Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,319,761 T→G 5.0% pseudogene (544/1292 nt) EO946_RS17170 ← arabinogalactan endo‑beta‑1,4‑galactanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,319,7610TG 5.0% 1256.8 / 7.1 477pseudogene (544/1292 nt)EO946_RS17170arabinogalactan endo‑beta‑1,4‑galactanase
Reads supporting (aligned to +/- strand):  ref base T (194/233);  new base G (44/2);  total (240/237)
Fisher's exact test for biased strand distribution p-value = 4.25e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 526 total aligned reads displayed.

AATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAG  >  NZ_CP034943/3319625‑3319900
                                                                                                                                        |                                                                                                                                           
    ccTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGatcat                                                                                                                                      <  2:2422134/140‑1 (MQ=255)
     cTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGatcatc                                                                                                                                     >  2:5634605/1‑140 (MQ=255)
        ggTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTTATGCTCttt                                                                                                                                  >  2:7018326/1‑140 (MQ=255)
           gtgtctCCCTGACCGCGCGGCTTCCTTAATTAAATAATTGGCTCATTTTCTCCCAATCGGTTTCACCTGCAAATGCCCATGTCGTTTCATTTCCGACGTGTACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGc                                                                                                                               <  2:1784385/139‑1 (MQ=255)
           ctgtctCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGc                                                                                                                               <  1:5243290/140‑1 (MQ=255)
           ctgtctCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGATCATCTTTTGc                                                                                                                               <  2:1538456/140‑1 (MQ=255)
              tctcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGATCATCTTTTGCAgg                                                                                                                            >  2:1622185/1‑140 (MQ=255)
                tcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          <  1:4594102/140‑1 (MQ=255)
                tcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          <  1:1920402/140‑1 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        <  2:2945626/140‑1 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        <  2:7644880/140‑1 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        <  1:2802442/140‑1 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        <  2:3455719/140‑1 (MQ=255)
                  ccTGACCGCGCGGCGGCCGCCATGACAGAATTGGCTCCTCTTCGCCCAATCGGCTTCACCTGCAACTGCCCCTGTCGTTTCCTTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCATTTGCAGGCttt                                                                                                                        <  2:2757700/140‑1 (MQ=255)
                      aCCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGttt                                                                                                                    <  1:3641066/140‑1 (MQ=255)
                        cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCg                                                                                                                  <  1:2669395/140‑1 (MQ=255)
                        cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTATCATTTCCGACCTGAAACAAGCCCATGTCGACGGCTTGCTGGTGCATGTTTTGCAGGgtttgtttgg                                                                                                                  >  1:5120034/1‑138 (MQ=255)
                        cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGATTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACCCCTTCCTTGATGATCTTTTGCAGGCTTTGTTTCg                                                                                                                  >  2:5369605/1‑140 (MQ=255)
                          cgcgGGTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACGTGCAAATGCCCCTGTCGTTGGATTTGCGCCCTGAACCATGGCGATGTCGACGCCTTCCGGGATCATGTTTTGCAGGGTTTGTTTGGta                                                                                                                >  2:190084/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTGATTTCCGGCCTGAACCATGCCCATGTCGACGCCGTCCGGTTTCAGCTTTTTCAGGCGTTGTTTCGta                                                                                                                >  1:1227697/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          <  2:5549648/130‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  2:3842901/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  1:54207/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  1:7649241/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACCCCTTCCGTGATCAACATTTGCAGGCGTTGTTTCGta                                                                                                                >  1:3830085/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCAAGCCAAAGTCGACGCCGTCCTTGATCATCTTTTTCAGGCGTTGTTTCGta                                                                                                                >  1:593875/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGAACTGAACCATGCCAAATTAGAAGCCTTACTTGAACATCTTTTTCAGGCTTTGTTTCGta                                                                                                                >  1:1489037/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGAACTGAAACATGCCAAAGTCGACGCCGTACTTGATGATCTTTTGCAGGCTTTGTTTCgga                                                                                                                >  1:2588259/1‑138 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAATTCCGACCTGAACCATGCCAAAGTCGACCCCTTCCTTGTTCAACTTTTTCAGGCTTTGTTTAGta                                                                                                                >  1:3381603/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAATTACGACCTGAACCATGCCCAAGTCGACGCCGTCCTTGATCATCTTTTTCAGGCTTTTTTTAGta                                                                                                                >  1:5626899/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCACTGTCGTTTCATTTCAGACCTGAACCATGCATATTTATAAGCATTCCTTGATCTTCTTTTGAAtgttttgtttttta                                                                                                                <  1:839190/140‑5 (MQ=255)
                           gctgCTTCCTTCATTAAATTATTTGCTCATTTTCGCCCAATGGGTTTCACCTGCTAATGGCGCTGTAGTTTCATTTCCGTCCTTAAGGTTTCCATTTTCTACGGGTTCATTGATCATCTTTTGCAGGCTTTTTTTCGtat                                                                                                               <  2:4105657/137‑1 (MQ=255)
                           gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                                                               <  1:3022070/140‑1 (MQ=255)
                           gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                                                               <  2:7392266/140‑1 (MQ=255)
                           gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                                                               <  1:5659234/140‑1 (MQ=255)
                           gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                                                               <  2:6213258/140‑1 (MQ=255)
                           gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                                                               <  2:5840630/140‑1 (MQ=255)
                                                ttGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCCTGCCAATGTCGACGCCTTGCTTGATCCTCTTTTTCAGGCTTTGTTTAGTATACTCAGAGAGCGTTGtttttt                                                                                          >  2:361091/1‑140 (MQ=255)
                                                ttGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCGGAACCATGCCAATGTCGACGCCTGCCGTGTTCATCGTTTGCAGGCTTTGTTTCGTATACTCATATAGCTTTGtttttt                                                                                          >  1:1008479/1‑140 (MQ=255)
                                                ttGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCAGTTCCGACCAGAACCATGCCAATGTCGACGCCGTCCTTGATAATCTTTTTCAGGCTTTGTTTCGTATACTCATAGAGCTTTGtttttt                                                                                          >  2:5152161/1‑140 (MQ=255)
                                                 tGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTGCGACCTGAACCATGCCCATGTCGACGCCTGCCGTGTTCAGCTTTTGGAGGGGTTGTTTCGTATAATCATAGAGGTTTGttttttt                                                                                         >  1:3218156/1‑140 (MQ=255)
                                                      cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCATGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                                                                    <  2:4825126/140‑1 (MQ=255)
                                                      cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                                                                    <  1:4155150/140‑1 (MQ=255)
                                                      cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                                                                    <  2:335068/140‑1 (MQ=255)
                                                      cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                                                                    <  1:1376485/140‑1 (MQ=255)
                                                        ttttCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGATCATCTTTTGCAGGCGTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTTGCTTCa                                                                                  >  2:7532787/1‑140 (MQ=255)
                                                             gCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCt                                                                             <  2:6727701/140‑1 (MQ=255)
                                                             gCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCt                                                                             <  1:7141868/140‑1 (MQ=255)
                                                             gCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCt                                                                             <  1:3511384/140‑1 (MQ=255)
                                                                       tttCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGcc                                                                   <  2:7170637/140‑1 (MQ=255)
                                                                        ttCACCTGCAAATGCCCCTGTCGTTTCATTTCCGCCCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCCTAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGccc                                                                  <  1:3599063/140‑1 (MQ=255)
                                                                          cACCGGCACATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCCTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCaa                                                                <  2:4243236/140‑1 (MQ=255)
                                                                            ccTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCCTGCCCATGTCGACGCCGTCCGTGATCATGTTTTGCAGGCGTTGTTTGGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGc                                                              >  1:4318682/1‑140 (MQ=255)
                                                                              tGCAACTGCCCCTGACGTTTCATTTCCGACCTGAACCAAGCCAATGTCGACGCCTGCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTTAGCTTCGCCCAAGCtt                                                            <  1:5790722/140‑1 (MQ=255)
                                                                              tGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCtt                                                            <  2:5208496/140‑1 (MQ=255)
                                                                               gCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATGTTTTGCAGGCGTTGTTTGGTATACTCATAGAGCTTTGTTTTTTTAGGTTCAAAGCTGAGCTTAGCCCCAGCttt                                                           >  2:6883712/1‑140 (MQ=255)
                                                                               gCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCAAGCCAATGTCGACGCCTTCCGTGCTCATCTTTTGCAGGGGTTGGTTCGTATAGTCATAGAGCGTGGTTTTTTTAGCTTCAAAGCTGTGCTTCGCCCAAGCttt                                                           >  2:5072917/1‑140 (MQ=255)
                                                                                 aaaTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTGGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTgg                                                         <  2:4310557/140‑1 (MQ=255)
                                                                                 aaaGGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCAAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTgg                                                         <  2:2074716/140‑1 (MQ=255)
                                                                                   atcctccTGTTGTTTCATTTCAGACCTGAACCATGCCGATGTCAACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGCTTTTTTTGCTTCAAAGCTGGGATTCGCCCAGGCTTTgggc                                                       <  2:901054/135‑2 (MQ=255)
                                                                                   aTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGt                                                       <  1:1750121/140‑1 (MQ=255)
                                                                                   aTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGt                                                       <  1:5054826/140‑1 (MQ=255)
                                                                                   aTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCCTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGt                                                       <  2:5992773/140‑1 (MQ=255)
                                                                                      ccccTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGGCGTCCTTGGTGCTCTTTTTGAGGGGGTGTTTGGTTTAATCATAGTGGTTTGTTTTTTTATGTTGAAAGCTGTGGTTAGCCCAACCGTTTGGTAcc                                                    >  1:2115693/1‑140 (MQ=255)
                                                                                          tGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTGCTTGATCATCTTTTGCAGGCGTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCGTCGCCCAAGCTTTGGGTACCTTCt                                                >  2:1428208/1‑140 (MQ=255)
                                                                                           gTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTg                                               >  2:4592503/1‑140 (MQ=255)
                                                                                           gTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTg                                               <  2:7122946/140‑1 (MQ=255)
                                                                                                 taaTTTCCGACCTGAACCATGCCAAAGTGGACGCCGTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCGTTGTTTTTTTAGCTTCAAAGCTGATCTTCGCCCCAGCGTTGGGTACCTTCTGTTTCGc                                         >  2:3194323/3‑140 (MQ=255)
                                                                                                      tCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  2:1513735/140‑1 (MQ=255)
                                                                                                      tCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  2:5723794/140‑1 (MQ=255)
                                                                                                      tCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  2:3772951/140‑1 (MQ=255)
                                                                                                      tCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTGAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  1:6263851/140‑1 (MQ=255)
                                                                                                      tCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  1:746188/140‑1 (MQ=255)
                                                                                                      tCCGACCAGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGTCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  2:1089935/140‑1 (MQ=255)
                                                                                                          aCCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGc                                >  1:6712813/1‑140 (MQ=255)
                                                                                                             tGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTAGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCa                             <  1:7295751/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAAt                        <  2:6395013/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCTTCCTTGATCATCTTTGGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAAt                        <  2:4725408/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCTTCCTGGATCATCTTGTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTACTGTTTCGCAGGATCGGCCCAGAAAt                        <  2:2557284/140‑1 (MQ=255)
                                                                                                                    tGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTGTCGTATAGTCATCGAGGTTTGTTTGTTTAGCTTCAAAGCTGAGCTTCGGCCACGCTGGGGGTCGCTGCTGTTTCGCAGGAGCGGGCCAGAACTcg                      >  1:4018599/1‑139 (MQ=255)
                                                                                                                     gCCAATGTCGACGCCTTCCTTGATCAGCTTTTGCAGGCTTTGTTTCGTATACTCATATAGCTATGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCTCAGGATCGGCCCAGAAATCAg                     >  1:1861476/1‑140 (MQ=255)
                                                                                                                     gCCAATGTCGACGCCTTCCTGGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTGTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAg                     <  2:1265825/140‑1 (MQ=255)
                                                                                                                      ccAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGa                    <  1:6550896/140‑1 (MQ=255)
                                                                                                                         aTGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGaata                 >  1:2483061/1‑140 (MQ=255)
                                                                                                                         aTGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGaata                 >  2:2310788/1‑140 (MQ=255)
                                                                                                                               aCGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGaaa           >  1:6398775/1‑140 (MQ=255)
                                                                                                                               aCGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGaaa           >  2:6539080/1‑140 (MQ=255)
                                                                                                                               aCGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGaaa           >  2:1207391/1‑140 (MQ=255)
                                                                                                                               aCGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGaaa           >  2:266210/1‑140 (MQ=255)
                                                                                                                               aCGCCTTCCTTGATCATCTTTTGCAGGCTTGGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTAGCAGGATCGGCCCAGAAATCAGAATAATGaaa           >  2:3956092/1‑140 (MQ=255)
                                                                                                                                cgacttcattGATCATCTTTTGCAGGCTTTGTTTAGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAg          <  2:1811865/137‑1 (MQ=255)
                                                                                                                                   cttccttGATCATCTTTTGTAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCg       >  1:5584696/1‑140 (MQ=255)
                                                                                                                                     tccttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGc     <  1:4746425/140‑1 (MQ=255)
                                                                                                                                     tccttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGc     >  1:3157871/1‑140 (MQ=255)
                                                                                                                                      ccttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGcc    >  2:6364554/1‑140 (MQ=255)
                                                                                                                                      ccttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGcc    >  2:6135259/1‑140 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  1:4504454/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  1:6861175/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  1:6987962/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  1:2856429/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
AATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAG  >  NZ_CP034943/3319625‑3319900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: