Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,511,851 T→G 7.0% Y156D (TAT→GAT)  EO946_RS18060 → LacI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,511,8510TG 7.0% 1504.2 / 16.8 522Y156D (TAT→GAT) EO946_RS18060LacI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (169/290);  new base G (60/2);  total (230/292)
Fisher's exact test for biased strand distribution p-value = 2.04e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 543 total aligned reads displayed.

AACCTTTAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTACAGCAGGCTGAAGC  >  NZ_CP034943/3511712‑3511990
                                                                                                                                           |                                                                                                                                           
     ttAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCTGCATGAATCACCCAGTCGTGTTTCTGGAACCAACACCTGAAGGCGCACCTTCAGTGTCCAGTGACGGCtatata                                                                                                                                        >  1:4339127/1‑140 (MQ=255)
     ttAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACCGCgagata                                                                                                                                        >  2:7676502/1‑140 (MQ=255)
     ttAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTATGGACAGAACACCTGAAGGCGCTCCGTCTTTGTGCAGTGACGGCtatata                                                                                                                                        >  1:722066/1‑140 (MQ=255)
      tAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATag                                                                                                                                       >  2:3601756/1‑140 (MQ=255)
      tAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTGTTTATTGTCAGAAAACCTGAAGGCGCTCCTTCTGTGTCCATTGAAGGCgatatgg                                                                                                                                       >  1:1773217/1‑138 (MQ=255)
         gCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATaggag                                                                                                                                    <  1:6902007/140‑1 (MQ=255)
             aaTCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTGTTTGTGGCCCGGAAACCAGAAGGCGCTCCTTCTGTGTGCCGTGACGGGGAGATAGGTGGgaa                                                                                                                                >  1:687953/1‑140 (MQ=255)
                  tGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTGTGGACACAACACCTCAAGCCGCTCCTACTGGGTCCAGTGACGGCGCGATAGGAGGCAAAGTAg                                                                                                                           >  2:4130940/1‑140 (MQ=255)
                    ttGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGCCAGAACACCGGAAGGCGCTCCGTCTGTGTCCAGTGACGGGGATATAGGAGGAAAATTAgcc                                                                                                                         >  1:5466879/1‑140 (MQ=255)
                           cATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAg                                                                                                                  <  1:3538163/140‑1 (MQ=255)
                           cATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAg                                                                                                                  <  1:7022036/140‑1 (MQ=255)
                              tattGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCca                                                                                                               <  2:5621837/140‑1 (MQ=255)
                               attGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTATTGTGGCCACAACACCAGAACGCGCTCCGTAGGGGTCCACTGACGGCTATATAAGTGGCAAAATAGcccccgcacgccc                                                                                                              >  1:5130217/1‑133 (MQ=255)
                                   ccGCAACGAATTACCCGGATCTTGAGGACTATAGCGGCATGAATCACCCAGTCGTTTTTATGGACAGAACACCAGGAGGCGCTCCGTCTGTGTCCAGTGGCGGGTAGATAGGCGGAAAATTTGCCGCCGGAGCcagaatt                                                                                                          >  2:1823252/1‑135 (MQ=255)
                                    cGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTc                                                                                                         <  2:6992844/140‑1 (MQ=255)
                                     gCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCa                                                                                                        <  1:5390247/140‑1 (MQ=255)
                                     gCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCACTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCa                                                                                                        <  2:862302/140‑1 (MQ=255)
                                                  cGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCcgg                                                                                           <  1:7158832/140‑1 (MQ=255)
                                                     aTCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTATGGACAGAACAACAGAAGGCGCGCCGTATTTGGACAGAGTCGGCTATATAGGCAGAAAAATAGCCGCCGAAACCCACAATCACGGTAAAAGCccgcgg                                                                                        >  2:3096011/1‑140 (MQ=255)
                                                      tCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGa                                                                                       <  1:4114786/140‑1 (MQ=255)
                                                         tGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCa                                                                                    <  2:2064017/140‑1 (MQ=255)
                                                         tGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCa                                                                                    <  2:2284855/140‑1 (MQ=255)
                                                         tGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCa                                                                                    <  2:3362212/140‑1 (MQ=255)
                                                         tGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCa                                                                                    <  1:1457495/140‑1 (MQ=255)
                                                         tGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCa                                                                                    <  2:7414043/140‑1 (MQ=255)
                                                           aGGAATATCGCGGCAGGAATCACCCAGGCGTGTTTCTGGACAGAACACCTGAAGGCGCTCCGTCTGTCTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:6763891/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:2457661/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  2:6048501/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:3343570/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:3624113/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:1687453/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCGGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCAGTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:6435661/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCATGAATCACCCAGTAGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:4731481/140‑1 (MQ=255)
                                                           aGGAATATAGCGGCAGGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  2:1880972/140‑1 (MQ=255)
                                                           aGGAATATAGCCGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCATTCTGTGTCCAGTGACGGATATATAGGAGGAAAATTATCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  <  1:6131531/140‑1 (MQ=255)
                                                                   aGCGGCATGACTCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  1:3081389/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGCACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  1:1263226/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:6535369/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  1:3804550/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:1377212/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:2472471/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:3675929/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  1:3231795/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:4489911/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:2744708/140‑1 (MQ=255)
                                                                   aGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:6813396/140‑1 (MQ=255)
                                                                   aGCGGCATGAACCACCCAGTCGTTTGTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:5185023/140‑1 (MQ=255)
                                                                   aGCCGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTgag                                                                          <  2:2159924/140‑1 (MQ=255)
                                                                      ggCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAgg                                                                       <  2:163977/140‑1 (MQ=255)
                                                                       gCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCGTCTTTGTGCAGTGAAGGGGATATAGGAGGAAAAATAGCCCCCGAAGCCCTCATTCACGGTAACAGCCGGCGGATAACGCACTTGAGAggg                                                                      >  2:5842712/1‑139 (MQ=255)
                                                                       gCATGAATCACCCAGTCGTGTTTCTGGACAGAACACCAGAAGGCGCGCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGt                                                                      >  2:3433606/1‑140 (MQ=255)
                                                                        gagacagcACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAACAGCCGGCGGATCACGCTCTTGAGAGGTc                                                                     <  1:2237029/133‑1 (MQ=255)
                                                                        cATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTc                                                                     <  1:2386176/140‑1 (MQ=255)
                                                                          tGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCGATATAGGAGGAAAATTATCCGCCGAACCCATCATTCACGGTAAAAGCCCGCGGATCACGCTGTTGTGAGGTCCt                                                                   >  2:7212402/1‑140 (MQ=255)
                                                                          tGAATCACCCAGTCGTGTTTGTGGACAGAACACCTGAAGGCGCTCCTTGGGTTTCCAGTGTCGGCTATATAGGGGGTAAATTAAGCGCCGAATCCATCAATCAAGGGAAAATCCGGCGGTTCACGCTGGTgagttggact                                                                   >  1:4790494/1‑133 (MQ=255)
                                                                             aTCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGACGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCt                                                                <  2:4001476/140‑1 (MQ=255)
                                                                             aTCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCt                                                                <  2:6714789/140‑1 (MQ=255)
                                                                             aTCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCGATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCt                                                                <  1:4490910/140‑1 (MQ=255)
                                                                             aTCACCCAGTCGTTTTTATGGACAGAACACCAGAAGGCGCTCCTTCTGTGTCCAGTGAAGGCTATATATGAGGAAAATTAGCCGCCGAAGCCAACATTCACGGTAAAAGCCGGCGGATAAAGCTCTTTACAGGGCCGGca                                                                >  2:5918945/1‑139 (MQ=255)
                                                                               cACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCACCTTCTGTGTCCAGTGTCGGGTATATAGGAGGAAAATTAGCCGCCGAAGCCCTCATTCACGGTAAAAGCCCGCGGATCACGCTCTTGATAGGTCCTGCTCa                                                              >  2:5484931/1‑140 (MQ=255)
                                                                                aCCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGTAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCAc                                                             <  2:4339602/140‑1 (MQ=255)
                                                                                aCCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCAc                                                             <  1:3937904/140‑1 (MQ=255)
                                                                                     gTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGATGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGcc                                                        <  1:5261023/140‑1 (MQ=255)
                                                                                     gTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGcc                                                        <  1:4626864/140‑1 (MQ=255)
                                                                                       cgctgttCTGGACAGAACACCTGAAGGCGCTCCTACTCTGTCCAGTGACGGATATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAACGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGccga                                                      <  1:7631607/135‑3 (MQ=255)
                                                                                       cGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTa                                                      <  1:2034258/140‑1 (MQ=255)
                                                                                       cGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTa                                                      <  1:6691445/140‑1 (MQ=255)
                                                                                       cGTTTGTCTGGACAGAACACCTGAAGGCGCTCCGTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTa                                                      <  1:5782823/140‑1 (MQ=255)
                                                                                        gTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTAc                                                     <  1:5603699/140‑1 (MQ=255)
                                                                                                ggACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAg                                             >  2:6880867/1‑140 (MQ=255)
                                                                                                ggACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAg                                             >  2:4633341/1‑140 (MQ=255)
                                                                                                  aCAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGAc                                           <  1:803977/140‑1 (MQ=255)
                                                                                                      aaCACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCt                                       >  2:4829479/1‑140 (MQ=255)
                                                                                                      aaCACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCt                                       >  1:2848007/1‑140 (MQ=255)
                                                                                                      aaCACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTAACGGTAAAAGCCGGCGGATCACGCTCGTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCt                                       >  2:3719638/1‑140 (MQ=255)
                                                                                                           cTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAAc                                  >  1:1339601/1‑140 (MQ=255)
                                                                                                               aGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACTCTCTTGAGAGGTCCTGCTCACCTGCCTACCGATCAACACCGCGTTAACGgcg                              >  1:155348/1‑140 (MQ=255)
                                                                                                               aGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGgcg                              <  1:4160575/140‑1 (MQ=255)
                                                                                                                ggCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGACAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGgcgc                             >  2:153621/1‑140 (MQ=255)
                                                                                                                ggCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGgcgc                             >  1:966133/1‑140 (MQ=255)
                                                                                                                ggCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGgcgc                             >  2:2562223/1‑140 (MQ=255)
                                                                                                                   gcTCCTTCTGTGTCCATTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCttt                          <  2:6249509/140‑1 (MQ=255)
                                                                                                                     tCCTTCTGTGTCCAGTGACGGCTATATAGGAGGACAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTGTAACGGCGCTTTgg                        >  2:3239615/1‑140 (MQ=255)
                                                                                                                     tCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACg                                                                                  >  1:5907294/1‑82 (MQ=255)
                                                                                                                     tCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCt                                                                >  2:4697779/1‑100 (MQ=255)
                                                                                                                     tCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCaa                                              >  1:1152735/1‑118 (MQ=255)
                                                                                                                     tCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCaa                                              <  2:1152735/118‑1 (MQ=255)
                                                                                                                     tCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTgg                        >  1:6032368/1‑140 (MQ=255)
                                                                                                                      ccTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCCCGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGga                       <  2:1714210/140‑1 (MQ=255)
                                                                                                                      ccTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGga                       <  2:2872454/140‑1 (MQ=255)
                                                                                                                           tgtgTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCt                  <  1:7391990/140‑1 (MQ=255)
                                                                                                                               tCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTAca              >  1:7582194/1‑140 (MQ=255)
                                                                                                                               tCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTAca              >  2:4257957/1‑140 (MQ=255)
                                                                                                                                 cAGTGACTGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTAACGGTAAAATCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACAGCTCAATACCACTTTAACGGCGCTTTGGAGATCTTAaagc            >  2:476355/1‑140 (MQ=255)
                                                                                                                                 cAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTAcagc            >  2:3900581/1‑140 (MQ=255)
                                                                                                                                    tGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGga                       <  1:1046813/126‑1 (MQ=255)
                                                                                                                                    tGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGga                       <  1:4890455/126‑1 (MQ=255)
                                                                                                                                    tGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTACAGCAgg         >  1:2949061/1‑140 (MQ=255)
                                                                                                                                       cGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTACAGCAGGCTg      >  2:7102082/1‑140 (MQ=255)
                                                                                                                                         gCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGga                       <  2:6379233/121‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AACCTTTAAGCAAAATCATGTTGCAGGCATTATTGCCGCAACGAATTACCCGGATCTTGAGGAATATAGCGGCATGAATCACCCAGTCGTTTTTCTGGACAGAACACCTGAAGGCGCTCCTTCTGTGTCCAGTGACGGCTATATAGGAGGAAAATTAGCCGCCGAAGCCATCATTCACGGTAAAAGCCGGCGGATCACGCTCTTGAGAGGTCCTGCTCACCTGCCTACCGCTCAAGACCGCTTTAACGGCGCTTTGGAGATCTTACAGCAGGCTGAAGC  >  NZ_CP034943/3511712‑3511990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: