BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,394,360	T→G	11.3%	K180N (AAA→AAC)	ppaX ←	pyrophosphatase PpaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,394,360	0	T	G	11.3%	1105.2 / 26.0	481	K180N (AAA→AAC)	ppaX	pyrophosphatase PpaX
Reads supporting (aligned to +/- strand): ref base T (177/226); new base G (76/2); total (253/228)
Fisher's exact test for biased strand distribution p-value = 1.21e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 524 total aligned reads displayed.

CGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAA  >  NZ_CP034943/3394222‑3394499

cGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGttt                                                                                                                                            <  2:3108853/140‑1 (MQ=255)

cGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGttt                                                                                                                                            >  2:222984/1‑140 (MQ=255)

cGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGttt                                                                                                                                            >  2:6923936/1‑140 (MQ=255)

        gATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCg                                                                                                                                    <  2:1891277/140‑1 (MQ=255)

                 ttCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  2:4743383/140‑1 (MQ=255)

                 ttCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  2:582823/140‑1 (MQ=255)

                 ttCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  2:2294138/140‑1 (MQ=255)

                     cacaGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTg                                                                                                                       <  1:5094331/140‑1 (MQ=255)

                      acaGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTATCAAGAATATAATCAGGATCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGAGTCGTTTTTCCCTGc                                                                                                                      >  2:3987394/1‑140 (MQ=255)

                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTACGGCGTTTTTCCCTGCCa                                                                                                                    >  2:2641619/1‑140 (MQ=255)

                         gATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAg                                                                                                                   <  2:3041832/140‑1 (MQ=255)

                         gATTACTTCACTCCAACGATGTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAg                                                                                                                   <  2:7227405/140‑1 (MQ=255)

                             aCTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGcaca                                                                                                               <  2:3793258/140‑1 (MQ=255)

                                 cACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGGTTTTCCCTGCCAGCACATCgg                                                                                                           >  1:6446509/1‑139 (MQ=255)

                                  aCTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  1:2958940/140‑1 (MQ=255)

                                  aCTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  2:1756128/140‑1 (MQ=255)

                                   cTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTgg                                                                                                         <  2:7416536/140‑1 (MQ=255)

                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTTAGGGGTTTTTGACTGGCAGCAAATAGTGGt                                                                                                        >  1:4520010/1‑140 (MQ=255)

                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTACCTGCCCGCACATCGTGGt                                                                                                        >  1:1908097/1‑140 (MQ=255)

                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGGGTTTTTGCCGGCCCGCACAACGTTGt                                                                                                        >  1:4816395/1‑140 (MQ=255)

                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTCCCAGCCAGCACAACGGGGt                                                                                                        >  1:1550103/1‑140 (MQ=255)

                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTACGGCGGTTTTTCCGGCCAGCAAAACGTGGt                                                                                                        >  1:1118216/1‑140 (MQ=255)

                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGGGGCGGTTTTCACTGCCAGcccaaaggggt                                                                                                        >  1:6671344/1‑133 (MQ=255)

                                       aacgcattGTAATAGATCACTCATTTTCTCAAGCATATAATCCGGCTCATGCTTTGCCAGTGGTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAAt                                                                                                     <  1:1491682/134‑1 (MQ=255)

                                            tttGTATTAGATCACTCATTTTCTCATGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTAtct                                                                                                <  2:3193004/140‑1 (MQ=255)

                                              tGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  2:5060340/140‑1 (MQ=255)

                                              tGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  1:3235636/140‑1 (MQ=255)

                                              tGTAATAGATAACTCATTTTCTCAAGGATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCAGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  1:5743625/140‑1 (MQ=255)

                                                tAATAGATCACTCATTGTCTCAAGCATATAATCAGGCTGATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGa                                                                                            <  1:458269/140‑1 (MQ=255)

                                                 aaTAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGAc                                                                                           <  2:2447708/140‑1 (MQ=255)

                                                     gATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTACCAGCCCGCACAACGTGGTACTTATATCCGAcccaa                                                                                       >  1:5889803/1‑137 (MQ=255)

                                                     gATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATa                                                                                       <  1:2782793/140‑1 (MQ=255)

                                                     gATCACTCATTGTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATa                                                                                       <  1:6912966/140‑1 (MQ=255)

                                                      aTCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTAACAGCCAGCACAACCTGGTAATTATATACGACCaaaa                                                                                      >  1:1010076/1‑140 (MQ=255)

                                                        cACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTGCCGGGCCGGCCATCGGGGTATTTATATCCCCCCCTAATg                                                                                    >  1:3139591/1‑140 (MQ=255)

                                                          cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTTCGGCGGTTTTGCCTGCCAGCACAACGTGGTAAATATCTCCGACCaaaaatgc                                                                                  >  1:941679/1‑136 (MQ=255)

                                                          cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGc                                                                                  >  2:5396174/1‑140 (MQ=255)

                                                          cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTCCCAGCCAGCACCACGTTGTAATTATCAGCGACCATAATTGc                                                                                  >  2:4623201/1‑140 (MQ=255)

                                                          cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTACCTTCCAGCACAACCGGGTAATTATCTCCGACCAAAAtttc                                                                                  >  2:5286647/1‑137 (MQ=255)

                                                                     aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                       <  1:2095416/140‑1 (MQ=255)

                                                                     aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                       <  1:1704787/140‑1 (MQ=255)

                                                                     aaGCATATAATCAGGCTCATGCTTTGACAGTGTTTCCGGCCCTTTAATCGTCGATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                       <  2:2025462/140‑1 (MQ=255)

                                                                       gCATATAATCAGGCTCCTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                     <  1:7492365/140‑1 (MQ=255)

                                                                       gCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                     <  1:2481542/140‑1 (MQ=255)

                                                                       gCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTGAATCGTCCATGCGACTCCGGCCGTTTTGGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                     <  2:6020075/140‑1 (MQ=255)

                                                                           ataATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTACCTGGCACCACATCGTGGTAATTATATCCCCCCATAATGGCTTCCGCCGGCTCGCGTc                                                                 >  2:6127888/1‑140 (MQ=255)

                                                                                  ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTCCCTGCCAGCACATAGTGGTAATTATATCCGACCATAATGGGTTGCGCCGGCGCGCTTCCTAGCTg                                                          >  2:818491/1‑140 (MQ=255)

                                                                                  ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTACGGCGTTTTTACCGGCCAGCCCAACGTGGTAATTATCTCCCACCAAAATTGCGTCCGCCGGCGCCCCTCCTAGcag                                                          >  2:942268/1‑138 (MQ=255)

                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTTCGGGGTTTTTACCTGGCCGGACAGCGTGGTAATTATATCCGACCATAATGTGTTCCGCCGGCAGGCGTCCTAGCGGt                                                         >  1:3959690/1‑140 (MQ=255)

                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCg                                                                                                                                    <  1:5271825/65‑1 (MQ=255)

                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTCCGGGGGTTTTACCGGCCCGCCCCTCGTGGGAATTTTCTCCGACCATAATTGCTTACGCCGGCTCGCGTCCTATCTGt                                                         >  1:5528257/1‑140 (MQ=255)

                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGGTTGTTGCGGGGTTTTTGGCGGCCCGCCCAGCGGGGTTAATATCTGCGCCCCAAATAGCTTGCCCCCGCGCGCGTccgaacggt                                                         >  1:3071408/1‑133 (MQ=255)

                                                                                    cTCATGCTTTGCCCGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGGTTTTCCCAGCCCGCCCATCGTGGTAATTATATACGAACATAAATGCTTACCCCGGCAAGCTTCCAAGCTGtt                                                        >  2:3372637/1‑140 (MQ=255)

                                                                                      cATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTc                                                      <  1:3421234/140‑1 (MQ=255)

                                                                                       aTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTACCTTCCACCCCATCGTGGTAATTTTGTCCGACCAAAATTGGTTCCGCCGGCGCGCGTGCTAGCTGtttaa                                                     >  1:7634706/1‑138 (MQ=255)

                                                                                         gCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAgc                                                   <  1:7376110/140‑1 (MQ=255)

                                                                                             tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTCCCAGCCCCCACATCCTGGTTATTATATACGACCAAAAATGGTTACCCCGGCGACCGTCCGAGCAGTTTAAGCGCTa                                               >  1:3615311/1‑140 (MQ=255)

                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACCTCGTGGTAATTATCACCGACCATAATTGCTTCCGCCGGCTCGCTTCCTAGCTGGTTCAGCGCTAAAAGca                                         >  2:7647190/1‑140 (MQ=255)

                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGGTGCGGGGTTTTTACCTGCCCGCCCCACGGGGTAATTATCTACCACCCTAAAGGCGTCCCGCGGCGCGCGTACTAGCTGTTTGCGCGCTAAAATca                                         >  1:7159456/1‑140 (MQ=255)

                                                                                                    gtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGcac                                        <  2:3878940/140‑1 (MQ=255)

                                                                                                     tttCCGGCCCTTTCATCGTCCATGCGACTCCGGACGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGcaca                                       <  2:7374609/140‑1 (MQ=255)

                                                                                                     tttCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGcaca                                       <  2:7077519/140‑1 (MQ=255)

                                                                                                     gttCCGGCCCTTTCATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGcaca                                       <  2:1224087/139‑1 (MQ=255)

                                                                                                         cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGTTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                   <  1:1366807/140‑1 (MQ=255)

                                                                                                         cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                   <  1:2376832/140‑1 (MQ=255)

                                                                                                         cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                   <  1:837369/140‑1 (MQ=255)

                                                                                                         cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                   <  1:3755846/140‑1 (MQ=255)

                                                                                                         acagccTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                   <  1:7016597/136‑1 (MQ=255)

                                                                                                          ggCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTACCTGCCAGCACATCGTGGTAAATATCTACGACCATAATTGCTTACGCCGGCTCGCGTCCAAGCAGTTTCAGCGCTAAAAGCACACGcgc                                  >  2:7046993/1‑138 (MQ=255)

                                                                                                          ggCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTACCTGCCAGCACAGCGTGGGAATTATATACGACCATAAAGGCTTCCGCCCGCTCGCTTCCTAGCTGTGTCAGCGCTAAAAGCACACGCTc                                  >  2:5126318/1‑140 (MQ=255)

                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTGCCGGCCAGCACCACGTGGTAATTATCTCCGACCATAATTGCGTCCGCCGGCTCGCGTCCTAGCTGTTTCAGCCCTAAAAGCACAGGCGCGGGag                             >  2:6686073/1‑139 (MQ=255)

                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTCCCTGCCAGCACCGAGTGGTAATTATCTCCGACCAAAATGGCGTCCGCCGGCGCGCTTCCTAGCTGTTTCAGCGCAAAAAGCACAGGCTCGGGAt                             >  2:3701982/1‑140 (MQ=255)

                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTCCCAGCCAGCACAAAGGGGGAATTATATCCGACCAAAAAGGCTTACGCCGGCGCGCGTCCTAGCGGGTTCAGCGCTAAAAGCACACGCACGGGAt                             >  2:1058086/1‑140 (MQ=255)

                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTGCCTGCCAGCACATCGTGGTAAATATATCCGACCATAATGGGTTCCGCCGGGTGGGTTCCTAGCTGTTTCAGCGCGAAAAGCACAGGCTCggggt                             >  2:2907997/1‑138 (MQ=255)

                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  1:2580991/140‑1 (MQ=255)

                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  1:379375/140‑1 (MQ=255)

                                                                                                                     cctCCCTGGGACTCCGGCCGTTTTTGTCCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAATCACAGGCTCGGGATCAGGCt                       <  1:4404168/138‑1 (MQ=255)

                                                                                                                     cGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCt                       <  2:4285780/140‑1 (MQ=255)

                                                                                                                       tCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTGCCTGCCAGCCAATCGTGGTAATTATCTCCGACCATAATGGCTTACGCCGGCTCGCGTGCAAGCTGTTTGAGCGCTAAAAGCACAGGCTCGGGCTCAGGCttt                     >  2:4890529/1‑140 (MQ=255)

                                                                                                                         cATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTCCCTGCCAGCACATCGGGGTAATTATCTCCGCCCATAATGGCTTCCGCCGGCGCGCTTCCAAGCTGTTTCAGCGCGAAAACCACCGGCTCGGGCTCAGGCTTTGc                   >  2:4122565/1‑140 (MQ=255)

                                                                                                                         cATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCCTAATTGCTTCCGCCGGCTCGCGTCCGAGCTGTTTCAGCGCTAAAAGCAAAGGCTCGGGATCAGGCGTTGc                   >  2:4494238/1‑140 (MQ=255)

                                                                                                                         cATGCGACTCCGGCCGTTTTTGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                   <  1:4332898/140‑1 (MQ=255)

                                                                                                                           tGCGACTCCGGCCGTGTTTTTTACGGGGTTTTTCCCTGCCCGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTAGCTTACTAGCTGTTTAATCGCTGAAAGCCCACGCTCGGGTTCAGGCGTTTCCa                 >  2:5489949/1‑140 (MQ=255)

                                                                                                                               aCTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGt             <  2:1390852/140‑1 (MQ=255)

                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  1:4058105/140‑1 (MQ=255)

                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  1:1986786/140‑1 (MQ=255)

                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  2:1550103/140‑1 (MQ=255)

                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  1:537889/140‑1 (MQ=255)

                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  2:5950659/140‑1 (MQ=255)

                                                                                                                                  ccGGCCGTTTTTGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  2:5667391/140‑1 (MQ=255)

                                                                                                                                   cGGCCGTTTTTGTTGCGGCGTTTTTACCTGCCCGCACAACGGGGTAATTATCTCCGACCAAAATGGCTTCCCCCCGCGCGCGTCCGAGCGGTTTGAGCGCAAAAAGCACCGGCTCGGGATCAGGCTTTGCCATCGTccca         >  2:3545717/1‑140 (MQ=255)

                                                                                                                                     gCCGTTTTTGTTGCGGCGTTTTTGCCTGCCAGCACCACGTGGTAAATATCTCCGCCCAAAATTGCTTACGCCGGCACGCTTACTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATAACGCTTTGGCATAGTCAcata       >  2:618877/1‑139 (MQ=255)

                                                                                                                                     gCCGTTTTTGTTCCGGCGTGTTTCCCTTCCAGCACATCGTGGTAAGTATCTCCGACCAAAATGGCTTACGCCGGCTCGCGTCCTAGGGGGTTAAGCGCTAAAAGCACAGGCGCGGGATCAGGCTTTGGCATCGTCCcatc       >  2:7540040/1‑140 (MQ=255)

                                                                                                                                     gCCGTTTTTGTTACGGCGTTTTTCCCTGGCCGCACATCGTGGTAATTATCTCCGCCCATAATGGCTTACGCCGGCTCGCTTGCGAGCTGGTTAAGCGCTAAAAGCACAGGCTCGGGATCAGGCTGTGCGATCGTgacagg       >  2:6289465/1‑138 (MQ=255)

                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  2:6541713/140‑1 (MQ=255)

                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  1:5989963/140‑1 (MQ=255)

                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  1:705679/140‑1 (MQ=255)

                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  1:5647279/140‑1 (MQ=255)

                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  1:6183137/140‑1 (MQ=255)

                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  2:439292/140‑1 (MQ=255)

CGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAA  >  NZ_CP034943/3394222‑3394499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`