Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,496,384 G→T 5.4% I348I (ATC→ATA EO946_RS17995 ← glucosaminidase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,496,3840GT 5.4% 1149.0 / 16.7 412I348I (ATC→ATAEO946_RS17995glucosaminidase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (90/288);  new base T (32/2);  total (122/290)
Fisher's exact test for biased strand distribution p-value = 1.60e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

TTTCTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGAT  >  NZ_CP034943/3496245‑3496516
                                                                                                                                           |                                                                                                                                    
  tCTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGag                                                                                                                                    >  2:3822075/1‑139 (MQ=255)
  tCTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGAt                                                                                                                                    >  1:4452471/1‑140 (MQ=255)
     gctgTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCt                                                                                                                                 >  1:7352810/1‑140 (MQ=255)
          tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                            <  1:1222082/140‑1 (MQ=255)
          tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                            <  2:3001852/140‑1 (MQ=255)
          tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                            <  1:483484/140‑1 (MQ=255)
              ccAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACgt                                                                                                                        >  2:311823/1‑140 (MQ=255)
               cAGTTGCTTTTTTCGTGGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACgtg                                                                                                                       <  2:6117486/140‑1 (MQ=255)
                 gTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGGTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATTCTTGATTACGTGTa                                                                                                                     >  2:6032227/1‑140 (MQ=255)
                   tGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAt                                                                                                                   >  2:2695313/1‑140 (MQ=255)
                               tGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATTCCAttttttat                                                                                                       >  2:773715/1‑138 (MQ=255)
                                gCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCtt                                                                                                      <  2:853769/140‑1 (MQ=255)
                                     tACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGAAGACGTGTAATGCCATGTTTTATTTTTaa                                                                                                 >  2:1997784/1‑140 (MQ=255)
                                        ttttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                              <  1:52349/140‑1 (MQ=255)
                                        ttttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                              <  1:2129128/140‑1 (MQ=255)
                                         tttttCTGAGAAATGAAGGGTTCTGCTTTTTTTGCTTGATGCTCTCATAAATTCTGCGCCGTCGTTATTGGATATGATGTAGAAATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaagaa                                                                                             <  1:4590124/140‑1 (MQ=255)
                                              cTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAgtgt                                                                                        <  2:1380442/140‑1 (MQ=255)
                                                 agaAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATGGTTGGTGGCTTGTAATTCCATTTTTTATTTTTtaagtaaatttgta                                                                                     >  1:901925/1‑133 (MQ=255)
                                                         agGCTGCGGCATTGTGTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATCGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGc                                                                             <  1:4766660/140‑1 (MQ=255)
                                                           gCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATTCAGGATGACGTGTAATTCCATTTTTTATTTTTAAATAAAATGTATATAtttttaat                                                                           >  2:4598892/1‑136 (MQ=255)
                                                           gCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                              >  2:5458624/1‑121 (MQ=255)
                                                           gCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTGTTTTGATGCTGGATGACGTGTAATGCCCTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGccg                                                                           >  2:4365941/1‑138 (MQ=255)
                                                                  cTTTGTTTGCTTGCTGCTCGCCTAAACTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCGGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGt                                                                    <  1:4078917/140‑1 (MQ=255)
                                                                  cTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGGAGACATGGTCGTTTTTTTGATGCTGGAGGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGt                                                                    <  2:3406165/140‑1 (MQ=255)
                                                                   tttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGtt                                                                   <  1:4233103/140‑1 (MQ=255)
                                                                   tttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGtt                                                                   <  1:5852452/140‑1 (MQ=255)
                                                                    ttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGa                                                                                                                                     <  1:7242283/73‑1 (MQ=255)
                                                                    ttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGa                                                                                                                                     <  1:6160391/73‑1 (MQ=255)
                                                                     tgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATGCTGGATGAAGTGTAATTCCATTTTTTGTTTTTAAAGAAAGTGTGTATCTTTTGGAGCTGGTGtttg                                                                 >  2:1096873/1‑139 (MQ=255)
                                                                      gtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTATTTTTAAAGAAAGTTTATATATTTTTCAGCTGCTGtttat                                                                >  2:6394512/1‑138 (MQ=255)
                                                                        ttgcttgcTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGtt                                                              <  1:5453158/140‑1 (MQ=255)
                                                                        ttgcttgcTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGtt                                                              <  1:1985832/140‑1 (MQ=255)
                                                                        ttgcttgcTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGtt                                                              <  1:1395883/140‑1 (MQ=255)
                                                                         tgcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTATTTTTAAAGAAATTGTGTATCTTTTTCAGCTGCTGTTTCGTTa                                                             >  2:1536738/1‑140 (MQ=255)
                                                                          gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                            <  2:3577799/140‑1 (MQ=255)
                                                                          gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                            <  2:2727237/140‑1 (MQ=255)
                                                                          gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                            <  2:123526/140‑1 (MQ=255)
                                                                          gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                            <  1:3325224/140‑1 (MQ=255)
                                                                            ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTTTTGTGGATTACGGGTAATTACAATTTTTTTTTTTAAATAAAGTTTGTATATTTTTGAGCTGCTTTTTGGTTaaaa                                                          >  1:2959108/1‑138 (MQ=255)
                                                                            ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                          <  2:3720313/140‑1 (MQ=255)
                                                                            ttgctGCTAGCCTAAAATCTGCTCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                          <  2:4530867/140‑1 (MQ=255)
                                                                            gtgctgcTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                          <  1:993894/139‑1 (MQ=255)
                                                                              gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                        <  1:6032433/140‑1 (MQ=255)
                                                                              gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                        <  2:2889473/140‑1 (MQ=255)
                                                                                tgctCGCATAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCtt                                                      <  2:4975197/140‑1 (MQ=255)
                                                                                      ccaaaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  1:7671768/137‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTACCCCCTTCATAtt                                                <  2:6480841/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  1:6664069/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  1:2096355/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  1:2177825/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  2:4452471/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  2:1811098/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  1:2695313/140‑1 (MQ=255)
                                                                                      ccTAAAATCTGCGCAGTCTTTATTGGATTGGCTGTAGACATTGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                <  2:7653490/140‑1 (MQ=255)
                                                                                       cTAAAATCTGCGCCGTCGTTATTGGATAGGCTTTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTATTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                               <  1:4376960/140‑1 (MQ=255)
                                                                                       cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                               <  1:2280970/140‑1 (MQ=255)
                                                                                       cTAAAATCTGCGCCGTCGTTATTGGAGAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                               <  1:6547760/140‑1 (MQ=255)
                                                                                        tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGTCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                              <  1:3745069/140‑1 (MQ=255)
                                                                                        tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                              <  2:2327622/140‑1 (MQ=255)
                                                                                        tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                              <  2:346018/140‑1 (MQ=255)
                                                                                        tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                              <  2:4725294/140‑1 (MQ=255)
                                                                                        tAAAATCTGCGCAGTCGTAATTGTATAGGCTGTAGACATGTTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTTCTGTTTCGTTAACCCCTTCATATTCa                                              <  1:6438800/140‑1 (MQ=255)
                                                                                         aaacTCTGCGCAGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  1:5903570/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  2:6551646/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  2:946495/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  2:5734409/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  1:5720943/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  1:2460442/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  1:3031856/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  2:126508/140‑1 (MQ=255)
                                                                                         aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                             <  1:319872/140‑1 (MQ=255)
                                                                                                 cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTTTGGTGGTTGTAGGGTAATTCCAATTTTTATTTTTAAATAAATTTTGTTTCGTTTGGAGCGGGTGGTTCGTTAAACCCATCAAATTAAATGTatatt                                     >  1:1793846/1‑139 (MQ=255)
                                                                                                 cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTTTGCTGGCTGACGTGTAATGCCATTTTTTCTTTTTAAATAAACTGTGTATGTTTTGGAGGTTCTGTTTAGTTAACCCCTTCATATTCACTGTatatt                                     >  2:4210187/1‑139 (MQ=255)
                                                                                                 cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTGTATCTTTTTCAGCTGCTGTTTCGTTAACCCCGTCATATTAACTGTATatg                                     >  2:1664092/1‑140 (MQ=255)
                                                                                                      tcgtTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGAtgtg                                <  1:5003836/140‑1 (MQ=255)
                                                                                                      tcgtTATTGGATAGGCTGTAGACAGGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTGTTTTTAAAGAAAGTTTGTTTCTTTTGGAGCTTCTTTTTTGTTAACCCCATGATATTCACTTTATATTAtgtt                                >  2:224848/1‑139 (MQ=255)
                                                                                                            ttGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTg                          <  2:7228849/140‑1 (MQ=255)
                                                                                                              agacaGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTc                        <  1:1516092/136‑1 (MQ=255)
                                                                                                                 tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                     <  1:3852223/140‑1 (MQ=255)
                                                                                                                 tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                     <  1:7139229/140‑1 (MQ=255)
                                                                                                                 acagcggTAGAAATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTATTATGAAAGAAAGTGTCGATCTTTTGCAGCTGCTGTTTCGTTCACCCCTTCATATTCACTGTATAGGATGTGCTTTTGTCAAc                     <  1:1654602/134‑1 (MQ=255)
                                                                                                                  aGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTATTTTTAAAGAAATTGTCTATCTTTTTCAGCTGCTGTTTAGTTAAACCCATCATATTCACTGTATATTATGTTCTTTTGTGAAcc                    >  2:5632602/1‑140 (MQ=255)
                                                                                                                    gCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAAGCCATTTTTTATTTTTTAAGAAAGTGTCTATCTTTTTCAGCTGGTGTTTCGTTAACCCCTTAATATTCACTGTATTTTATTTGCTTTTTTGAAccgg                  >  2:2582716/1‑138 (MQ=255)
                                                                                                                         aGACATGGTCGTTTTTTTGTTGCTGGATGACGTGTAAATCCCTTTTTTGTTTTTAAAGAAAGTGTGTATCTTTTTGAGCTTCTGTGTGGTTAACCCCGTGATATTCACTGTATATGTTTTGCTTTTTTCAACCTGGTGAt             >  2:334532/1‑140 (MQ=255)
                                                                                                                         aGACATGGTCGTTTTTTTGATGCTGGATTACGTGTAATTGCAATTTTTCTTTTTAAAGAAAGTGTGTATCTTTTTCACCTCCTGTTTCTGTAAACCCGTCCTATTAAGTGTATATTATTTGCTTTTTTCAACCAGTTGAt             >  2:6930822/1‑140 (MQ=255)
                                                                                                                         aGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGAt             <  2:1052141/140‑1 (MQ=255)
                                                                                                                         aGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGAt             <  2:7464693/140‑1 (MQ=255)
                                                                                                                          gACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATa            <  1:1046043/140‑1 (MQ=255)
                                                                                                                             aTGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACt         <  2:4263084/140‑1 (MQ=255)
                                                                                                                              tGGTCGTTTTTTTGATGCTGGATGGCGTGTAATGGCAATTTTTCTTTTTAAAGAAATTTTATATGTTTTGCAGCGGCTGTTTAGTTAACCCCATCATAATAACTGTATATGATGTGCTTTTTTAAAACGGTTGATaaatt        >  2:7082878/1‑137 (MQ=255)
                                                                                                                              tGGTCGTTTTTTTGATGCTGGATGCCGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGGGTAACTTTTTCAGCTGCTGTGTGGTTAACCCCTTCATATTAACTGTATATGATGTGCTTTTTTCAACCTGTTGATAAATg        >  2:6256802/1‑140 (MQ=255)
                                                                                                                               ggTCGTTTTTTTTATGGTGGATGAAGTGTAATTCCATTTTTTCTTTTTAAAGAAATTTTCTATATTTTGGAGCGGCTGTTTGGTTAAACCCATCATATTGACTGTTTATTTTGTGCTTTTGTGAACATGTTGATaaattc       >  2:2154543/1‑136 (MQ=255)
                                                                                                                               ggTCGTTTTTTTGTTTCGGGATGACGTGTAATGCCATTTTTTCTTTTTAAACAAAGTGTGTATGTTTTTCAGGGGCTGTTTCGTTAAACCCTTAATAGTCACTGTATATTATGTGAGTTTTTAAACCTGTTGATatttct       >  2:4018600/1‑135 (MQ=255)
                                                                                                                               ggTCGTTTTTTTGATTCGGGATGACGTGTAATGCCATTTTTTGTTTTTAAAGAAAGTGTCTATGTTTTTCAGCTGCTGTTTCGTTTACCCCATAATATTAACTGTATATGATTTGCTTTTTTAAACATGTTGATAACTGc       >  2:2473385/1‑140 (MQ=255)
                                                                                                                               ggTCGTTTTTTTGATGCTTGAAGACGTGTAAAGCCAATTTTTGTTTTTTAAGAAAGTGTGTATCTTTTGCAGCGGGTGTGTCGGTAACCCCATGATATTCACTGTATATTCTGTTCTTTTGTCAACAGTTTGATAAATGc       >  2:1463179/1‑140 (MQ=255)
                                                                                                                               ggTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                          >  2:7329977/1‑89 (MQ=255)
                                                                                                                               ggTCGTTTTTTTGATGCAGGATGACGTGTAATTGCATTTTTTCTTTTTAAAGAAAGTGTCTATATTTTTAAGCATCTGTTTCTTTAACCCCTTAATATTAACTTTATATGATTTGCTTTTTTAAACATGTTGATAAATga       >  2:5339549/1‑139 (MQ=255)
                                                                                                                                gTCGTGTTTTTGATGCTGGATGGCGGGTAATTCCCTTTTTTCTTTTTTAAGAAAGTGTGTATCTTTTTGAGCTGCTGTTTCGTTAACCCCGTAATATTGACTGTGTATGATGTGCTTTTGTGAAACTGGTTATAAcctgt      >  2:4830417/1‑136 (MQ=255)
                                                                                                                                  cGTTTTTTTTATGCTGGATGACGTGTAATGCCATTTTTTATTTTTAAATAAAGTTTCTATCTTTTGCAGCTGCTGTTTCGTTAAACCCATAATATTAAATGTAAATGATTTTGTTTTTTAAAACTTTTTATaaaatactt    >  2:5061541/1‑133 (MQ=255)
                                                                                                                                   gTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGa   <  2:6618646/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                    
TTTCTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGAT  >  NZ_CP034943/3496245‑3496516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: