BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,567	C→T	32.6%	L278L (CTG→CTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,567	0	C	T	32.6%	1933.3 / inf	1381	L278L (CTG→CTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base C (458/471); new base T (242/207); total (700/681)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 1387 total aligned reads displayed.

TGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTC  >  NZ_CP034943/3285428‑3285704

     gCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTg                                                                                                                                      <  1:2676524/140‑1 (MQ=32)

        ttATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAtt                                                                                                                                   <  1:6304757/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  1:5926680/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  2:3449388/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  1:4319840/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  2:6705395/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  1:7406080/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  1:3438155/140‑1 (MQ=32)

            cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                               <  1:890580/140‑1 (MQ=32)

             aGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTa                                                                                                                              >  2:2408798/1‑140 (MQ=33)

               cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                                                                                            >  1:4118645/1‑140 (MQ=34)

               cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                                                                                            >  1:1594970/1‑140 (MQ=34)

               cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGGGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                                                                                            >  2:4344314/1‑140 (MQ=21)

                 ggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTc                                                                                                                          >  2:3316064/1‑140 (MQ=34)

                       ccTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                                                                                                    <  2:4596451/140‑1 (MQ=34)

                       ccTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                                                                                                    <  2:4090692/140‑1 (MQ=34)

                       ccTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                                                                                                    <  2:6876063/140‑1 (MQ=34)

                         tttGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                                                                                                  <  1:6201482/140‑1 (MQ=34)

                            gCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGt                                                                                                               <  2:6921324/140‑1 (MQ=34)

                            gCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATGAAGTATGTCTAAGACAAAGt                                                                                                               <  2:6247053/140‑1 (MQ=21)

                            gCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTAGGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGt                                                                                                               <  1:6839522/140‑1 (MQ=21)

                                               aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTCTGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAATACAAAGTCTGTGTCCTGCTTATcccc                                                                                            >  2:2975537/1‑140 (MQ=255)

                                                tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAt                                                                                                                                    <  2:910337/99‑1 (MQ=33)

                                                 gCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCaa                                                                                          >  2:1446103/1‑140 (MQ=40)

                                                     tttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt                                                                                      >  2:7336986/1‑140 (MQ=255)

                                                       tgtgTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa                                                                                    <  2:1787131/140‑1 (MQ=255)

                                                       tgtgTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa                                                                                    >  1:6964738/1‑140 (MQ=255)

                                                              aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                             <  1:6680846/140‑1 (MQ=255)

                                                              aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                             <  1:1286210/140‑1 (MQ=255)

                                                              aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                             <  2:1949149/140‑1 (MQ=255)

                                                                   tttCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                               >  2:6619949/1‑133 (MQ=255)

                                                                     tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTGATCCCCAATCGTAAAAGCAATCACTtct                                                                      >  2:3976911/1‑140 (MQ=255)

                                                                     tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                      >  1:6539428/1‑140 (MQ=40)

                                                                     tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                      >  1:3578298/1‑140 (MQ=40)

                                                                        gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTACCACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   <  1:5750836/140‑1 (MQ=255)

                                                                        gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   <  2:472302/140‑1 (MQ=255)

                                                                        gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   <  2:7171416/140‑1 (MQ=255)

                                                                        gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   <  1:4835455/140‑1 (MQ=40)

                                                                             tCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGta                                                              <  2:3143820/140‑1 (MQ=40)

                                                                              agTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTTTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtat                                                             <  2:4473082/139‑1 (MQ=255)

                                                                                tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                           <  1:1090207/140‑1 (MQ=40)

                                                                                    aTGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCt                                                       >  1:100910/1‑140 (MQ=255)

                                                                                    aTGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCt                                                       >  1:3175523/1‑140 (MQ=255)

                                                                                    aTGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCt                                                       >  1:1726283/1‑140 (MQ=255)

                                                                                     tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTa                                                      <  2:1035769/140‑1 (MQ=255)

                                                                                       tataCACAGAACCTTGGTCACTCTGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCCTTGTATAAATCTAat                                                    >  2:535224/1‑140 (MQ=11)

                                                                                       tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                    >  1:138188/1‑140 (MQ=255)

                                                                                       tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                    >  2:1130425/1‑140 (MQ=255)

                                                                                        ataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAata                                                   <  2:166836/140‑1 (MQ=255)

                                                                                         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAatat                                                  >  2:22886/1‑140 (MQ=255)

                                                                                         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGAGTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAatat                                                  >  2:795574/1‑140 (MQ=255)

                                                                                              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                             >  1:3031425/1‑140 (MQ=255)

                                                                                              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                             >  1:4252256/1‑140 (MQ=255)

                                                                                              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                             >  2:25965/1‑140 (MQ=255)

                                                                                              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                             >  2:128710/1‑140 (MQ=255)

                                                                                              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                             >  1:728056/1‑140 (MQ=255)

                                                                                               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGa                                            >  1:1515214/1‑140 (MQ=255)

                                                                                               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                        <  2:5812246/112‑1 (MQ=255)

                                                                                                caCCCTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTGGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                           <  1:4418826/139‑1 (MQ=255)

                                                                                                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                           <  2:5141181/140‑1 (MQ=255)

                                                                                                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                           >  2:2685588/1‑140 (MQ=255)

                                                                                                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                      <  1:3882303/113‑1 (MQ=255)

                                                                                                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                           <  1:5117633/140‑1 (MQ=255)

                                                                                                aaCCGTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                           <  1:4013330/140‑1 (MQ=255)

                                                                                                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                        <  2:2825742/140‑1 (MQ=255)

                                                                                                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtat                                                             <  2:5523182/117‑1 (MQ=255)

                                                                                                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGtc                                      >  1:6419662/1‑139 (MQ=255)

                                                                                                          aCTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGAAAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCAATTGTATAAATCTAATATACTGGAAAGGTACAAtt                                 >  1:7138634/1‑140 (MQ=11)

                                                                                                             aTGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGta                                                              <  2:6134723/108‑1 (MQ=255)

                                                                                                                tAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCt                           <  1:2469605/140‑1 (MQ=255)

                                                                                                                 aaCACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCCTTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTg                          >  1:778941/1‑140 (MQ=255)

                                                                                                                 aaCACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTg                          <  1:2853605/140‑1 (MQ=255)

                                                                                                                  acacGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATc                                                        <  2:3853050/109‑1 (MQ=255)

                                                                                                                     cGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATCTACTGGAAAGGTACAATTGTTTCTGTCCa                      <  1:2270757/140‑1 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                           >  2:1578821/1‑118 (MQ=11)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                     >  2:2083429/1‑140 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGGTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTAAAATTGTTTCTGTCAAt                     >  2:4229262/1‑140 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   >  2:3749040/1‑94 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   <  1:3749040/94‑1 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAata                                                   <  1:2036077/110‑1 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                           >  1:6056524/1‑118 (MQ=255)

                                                                                                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                     >  1:7579202/1‑140 (MQ=255)

                                                                                                                       cAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                    <  2:5979345/140‑1 (MQ=255)

                                                                                                                       cAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                    <  1:4506784/140‑1 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   >  2:5001113/1‑92 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                   <  1:5001113/92‑1 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                   >  1:4589567/1‑140 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                               <  2:3593810/80‑1 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                           <  2:323487/100‑1 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                   >  2:4911701/1‑140 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                   >  1:5577034/1‑140 (MQ=255)

                                                                                                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                   >  1:545646/1‑140 (MQ=255)

                                                                                                                          ttCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                 >  2:6398113/1‑140 (MQ=255)

                                                                                                                               aGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat            >  2:7008544/1‑140 (MQ=255)

                                                                                                                                   aGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgt        >  1:5324386/1‑140 (MQ=255)

                                                                                                                                    gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGt                                       >  2:754838/1‑108 (MQ=11)

                                                                                                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg       <  1:1901049/140‑1 (MQ=255)

                                                                                                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg       >  1:4712743/1‑140 (MQ=255)

                                                                                                                                       ttGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATg    <  1:3279672/140‑1 (MQ=255)

                                                                                                                                        tGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGt   <  2:5638502/140‑1 (MQ=255)

TGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTC  >  NZ_CP034943/3285428‑3285704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`