Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,970 G→T 17.7% Q206K (CAA→AAA)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9700GT17.7% 1046.1 / 92.0 537Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (155/272);  new base T (108/2);  total (263/274)
Fisher's exact test for biased strand distribution p-value = 5.50e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 544 total aligned reads displayed.

CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108
                                                                                                                                            |                                                                                                                                          
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGAACACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                            <  2:2173645/140‑1 (MQ=255)
 tATTGAACGGGAAACGGCAGCCTAAAATAAGACCTGCCCAGTAAAAGGCAAGTCTCTAATTATACTATCACAGATCTACCCAGCCATTTTTAATGGCCACCACAACCGCTTGCGTCCCGTCCTTTACATTCATTTTATGc                                                                                                                                           >  2:2121663/1‑140 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:952359/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:886106/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:3258661/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:2879344/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTATACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  2:1365083/140‑1 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCACGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGGACCTTCATTTTTTGCAAAatat                                                                                                                                    >  1:203593/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAatat                                                                                                                                    >  2:2363281/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                                    <  1:785133/140‑1 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTTCAAAatat                                                                                                                                    >  2:5965806/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTATGATTATACTATCTCATTTCTCCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAatat                                                                                                                                    >  2:5359607/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGAAAAATGCAAGTCTCTTATTATAATATCTCATTTCTACCCAGCCATTTATAAAGGAGACCACAACGGCTTGCGTCCGCTCGTGTAAAATAAATTTTTTAaaaaaaa                                                                                                                                    >  1:6013046/1‑137 (MQ=255)
         gtgAAACGTCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTATACCCAGAAATTTTTAATGGCGACCACAACGGCTTTCGTAATGTCTTTTACATTCATTTTTTTAAAAataat                                                                                                                                   >  2:6257145/3‑138 (MQ=255)
             aaCGGCAGCCTACAAAAAGACCTGCCCCGTAAAAGGCAAGTCTCTTATGCGACGATCTCGTGTCGACCCCGCCATTTTGACTGGCGCCCCCCACGGCGTGCGGCCGGTCGGGTACTTTCATTGTTTGCCAAATTTTGcgc                                                                                                                               >  2:3420606/1‑138 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTGAAAATATTtctc                                                                                                                               >  1:7183518/1‑140 (MQ=255)
              aCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTca                                                                                                                              <  1:2467697/140‑1 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATAATTCTcaca                                                                                                                            >  2:1027191/1‑140 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCTcaca                                                                                                                            >  2:176505/1‑140 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                            <  1:7533567/140‑1 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                            <  1:505997/140‑1 (MQ=255)
                    gCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCAGTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCGCACATGGt                                                                                                                        >  2:5495704/1‑140 (MQ=255)
                     ccTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTGATTATAGTATCTCATTTCTACGCAGCCATTTTTCATGGCGACCACACCGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCGAAATATTGATGACAggggt                                                                                                                       >  1:6355848/1‑138 (MQ=255)
                     ccTAAAAAAAGAACTGCCCAGTAAAAGGCAATTCTCTTATTGTACTATCTAATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGTCGTGTAGATAGCTGTTTTGCAGAATATTGCTCACATGGtt                                                                                                                       >  2:3061287/1‑140 (MQ=255)
                          aaacaGACCTGACCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCa                                                                                                                  <  1:4259563/140‑1 (MQ=255)
                               gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGtt                                                                                                             >  2:7305171/1‑140 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  2:5846417/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:3853020/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  2:692193/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:3526074/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  2:2993465/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:1019589/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAATATTGATCACATGGTTTTTCACCGTTTtctaa                                                                                                       >  2:4861323/1‑138 (MQ=255)
                                             aaGGCAAGTCTCTTATTATACTATCTCATGTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATaaa                                                                                               <  1:5772268/140‑1 (MQ=255)
                                              aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                              <  2:7093669/140‑1 (MQ=255)
                                              aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                              <  2:6567427/140‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:7286109/140‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGAGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:1588756/140‑1 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTAAAAATATTTCTCAAAAGGTTTTTAACGGTTTTATCAATGATAAACAATGAt                                                                                        >  1:1365402/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAAATTTTTTCAAAAAATTTCTCACAAGGTTTTTAAACGATTTATAACAGATAAAAAATTAt                                                                                        >  2:3911707/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCAATAATTTTTTTCAAAAAATTGGTCACATGGTTTTTGACGGTTTTATCACTGATAAAAAAAGAt                                                                                        >  1:5328249/1‑140 (MQ=255)
                                                        ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAc                                                                                    <  1:3144921/140‑1 (MQ=255)
                                                         ttattaTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCTCACAAGGTTTTTCACGGTTTTTTGAATGATAAACAAAGATTCAcc                                                                                   >  1:6613267/1‑140 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAGATTCATTTTTTTCAAAAAATTTCTCACAATGTTTTTAACCGTTTTATCACTTATAAAAAAATATTAACCaaaaac                                                                             >  1:1209262/1‑136 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTGAAAAAATTTCTCACAAGGTTTTTAACGGTTTTGTCACAGATAAAAAATGATTCACCaaaaaa                                                                             >  1:682320/1‑136 (MQ=255)
                                                                  tttctcATTTCTACCCAGCCATTTTTAATGGCGAACACAACGGCTTGCTTCCGTTCGTTTAAATTCATTTTTTGCAAATTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACcgcg                                                                          <  1:3654472/138‑1 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTTCGTCCGGTCGTTTACATTAATTTTTTGCAAAAAATTTCTAAAATGGTTTTTCAAGGTTTTCTCACTGATAAAAAATCATTAACCAATaaaccgg                                                                         >  1:6709804/1‑134 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATAAATTTTTTGAAAAAAATTATCACATTGTTTTTCAAGGTTTTGTAACTGATAAAAAAAAATTCACAAAAAcacccg                                                                         >  1:1993516/1‑135 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCAAATGGTTTTTAAAGGTTTTATCACTGATAAACAATGATTCACCAATAACCCgg                                                                         >  2:4759844/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAATTTCTCAAAAGGTTTTTAAAGGTTTTGTCACAGTTAAAAAAATATTCAACAAAACCCCgg                                                                         >  1:4167873/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAATAATGCTCAAAAGGGTGTTAAAGGTTTTCTCACTTATAAACAAATAGTAACCAATACCGCgg                                                                         >  2:5664897/1‑140 (MQ=255)
                                                                      tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTAACAATGTTTTTCACGGTTTTATAACAGATAAAAAATGATTAACCAAAACCGCGGTTg                                                                      >  2:2438571/1‑140 (MQ=255)
                                                                      tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATAATTCTCACATTGTTTTTAACGGTTTTATAAATGATAAACAATTATTAAACAATAACGCGGTTg                                                                      >  2:5302242/1‑140 (MQ=255)
                                                                      tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTCACCGTTTTATCACTGATAAACAATGATTCAACAAAAACGCCGTTg                                                                      >  2:2808658/1‑140 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCAATCATTTTTTTCAAAATAATGCTCCCAATGTTTTTCACGGTTTTCTAACTGATAAACAATGAATAACCAAAACCGCGGTTGCtt                                                                   >  1:4936187/1‑140 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATAGTGCTCAAATGGTTTTTAACGGTTTTCTCACTGATAAACAATGTTTGACCAATAACGCGGGTgggt                                                                   >  2:2933153/1‑137 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATAATGCTAACATGGTTTTTAACGGTTTTATAAATGATAAAAAATTATTAACCAATAACGAGGTTGCtt                                                                   >  2:4213411/1‑140 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTATAAATGATAAAAAATTATTAAACAATAACGCGGGTTCtt                                                                   >  2:2754213/1‑140 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                              <  2:7673968/140‑1 (MQ=255)
                                                                                ccAGCGATTTTTAATTGCGACCACAACGGCTTGCGTCCGTTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGt                                                            <  1:2261439/140‑1 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTAGCAATGATAAAAAATTTTTAGACAAAAACGCGGTTGCTTTTTGCGTCt                                                          >  2:5109234/1‑140 (MQ=255)
                                                                                    ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTTCAAAATATTGCTCCCATTGTTTTTCACCGTTTTGTCACTGTTAAACAATGATTAACCAAAACCGCGGTTGCGTTTTGCGTCTGc                                                        >  1:3335125/1‑140 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  2:2009733/140‑1 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  1:5426734/140‑1 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCa                                                       <  1:371571/140‑1 (MQ=255)
                                                                                      aTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCaa                                                      <  1:5910947/140‑1 (MQ=255)
                                                                                      aTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCaa                                                      <  2:6370087/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  2:5737830/140‑1 (MQ=255)
                                                                                             aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTg                                               <  2:3285034/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:2717309/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  2:1181385/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:3951456/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:2134265/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:7052052/140‑1 (MQ=255)
                                                                                                 cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCCTTTTCCGTCTGCAAGCATCTgcagc                                           <  1:2790449/140‑1 (MQ=255)
                                                                                                 cGACCACAACGGCTTGCGTCCGGTAGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTgcagc                                           <  1:1403421/140‑1 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                          <  1:5210530/140‑1 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTGTACATTCCTTTTTTGCAAACTATTGCTCACATGGTTTTTCACGGTTTTCTGAGTGATAAAAAATTATTCACCAATACCGCGGTTGCGTTTTCCGTCTGCCAGCATCTggagca                                          >  2:4593033/1‑140 (MQ=255)
                                                                                                             cTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTATAACTGATAAACAATGATTCACCAATACCGCGGTTGGTTTTTACGTCTGCAAGCAACTGCAGCACTTCACAttac                               >  2:849263/1‑138 (MQ=255)
                                                                                                               tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                             <  1:1920657/140‑1 (MQ=255)
                                                                                                               tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                             <  1:6218228/140‑1 (MQ=255)
                                                                                                                gCGTCCGGTCGTTTACATTCATTTTTTGCAACATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCccgc                            <  1:1782789/140‑1 (MQ=255)
                                                                                                                 cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                           <  1:6500502/140‑1 (MQ=255)
                                                                                                                  gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                          <  1:4436548/140‑1 (MQ=255)
                                                                                                                  gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                          <  1:7447390/140‑1 (MQ=255)
                                                                                                                    ccGGTAGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                        <  2:6114150/140‑1 (MQ=255)
                                                                                                                      ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACCTTCCCGCCGAGtt                      <  2:1100837/140‑1 (MQ=255)
                                                                                                                       gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                     <  1:5521946/140‑1 (MQ=255)
                                                                                                                       gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATTATTAACCAATAACGCGGTTGCTTTTTACGTCTGCAAGCATCTGCACCACTTCACAATACCCCCGAGTTa                     >  2:369475/1‑140 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAACATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTGCCGTCTGCAAGCATCTGCAGCACTTGCCATTCCCGCCGAGTTaaa                   <  2:1518241/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:7627166/140‑1 (MQ=255)
                                                                                                                         cGTTTAAATTAATTTTTTGAAAAATATTGCTCAGATTGTGTTTAACTGTTTTATCACTGATAAACAATGATTAACCAAGTCCGCGGTTTCTTTTTCGGTCTGAAAGCATCAGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:1269729/140‑1 (MQ=255)
                                                                                                                           tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  2:4374550/140‑1 (MQ=255)
                                                                                                                           tttACATTAAATTTTTGCAAAATATTTCTCAAATGGTTTTTCACGGTTTTCAAACTTCTAAACAATGATTAACCAATAACCCGGTTGCGTTTTACGTCTGCCAGCAACTGCCCCACATCACAATCACGCCCAGGTAAAat                 >  2:737711/1‑140 (MQ=255)
                                                                                                                           attACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  2:1923012/139‑1 (MQ=255)
                                                                                                                                 aatcattTTTTGCACACTATTGATCACCTGGTTTTTCACGGTTTTCTCACTGATACACAATGCTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGtc            <  1:5611730/138‑2 (MQ=255)
                                                                                                                                   tcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAAt          >  2:7569953/1‑140 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:6917473/140‑1 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  >  2:6759565/1‑140 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:4707573/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: