Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,962 C→A 13.7% M208I (ATG→ATT degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9620CA13.7% 967.8 / 39.8 495M208I (ATG→ATTdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base C (156/241);  new base A (86/2);  total (252/243)
Fisher's exact test for biased strand distribution p-value = 1.51e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 518 total aligned reads displayed.

TTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAAT  >  NZ_CP034943/3446823‑3447100
                                                                                                                                           |                                                                                                                                          
ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTAcattc                                                                                                                                            >  2:4852904/1‑140 (MQ=255)
ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAcattc                                                                                                                                            >  2:1382226/1‑140 (MQ=255)
ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAcattc                                                                                                                                            >  1:5508964/1‑140 (MQ=255)
ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAcattc                                                                                                                                            >  2:230509/1‑140 (MQ=255)
        ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                    >  2:5230051/1‑140 (MQ=255)
        ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGAACACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                    <  2:2173645/140‑1 (MQ=255)
         tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCTTTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGc                                                                                                                                   >  1:53743/1‑140 (MQ=255)
         tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGc                                                                                                                                   >  1:7474469/1‑140 (MQ=255)
          aTTTTAAGGGAAACGGCAGCCTAAAAAAAGACCGGCCCAGTAAAAGGCAAGTATCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                  <  2:7663260/140‑1 (MQ=255)
          aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                  <  1:3258661/140‑1 (MQ=255)
          aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                  <  1:2879344/140‑1 (MQ=255)
          aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                  <  2:2863775/140‑1 (MQ=255)
           ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATAGTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCaa                                                                                                                                 >  2:1146783/1‑140 (MQ=255)
           ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaa                                                                                                                                 >  2:2391357/1‑140 (MQ=255)
           ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaa                                                                                                                                 >  2:1884282/1‑140 (MQ=255)
           ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAAattttttaaa                                                                                                                                 >  1:683398/1‑136 (MQ=255)
               aaGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAata                                                                                                                             <  1:1985424/140‑1 (MQ=255)
                aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                            >  2:3244249/1‑140 (MQ=255)
                 gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTGAAATTCATTTTTTGCAAAAAAtt                                                                                                                           >  1:5849461/1‑140 (MQ=255)
                   gAAAGGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGc                                                                                                                         >  2:4727570/1‑140 (MQ=255)
                        ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCTcaca                                                                                                                    >  2:176505/1‑140 (MQ=255)
                                  aaacaGACCTGACCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCa                                                                                                          <  1:4259563/140‑1 (MQ=255)
                                       gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTAAATTTTTTCAAAATATTTCTCACATGGTTTTTGACGGtt                                                                                                     >  1:5014876/1‑140 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  1:3526074/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  1:1019589/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  2:692193/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 >  2:2790449/1‑140 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  2:5846417/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  1:5678097/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  1:3853020/140‑1 (MQ=255)
                                           tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                 <  1:6592924/140‑1 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCCGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTGCAAAAAAATGCTCAAATTGTTTTTCAAGGttttttaa                                                                                               >  1:272241/1‑136 (MQ=255)
                                             cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAACTTTTTTCAAAATATTTCTCACAATGTTTTTAACGGttttgtaa                                                                                               >  1:627491/1‑136 (MQ=255)
                                                      aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                      <  2:6567427/140‑1 (MQ=255)
                                                       ggCAAGACTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTACGGTCGTTTAAATTCATTTTTTGCAAAAAATTGCTCACATTTTATTTAACGGTTTTCACAATCATAAACa                                                                                     >  2:5346227/1‑140 (MQ=255)
                                                           aGTCTCTTATTATCCTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                 <  1:3935636/140‑1 (MQ=255)
                                                           aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                 <  2:5969062/140‑1 (MQ=255)
                                                           aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGAGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                 <  1:1588756/140‑1 (MQ=255)
                                                           aGTCTCTTATTATACTATCTAATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                 <  1:7556546/140‑1 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAAATTGTCACATGGTTTTTCACGGTTTTATCAATGAAAAACAATGAt                                                                                >  1:3657117/1‑140 (MQ=255)
                                                            gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTAAAAATATTTCTCAAAAGGTTTTTAACGGTTTTATCAATGATAAACAATGAt                                                                                >  1:1365402/1‑140 (MQ=255)
                                                                 ttattaTACTATCTCATTTCTACCCAGCCATTTTTAATTGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGGAAAAAATTGATCACAAGGTTTTTAACCGTTTTATCACTAATAAACAATGAttagcc                                                                           >  1:3143027/1‑136 (MQ=255)
                                                                   attatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTATCACTGATAAAAAAAGATTCAccca                                                                         >  2:2532046/1‑138 (MQ=255)
                                                                       taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAGATTCATTTTTTTCAAAAAATTTCTCACAATGTTTTTAACCGTTTTATCACTTATAAAAAAATATTAACCaaaaac                                                                     >  1:1209262/1‑136 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTAACTGATAAACAATGATTCAACAATACCGCgg                                                                 >  2:7317217/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCTCACATGGTTTTTCACGGTTTTATCACAGATAAACAATGATTAACCAATAACGCgg                                                                 >  2:3040100/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAATTTCTCAAAAGGTTTTTAAAGGTTTTGTCACAGTTAAAAAAATATTCAACAAAACCCCgg                                                                 >  1:4167873/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATTAATAAACAATACCGCgg                                                                 >  2:6161268/1‑140 (MQ=255)
                                                                           aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCAATTTTTGCCAAAAATTTCTCACATGGGTTTTAACGGTTTTGTCACTTATAAACAAACATTAAGCACAACCGCgg                                                                 >  1:2561122/1‑140 (MQ=255)
                                                                                 tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAATTGATTTTTTGCAAAATATTTCGCACAGGGTTTTTCAAGGTTTTATATCTTATTAACAATGATTCACCAAAACCGCCGTTGCtt                                                           >  1:7249573/1‑140 (MQ=255)
                                                                                 tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCtt                                                           >  2:3325417/1‑140 (MQ=255)
                                                                                 tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAAAAATGCTCACAAGGTTTTTCACGGTTTTCTCACTGATAAACCATTATTAACCAAAACCGCggttggtt                                                           >  1:7662052/1‑140 (MQ=255)
                                                                                        ccAGCGATTTTTAATTGCGACCACAACGGCTTGCGTCCGTTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGt                                                    <  1:2261439/140‑1 (MQ=255)
                                                                                        ccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGt                                                    <  1:7165819/140‑1 (MQ=255)
                                                                                          aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAATTCTCACAAGGTTTTTAACGGTTTTTTAACTGATAAAAAAATATTGACCAAAAACGCGGTTGCTTTTTACGTCt                                                  >  1:6914095/1‑140 (MQ=255)
                                                                                          aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCAATCATTTTTTGCAAAATATTTCTCACATGGTTGTTCACGGGTTTCTCACTTATAAACAAAGATTGACCCAAACCCCGGGTGGGTTTTACGTCt                                                  >  1:5175104/1‑140 (MQ=255)
                                                                                          aGCCATGTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTGAAAATATTTCTCGCCTGGTTTTTGACCGTTTTCTGACTGGTAAAAAAAGATTCACCAATAACGCGGTTGGTTTTTGCGTCt                                                  >  1:2017537/1‑140 (MQ=255)
                                                                                           gCCATTTTTAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTg                                                 <  2:5394901/140‑1 (MQ=255)
                                                                                            ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTTCAAAATATTGCTCCCATTGTTTTTCACCGTTTTGTCACTGTTAAACAATGATTAACCAAAACCGCGGTTGCGTTTTGCGTCTGc                                                >  1:3335125/1‑140 (MQ=255)
                                                                                            ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGc                                                <  1:5956968/140‑1 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                               <  1:927429/140‑1 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                               <  1:2112847/140‑1 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                               <  2:2077089/140‑1 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                               <  1:5278408/140‑1 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCa                                               <  1:371571/140‑1 (MQ=255)
                                                                                             cATTTTTAATGGCGACCACAACGGCTGGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                               <  2:4347644/140‑1 (MQ=255)
                                                                                                ttttAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                            <  2:5737830/140‑1 (MQ=255)
                                                                                                ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                            <  2:1022376/140‑1 (MQ=255)
                                                                                                     aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                       <  1:6108129/140‑1 (MQ=255)
                                                                                                     aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                       <  2:6767873/140‑1 (MQ=255)
                                                                                                     aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                       <  2:4072450/140‑1 (MQ=255)
                                                                                                     aTGGCGACCACAACGGCTTGCGGCCGGTCGATTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGAGAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTg                                       <  2:6380954/140‑1 (MQ=255)
                                                                                                      tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTGGGACTGATAAACAAAGATTAACCCAGACCGCGGTTGCGTTTTACGTCTGCACGCATCTgc                                      >  2:1080400/1‑140 (MQ=255)
                                                                                                      tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTGACCAAAAACGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                      >  2:3693650/1‑140 (MQ=255)
                                                                                                      tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTAACAAAAACCGCGGTTGCTTTTTCAGTCTGCAAGCATCTgc                                      >  2:843349/1‑140 (MQ=255)
                                                                                                      tGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATGTTTTGCAAAATATTGCTAACATTGTTTTTCACGGTTTTCGCACTGATAAACAATGATTAACCAAATCCGCGGTTGCTGTTTCCGTCTGCAACCATCTgc                                      >  2:3497621/1‑140 (MQ=255)
                                                                                                        gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                    <  1:4727570/140‑1 (MQ=255)
                                                                                                        gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                    <  1:7052052/140‑1 (MQ=255)
                                                                                                         cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                   <  1:1884282/140‑1 (MQ=255)
                                                                                                         cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCCTTTTCCGTCTGCAAGCATCTgcagc                                   <  1:2790449/140‑1 (MQ=255)
                                                                                                          gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAACCAATAACGCGGTTGCTTTTTCCGTGTGCAAGCATCTgcagca                                  >  2:6289857/1‑140 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                       <  1:5575827/122‑1 (MQ=255)
                                                                                                                       tGCGTCCGGTCGTTTAAAATAATTTTTTGCAAAATATTGGTCACATGGTTTTTAAAGGGTTTCTCACTGATAAACAATGATTCACAAATAACGCGGGTGCGTTTTGCGTCTGGAATGATGTGCCGCACTTCACATTcccc                     >  2:2989312/1‑139 (MQ=255)
                                                                                                                        gCGTCCGGTCGTTTACATTCATTTTTTGCAACATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCccgc                    <  1:1782789/140‑1 (MQ=255)
                                                                                                                        gCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCccgc                    <  2:1995541/140‑1 (MQ=255)
                                                                                                                         cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                   <  1:6658766/140‑1 (MQ=255)
                                                                                                                          gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                  <  1:868205/140‑1 (MQ=255)
                                                                                                                          gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                       >  2:3743643/1‑119 (MQ=255)
                                                                                                                          gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                  <  1:4436548/140‑1 (MQ=255)
                                                                                                                              ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt              <  1:6464694/140‑1 (MQ=255)
                                                                                                                              ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt              <  2:5066984/140‑1 (MQ=255)
                                                                                                                                 cGTTTACATTCATTTTTTGCAACATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTGCCGTCTGCAAGCATCTGCAGCACTTGCCATTCCCGCCGAGTTaaa           <  2:1518241/140‑1 (MQ=255)
                                                                                                                                 cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa           <  1:7350380/140‑1 (MQ=255)
                                                                                                                                 cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa           <  2:7057515/140‑1 (MQ=255)
                                                                                                                                 cGTTTACATTCATTTTTAGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa           <  2:5647708/140‑1 (MQ=255)
                                                                                                                                 cGTTTAAATTAATTTTTTGAAAAATATTGCTCAGATTGTGTTTAACTGTTTTATCACTGATAAACAATGATTAACCAAGTCCGCGGTTTCTTTTTCGGTCTGAAAGCATCAGCAGCACTTCACATTCCCGCCGAGTTaaa           <  1:1269729/140‑1 (MQ=255)
                                                                                                                                 attttACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa           <  2:4591981/138‑1 (MQ=255)
                                                                                                                                   tttACATTAAATTTTTGCAAAATATTTCTCAAATGGTTTTTCACGGTTTTCAAACTTCTAAACAATGATTAACCAATAACCCGGTTGCGTTTTACGTCTGCCAGCAACTGCCCCACATCACAATCACGCCCAGGTAAAat         >  2:737711/1‑140 (MQ=255)
                                                                                                                                        attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa    <  1:1423396/140‑1 (MQ=255)
                                                                                                                                          tcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAAt  >  2:7569953/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAAT  >  NZ_CP034943/3446823‑3447100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: