Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,498,775 C→A 6.4% Q2K (CAA→AAA)  EO946_RS18005 → spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,498,7750CA 6.4% 982.4 / 4.3 462Q2K (CAA→AAA) EO946_RS18005spore germination protein
Reads supporting (aligned to +/- strand):  ref base C (124/288);  new base A (2/48);  total (126/336)
Fisher's exact test for biased strand distribution p-value = 1.55e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 466 total aligned reads displayed.

GAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACAGA  >  NZ_CP034943/3498638‑3498914
                                                                                                                                         |                                                                                                                                            
     cGCTTCCTTGTTATGATCTCACAGTCAATACAAGGTTTGTTTCAGCCTTTTCCTTATCAATAATAATTCTCCTTTTTCTATACAAATTAAGAAAAACCGTCAATCTTTTTTAGAAAGGGGAGAAAAACTATGCAAATCgc                                                                                                                                       <  2:2196542/140‑2 (MQ=255)
          ccTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTg                                                                                                                                  <  2:6077898/140‑1 (MQ=255)
                  ttatATCACTTTCAATAGACGGTTTGTTTCAGCATTTTCATTATGAAGAATAATTCTCCTTTTTTGATACAAATTAACAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAg                                                                                                                          <  2:6750200/140‑1 (MQ=255)
                  ttatATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAg                                                                                                                          <  2:375782/140‑1 (MQ=255)
                  ttatATAAATTTCAATAGAAGTTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAACGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAg                                                                                                                          <  1:4101875/140‑1 (MQ=255)
                    atatCAGTTTAAATATATGTTTTTTTTCTGCCTTTTCCTTATGAATAATAATTATACTTTTTTGATACAAATTAATAAAAACCTTAATTTTTTTTTATAACGGGTACAGAAAATTTGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  2:624368/140‑1 (MQ=255)
                    atatCACTTTCATTAGAAGTTTTGTTTCAGCCTTTTCCTTATGAATAATAATTCTCATTTTTTTATACAAATTAATAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAAATATGAAAATCGACTCTGATCTCCAGca                                                                                                                        <  1:5315352/140‑1 (MQ=255)
                    atatCACTTTCAATATAAGTTTTTTTTCAGCCTTTTCATTATTAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAAGTATGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  1:7547027/140‑1 (MQ=255)
                    atatCACTTTCAATAGACGGTTTGTTTACGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGca                                                                                                                        <  1:3230611/140‑1 (MQ=255)
                    atatCACTTTCAATAGAAGTTTTGTTTCAGCCTTTTCATTATTAAGAATAATTCTCATTTTTTTATAAAAATTAATAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  1:1366819/140‑1 (MQ=255)
                    atatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAATAATAATTCTCTTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  1:1570706/140‑1 (MQ=255)
                    atatCACTTTCAATAGAAGGTTTGTTTCAGCATTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTAAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  2:4774403/140‑1 (MQ=255)
                    atatCAATTTCAATATAAGGTTTGTTTCAGCCTTTTCCTTATGAATAATAATTCTCATTTTTTGATACAAATTAATAAAAACCGTCAATCTTTTTTATAACGGGGAGATAAAATATGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  1:1245900/140‑1 (MQ=255)
                    atatAACTTTAAATAGAAGTTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAATAAAAACGGTAAATCTGTTTTATAAAGGGTAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                                                                                        <  2:2407029/140‑1 (MQ=255)
                    atacCAATTTCAATAGAAGGTTTTTTTCAGCATTTACCTTATTAAGAATAATTCTCCTTTTTGGAAAAAATTTAAGAAAAACCGTCAATCTTTTTTATAACGGGGAGATAAACTATGCAACTCGACTCTGATCTCCAGca                                                                                                                        <  1:489755/140‑1 (MQ=255)
                     tatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                                                                                                                       <  1:5355245/140‑1 (MQ=255)
                      atCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcaca                                                                                                                      >  2:487249/1‑140 (MQ=255)
                      atCACTTTCAATAGAAGGTTTGTTTCAGACTTTTCCTTATGAAGAATAATTATCCTTTTTTGATAAAAATTAAGAAAAACAGTAAATGTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcaca                                                                                                                      <  1:7326972/140‑1 (MQ=255)
                             tCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGa                                                                                                               >  1:3792442/1‑140 (MQ=255)
                             tCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGa                                                                                                               >  2:6342616/1‑140 (MQ=255)
                             tCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGAACAATATAGa                                                                                                               >  2:139415/1‑140 (MQ=255)
                                    aaGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTg                                                                                                        >  2:2027290/1‑140 (MQ=255)
                                                      ttCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCaa                                                                                     >  1:4557618/1‑140 (MQ=255)
                                                          ttATGAAGAATAATTCTCATTTTTTGATAAAATTTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATg                                                                                 <  2:3653933/140‑1 (MQ=255)
                                                           tATTAATAATTATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAATGGGGATATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGa                                                                                <  1:6198419/140‑1 (MQ=255)
                                                           tATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGa                                                                                <  1:4859460/140‑1 (MQ=255)
                                                           tATCAATAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAAATATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGa                                                                                <  1:2998137/140‑1 (MQ=255)
                                                            aTGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCTACTCTGATATCCAGCACAATATAGAAACATTGAAAAAAAAATTGTGGCAAAATGAc                                                                               >  2:3969784/1‑140 (MQ=255)
                                                             tcaagtaTAATTCTCCTTTTTTGAGACAAATGAAGAAAAACCGTCATTCTGTTTTATCAGGGGGAGAGAAACTATGCAAATCGACGCTGATCTCCAGCACAATCTAGAAACATTGAAAAAAAAATTGGGGCAAAATGACg                                                                              <  2:4336165/138‑1 (MQ=255)
                                                             tcaaaaataatTCTCCTTTTTGTATAAAAATTAACAAAAACCGTAAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACg                                                                              <  1:6688840/135‑1 (MQ=255)
                                                             tGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATATGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACg                                                                              <  1:5787103/140‑1 (MQ=255)
                                                                  aataatTCTCCTTTTTTTATAAAAATTAACAAAAACCGTCAATCTGTTTTATAACGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         <  2:6846920/140‑1 (MQ=255)
                                                                  aataatTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         <  2:1454336/140‑1 (MQ=255)
                                                                  aataatTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         <  2:2770175/140‑1 (MQ=255)
                                                                  aataatTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         >  2:307083/1‑140 (MQ=255)
                                                                  aataatTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         <  2:7097425/140‑1 (MQ=255)
                                                                  aataatACTCCTTTTTTCATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         <  2:2470251/140‑1 (MQ=255)
                                                                  aataatACCCCGTTTTTGATAAAAATAAACAAAACCCGACAATCGGTTTTATAAAGGGGAGACAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATc                                                                         <  1:1438382/140‑1 (MQ=255)
                                                                          tcattttttGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTta                                                                 <  1:4268906/137‑1 (MQ=255)
                                                                            cTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCACAATGACGATATCATAGTTtata                                                               >  1:6566445/1‑140 (MQ=255)
                                                                            cTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTtata                                                               >  1:687496/1‑140 (MQ=255)
                                                                                tttGATACAAATTAACAAAAACCGTCAATCTGTTTTATAAAGGGGATAGAAACTAGCCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtt                                                           <  2:4090673/140‑1 (MQ=255)
                                                                                tttGATAAAAATTAAGAAAAACCGTAAATCTGTTTAATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtt                                                           <  1:3917960/140‑1 (MQ=255)
                                                                                ttggATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGACAGAAACTATGCAAATCGACTCTGATCTCCAGCCCAATATAGACACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtt                                                           <  1:798999/137‑1 (MQ=255)
                                                                                  ttaTACAAATTAAAAAAAACCGTCACTCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtttt                                                         <  2:2502291/138‑1 (MQ=255)
                                                                                  tcaTACAAATTAACAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGtttt                                                         <  2:7381062/138‑1 (MQ=255)
                                                                                    aTACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTTGGGCAAAAAGACGATATCATATATTATACGTTTTCt                                                       >  2:3821259/1‑140 (MQ=255)
                                                                                         aaTTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTgg                                                  <  2:414029/140‑1 (MQ=255)
                                                                                           ttAATAAACCCCGGCAATGTTTTTTATAACGCGGTGACTAATTCTGCAAATCGACTCGGATCTCCAGCCCAATAGAGACACATTGAACAAAATTTTGCGGGAAAATGCCGATCTCATATTTTATACGTTTTCTATTGgag                                                <  2:6630333/140‑1 (MQ=255)
                                                                                           ttAAGAAAAACCGTCAATCTGTTTTATAAAGGGTAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgag                                                <  2:159867/140‑1 (MQ=255)
                                                                                           ttAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgag                                                <  2:5379269/140‑1 (MQ=255)
                                                                                           ttAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgag                                                <  2:444286/140‑1 (MQ=255)
                                                                                           ttAAGAAAAACCGTCAATCTGTTGTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATCTAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgag                                                <  2:179862/140‑1 (MQ=255)
                                                                                            tAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGgaga                                               <  2:6400528/140‑1 (MQ=255)
                                                                                               taaaaaCCGTAAATCTGTTTTATAACGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:1173366/139‑1 (MQ=255)
                                                                                               gAAAAACCTTCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:5303608/140‑1 (MQ=255)
                                                                                               gAAAAACCGTCACGCTGTTTTATAAAGGGGAGAGAAACTCTGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGCGCAAAAGGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  1:23550/140‑1 (MQ=255)
                                                                                               gAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:5957952/140‑1 (MQ=255)
                                                                                               gAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:2852949/140‑1 (MQ=255)
                                                                                               gAAAAACCGTCAATCGTTTTTATAAAGGGGAGAGAAACTATCCAAATCGACTCTGATCTCCAGCACAATATAGAAACCTTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:192286/140‑1 (MQ=255)
                                                                                               caaaaaCCGACAAGCTGTTTTATAACGGGGAGAGAAACTAGGCAAATCGACTCGGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:6524017/139‑1 (MQ=255)
                                                                                                aaaaaCCTTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGAcag                                            <  2:4831533/140‑1 (MQ=255)
                                                                                                     ccGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAAGGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGcagaca                                      <  1:2072806/140‑1 (MQ=255)
                                                                                                     ccGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGcagaca                                      <  2:3337977/140‑1 (MQ=255)
                                                                                                     ccGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGcagaca                                      <  1:3350874/140‑1 (MQ=255)
                                                                                                     ccGTAAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGcagaca                                      <  2:5159941/140‑1 (MQ=255)
                                                                                                       gTCAAGCTGTTTTTTAACGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGtagacaga                                    <  1:3659307/140‑1 (MQ=255)
                                                                                                          aattttttttATAAAGGGGAGAGAAAATATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                                 <  2:7602427/134‑1 (MQ=255)
                                                                                                          aaTCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATAGAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                                 <  2:2278370/140‑1 (MQ=255)
                                                                                                          aaTCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                                 <  1:1107496/140‑1 (MQ=255)
                                                                                                           aTCTTTTTTTTAAAGTGGAGATAACCTATTCAAATCGACTCTGATCTCCAGCACAATATACAAACATTGAAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAggg                                 <  2:3446261/140‑1 (MQ=255)
                                                                                                           aTCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGc                                <  1:5461486/140‑1 (MQ=255)
                                                                                                             cttttttATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGt                              <  2:87426/137‑1 (MQ=255)
                                                                                                             cTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGt                              <  2:1078907/140‑1 (MQ=255)
                                                                                                               gTTTTATTACGGCGAGAGCAACTCTCAAACTCGACTCTGATCTCCAGCCCAATAAAGCAACAGTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTAATTTTGGAGACAGCAGACAGAGGGCGTGc                            <  1:6044647/140‑1 (MQ=255)
                                                                                                               gTTTTATAATGGGGCGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  1:1903016/140‑1 (MQ=255)
                                                                                                               gTTTTATAATGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAACATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  1:3446301/140‑1 (MQ=255)
                                                                                                               gTTTTATAAAGGGGAGCGAAACTAGGCAAAGCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  2:5859130/140‑1 (MQ=255)
                                                                                                               gTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  1:7404111/140‑1 (MQ=255)
                                                                                                               gTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  2:3622335/140‑1 (MQ=255)
                                                                                                               gTTTTATAAAGGGGAGAGAAACTATCCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  2:2938869/140‑1 (MQ=255)
                                                                                                               gTTTTATAAAGGGGACAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAAGGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGc                            <  2:6874617/140‑1 (MQ=255)
                                                                                                                  ttATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTACAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtg                         <  2:5699824/140‑1 (MQ=255)
                                                                                                                    ataAAGGGGAGATAAAATATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtgtt                       <  2:5552172/140‑1 (MQ=255)
                                                                                                                    ataAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGACACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtgtt                       <  1:5188016/140‑1 (MQ=255)
                                                                                                                    ataAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtgtt                       <  1:2855513/140‑1 (MQ=255)
                                                                                                                    ataAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtgtt                       <  1:7165760/140‑1 (MQ=255)
                                                                                                                          gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTatat                 >  1:2707804/1‑140 (MQ=255)
                                                                                                                          gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTatat                 >  2:4255023/1‑140 (MQ=255)
                                                                                                                          gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTatat                 >  1:1858865/1‑140 (MQ=255)
                                                                                                                          gggAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCGGTTGTatat                 >  1:7036735/1‑140 (MQ=255)
                                                                                                                           ggAGACAACCTATGCAAATCCACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAACTGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATAtt                <  1:5005714/140‑1 (MQ=255)
                                                                                                                             agagaAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTg               >  1:2281930/1‑140 (MQ=255)
                                                                                                                               agaAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCtgttg                      <  2:6953793/130‑1 (MQ=255)
                                                                                                                                    cTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTg       >  1:6348527/1‑140 (MQ=255)
                                                                                                                                    cTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTg       >  2:2667263/1‑140 (MQ=255)
                                                                                                                                      aTGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATCTTGACGGTTTGAc     >  2:4597373/1‑140 (MQ=255)
                                                                                                                                      aTGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGAc     >  2:3552120/1‑140 (MQ=255)
                                                                                                                                       tGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACa    >  1:5711516/1‑140 (MQ=255)
                                                                                                                                         cAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACAGa  >  1:2484572/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATCATATTTTATACGTTTTCTTTTGGAGACAGCAGACAGAGGGCGTGCCTGTTGTATATTGACGGTTTGACAGA  >  NZ_CP034943/3498638‑3498914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: