BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	314,425	T→G	5.4%	L83R (CTT→CGT)	niaP →	niacin permease NiaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	314,425	0	T	G	5.4%	1162.5 / 4.6	431	L83R (CTT→CGT)	niaP	niacin permease NiaP
Reads supporting (aligned to +/- strand): ref base T (172/210); new base G (42/3); total (217/214)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

CTCCACGTAGAGTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCGGTTGT  >  NZ_CP034943/314286‑314563

      gTAGAGTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATg                                                                                                                                      <  1:4057491/140‑1 (MQ=255)

        agagTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCt                                                                                                                                    <  2:3909373/140‑1 (MQ=255)

          agTGGACTCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCttt                                                                                                                                  >  2:5243991/1‑140 (MQ=255)

          agTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCGTTTTATGCttt                                                                                                                                  >  2:5636531/1‑140 (MQ=255)

          agTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCttt                                                                                                                                  >  2:1649223/1‑140 (MQ=255)

          agTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCttt                                                                                                                                  >  2:6778707/1‑140 (MQ=255)

          agTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTGTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTGTTATGCttt                                                                                                                                  >  1:585107/1‑140 (MQ=255)

          agTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGGTGTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCGTTTATGCttt                                                                                                                                  >  1:7618985/1‑140 (MQ=255)

           gTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCtttt                                                                                                                                 >  2:5965909/1‑140 (MQ=255)

             ggAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGTTCGGATCGGCCGCAAAAAAGTGTTTATCATTAACCGTTTATGCttttac                                                                                                                               >  1:100562/1‑138 (MQ=255)

             ggAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATAATTAACCTTTTATGATTTTcc                                                                                                                               >  2:4010095/1‑140 (MQ=255)

             ggAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGGTTATCATTACCCTGTTATGCTTTTcc                                                                                                                               >  2:5145262/1‑140 (MQ=255)

               aaTCTGTCGCCCGAAGAAATGAAATGTATCGGAAGCTTCAATTCCATCGGCATGGCTGCGGGGGCGTTTGTGGTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGGTTATCAATACACTGTGATGCTTGTACAt                                                                                                                             >  2:4253847/1‑140 (MQ=255)

               aaTCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTGTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCGTTTATGCTTTTACAt                                                                                                                             >  1:1969268/1‑140 (MQ=255)

                 tCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTGTTTGTTTGGTCTGCTGGCTGATCGGCTCGGCCGCAAAAAAGTGTTTATCCTTACCCGTTTTTTCTTTTCCATCg                                                                                                                           >  1:7044222/1‑140 (MQ=255)

                    ggcGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGGTTTTGGTTGGTCTGCTGGCTGTTGGGATCGGCCGCAAAAAAGTGTGTATCATTACCCTTTTCTGCTTTTCCATCGGac                                                                                                                        >  2:446935/3‑139 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTGTGCTGGGTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCGTTTATTCTTTTGCATCGGaa                                                                                                                        >  1:4874435/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAATTGTTTATCATTACCCGTTTATGCTTTTACATCGGaa                                                                                                                        >  1:14336/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACGCTTTTATGCTTTTCCATCGGaa                                                                                                                        >  2:3971644/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGaa                                                                                                                        >  2:7379643/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGaa                                                                                                                        >  2:4781985/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGaa                                                                                                                        >  2:6929974/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCGTTTATGCTTTTCCATCGGaa                                                                                                                        >  2:2981976/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATAAGTACCCGTTTATGCTTTTCCATCGGaa                                                                                                                        >  1:2591739/1‑140 (MQ=255)

                    gTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTGTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGac                                                                                                                        >  2:6461867/1‑139 (MQ=255)

                     tCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATGACCCTTTTATGCTTTTCCATCGGAAg                                                                                                                       <  1:6551490/140‑1 (MQ=255)

                      cGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTGTTTTTTTTGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATAAATACCCTTTTTTGCTTTTCCATCGGAAGc                                                                                                                      >  1:1518871/1‑140 (MQ=255)

                              gaaatgaaatGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTc                                                                                                              <  1:4637230/140‑1 (MQ=255)

                              gaaatgaaatGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTc                                                                                                              <  1:5540106/140‑1 (MQ=255)

                              gaaatgaaatGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTc                                                                                                              <  2:3617353/140‑1 (MQ=255)

                                 atgaaatgGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTGTTTGTTTGGTGTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTTTCATTACCCATTTATGCTTTTACCTCGGAATCCGCAATTcccc                                                                                                           >  1:6848807/1‑138 (MQ=255)

                                          ctCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTGTCCATCGGAAGCGGCATTTCCGCTTTTGTGAc                                                                                                  <  1:7406715/139‑1 (MQ=255)

                                          aTCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGAc                                                                                                  <  2:7547728/140‑1 (MQ=255)

                                           tCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACg                                                                                                 <  2:491843/140‑1 (MQ=255)

                                           tCGGAAGCGTCAAGTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGTTCTGCTGGCTGATCGGATCGGCCGCAAACAAGGGTTTATCATTACCCTGTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACg                                                                                                 <  2:4388195/140‑1 (MQ=255)

                                                    tCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGGTGATCGGTTCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTGCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCg                                                                                        >  1:6119711/1‑140 (MQ=255)

                                                      aaTTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGc                                                                                      <  2:1820528/140‑1 (MQ=255)

                                                       aTTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCa                                                                                     <  2:3238791/140‑1 (MQ=255)

                                                        ttCCATCGGCATGGCTGCGGGGGCGTTTTTGGTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTGTGTTTACCCTTTTATGCTTTTCCATCGGAAGCGGGATTTCCGCTTTTGTGACGAGCTTATCGGCAt                                                                                    >  2:176854/1‑140 (MQ=255)

                                                         tCCATCGGCATGGCTGCGGGGGCGTTGGTGTGTGGTCTGCGGGCTGATCGGATCGGCCGCAAAATAGGGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTCTCGGAAtt                                                                                   >  2:6225929/1‑140 (MQ=255)

                                                           cATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTcc                                                                                 <  2:506319/140‑1 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTTCCCTGTTATGGTTTTCCATCGGCAGCGGCATTTCGGGTTTTTTGACGAGCTTTTCGGCAGTCCTc                                                                               >  2:2447723/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCGTATCGGCATTCCTc                                                                               >  2:6550267/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTACATCGGAAGCGGCATTTACGCTTTTGTGACGAGCTTATCGGCATTCCTc                                                                               >  1:2234645/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTGTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTTTGTCGAGCTTATCGGCATTCGTc                                                                               >  2:4680333/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCGTTTATGCTTTTCCATCGGAAGCGGCCTTTCCGCGTTTGTGACGAGCTTATCGGCATTCCTc                                                                               >  2:1258096/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCGTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGATCTTATCGGCATTCCTc                                                                               >  2:7204897/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGAGCGGCCCCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGTCGAGCTTATCGGCATTCCTc                                                                               >  1:4823379/1‑140 (MQ=255)

                                                             tCGGCATGGCTGCGGGGGCGTTTGTGTGTTGTCTGCTGGCTGATCGGATCGGCCGCAAAAAACTGTGTATCATTCCGCGTTTATTCTTTTGCAAGGGAAGCGGCATGTGCGGTTTTCTGACTAGCTTATCGGCCTTCCTc                                                                               >  2:5875245/1‑140 (MQ=255)

                                                             tCGGCAGGGCTGCGGGGGCGTGTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTAATCATTACCCTTTTATGGTTTTCCAACCGAAGCGGCATTACCGCTTTTGTGACGAGCGTATCGGCCTTCCTc                                                                               >  1:5689805/1‑140 (MQ=255)

                                                                 cATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTAACCTTTTATTCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATAGGCATTACGCAttt                                                                           >  1:3263307/1‑140 (MQ=255)

                                                                   tGGCTGCGGGGGCGTGTTTGTTTGGTCTGCTGGCTGATCGGGTCGGCCCCAAAAAAGGGTTTATCAGTACCCGTTTATTCTTTTCCATCGGAAGCCGCAATTACGCTTTTGTGACGAGCTTATAGGCATTACTCAGTTTg                                                                         >  1:4271979/1‑140 (MQ=255)

                                                                      cTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGt                                                                      <  1:6059840/140‑1 (MQ=255)

                                                                         cGGGTGCGTTTTTGTTTGGTTTGCTTGCTGATCGAATCGGCCGCAAAAAAGTGTTCATCATCACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTTCTAATCCTTCGTTTc                                                                   >  1:6685553/1‑140 (MQ=255)

                                                                         cGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTAACCGTTTATTCTTTTCCATCGGAAGCGGCAATTCCGCTTTTTTTACGAGCGTATCGGCAATCCTCATTTTTGGttta                                                                   >  1:6210102/1‑139 (MQ=255)

                                                                         cGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATAGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATTCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGATCTTATCGGCATTCCTCATTTTGCGTTTc                                                                   >  1:5374398/1‑140 (MQ=255)

                                                                         cGGGGGCGTTTGTGTTTGGTCAGCTGGCTTATCGGATCGGCCGCAAAAAATTGTTTATCATTACCCTTTTATGCTTTTCCATACGAAGAGGCATTTACGCATTTGTGACGAGCTTATCGGCATTACACATGATTCGTTTc                                                                   >  2:3296771/1‑140 (MQ=255)

                                                                              gCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCAt                                                              <  2:3637272/140‑1 (MQ=255)

                                                                              gCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCAt                                                              <  2:1166794/140‑1 (MQ=255)

                                                                              gCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTCTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCAt                                                              <  2:2307095/140‑1 (MQ=255)

                                                                              gCGTTTTTGTGTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCAt                                                              <  2:7268916/140‑1 (MQ=255)

                                                                               cGTGTTTGTTTTGTCTGCTGGCTGATCGGATCGGCCGCAAAAAATTGTTTATCATTACCCGTTTATTCTTTTACATCGGAAGCGGCATTTACGCTTTTTTGACGAGCTTTTCGGCATTCCTCATTTTGCGTTTCGTCATc                                                             >  1:3642201/1‑140 (MQ=255)

                                                                                 tttttGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCgg                                                           >  2:711052/1‑140 (MQ=255)

                                                                                   tttgtttgGTCTTCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCAATTTGCGTTTCGTCATCGGaa                                                         <  2:5347928/140‑1 (MQ=255)

                                                                                   tttgtttgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGaa                                                         <  2:4529627/140‑1 (MQ=255)

                                                                                   tttgtttgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGGTCATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGaa                                                         <  2:4810627/140‑1 (MQ=255)

                                                                                   tttgtttgGTCTGCTGGCTGAGCGGATCGGCCGCAAAAAAGTTTTGATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCAGTCCTCATTTTGCGTTTCGTCATCGGaa                                                         <  2:4318387/140‑1 (MQ=255)

                                                                                   tttgtgtgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGaa                                                         <  2:2656645/140‑1 (MQ=255)

                                                                                    ttgtttgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAAt                                                        <  1:2781323/140‑1 (MQ=255)

                                                                                    ttgtttgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAAt                                                        <  1:1647581/140‑1 (MQ=255)

                                                                                    ttgtttgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAAt                                                        <  1:4411438/140‑1 (MQ=255)

                                                                                    ttgtttgGTCTGATGGCTGATCGGATCGGCCGCAAACAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAAt                                                        <  1:7207001/140‑1 (MQ=255)

                                                                                     tgtttgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATg                                                       <  1:2382265/140‑1 (MQ=255)

                                                                                         tgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGc                                                   <  2:3096976/140‑1 (MQ=255)

                                                                                         tgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGc                                                   <  2:3444649/140‑1 (MQ=255)

                                                                                         tgGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGc                                                   <  2:2396957/140‑1 (MQ=255)

                                                                                          ggTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCCTCGGAAGCGGCATTTCCGCTTTGGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCt                                                  <  1:2059571/140‑1 (MQ=255)

                                                                                          ggTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCt                                                  <  1:7258185/140‑1 (MQ=255)

                                                                                             ctgctgGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAgg                                               <  1:3461932/140‑1 (MQ=255)

                                                                                                  ggctgATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGgag                                          <  2:1998584/140‑1 (MQ=255)

                                                                                                      gatcggatcgGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCt                                                  >  2:4139752/1‑128 (MQ=255)

                                                                                                      gatcggatcgGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCt                                      >  1:2448813/1‑140 (MQ=255)

                                                                                                      gatcggatcgGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCGCAGTTTGCGTTTCGTCATCTGAATGGGGCTAGGCGGAGAGCt                                      >  1:1247914/1‑140 (MQ=255)

                                                                                                            atcggCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGGTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGt                                <  2:862018/140‑1 (MQ=255)

                                                                                                             tcggCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTAt                                                                                          >  2:6640055/1‑81 (MQ=255)

                                                                                                             tcggCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTAt                                                                                          <  1:6640055/81‑1 (MQ=255)

                                                                                                             tcggCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGtt                               >  2:707415/1‑140 (MQ=255)

                                                                                                             tcggCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTGTTGTGACGAGCTTATCGGCATTCCTCAGTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGtt                               >  2:1031380/1‑140 (MQ=255)

                                                                                                                      aaaaaGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAAcc                      >  1:854980/1‑140 (MQ=255)

                                                                                                                              tttATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTc              >  2:1549617/1‑140 (MQ=255)

                                                                                                                              tttATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTc              >  2:6184366/1‑140 (MQ=255)

                                                                                                                               ttATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCtt                                                                                            <  1:4521269/61‑1 (MQ=255)

                                                                                                                                   cATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAg         <  1:1097229/140‑1 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCTCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGCGAGCTTCCGGTTGATTCAACCCTCGTTTCGGAAGCgg      >  1:6352982/1‑140 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCgg      >  2:2111359/1‑140 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCgg      >  1:914771/1‑140 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCgg      >  1:3924886/1‑140 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCgg      >  1:3103352/1‑140 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCAGTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCgg      >  1:5213181/1‑140 (MQ=255)

                                                                                                                                      tACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCAGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCTGTTGCTTCAACCCTCGTTTCGGAAGCgg      >  1:469232/1‑140 (MQ=255)

CTCCACGTAGAGTGGAATCTGTCGCCCGAAGAAATGAAATGGATCGGAAGCGTCAATTCCATCGGCATGGCTGCGGGGGCGTTTTTGTTTGGTCTGCTGGCTGATCGGATCGGCCGCAAAAAAGTGTTTATCATTACCCTTTTATGCTTTTCCATCGGAAGCGGCATTTCCGCTTTTGTGACGAGCTTATCGGCATTCCTCATTTTGCGTTTCGTCATCGGAATGGGGCTAGGCGGAGAGCTTCCGGTTGCTTCAACCCTCGTTTCGGAAGCGGTTGT  >  NZ_CP034943/314286‑314563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`