BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,362,393	G→C	8.4%	L99L (CTG→CTC)	EO946_RS17380 →	MazG‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,362,393	0	G	C	8.4%	1250.8 / 16.8	459	L99L (CTG→CTC)	EO946_RS17380	MazG‑like family protein
Reads supporting (aligned to +/- strand): ref base G (271/131); new base C (2/48); total (275/184)
Fisher's exact test for biased strand distribution p-value = 3.57e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

ATGAGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCT  >  NZ_CP034943/3362254‑3362532

atgaGAAAGAATCGACACGAGCTGAGCAAAAACAATAATTGATTGAAGAAATGGGGGAGGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGGCTCTTTAGAAGTCGTGATGAAAGCCCACCAAGAAAAACTg                                                                                                                                             >  1:3658053/1‑140 (MQ=255)

atgaGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTg                                                                                                                                             >  1:1819346/1‑140 (MQ=255)

atgaGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATGGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTg                                                                                                                                             >  1:1476108/1‑140 (MQ=255)

atgaGAAAGAATCGACACGAGCTGAGCAAAAACAAGAAGTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATGCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTg                                                                                                                                             >  1:5698621/1‑140 (MQ=255)

    gaaagaaTCTACACTAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCa                                                                                                                                         <  2:5009107/140‑1 (MQ=255)

    gaaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCa                                                                                                                                         <  1:6871489/140‑1 (MQ=255)

    gaaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCa                                                                                                                                         <  1:3949165/140‑1 (MQ=255)

    gaaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCa                                                                                                                                         <  1:3173971/140‑1 (MQ=255)

     aaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                        >  1:470499/1‑140 (MQ=255)

          aTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGtt                                                                                                                                   >  1:2443340/1‑140 (MQ=255)

                acGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgc                                                                                                                             <  1:2742760/140‑1 (MQ=255)

                acGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgc                                                                                                                             <  1:2028443/140‑1 (MQ=255)

                acGAGCTGAGCAAAAACAAGAATGGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgc                                                                                                                             <  2:2874842/140‑1 (MQ=255)

                  gAGCTGATCAAAAACAAGACTTGATTGAAGAAATGGGGTATTTCATCTGATATATAGCCTTTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACTTGATGATAGCCCACCAAGACAAACTGACCAAACGTTTTGAgctt                                                                                                                           <  2:1906009/140‑3 (MQ=255)

                  gAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCACACGTTTTGAgcat                                                                                                                           <  1:4190024/140‑1 (MQ=255)

                  gAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgcat                                                                                                                           <  1:1945434/140‑1 (MQ=255)

                   aGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgcatg                                                                                                                          >  1:1375283/1‑140 (MQ=255)

                    gCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgcatgc                                                                                                                         <  1:551111/140‑1 (MQ=255)

                    gCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgcatgc                                                                                                                         <  1:301208/140‑1 (MQ=255)

                       gAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATa                                                                                                                      >  1:5081822/1‑140 (MQ=255)

                        aGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATaa                                                                                                                     >  1:4473224/1‑140 (MQ=255)

                             aaaCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATTTACGGAGTCTCTTTAGAATACGTGATGAAAGCCCACCAAGAAAAACTGACCAAAAGTTTTGAGCATGCATAAAAAAg                                                                                                                >  2:5418992/1‑140 (MQ=255)

                             aaaCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAg                                                                                                                >  1:4329929/1‑140 (MQ=255)

                                  aGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAAAGTTTTGAGCATGCATAAAAAAGCggac                                                                                                           >  2:3815022/1‑138 (MQ=255)

                                             aagaaATGGGGTATGTCATCGGAAATATCGCAATTCTTGCTCATTTGAACTGAGTCTATTTAGAAGACTGTATGAAAGCCCACCAAGAAAAACTGCCCAAACTTTTTGTCCTTGCATAAAAAAGCGGCCGTAATGGCCGc                                                                                                <  2:2285403/140‑1 (MQ=255)

                                             aagaaATGGGGGATGTCATCGTAAATATCGCCATTCTGGCTGATATGTCCGTCGTCGCTTTAGAAGACGTGATGAACGCCCACCAAGAAAAACTCACCACACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGc                                                                                                <  2:4464456/140‑1 (MQ=255)

                                                   tGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCtttttt                                                                                          >  1:2940546/1‑140 (MQ=255)

                                                    gggggATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAACGCCCACAAACAAAACCTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCttttttt                                                                                         <  2:5918990/140‑1 (MQ=255)

                                                         cggTCACCGGAAATATCCCCATTCTTGCTGAAATGGACGGAGGCTCTTTAGAATACGTGATGAACGCCCACCACTAAAAACTGCCCAACCGTTTTTCGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCt                                                                                    <  2:1296832/138‑1 (MQ=255)

                                                         aTGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCt                                                                                    >  2:6525138/1‑140 (MQ=255)

                                                          tGTCATCGGAAATATCGCCATTCTTTCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATCGCCGCTTTTTGTAACCtg                                                                                   >  1:2676961/1‑140 (MQ=255)

                                                          tGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCtg                                                                                   >  1:5614078/1‑140 (MQ=255)

                                                           gTCATCGTAAATATCGCAATTCTTGCTGATCTGTAGGGAGTCTTTTTATAACACGTGATGAAACCCCACAAATAAAAACTGACCAAACGTTTTGAGCAGGCATAAAAAAGCGGCCGTAAGGGCCGCTTTTTTTAACCtgt                                                                                  <  2:2623176/140‑1 (MQ=255)

                                                           gTCATCGGAAATATCGCCATTCTTGCTTATATGTACGGATTCTTTTTATAAGACGTGATTAAAGCCAACAAACAAAAACTTACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCtgt                                                                                  <  2:470499/140‑1 (MQ=255)

                                                                 ggAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGAtttg                                                                            >  1:533036/1‑139 (MQ=255)

                                                                  gaataatCCCCATTCTTGCTCATATGTACGGAGTCTCTTTAGATGACGTTATGAAAGCCCACAAAGAAAACCTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTtca                                                                           <  2:3162677/135‑1 (MQ=255)

                                                                  gAAATAACGCAATTCTGGCTGATATGTACGGATTCTCTTTACACGACGTGATGAACGCCAACCAACAAAAACTCACCAACCGTTTTGAGCATCAAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTtca                                                                           <  1:958322/140‑1 (MQ=255)

                                                                      tatCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAATACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGACGTAATGGCCGCTTTTTTTAAACTGTGATTTcatcat                                                                       >  2:4273467/1‑140 (MQ=255)

                                                                       atCGCCATTCTTGCTTATATGTACGGTGTCTCTTTATAACACGTTATGAAAGCCCACCAAGAAAACCTGACCAAACGTTTTGTGCATGCATAAAACCGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATg                                                                      <  2:3125992/140‑1 (MQ=255)

                                                                        tCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATgg                                                                     >  2:5696768/1‑140 (MQ=255)

                                                                           ccATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGa                                                                  >  2:398138/1‑140 (MQ=255)

                                                                           ccATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGa                                                                  >  2:5885736/1‑140 (MQ=255)

                                                                             aTTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATAATGGTGATc                                                                >  2:6835851/1‑140 (MQ=255)

                                                                             aTTCTTGCTGATAGGTACGGAGTCTCTTTAGAACACGTGATGAAATCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAta                                                                >  2:935383/1‑139 (MQ=255)

                                                                              ttCTTGCTGATATGTCCGGAGTCTCTTTAGAAGACGTGAGGAAAGCCCACCAAGAAAAACTGAGCAATCGTTTTGAGCATGCATAAATAAGGGGCCGTACTGGCCGCGTGTTTTACCGTGTGATATCATCATggggctcc                                                               >  2:6217626/1‑134 (MQ=255)

                                                                                 ttGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTTAGTTCAACATGGTGATaaaat                                                            >  2:3577468/1‑139 (MQ=255)

                                                                                      tattggTACGGAGTCTATTTAGAAGACGTGATGAAAGCCCACCAATAAAACCTGACCAAACTTTTTGAGCTTTAAAAAACACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtg                                                       <  1:6466581/135‑1 (MQ=255)

                                                                                      gATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtg                                                       >  2:4074368/1‑140 (MQ=255)

                                                                                         atGTACGGCGTCTCTTTAGAAGATGTGAAGAACGCCCACCACCAAAAACTCACCCACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCAGCTTTTTTTAACCTGTGATTTCCTGATGGTGATCAAAGAAAtgctg                                                    <  2:4780834/140‑1 (MQ=255)

                                                                                         atGTACGGAGTCTCTTTAGACGACGTGATGAAAGCCCACCAACAAACACTGACCAAACGTTTTGAGCATGCATACAAAAGCGGCCGGAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                    <  2:7295231/140‑1 (MQ=255)

                                                                                         atGTACGGAGTCTCTTTAGAATACGTGATTAAAGCCCACCAATAAAAACTGACCAAACTATTTTAGCATGCATAAAAAAGCGGCCGTAATTCCCGCTTTTTTTAACCTGTTATTACATCATGGTGATCAAAGAAAtgcag                                                    >  1:7133013/1‑138 (MQ=255)

                                                                                         atGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                    <  2:3045471/140‑1 (MQ=255)

                                                                                         atGTACGGAGTCTCTTTACAAGACGTGATGAAACCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                    <  2:6920288/140‑1 (MQ=255)

                                                                                             aCGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGAATTCATCATGGTGATCAAAGAAATGCTGCttt                                                >  2:3904836/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                            >  2:1630352/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                            >  2:2085512/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                            >  1:816581/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                            >  2:6335620/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAACTGTTATTTAATAATTGTGATCAAAAAAATGCTGCTTTACTa                                            >  2:6052290/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                          >  2:2603503/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCGAtt                                          >  1:6950877/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGACGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAATGAAATTCTGCTTTCCTAtt                                          >  2:7517878/1‑140 (MQ=255)

                                                                                                    ctctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTACTAttt                                         >  1:668090/1‑140 (MQ=255)

                                                                                                     tctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATCAAAGAAATGCTGCTTTACTAtttt                                        >  2:2731149/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAttttt                                       >  2:4126679/1‑139 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                       >  1:6596813/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAGTTtg                                       >  1:1562975/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTGTACTATTTtg                                       >  2:7181084/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGGAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                       <  1:5547819/140‑1 (MQ=255)

                                                                                                         tAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGa                                    <  2:2443340/140‑1 (MQ=255)

                                                                                                           gaagaCGTTATGAAAGCCCACCACGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  2:5244436/140‑1 (MQ=255)

                                                                                                           gaagaCGTGATGAACGCCCACCAAGAAAACCTGCCCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  2:3718716/140‑1 (MQ=255)

                                                                                                           caagaCTTTATGAACGCCCACAAATAAAAACTCACCAAACGTTTTGAGAATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  1:3384015/139‑1 (MQ=255)

                                                                                                           caagaCGTGATGAAAGCCCACAAAGAAAAACGGACCAACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  1:5170424/139‑1 (MQ=255)

                                                                                                               aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAATAAATGCTGCTTTCCTATTTTGTGAAATatg                              >  1:1211782/1‑138 (MQ=255)

                                                                                                               aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAgg                              >  1:1254742/1‑140 (MQ=255)

                                                                                                               aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAGTTTGTGAAATAgg                              >  2:6734628/1‑140 (MQ=255)

                                                                                                                ggTGATGACAGCCCACCAAGAAAAACTCACCAAACTTTTTTCTAAGGCATACCAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:220984/139‑1 (MQ=255)

                                                                                                                cttgatcaAACCCCACCAATAAAACCTCCCCAACCTTTTTGAGCATCAATAAAAACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:6280169/138‑1 (MQ=255)

                                                                                                                cGTTATGACCGCCCCCCAAGAAAAACTGACCAAACGTTTTGAGCATCCATAAAACAGCGGCCGTAATGGCCGCTTTTTTTAACCTGGGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:4899738/140‑1 (MQ=255)

                                                                                                                cGTTATGAAAGCCCACCAACAAAACCTGACCAAACTTTTTGACCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTACCCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:3269000/140‑1 (MQ=255)

                                                                                                                cGTGATTAAAGCCCACAAAGAAAAACTGACCAAACGTTTTGAGCTTGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:7651852/140‑1 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAAAACTGACCAACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:7536168/140‑1 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             >  1:7248805/1‑140 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:5081822/140‑1 (MQ=255)

                                                                                                                cGTCATTAACGCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:5268496/140‑1 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCCTGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:1393438/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTGCTATTTTGTGAAATAGGcc                            >  1:2605463/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:1045456/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  1:1694502/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:1502371/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:1822258/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGATTTCCTATTTTGTGAAATAGGcc                            >  1:7403166/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGGGATCAAAGAAATGCTGCTTTCCTGTTTTGTGGAATAGGcc                            >  2:3383076/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAACGGTGATTTCATGATGGTGTTCAAAGAAATGCTGCTTTGCTATTTTGTGAAAAAGGcc                            >  1:1069097/1‑140 (MQ=255)

                                                                                                                         aGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAACTGTGATTTCATCATGGTGATAAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTAt                    >  1:4887691/1‑140 (MQ=255)

                                                                                                                           cccACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCCTGGTGATCAAAGAAATGCTGCTTTCCTCTGTTGTGAACTGGGCCGGTGTTATgc                  >  2:4629132/1‑140 (MQ=255)

                                                                                                                              accaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAGCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagc               >  2:3662086/1‑140 (MQ=255)

                                                                                                                              accaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagc               <  1:5918785/140‑1 (MQ=255)

                                                                                                                              accaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagc               >  2:554234/1‑140 (MQ=255)

ATGAGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCT  >  NZ_CP034943/3362254‑3362532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`