BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,491	T→C	5.5%	I304V (ATT→GTT)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,491	0	T	C	5.5%	2675.1 / 99.0	866	I304V (ATT→GTT)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base T (351/464); new base C (31/17); total (383/483)
Fisher's exact test for biased strand distribution p-value = 4.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 866 total aligned reads displayed.

GATGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAT  >  NZ_CP034943/3285354‑3285629

  tGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCt                                                                                                                                        >  1:2966732/1‑140 (MQ=34)

    gCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAAAGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTGTGTGTTAATAGCttt                                                                                                                                      >  2:4506784/1‑140 (MQ=21)

    gCGGTCGTGGTTCGATCAATGCGGTGAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTGTGTGTTAATAGCttt                                                                                                                                      >  2:5969083/1‑140 (MQ=255)

       gTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTg                                                                                                                                   >  1:7457860/1‑140 (MQ=34)

       gTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTg                                                                                                                                   >  2:4386136/1‑140 (MQ=34)

       gTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTg                                                                                                                                   >  1:2736305/1‑140 (MQ=34)

        tCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  <  1:5501869/140‑1 (MQ=34)

        tCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  <  1:4271794/140‑1 (MQ=34)

          gTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATa                                                                                                                                <  2:3752034/140‑1 (MQ=34)

               tCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  1:6882197/140‑1 (MQ=34)

               tCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  1:4803066/140‑1 (MQ=34)

               tCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGAGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  2:3309917/140‑1 (MQ=21)

                 gATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         <  2:4969376/140‑1 (MQ=34)

                 gATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         <  2:2348468/140‑1 (MQ=34)

                 gATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         <  2:7007425/140‑1 (MQ=34)

                 gATCAATGCGGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         <  2:283706/140‑1 (MQ=21)

                  aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAg                                                                                                                        >  2:4745298/1‑140 (MQ=34)

                      aTGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGt                                                                                                                    <  2:107540/140‑1 (MQ=34)

                       tGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  2:481726/1‑140 (MQ=34)

                       tGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  2:7452157/1‑140 (MQ=34)

                            ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  1:6014412/1‑135 (MQ=34)

                            ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:1669625/1‑140 (MQ=34)

                            ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:7135510/1‑140 (MQ=34)

                            ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  1:4543816/1‑140 (MQ=34)

                            ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGAGGGACATGCTCATGGTAATGCCTATTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:5071005/1‑140 (MQ=21)

                             tAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATAcaca                                                                                                             <  2:4283253/140‑1 (MQ=34)

                                 aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   <  1:1015288/130‑1 (MQ=35)

                                 aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  2:7216008/1‑140 (MQ=34)

                                 aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTGTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  2:1292197/1‑140 (MQ=21)

                                    gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  >  1:3374386/1‑112 (MQ=35)

                                    gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  2:391210/1‑140 (MQ=34)

                                    gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  2:640472/1‑140 (MQ=34)

                                     aaCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTg                                                                                                     >  2:5525815/1‑140 (MQ=34)

                                      aCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTgg                                                                                                    >  2:4622563/1‑140 (MQ=34)

                                       cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:4658841/140‑1 (MQ=34)

                                       cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:5053786/140‑1 (MQ=34)

                                       cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  2:2898743/140‑1 (MQ=34)

                                       cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:7158074/140‑1 (MQ=34)

                                           tCAGACTTTAGTGAGTAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               <  1:1164270/140‑1 (MQ=21)

                                           tCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               <  1:6649715/140‑1 (MQ=34)

                                           tCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               <  2:2165615/140‑1 (MQ=34)

                                           tCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               <  2:6275414/140‑1 (MQ=34)

                                            ccgACTTTAGTGAGGAATGACACGATTCCATGTAGGCATTATCAGCGGGCGGCCCTTTGCGGGACATGCTCATGGTAATGCGTTTGGTGATAATAGCGTTCTGATACGCGTAAGATGTATACACAGAACCGTGGTCACTa                                                                                              >  2:7305063/3‑140 (MQ=11)

                                            cAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTAGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTAGGTCACTa                                                                                              >  1:1666645/1‑140 (MQ=21)

                                               aCTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGt                                                                                           >  2:7635710/1‑140 (MQ=34)

                                               aCTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGt                                                                                           >  2:681081/1‑140 (MQ=34)

                                                     gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  2:907147/140‑1 (MQ=34)

                                                     gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  1:2432555/140‑1 (MQ=34)

                                                     gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  2:5622227/140‑1 (MQ=34)

                                                     gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  2:5787102/140‑1 (MQ=34)

                                                     gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  2:6893038/140‑1 (MQ=34)

                                                      tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  1:4439477/140‑1 (MQ=34)

                                                      tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  1:6485711/140‑1 (MQ=34)

                                                      tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  1:5587858/140‑1 (MQ=34)

                                                         ggAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGGTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGt                                                                                 >  2:1812097/1‑140 (MQ=21)

                                                             tGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTtctc                                                                             <  2:2753988/140‑1 (MQ=34)

                                                                  cGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCa                                                                        <  1:2048754/140‑1 (MQ=34)

                                                                     ttCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAgtg                                                                     >  2:6667096/1‑140 (MQ=34)

                                                                            gAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAg                                                              <  2:4066505/140‑1 (MQ=32)

                                                                            gAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAg                                                              <  2:2487102/140‑1 (MQ=32)

                                                                                  ttATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAtt                                                        <  1:6304757/140‑1 (MQ=32)

                                                                                  ttATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAtt                                                        <  1:5230085/140‑1 (MQ=32)

                                                                                  ttATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAtt                                                        <  1:1297680/140‑1 (MQ=32)

                                                                                      cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  2:3196896/140‑1 (MQ=32)

                                                                                      cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:2572314/140‑1 (MQ=32)

                                                                                      cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  2:3449388/140‑1 (MQ=32)

                                                                                      cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:890580/140‑1 (MQ=32)

                                                                                      cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:4007896/140‑1 (MQ=32)

                                                                                      cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:6179338/140‑1 (MQ=32)

                                                                                         cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                 >  2:833650/1‑140 (MQ=34)

                                                                                         cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                 >  1:4118645/1‑140 (MQ=34)

                                                                                           ggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  1:6994682/1‑72 (MQ=34)

                                                                                               tCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  2:1375851/84‑1 (MQ=33)

                                                                                               tCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGCTCACTCTGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTaat                                           >  1:3883295/1‑139 (MQ=19)

                                                                                               tCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGGATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAg                                           >  2:1500754/1‑140 (MQ=21)

                                                                                                 ccTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                         <  2:115378/140‑1 (MQ=34)

                                                                                                 ccTGTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGAGACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                         <  2:6208684/140‑1 (MQ=21)

                                                                                                         ggACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCtg                                 <  1:1531916/140‑1 (MQ=34)

                                                                                                             aTGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                             <  2:4153210/140‑1 (MQ=34)

                                                                                                                         aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 >  2:5198033/1‑140 (MQ=39)

                                                                                                                         aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 <  2:2035680/140‑1 (MQ=39)

                                                                                                                          tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTCTGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  2:2984544/1‑140 (MQ=255)

                                                                                                                          tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAt                                                         >  1:3686857/1‑99 (MQ=33)

                                                                                                                          tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  1:3175468/1‑140 (MQ=40)

                                                                                                                          tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  1:1388827/1‑140 (MQ=40)

                                                                                                                          tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTATAAGACAAAGTCTGTGTCCTGCTTATCCCCa                <  1:1874583/140‑1 (MQ=255)

                                                                                                                               tttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:2604223/1‑140 (MQ=255)

                                                                                                                               tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:6612637/1‑140 (MQ=255)

                                                                                                                                ttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:681081/140‑1 (MQ=255)

                                                                                                                                ttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:5951501/140‑1 (MQ=255)

                                                                                                                                ttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:4229887/140‑1 (MQ=255)

                                                                                                                                ttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATCAAGTGTGTCTAAGACAAAGTATGTGTCCTGCTTATCCCCAATCGTa          <  1:966140/140‑1 (MQ=11)

                                                                                                                                 tgtgTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa         >  1:7583935/1‑140 (MQ=255)

                                                                                                                                 tgtgTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa         >  1:7349289/1‑140 (MQ=255)

                                                                                                                                 tgtgTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGCaa         <  1:4336847/140‑1 (MQ=255)

                                                                                                                                 tgtgTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa         >  1:22435/1‑140 (MQ=255)

                                                                                                                                     ttAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGc     >  1:5016533/1‑140 (MQ=255)

                                                                                                                                      tAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa    >  1:5734954/1‑140 (MQ=255)

                                                                                                                                        aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  <  2:1949149/140‑1 (MQ=255)

                                                                                                                                        aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  <  1:7256465/140‑1 (MQ=255)

GATGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAT  >  NZ_CP034943/3285354‑3285629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`