Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,447,038 T→G 11.6% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,447,0380TG11.6% 1075.3 / 39.2 551N183T (AAC→ACC) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (205/257);  new base G (18/70);  total (223/328)
Fisher's exact test for biased strand distribution p-value = 1.77e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 559 total aligned reads displayed.

TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176
                                                                                                                                            |                                                                                                                                          
  tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTAACAATGTTTTTCACGGTTTTATAACAGATAAAAAATGATTAACCAAAACCGCGGTTg                                                                                                                                          >  2:2438571/1‑140 (MQ=255)
  tcATTTCTACACAGACATTTTTAATGTCTAAAACAACTTATTGCGTCCTTTATTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTTACGTTTTTCTCACTGATAAAAAATTATTCACCAATACCGCGGTTg                                                                                                                                          <  2:586551/140‑1 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCAATCATTTTTTTCAAAATATTGCTCACAATGTTTTTAACGGTTTTATCAATTATAAAAAAAGATTAAACAAAAACCCGGTTGCtt                                                                                                                                       >  1:5971516/1‑140 (MQ=255)
          aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTAACATGGTTTTTCACGGTTTTATCACTGATAAAAAATGATTCACCAATACCGCGGTTGCtttttac                                                                                                                                  >  2:5336603/1‑138 (MQ=255)
            ccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGt                                                                                                                                <  1:7165819/140‑1 (MQ=255)
              aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCCAAAAATTTCTCACATGGTTTTTCACGGGTTTGTAACGTATAAACAAAGAATAACCAAAACCGCGGTTGCGTTTTGCGTCt                                                                                                                              >  1:228580/1‑140 (MQ=255)
              aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCAATCATTTTTTGCAAAATATTTCTCACATGGTTGTTCACGGGTTTCTCACTTATAAACAAAGATTGACCCAAACCCCGGGTGGGTTTTACGTCt                                                                                                                              >  1:5175104/1‑140 (MQ=255)
              aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACCTTCATTTTTTTCAAAATCTTTCTCCAATGGTTTTTCAACGTTTTATGACTGTTAAAAAAATGATGACCAATACCCCGGTTGGGTTTTGCGGCt                                                                                                                              >  1:3867182/1‑140 (MQ=255)
              aGCCATGTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTGAAAATATTTCTCGCCTGGTTTTTGACCGTTTTCTGACTGGTAAAAAAAGATTCACCAATAACGCGGTTGGTTTTTGCGTCt                                                                                                                              >  1:2017537/1‑140 (MQ=255)
               gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTg                                                                                                                             <  2:2235421/140‑1 (MQ=255)
                ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATAATTTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTGTAACTGATAAACAAAGATTGACCCATAACCCGGTTGCTTTTTACggcggc                                                                                                                            >  1:5737830/1‑135 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCCCCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  1:709007/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCa                                                                                                                           <  1:371571/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  2:5209384/140‑1 (MQ=255)
                  aTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTGTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCaa                                                                                                                          <  2:1929878/140‑1 (MQ=255)
                    ttttAATGGCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:204407/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:6878001/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:6177202/140‑1 (MQ=255)
                         aTGGCGACCACAACGGCTTGCGTCCGGTCGTGTACCTTAAATTTTTTCAAAATATTTCTGACATGGTTTTTGACGGGTTTCTGACTGGTAAAACAATCTTCACCAATACCCCGGTTGCTTTTTACGTGTGCAAGCAGCTg                                                                                                                   >  1:3476895/1‑140 (MQ=255)
                         aTGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTGACGGTTTTTTCACTGATAAACAATGATTCACCAAAACCCCCGTTGCTTTTTCCGTCTGCAAGCATCTg                                                                                                                   >  1:4264266/1‑140 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACCGTTTTCTCACTGATAAACAATGAGTCACCAAAACCGCGGTTGCGTTTTCCCTATGCAAGccgatgc                                                                                                                  >  2:4813441/1‑134 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTAACAAAAACCGCGGTTGCTTTTTCAGTCTGCAAGCATCTgc                                                                                                                  >  2:843349/1‑140 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  1:2717309/140‑1 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  1:2134265/140‑1 (MQ=255)
                             cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCACTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                                                                                               >  2:1098951/1‑140 (MQ=255)
                             cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCCTTTTCCGTCTGCAAGCATCTgcagc                                                                                                               <  1:2790449/140‑1 (MQ=255)
                              gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                                                              <  1:7498730/140‑1 (MQ=255)
                              gACCACAACGGCTTGCGTCCGGTCGTGTACATTCCTTTTTTGCAAACTATTGCTCACATGGTTTTTCACGGTTTTCTGAGTGATAAAAAATTATTCACCAATACCGCGGTTGCGTTTTCCGTCTGCCAGCATCTggagca                                                                                                              >  2:4593033/1‑140 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCCTGGTTGGCACAATATTGCTCACAGGGTTTTTCACGGTTTTCTCCCTGATAACCAATGATTCCCCCCTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGGCccg                                                                                                 <  2:2142955/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                                                                 <  2:3347053/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                                                                 <  1:6218228/140‑1 (MQ=255)
                                             cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                                                               <  1:1244107/140‑1 (MQ=255)
                                             cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTATGCAAGCATCTGCAGCACTTCACAGTCccgcc                                                                                               <  1:918601/140‑1 (MQ=255)
                                                   gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATTATTAACCAATAACGCGGTTGCTTTTTACGTCTGCAAGCATCTGCACCACTTCACAATACCCCCGAGTTa                                                                                         >  2:369475/1‑140 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAACATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTGCCGTCTGCAAGCATCTGCAGCACTTGCCATTCCCGCCGAGTTaaa                                                                                       <  2:1518241/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:6053441/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:4394998/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:7350380/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:7288869/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                                                                                       <  2:2619004/140‑1 (MQ=255)
                                                       tttACATTCATTTTTTGCAAAAAATTGCTCACAAGGTTTTTAAACGTTTTCTCACAGATAAACAATGATTCACCAATAACGCGGGTGCAATTTACCTCTGCAAGCATATCCAGCACTTCAAATTCCCGCCGAGTTAAAat                                                                                     >  2:7660925/1‑140 (MQ=255)
                                                            attcattTTTTGCAACATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                                                                <  2:5147382/140‑1 (MQ=255)
                                                            aatcattTTTTGCACACTATTGATCACCTGGTTTTTCACGGTTTTCTCACTGATACACAATGCTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGtc                                                                                <  1:5611730/138‑2 (MQ=255)
                                                              tcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAAt                                                                              >  2:7569953/1‑140 (MQ=255)
                                                                attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                                                            >  2:933949/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACACAGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCAGCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:2985216/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:6759565/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:458019/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:4707573/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:1382226/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:6917473/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:119869/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:2994938/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:5336603/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:3981922/140‑1 (MQ=255)
                                                                        cAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCACGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGa                                                                    >  2:2872148/1‑140 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  1:1027191/140‑1 (MQ=255)
                                                                                 gCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:441286/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:6445653/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:99082/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:3978772/140‑1 (MQ=255)
                                                                                   gcaccgggTTTTGCACGGTGTTCTCACTGATAAACTATGATTCCCCAATTCCGGGGTTGCTTTTTCCGTCGGAATGCATCTGCAGCACTTCACATTCCCGGCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         <  2:6378008/134‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                                                                           <  1:1666117/86‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                                                                           <  1:586812/86‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:688515/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:1534355/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:5682926/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1810277/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3628978/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATAGGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:3189892/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:710442/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:4326592/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGAGTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:6295545/140‑1 (MQ=255)
                                                                                       aTGGTTTGTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:6555990/140‑1 (MQ=255)
                                                                                       aTGGTTTGTCACGGTGTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:3143027/140‑1 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc                                                >  2:7435835/1‑140 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc                                                <  1:5032004/140‑1 (MQ=255)
                                                                                                 aCGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAgg                                           <  2:3797663/140‑1 (MQ=255)
                                                                                                     ttttCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:4111696/126‑1 (MQ=255)
                                                                                                     ttttCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:4111696/1‑126 (MQ=255)
                                                                                                          tcACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGa                                  <  2:5513260/140‑1 (MQ=255)
                                                                                                             cTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                >  2:5731485/1‑59 (MQ=255)
                                                                                                               gATAAACAATGCTTCTCCAGTACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTc                             >  2:2763173/1‑140 (MQ=255)
                                                                                                               gATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTc                             >  1:493404/1‑140 (MQ=255)
                                                                                                               gATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTc                             >  2:6524227/1‑140 (MQ=255)
                                                                                                               gATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTc                             >  2:6982604/1‑140 (MQ=255)
                                                                                                                    aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  1:2831961/140‑1 (MQ=255)
                                                                                                                    aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  2:7352993/140‑1 (MQ=255)
                                                                                                                         tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  2:5032582/75‑1 (MQ=255)
                                                                                                                         tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:139702/1‑140 (MQ=255)
                                                                                                                           aTTCCCCAATACCGCGGCTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTACACAGGCCCCCCGAGATAAAATATGTAATGGTCTGCGGATTTACGGATAGCCCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGcc                  <  1:7610510/140‑2 (MQ=255)
                                                                                                                           aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:6660231/1‑105 (MQ=255)
                                                                                                                           aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:1947141/1‑140 (MQ=255)
                                                                                                                           aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:5432104/1‑140 (MQ=255)
                                                                                                                           aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  2:323348/1‑140 (MQ=255)
                                                                                                                           aTTCACCAATACCCCGGTAGCTTTTTACGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCTGATTTCAGGAAAGACCTAATGCTGAGGGAGTGCAGAAAATCCGAttgttgaa                  >  2:3523688/1‑138 (MQ=255)
                                                                                                                             tCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAg                >  1:828667/1‑140 (MQ=255)
                                                                                                                                    tACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGcggcgg         >  2:1642954/1‑140 (MQ=255)
                                                                                                                                     aCCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGa        >  2:2486197/1‑140 (MQ=255)
                                                                                                                                          ggTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCg   >  1:2383169/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: