGTTGATAAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                                                           <  2:500387/140‑1 (MQ=255)

                                                                                    taattGGTAGACCCACTAAAAATCAACACGACCCTTTTTGTAAAATGAATGTTTATAAAAAGAAACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCtcttc                                                     <  1:1904034/140‑1 (MQ=255)

                                                                                            agagCCAATCAAAAACAACCCCAGCCTGTTTTTAAAATGAATTTTGATTAAAACATACGTGATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAACGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  1:673703/140‑1 (MQ=255)

                                                                                            agagCAACTCAAAATCAAAACGAGCCTTTTTTTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  2:7584740/140‑1 (MQ=255)

                                                                                            agacCCAATCAAAATCACCCCGAGCCTTTTTGTAAAATGAATTTTGATTAAAAGATACTTGATTTTTATCAGACAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  1:1538622/140‑1 (MQ=255)

                                                                                            acagCCACTCAAAATCAACACGCCCCTTTTTGTAAAATCAATGTTGATTAAAAGATAAGTCATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  2:6116636/138‑1 (MQ=255)

                                                                                            acacccACACAAAATCACCCCGCGCCTTTGTTTAAAATTAATTTTGATTAAAAGATAAGTTATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  1:6922081/136‑1 (MQ=255)

                                                                                              ctccACTAAAAATCAACACGCGCCTTTTTGTAAAATGATTGTTTATTAAACGATACGTGATTTTTATCAGAAAACGACCATATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATa                                           <  2:4471290/138‑1 (MQ=255)

                                                                                                  aCTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTc                                       >  1:4709317/1‑140 (MQ=255)

                                                                                                  aCTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTc                                       >  1:968256/1‑140 (MQ=255)

                                                                                                   cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                      >  1:4003433/1‑140 (MQ=255)

                                                                                                   cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                      >  1:2831155/1‑140 (MQ=255)

                                                                                                    tCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCaa                                     >  2:435171/1‑140 (MQ=255)

                                                                                                          ccAACACTCGCCTTTTTTTAAAATCTATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                               <  1:3641921/139‑1 (MQ=255)

                                                                                                             acacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGGTCTTGTTGTTGCATACTTCAAAATAGCCTg                            >  2:5188722/1‑140 (MQ=255)

                                                                                                                        tttGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTgc                 >  1:1295930/1‑140 (MQ=255)

                                                                                                                            tAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             >  1:3381948/1‑108 (MQ=255)

                                                                                                                               aatgaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCttttt          >  1:1810290/1‑140 (MQ=255)

                                                                                                                               aatgaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCttttt          >  2:2111555/1‑140 (MQ=255)

                                                                                                                                  gactgTTGATTAAACGAGACGTCATTTTTATCACAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtttttttt       <  1:2174447/137‑1 (MQ=255)

                                                                                                                                       ttGATTAAAAGATAAGTGATTTTTATAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCtt  <  2:2466302/140‑1 (MQ=255)

GTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTT  >  NZ_CP034943/3280240‑3280514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`