Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,539,642 A→C 5.1% L104R (CTG→CGG)  EO946_RS18215 ← T7SS effector LXG polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,539,6420AC 5.1% 1221.4 / 6.1 421L104R (CTG→CGG) EO946_RS18215T7SS effector LXG polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (152/225);  new base C (38/2);  total (194/227)
Fisher's exact test for biased strand distribution p-value = 3.31e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

CATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGTCCC  >  NZ_CP034943/3539505‑3539779
                                                                                                                                         |                                                                                                                                         
   cGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCa                                                                                                                                      <  2:4606337/140‑1 (MQ=255)
   cGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCa                                                                                                                                      <  2:4530260/140‑1 (MQ=255)
   cGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAAAAATTCTGATGCTTTCAAGTATCCATTGGGCAGCTCa                                                                                                                                      >  2:3174315/1‑140 (MQ=255)
        aTCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATc                                                                                                                                 <  2:3021647/140‑1 (MQ=255)
             aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCaaaa                                                                                                                            <  1:5699133/140‑1 (MQ=255)
             aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGGTTTTGAGCGGAGACCAATTATGAGGCTTTGCAGTCGCCAATTGGCACCTCAAGATACaaaa                                                                                                                            >  1:2221468/1‑140 (MQ=255)
                cccATAGTCGTCAAGCCTGTACCTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaag                                                                                                                         <  1:3880393/138‑1 (MQ=255)
                    tAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGt                                                                                                                     >  2:5677356/1‑140 (MQ=255)
                      gtcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGttt                                                                                                                   <  2:285501/140‑1 (MQ=255)
                       tcgtcAAGCGTGTACCTGTCGAGATCAATAATATCGCTGAGGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                                                                  <  2:6124392/140‑1 (MQ=255)
                       tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                                                                  <  1:2308270/140‑1 (MQ=255)
                       tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTAATGATCCAAAAAAGAAGTTTc                                                                                                                  <  1:6973260/140‑1 (MQ=255)
                       tcgtcAAGCGTGTACATGTCGAGATCAATAAGATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                                                                  <  2:1287763/140‑1 (MQ=255)
                        cgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTcc                                                                                                                 <  1:5450802/140‑1 (MQ=255)
                         gtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGAGCCAAATTATGAGGCGTTCAAGTCGCCATTGGGCAGGGAATGATCCAAAAAAGAAGTTTGca                                                                                                                >  2:844273/1‑140 (MQ=255)
                         gtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCca                                                                                                                <  2:1570834/140‑1 (MQ=255)
                            aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATTATCGGGGAAAAATTATGAGGCGTTCAATTCTCCATTGGGGAGCTCATTATCCAAAAAAGAAGTTTAcacaa                                                                                                             >  1:1239430/1‑139 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGATCGGAGACAATTTGTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATGAAAAAAAGAATTTTACAAAAATGaa                                                                                                        >  1:6520042/1‑140 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGCGACAATTTCTGAGGCTTTCAAGTGTCCATTGGGCAGCTCATTATCCAAAAAAGAAGTTTCCACataataa                                                                                                        >  1:5273072/1‑136 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGCGACAAATTCTTGGGCTTTGAAGTCGCCATTTGGCAGCGCATTTTCACAAAAAGAAAGTTACACAAATGaa                                                                                                        >  1:502930/1‑140 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAAATTCTGAGGCTTTAAAGTCTCCATTGGGCAGCTCATTATCCAAAAAAGTAATTTACACATATgta                                                                                                        >  1:3663279/1‑138 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                                                                        >  2:3610021/1‑140 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                                                                        <  2:7667556/140‑1 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATGTCTTCGTTTTGTGCGGGGACACTTTGTGAGGCATTGAAGGCGACCTTGGGGCGCGCATTGTCCAAAAAAGAACGTTCCAACTATgtc                                                                                                        >  1:1421162/1‑138 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATGTGCGGTGACAAGTTGTGATGCTTTAAACGGTCCATTTGGCCGCTCATGATCCCAAAAATAATTTTGCACATATGaa                                                                                                        >  1:1666746/1‑140 (MQ=255)
                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGGTCTTTATTAGCGGAGACAAATTATGAGGCTTTGAAGTCTCCCTTTGGCAGCTCAAGATACAAAAAAGAAGTTTACACCAATGaa                                                                                                        >  1:5881182/1‑140 (MQ=255)
                                      aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAtt                                                                                                   <  1:329697/140‑1 (MQ=255)
                                       tGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTCTTGTGTATGAGCGGCGACCATTTGTGAGTCTTTGAATTCTCCATTGGGGAGCTAATGTTCCAAAAAAGAAGTTTGCACATATGAAtaaatc                                                                                                  >  1:7146780/1‑135 (MQ=255)
                                        gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTGTGAGGCTTTCAAGGCTCCATTGGGCAGCGCATGATCCAAAAAAGAAGTTTGCACATATGAATCAttga                                                                                                 >  2:551358/1‑138 (MQ=255)
                                        gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAATTTTCCACATATGaaaaaataa                                                                                                 >  2:7314264/1‑133 (MQ=255)
                                        gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATTAATAATTCa                                                                                                 >  2:2817204/1‑140 (MQ=255)
                                         tCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACat                                                                                                             >  2:1102489/1‑127 (MQ=255)
                                           gagaTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGGTCGTTATGAGCGGGCACAATTTCTGAGGCTTTGAAGTCTCCATTGGGCCGCTCATTATCCCAAAAAAAAATTTCCACAAATGAATGATTCAGtt                                                                                              >  1:735178/1‑140 (MQ=255)
                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                           <  1:1549033/140‑1 (MQ=255)
                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                           <  1:258773/140‑1 (MQ=255)
                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                           <  2:4347404/140‑1 (MQ=255)
                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                           <  1:6654708/140‑1 (MQ=255)
                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                           <  2:6597407/140‑1 (MQ=255)
                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                                                                           >  2:1273595/1‑140 (MQ=255)
                                               tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCTAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                                                                          <  2:1203389/140‑1 (MQ=255)
                                                cAATAATATCGCTGATGCATGACAAAATGGAGTCGATTTCTTCTTTATGAGCGTATACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTAATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGa                                                                                         <  2:6267092/140‑1 (MQ=255)
                                                  ataataTCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAAATTCTGAGGCTTTCAAGTCTCCATTGGGCCGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATAATTAAGTTCTTGAta                                                                                       >  2:1742109/1‑139 (MQ=255)
                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGGCGCTCATGATCCAAAAAAGAATTTTACACATATGAATAATTCAGTTCTTGATCTTTAAt                                                                                 >  2:7034887/1‑140 (MQ=255)
                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                                                                 <  2:6762103/140‑1 (MQ=255)
                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                                                                 <  2:1860693/140‑1 (MQ=255)
                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                                                                 <  1:691358/140‑1 (MQ=255)
                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                                                                 <  1:5837824/140‑1 (MQ=255)
                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                                                                 <  1:4969506/140‑1 (MQ=255)
                                                          gCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGt                                                                               <  1:427157/140‑1 (MQ=255)
                                                          gCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGt                                                                               <  2:1460872/140‑1 (MQ=255)
                                                           cTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCGTTCAAGTCTCCATTGGGGCGCTCATGATCCAAAAAAGAAGTTTCCACAAATTAATCATTGAGTTCTTTATATTTAAttta                                                                              >  2:3887144/1‑137 (MQ=255)
                                                             gATGCCTGACAAAATGGAGTCGATGTGTTCTTTATGATCGGAGACACTTTATGAGGCTTTGAAGTGTCCATTGGGCAGCGCATGATGCAAAAAAGAAGTTTGCACATATGGATAATTGAGTTCTTGATCTTTAATGtctc                                                                            >  1:4420131/1‑140 (MQ=255)
                                                                   tGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGCGACCATTTCTGAGGCGTTCAAGTGTCCATTGGGCAGCTCATGATCCAAAAAAGTAGTTTCCACCTATGAATCATTGAGTTCTTGATATTTTATGTGTCCTGcaa                                                                      >  2:4744837/1‑140 (MQ=255)
                                                                   tGACAAAATGGAGTCGATTTATTGTTTATGAGCGGAGACAAATTGTGGGGCTTTGAAGTCTCCATTGGGCCGCAGATGATGCAAAAAAGAAGTTTACACAAATTTATCATTCAGTTGTTGATGTTTTATGTTTGCTTaaa                                                                      >  2:2467418/1‑140 (MQ=255)
                                                                     aCAAAATTGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATg                                                                    <  2:1967771/140‑1 (MQ=255)
                                                                     aCAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAAATTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCAAGATCCAAAAAAGAAGTTTCCACATATTAATCATTCAGTTCTTGATATTTAATTTCTCCTGAAAtt                                                                    >  2:5781106/1‑139 (MQ=255)
                                                                           tGGAGTCGATTTATTCTTTATTAGCGGAGACAAATTCTGAGGCTTTCAAGTCTCCAATGGGCAGCTCATGATCCAAAAAAGAAGTTTACACATATTAATCATTCAGTTATTGATCTTTAATTTATACAGAAATGACCttt                                                              >  2:5964989/1‑140 (MQ=255)
                                                                                 cGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAAc                                                        <  1:915692/140‑1 (MQ=255)
                                                                                    tttCTTGTTTATGAGCGGAGACAAATTCTGATGCTTTAAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAATTTTACACATATGAATCATTCAGTTATTGATCTTTAATGTGTCCGGAAAAGACATTTAAAAACGCa                                                     >  2:4126416/1‑140 (MQ=255)
                                                                                            ttctGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATCTGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGc                                             <  1:6265662/137‑1 (MQ=255)
                                                                                            ttctGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGc                                             <  2:5063622/137‑1 (MQ=255)
                                                                                            ttATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGc                                             <  1:3031217/140‑1 (MQ=255)
                                                                                            ttATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGc                                             <  2:517125/140‑1 (MQ=255)
                                                                                             tATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCCAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCa                                            <  1:1839745/140‑1 (MQ=255)
                                                                                             tATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCa                                            <  2:397556/140‑1 (MQ=255)
                                                                                             tATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCa                                            <  2:7200220/140‑1 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTACTGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       <  2:7523582/140‑1 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       >  1:1958321/1‑140 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       <  2:4791742/140‑1 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       <  1:3174315/140‑1 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       >  2:2738338/1‑140 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       <  2:2071515/140‑1 (MQ=255)
                                                                                                  gCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTACACAAATGAATCATTCAGTGCGTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGATAc                                       >  2:497379/1‑140 (MQ=255)
                                                                                                  gCGGAGACAATGTCTGATGCTTTCAACTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATCTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGAc                                       <  1:4858379/140‑1 (MQ=255)
                                                                                                     gagaCAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAg                                    >  1:6814508/1‑140 (MQ=255)
                                                                                                        aCAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAgtgt                                 >  1:3457053/1‑140 (MQ=255)
                                                                                                             ttCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCACTTTGAGCAGAGACAAGTGTcagcc                            >  1:2138298/1‑140 (MQ=255)
                                                                                                                   ggCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCttt                      >  1:1064584/1‑140 (MQ=255)
                                                                                                                      tttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                   >  2:4393285/1‑140 (MQ=255)
                                                                                                                      tttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                   <  2:536117/140‑1 (MQ=255)
                                                                                                                      tttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                   <  2:1613322/140‑1 (MQ=255)
                                                                                                                      tttCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                   <  2:6428990/140‑1 (MQ=255)
                                                                                                                      tttCAAGGCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcga                   <  1:4053265/140‑1 (MQ=255)
                                                                                                                        tCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTcgacg                 >  1:7680444/1‑140 (MQ=255)
                                                                                                                          aaGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGAt               <  1:5577862/140‑1 (MQ=255)
                                                                                                                           aGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGAtt              <  1:614754/140‑1 (MQ=255)
                                                                                                                           aGTCTCCATTGGGCACCTCATGATCCAAAAAAGAAGTTTCCACATATGACTCATTCAGTTCGTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCGTTCGACGAtt              <  1:4750619/140‑1 (MQ=255)
                                                                                                                            gTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGAttt             <  1:2691536/140‑1 (MQ=255)
                                                                                                                               tcCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCg          >  2:334633/1‑140 (MQ=255)
                                                                                                                               tcCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCg          >  1:6062209/1‑140 (MQ=255)
                                                                                                                               tcCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCg          >  1:840117/1‑140 (MQ=255)
                                                                                                                                ccATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGc         <  1:2818350/140‑1 (MQ=255)
                                                                                                                                ccATTGGGCAGCACATGATCCCAACAAGAAGTTTCCACATCTGAATCATTCACTTCTGCATCTTTAATGTCACCTGAAATGCCCTTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGc         <  2:3810575/140‑1 (MQ=255)
                                                                                                                                 cATTGGGCAGCTCATGATCCAACAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACACGTGTCAGCCAGCTTTCGACGATTTCCGcc        >  2:5416579/1‑140 (MQ=255)
                                                                                                                                  aTTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCt       <  2:6062209/140‑1 (MQ=255)
                                                                                                                                  aTTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCt       <  2:3754089/140‑1 (MQ=255)
                                                                                                                                     gggCCGCTCATGATACAAAAAAGAAGTTTCCACATACGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCAATTAAAAACGCACTTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGTc    <  1:3757219/140‑1 (MQ=255)
                                                                                                                                      ggCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGTcc   <  2:2278924/140‑1 (MQ=255)
                                                                                                                                       gCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGTccc  >  1:6712853/1‑140 (MQ=255)
                                                                                                                                       gCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGTccc  >  1:4716567/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAATTTGAGCAGAGACAAGTGTCAGCCAGCTTTCGACGATTTCCGCCTGTCCC  >  NZ_CP034943/3539505‑3539779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: