Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,561,985 G→C 15.0% intergenic (‑631/‑167) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,561,9850GC15.0% 957.7 / 47.6 401intergenic (‑631/‑167)EO946_RS18300/EO946_RS21105response regulator/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (171/157);  new base C (2/66);  total (175/226)
Fisher's exact test for biased strand distribution p-value = 2.24e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

ATCAGATCAAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGA  >  NZ_CP034943/3561846‑3562118
                                                                                                                                           |                                                                                                                                      
atcatatcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCTTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAg                                                                                                                                        <  1:5123559/140‑1 (MQ=255)
 tcagatcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATATGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGa                                                                                                                                       <  1:522322/140‑1 (MQ=255)
 tcagatcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGa                                                                                                                                       <  2:7136932/140‑1 (MQ=255)
  cagatcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGAc                                                                                                                                      <  2:3564997/140‑1 (MQ=255)
  cagataaAAGCAAGAAAATCAGCTTTTCGCTATCATCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATTAACCTGATTTTATAGCGCAAAAAAGAc                                                                                                                                      <  2:4427629/140‑1 (MQ=255)
    gatcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAAACTGATTTTATATCGCAAAACAGACCa                                                                                                                                    >  1:739222/1‑140 (MQ=255)
     atcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCACATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGAGTTTATAGCGCAAAACAGACCat                                                                                                                                   >  2:5206056/1‑140 (MQ=255)
     atcaAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCat                                                                                                                                   >  2:6120216/1‑140 (MQ=255)
         aaGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATAc                                                                                                                               <  1:5552666/140‑1 (MQ=255)
          aGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATATCGCAAAACAGACCATATACg                                                                                                                              >  2:6925820/1‑140 (MQ=255)
          aGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGa                                                                                                                                       >  1:1251819/1‑131 (MQ=255)
          aGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGCAAGCCCTTCAAATAAACCTGATTTTATAGAGCAAAACAGACCCTATACg                                                                                                                              >  2:4741714/1‑140 (MQ=255)
            cAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAg                                                                                                                            <  1:2815565/140‑1 (MQ=255)
               gACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCtt                                                                                                                         >  2:5395279/1‑140 (MQ=255)
               gACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATATATTTTAATGGCCAAGTAATAGGACAAAGCGGAAGCCCTTAAAATAAACCTGATTTTATATCGCAAAACAGAACATATACGAGCtt                                                                                                                         >  1:4304076/1‑140 (MQ=255)
                    tCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTa                                                                                                                    <  1:6359430/140‑1 (MQ=255)
                    tCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTa                                                                                                                    <  2:2571473/140‑1 (MQ=255)
                      aGGTTTTCTGGATCAGAACGTCGTTTATTTAAAGTGCAAATCGCATCTATTTTAATGGACAATTAATAGGACAAATCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACg                                                                                                                  <  1:7232944/140‑1 (MQ=255)
                      aGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACg                                                                                                                  <  1:7122600/140‑1 (MQ=255)
                      aGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACg                                                                                                                  <  2:3903766/140‑1 (MQ=255)
                      aGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACg                                                                                                                  <  1:1896210/140‑1 (MQ=255)
                           ttCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCGTca                                                                                                             >  1:6186541/1‑140 (MQ=255)
                             cGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGCCCATATACGAGCTTTTTTACGCTTcaca                                                                                                           <  1:990732/140‑1 (MQ=255)
                             cGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTcaca                                                                                                           >  2:1654631/1‑140 (MQ=255)
                                 aTCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCCTAGACGAGCTTTTTTACGCTTCACAAggg                                                                                                       <  2:5520696/140‑1 (MQ=255)
                                       aCGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACaa                                                                                                 <  1:6888754/140‑1 (MQ=255)
                                          tcgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGa                                                                                             >  1:4839036/1‑140 (MQ=255)
                                               tatttaCAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTtg                                                                                        <  1:3324019/140‑1 (MQ=255)
                                                       gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                >  2:2171524/1‑140 (MQ=255)
                                                       gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                >  1:3809148/1‑140 (MQ=255)
                                                        tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACg                                                                                                                  >  1:670882/1‑106 (MQ=255)
                                                        tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                               >  1:1341832/1‑140 (MQ=255)
                                                        tGCAAATCGCATCTATTTTAATGGCCAATGAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                               >  1:5493089/1‑140 (MQ=255)
                                                                  aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACATACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                     >  2:1860114/1‑140 (MQ=255)
                                                                  aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                     >  1:918954/1‑140 (MQ=255)
                                                                  aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                     >  2:4606321/1‑140 (MQ=255)
                                                                              ggCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGcc                                                         >  2:5069714/1‑140 (MQ=255)
                                                                               gCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCg                                                        >  2:28473/1‑140 (MQ=255)
                                                                                       aTAGGACACAGCGGAAGCCCTACAAATAAACCTTATTTTATAGCGCAAAACACACCATATACGAGCTTTTTTCCGCTTCACACGGGTGACAATGAACTTGTGGTAAAATTTACAAACTTCGTAAAAACGCCGGGGTgggg                                                <  1:3442667/140‑1 (MQ=255)
                                                                                       aTAGGACAAAGCGCAAGCCATTCAAATAAACCTGATTTTATAGCGCAAAACACACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTgggg                                                <  2:792933/140‑1 (MQ=255)
                                                                                       aTAGCACAAAGCGGAAGCCCTTAAAATAAACCTTATTTTATCGCCCAAAACACACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGTAAAAACGCCGGGGTgggg                                                <  1:6582994/140‑1 (MQ=255)
                                                                                         aGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGaa                                              >  1:5368923/1‑140 (MQ=255)
                                                                                              aacgCGGACGCCCTTAAAATAACCCTGATTTTATAGCGCAAACCCCCCCATATACGCGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                         <  2:6275454/137‑1 (MQ=255)
                                                                                              aaaGCGCAAGCCATACAAAAAAACCTTTTTTTATAGCGAAAAACAGACCATATAGTAGCTTTTTTACGCTTCAAACGGGTCAAAATTAACTTGGGATAAACTTTACAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCa                                         <  1:5775380/140‑1 (MQ=255)
                                                                                               aaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaa                                        >  1:4764032/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGTTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGTCAATGAACTTGTGGTACAATTTTCAAACTTCGGAAATTCGCCGGGGTGGGGAATGGCaac                                       >  1:5620737/1‑139 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGACTCGCaaa                                       >  1:3120457/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                       >  2:6003366/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                       >  2:2461801/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                       >  1:2155783/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                       >  1:6032534/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTGTTTTACGCATCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                       >  1:705046/1‑140 (MQ=255)
                                                                                                aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATAGACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGATAGACGCCGGGGTGGGGAAGCGCaag                                       >  1:3995983/1‑139 (MQ=255)
                                                                                                    gAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCg                                   >  1:4157143/1‑140 (MQ=255)
                                                                                                     aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                       <  2:4873207/72‑1 (MQ=255)
                                                                                                      accccTTAAAATAAACCTGATTTTATAGCGAAAACCAGCCAATAAACGAGGTTTTTAACGGTTCCAACGGGTGACAATCAACGTGTGGTACAATTTACAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                 <  1:4961524/138‑1 (MQ=255)
                                                                                                      aGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                 <  2:5589505/140‑1 (MQ=255)
                                                                                                      aGCCCTTATATTAAACGTGTTTTTATAGGGAAAAACAGACCATCTACTAGCTTTTTTACGCTACACAAGGGTGACATTTAACTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                 <  2:7478786/140‑1 (MQ=255)
                                                                                                          cTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACt                             >  1:1494358/1‑140 (MQ=255)
                                                                                                            tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                           >  1:7469371/1‑140 (MQ=255)
                                                                                                            tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATATTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                           >  2:3374884/1‑140 (MQ=255)
                                                                                                              aaaaaaACCTGATTTTATCGCGAAAAACCGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAAGTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                         <  1:2391595/140‑1 (MQ=255)
                                                                                                              aaaTAAACCTGATTTTATCCCGCAAAACAGACCATAGCCCAGCTTTTTTCCGATACACAAGGGTGACAAGGAACTTGTGGTAAAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                         <  1:1654631/140‑1 (MQ=255)
                                                                                                               aaTAAACCTGATTTTATAGCGCAAAACCGACCATATACGCGCTTTTTTACGCTTCACAAGGGTGACAATGAACGTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTgg                        <  2:680792/140‑1 (MQ=255)
                                                                                                                   accctttttttATAGCGCAAAACCCACATTATCCGAGCTTTTTTACGCTTCACATGGTTCACAATCAACTTGTGGTACATTTTAAAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  1:4423093/133‑1 (MQ=255)
                                                                                                                   acccTGAGTTTCAAGCCCCAAACAGCCCATCTCCGAGCTTTTTTACGGTTCCCAAGGGTGACACGGACCGTGGGGTACAATTTACAACCTACGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:3995983/138‑1 (MQ=255)
                                                                                                                   aacttttttttATAGCGCAACACACACCATATACGCGTTTTTTTACGCTTAACATGGGTGACAATGAATTTTTGGTACAATTTAAAAAGTTGGTAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  1:2358203/133‑1 (MQ=255)
                                                                                                                   aaCCTTTTTTTATAGCGAAAAACACACCATATACGAGATTTTTTCCGATTCACCCGGGTGACAATTAAGTTGTGTTAAATTTTTAAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  1:3684443/140‑1 (MQ=255)
                                                                                                                   aaCCTTTTTTTATAGCCCAAAACCCACCATATACGAGATTTTTTACGCTTCACCTGGGTGACAATGAACTTGTGGTACAATTTTCAACCTTCGGAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  1:794390/140‑1 (MQ=255)
                                                                                                                   aaCCTTATTTTATCCCCCAAAACAGACAATATCCCCGATTTTTTACCCTACCCACGGGTCACATTGAACTTGTCTAAAAATTTTCAAAATACGTAAAAACGCCGGGGTGGGGAATCGAAAAGCCGATTACTTTTGGAACg                    <  1:3616967/140‑1 (MQ=255)
                                                                                                                   aaCCTTATTTAATACCGCAAAACAGACCCTATACGAGCTTTTTTACGCTTCACACGGGTGACACTGACCTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  1:6753340/140‑1 (MQ=255)
                                                                                                                   aaCCTGTTTTTATAGCGCAAACCAGCCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAAGGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:2036657/140‑1 (MQ=255)
                                                                                                                   aaCCTGATTTTATAGCGCAAACCAGACCATATACGAGGTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:4251940/140‑1 (MQ=255)
                                                                                                                   aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGAAAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:1444357/140‑1 (MQ=255)
                                                                                                                   aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:5782713/140‑1 (MQ=255)
                                                                                                                   aaCCTGATTTTATAGCGCAAAAACTACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAGTTGTGGTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:3427223/140‑1 (MQ=255)
                                                                                                                    aCCTTATTTTATAGCCCAAACCACACCATATACCAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTCCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                   <  1:6168210/140‑1 (MQ=255)
                                                                                                                    aCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                   >  1:461517/1‑140 (MQ=255)
                                                                                                                    aCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                   >  1:2038772/1‑140 (MQ=255)
                                                                                                                    aCCTGATTTTATAGCGAAAAACACACCATATACGCGATTTTTTACGCTTCCCACGGGTGACAATGAACTTGTGGAACAATTTACAAACTGCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                   <  1:28489/140‑1 (MQ=255)
                                                                                                                     ccTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                  <  2:7031184/140‑1 (MQ=255)
                                                                                                                     ccTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                  <  2:3271423/140‑1 (MQ=255)
                                                                                                                     ccTGATTTTATAGCGCAAAACACACCATATACGAGCTTTTTTACGGTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                  <  2:1164931/140‑1 (MQ=255)
                                                                                                                      cttaTTTAATCGCGCCAACCACACCATATACCCCCTTTTTTACGCTACACACGGGTGACAATGCACTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAg                 <  1:7625540/137‑1 (MQ=255)
                                                                                                                        gATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGACCTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGa               <  2:6451316/140‑1 (MQ=255)
                                                                                                                         tttttATCCCGCAAAACAGACCATAACCGCTGTTTTTTAGGCTTCACACGGGGCAAATGTAAGTTTTGGAAAAATTTTCAACCTTGCCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt              <  1:1021519/139‑1 (MQ=255)
                                                                                                                         tttttATAGCGCAAAAAACACCATATACGAGCTTTTTTACGCTTAACAAGGGTTACAATGAACTTGTGGTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt              <  2:3014063/139‑1 (MQ=255)
                                                                                                                         tttttATAGCGAAAAACACACCATATACGATTTTTTTTACGATACACAAGGGTTACAATGAACTTGTGTTAAAATTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt              <  1:4538835/139‑1 (MQ=255)
                                                                                                                         aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt              >  2:6671430/1‑140 (MQ=255)
                                                                                                                         aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGATTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCTGAAAAACGACGGGGTGGGGAATCTCAAAGCCGATTACTTTTGGAACGCAGGAt              >  2:3602083/1‑140 (MQ=255)
                                                                                                                           tttATAGCGCAACACAGACCATATCCGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa            <  2:1484417/140‑1 (MQ=255)
                                                                                                                           tttATAGCGCAAACCAGACCATATACGAGCTTTTTTACGCTTCCCAAGGGTGACAATGAACTTGTGGTACATTTTTCAAACGTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa            <  2:37709/140‑1 (MQ=255)
                                                                                                                           tttATAGCGCAAAACCCACCATATACGAGCTTTTTTCCGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa            <  2:2534847/140‑1 (MQ=255)
                                                                                                                           tttATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATTAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa            <  2:7325761/140‑1 (MQ=255)
                                                                                                                           tttATAGCGAAAAACAGACCATATACGAGATTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa            <  2:5857025/140‑1 (MQ=255)
                                                                                                                              ataGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACat         >  2:6187/1‑140 (MQ=255)
                                                                                                                              ataGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACat         >  1:351778/1‑140 (MQ=255)
                                                                                                                                   gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    <  2:2631299/124‑1 (MQ=255)
                                                                                                                                   gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                    >  1:2631299/1‑124 (MQ=255)
                                                                                                                                    cAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGTAACGCAGGATAACATATTGCg   >  1:4082427/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                      
ATCAGATCAAAGCAAGACAATCAGGTTTTCGGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGA  >  NZ_CP034943/3561846‑3562118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: