Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,349,666 T→G 5.7% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,349,6660TG 5.7% 1261.2 / 8.5 567intergenic (+1/+26)EO946_RS17305/padChypothetical protein/phenolic acid decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (323/182);  new base G (31/29);  total (354/213)
Fisher's exact test for biased strand distribution p-value = 6.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 573 total aligned reads displayed.

TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349527‑3349804
                                                                                                                                           |                                                                                                                                          
tattTTGTCGGCGGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGTGAAGTGAt                                                                                                                                            >  2:1037614/1‑140 (MQ=255)
   tttGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGACTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATtct                                                                                                                                         <  2:2352402/140‑1 (MQ=255)
          gCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTcc                                                                                                                                  <  2:1868492/140‑1 (MQ=255)
                     tttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATg                                                                                                                       >  2:7002622/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  2:3002576/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  1:5482202/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  1:409737/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCAGGCTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGttt                                                                                                                    >  2:868946/1‑140 (MQ=255)
                          gggACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCAGACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTttat                                                                                                                  <  1:7511767/140‑1 (MQ=255)
                            gACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTttatta                                                                                                                >  1:4709194/1‑140 (MQ=255)
                              cacaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATtata                                                                                                              >  1:7667507/1‑140 (MQ=255)
                                 aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAtt                                                                                                           >  1:6391386/1‑140 (MQ=255)
                                 aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAtt                                                                                                           >  2:4792947/1‑140 (MQ=255)
                                 aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAtt                                                                                                           >  2:6896741/1‑140 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:938521/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:7098685/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:1663315/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTATTTATGTTTATTATAATTTTcc                                                                                                       <  2:6396768/140‑1 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTGTTCCGGGTTTTTATGTTTATTATAATTTTccc                                                                                                      >  1:7300784/1‑140 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCAGACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGGTTCTCTTTTGCCGGTTTTTATGTTTCTTATCATGTTccc                                                                                                      >  1:1161294/1‑140 (MQ=255)
                                      aTTACCAGACTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTGTCCGGGTTTTTATGTTTATTATAATTTTccc                                                                                                      >  1:1939306/1‑140 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTTGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  2:7529248/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCTCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  2:2529936/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:646573/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  2:7313741/140‑1 (MQ=255)
                                        tACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcgc                                                                                                    >  1:6791443/1‑140 (MQ=255)
                                         aCCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcgcg                                                                                                   <  2:4117011/140‑1 (MQ=255)
                                           cAGCCTATGCGCTGGACTGGCATCATTCTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcgcgcg                                                                                                 >  2:7201049/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGCAAGAGACGTGCGTCTCTGTTCCGGGGTTGTATGTTTCTTATAATTTTCCCGCGCGta                                                                                               >  1:3484923/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:2194950/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1546437/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:5938768/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:3173184/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1106908/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:5165730/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:3378983/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:4348466/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1830105/1‑140 (MQ=255)
                                              ccTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGtat                                                                                              <  1:7395198/140‑1 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATc                                                                                           >  2:3988320/1‑140 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATc                                                                                           <  2:7092843/140‑1 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGAGTCTCTTTTCCGGGGTTTTATGTGTATTATAATTTTCCCGCGCGGAGATCg                                                                                          >  1:5785171/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTGCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:3455702/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:2088282/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  2:6728624/1‑140 (MQ=255)
                                                        ggACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTc                                                                                    >  1:3047022/1‑140 (MQ=255)
                                                        ggACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTGTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATCTCGTCTGTc                                                                                    >  2:4051114/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGTTGGTTCCCGCATACGCAGCACGTTGAAAACCCGGGAAAGAGTAGTGAGTCTCTTTTCCGGGTTTTTAGGTTTATTAGAAGTTTCGCGCGCGTCTCTCGTCTGTCa                                                                                   >  2:2065789/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                   >  1:2984745/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTGTATTATAATTTTCCCGCGCGTCTATCGTCTGTCa                                                                                   >  1:4638398/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                   >  2:3722889/1‑140 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:1176664/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:5130503/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  1:2639179/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGATATTATGTTTATTATAATTTTCCCGCGCGGAGATCGTCTGTCAtt                                                                                 <  2:2909751/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  1:2588159/140‑1 (MQ=255)
                                                           cTGGCATCAGACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:2834953/140‑1 (MQ=255)
                                                             ggCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCATCAAAAAGTTAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGTCTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTcc                                                                               <  2:3527467/140‑1 (MQ=255)
                                                             ggCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTcc                                                                               <  2:4348466/140‑1 (MQ=255)
                                                             ggCATCAGACGCTGTGTCGCGGGAATTATGAGGGGTCCCGCATACTCAGCAACAAGACCACCCGGGAAAGAGAAGTGAGTCTCTTGTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTcc                                                                               <  2:6124414/140‑1 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  1:4869851/1‑140 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTATGTCATTGCt                                                                              >  1:506015/1‑140 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  2:5882059/1‑140 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGCTTCTGTTTTCCGGGTGTTTATGGTTAGTATAATTTTCGCGCGCGTATATCGTCTGTCATTCCTTCATa                                                                         >  1:10131/1‑140 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGCTTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATa                                                                         >  2:2656826/1‑140 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATCTCGTCTGTCATTCCTTCATa                                                                         >  2:6471375/1‑140 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  1:3679078/132‑1 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 >  2:131751/1‑132 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTcc                                                                               >  2:4267959/1‑134 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  1:131751/132‑1 (MQ=255)
                                                                     aCTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAg                                                                       <  1:4266405/140‑1 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    <  2:4620305/140‑1 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                   >  1:7001876/1‑140 (MQ=255)
                                                                          ttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGATAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCt                                                                  <  2:5303597/140‑1 (MQ=255)
                                                                             tCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTc                                                               <  1:4008671/140‑1 (MQ=255)
                                                                                  ggAATTATGATGGGTCCCGCATACTCAGCAAAAAGAAAACCCCGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAAt                                                          <  2:4440021/140‑1 (MQ=255)
                                                                                              gTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGtt                                              >  1:4435855/1‑140 (MQ=255)
                                                                                              gTTCCCGCATACGCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTActtct                                                   >  2:5505430/1‑135 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           >  1:3186216/1‑140 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           >  1:5629875/1‑140 (MQ=255)
                                                                                                         cTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTActtct                                                   >  1:2288423/1‑124 (MQ=255)
                                                                                                         cTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCt                                   >  1:3338629/1‑140 (MQ=255)
                                                                                                         cTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGGATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGa                                         <  1:6686458/134‑1 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  1:2865913/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCTCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                              >  2:2189870/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                              >  2:577223/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                              >  1:251573/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTGTTATGTTTATTATAATTTTCCCGCGCGTATATCGTGTGTGATTCCTTCATAAGGAGCGTTCGAAATTACTTCTTCGTGGTCGACTCCCTCAttt                              >  1:3884575/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTGCGGGTTTTTATGTTTCTTATAATTTTCCCGCGCGTATATCGGCTGTCCTTCCTTCATCAGGAGCTTTCGAAATTACTTGTTGGTTGTCGACTCCCTCAttt                              >  2:3894456/1‑140 (MQ=255)
                                                                                                                  aaGAAACCCCGGGAAAGAGAAGTGATTATCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  1:5313626/140‑1 (MQ=255)
                                                                                                                  aaGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  2:4518637/140‑1 (MQ=255)
                                                                                                                  aaGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  2:6006589/140‑1 (MQ=255)
                                                                                                                    gAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTATTCGTTGTCGACTCCCTCATTTTTCAGa                        <  2:3092029/140‑1 (MQ=255)
                                                                                                                      aaaCCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGaaa                      >  1:6106062/1‑140 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         >  1:2772848/1‑140 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  1:1654284/140‑1 (MQ=255)
                                                                                                                                     aaGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCaaa       <  2:2282890/140‑1 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAAtt     >  2:4907512/1‑140 (MQ=255)
                                                                                                                                        tGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTc    <  1:7250043/140‑1 (MQ=255)
                                                                                                                                          aTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAg  >  1:6937337/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349527‑3349804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: