Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,562,015 A→C 12.1% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,562,0150AC12.1% 788.9 / 12.8 383intergenic (‑661/‑137)EO946_RS18300/EO946_RS21105response regulator/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (181/124);  new base C (2/67);  total (183/200)
Fisher's exact test for biased strand distribution p-value = 2.42e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATCA  >  NZ_CP034943/3561879‑3562152
                                                                                                                                        |                                                                                                                                          
aTCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                         <  2:1936070/140‑1 (MQ=255)
aTCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                         <  2:3566644/140‑1 (MQ=255)
      aCGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACaa                                                                                                                                   <  1:6888754/140‑1 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGGCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTGACGCTTCACAAGGGTGACAATGAACTTGTGGTAGaa                                                                                                                  >  1:5723440/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCCATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  1:797621/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGAGAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTTCGCTTCAGAAGGGTGACAATGAAGTTGTGGTACaa                                                                                                                  >  2:633943/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  1:3809148/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  2:2171524/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  2:6033870/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  2:7196045/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAACGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  2:5896449/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                                                                 >  2:1698425/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                       >  2:4606321/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                       >  1:2247433/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                       >  1:918954/1‑140 (MQ=255)
                                           aTGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACg                                                                                             >  2:2603110/1‑140 (MQ=255)
                                           aTGGCCAATTAATAGGAAAACGCGCAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAAGGAACTTGTGGTACAATTTTCAAACTTCGGAACAACg                                                                                             <  2:6657330/140‑1 (MQ=255)
                                             ggCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGcc                                                                                           >  2:3687499/1‑140 (MQ=255)
                                              gCCACTTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCg                                                                                          >  2:7647264/1‑140 (MQ=255)
                                              gCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACCAGCTTTTTTACGCTTCACAAGGGTGACAATCAACTTGTGGGAGAATTATCAAACATCGGAAAAACGCCg                                                                                          >  2:5083246/1‑140 (MQ=255)
                                                cAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTtg                                                                                                                          >  1:5467423/1‑106 (MQ=255)
                                                      aTAGCACAAAGCGGAAGCCCTTAAAATAAACCTTATTTTATCGCCCAAAACACACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGTAAAAACGCCGGGGTgggg                                                                                  <  1:6582994/140‑1 (MQ=255)
                                                             aacgCGGACGCCCTTAAAATAACCCTGATTTTATAGCGCAAACCCCCCCATATACGCGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           <  2:6275454/137‑1 (MQ=255)
                                                             aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGACTCGCa                                                                           >  1:4150473/1‑140 (MQ=255)
                                                             aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           <  2:1481025/140‑1 (MQ=255)
                                                             aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           >  2:4826500/1‑140 (MQ=255)
                                                             aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGGGGGGAATCGCa                                                                           >  2:547980/1‑140 (MQ=255)
                                                             aaaGCGGAAGCCATTAAAAAAAACTTTTTTTTATAGCGCAAAACACACCATATCCGCGATTTTTTACGCTTCACCCGGGTCACAATCAACTTGTGGTACAATTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCa                                                                           <  1:7436450/140‑1 (MQ=255)
                                                              aaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaa                                                                          >  1:3498632/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATTAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTCCAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  1:470311/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGACTCGCaaa                                                                         >  1:3120457/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  1:7656042/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  2:7124245/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  1:4795600/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTGTTTTACGCATCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  1:705046/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATAGACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGATAGACGCCGGGGTGGGGAAGCGCaag                                                                         >  1:3995983/1‑139 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                         >  1:4873207/1‑72 (MQ=255)
                                                                     accccTTCAAATAAACATTATTTTATAGCGAAACACACACCATATACGACATTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  2:3991620/138‑1 (MQ=255)
                                                                     accccTTAAAATAAACCTGATTTTATAGCGAAAACCAGCCAATAAACGAGGTTTTTAACGGTTCCAACGGGTGACAATCAACGTGTGGTACAATTTACAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  1:4961524/138‑1 (MQ=255)
                                                                     aGCCCTTCAAATAAACCTGATTTTATCGCGCCACACCGCCCATATACGAGCTTTTTTACCCGTCACAAGGGTGACCAGGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  2:4275433/140‑1 (MQ=255)
                                                                     aGCCCTTAAAATAAACCTGATTTTATCGCGCAAAACAGACCATATACGCGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  2:2839980/140‑1 (MQ=255)
                                                                         cTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACt                                                               >  1:1494358/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                             >  1:5782713/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                             >  1:6056361/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                             >  2:4149881/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATATTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                             >  2:3374884/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCGTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGACTCGCAAAGCGGATTACgtt                                                             >  2:6848355/1‑140 (MQ=255)
                                                                             aaaaaaACCTGATTTTATCGCGAAAAACCGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAAGTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                                                           <  1:2391595/140‑1 (MQ=255)
                                                                                tAACCCTTATTTTATAGCGCAAAACACCCCATATACCCGCTTTTTTACGATTCACAAGGGTGAAAATCAACTGGTGGTACAATTTACAAACTACGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  1:7150387/140‑1 (MQ=255)
                                                                                tAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  2:1341832/140‑1 (MQ=255)
                                                                                aaaaCCTGATTTTATAGCGAAAAACAGACAATATCCGCGTTTTTTTCCGATACACAAGGGTGACAATGAACTTGTGGTAAAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  1:621194/139‑1 (MQ=255)
                                                                                 aaaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAAc                                                       >  1:2533216/1‑140 (MQ=255)
                                                                                  aaCCTTTTTTTATAGCGAAAAACACACCATATACGAGATTTTTTCCGATTCACCCGGGTGACAATTAAGTTGTGTTAAATTTTTAAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:3684443/140‑1 (MQ=255)
                                                                                  aaCCTTTTTTTAAAGCGAAAAACCCACCATATCCGCCCTTTTTACCTGTTCCCAAGGGTCACATTGAATTTGTGTTACATTTTGCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:1698425/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATAGCGCAAAACTGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGAAAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:5578692/140‑1 (MQ=255)
                                                                                  aaCCTTATTTAATACCGCAAAACAGACCCTATACGAGCTTTTTTACGCTTCACACGGGTGACACTGACCTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:6753340/140‑1 (MQ=255)
                                                                                  aaCCTGTTTTTATAGCGCAAACCAGCCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAAGGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:2036657/140‑1 (MQ=255)
                                                                                  aaCCTGTTTTTATACCGCAAAACACACGAAATACGTGCTTTTTTACGCTTCACACGGTTGAAACTGACCTTGTGGTAAAATTTTCAAAGTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:6678457/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATCGCCCAAAACACCCCATATACGAGATTTTTTACGCTTCAAAAGGGTCACATTGAAGTTGTGGTACAATTTTCAAACTTCGGAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:3615320/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGAAAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:1444357/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGGGACAATGACCTTGTGGTACATTTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:2366350/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGAAAAACACCCCATATACCAGCTTTTTTACGCTTCACAAGGGTGACAATTAACTTGTGGTAAAATTTACAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:6337324/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGAAAAACACACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:3468458/140‑1 (MQ=255)
                                                                                  aaCCTGATATAATAGCGCAAAACACACCATAACCGAGATTTTTTACGCTTCACAAGGGTCACAATGAACTTGTGGTACAATTTAAAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:5514730/140‑1 (MQ=255)
                                                                                   aCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                                                     >  1:461517/1‑140 (MQ=255)
                                                                                   aCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                                                     >  1:2038772/1‑140 (MQ=255)
                                                                                   aCCTGATTTTATAGCGAAAAACACACCATATACGCGATTTTTTACGCTTCCCACGGGTGACAATGAACTTGTGGAACAATTTACAAACTGCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                                                     <  1:28489/140‑1 (MQ=255)
                                                                                    cctttttttATAGCGAAAAACAGACAATCTACGAGATTTTTTACGCTTCACCAGGGTTACAATGAACTTGTGGTACATTTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                    <  1:7257253/135‑1 (MQ=255)
                                                                                    ccTTATTTTATAGCCCAAAACACACCATATCCGAGATTTTTTACGCTACACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                    <  1:5200840/140‑1 (MQ=255)
                                                                                    ccTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                    <  2:3271423/140‑1 (MQ=255)
                                                                                    ccTGATTTTATAGCGCAAAACACACCATATACGAGCTTTTTTACGGTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                    <  2:1164931/140‑1 (MQ=255)
                                                                                     cttaTTTAATCGCGCCAACCACACCATATACCCCCTTTTTTACGCTACACACGGGTGACAATGCACTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAg                                                   <  1:7625540/137‑1 (MQ=255)
                                                                                      tGATTTTATAGCGCAAACCACACCATATCCGCGGTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                  <  2:5819486/140‑1 (MQ=255)
                                                                                      tGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                  <  2:5646595/140‑1 (MQ=255)
                                                                                        tttttATCCCGCAAAACAGACCATAACCGCTGTTTTTTAGGCTTCACACGGGGCAAATGTAAGTTTTGGAAAAATTTTCAACCTTGCCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  1:1021519/139‑1 (MQ=255)
                                                                                        tttttATAGCGAAAAACACACCATATACGATTTTTTTTACGATACACAAGGGTTACAATGAACTTGTGTTAAAATTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  1:4538835/139‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  2:6173992/140‑1 (MQ=255)
                                                                                        aTTTTATAGCGAAAAAAACACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTACGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  2:3777894/140‑1 (MQ=255)
                                                                                        aTTTTATAGCCCAAAACACACAATATCCGAGATTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  1:3361984/140‑1 (MQ=255)
                                                                                          tttATAGCGCAAAACCCACCATATACGAGCTTTTTTCCGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                              <  2:2534847/140‑1 (MQ=255)
                                                                                          tttATAGCGCAAAACAGCCCATATACGAGTTTTTTTACGCTACACACGGGTCACAATGACGTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                              <  1:3428931/140‑1 (MQ=255)
                                                                                              taGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACata                                          >  1:1332590/1‑140 (MQ=255)
                                                                                                  gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAATCGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGc                                      >  1:4342243/1‑140 (MQ=255)
                                                                                                   cAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACACTTTTCACACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCg                                     >  1:7564469/1‑140 (MQ=255)
                                                                                                         accccctataCGAGCTTTTTTACGCTTCACACGGGTGACACTGAAATTGTGGTACAATTTTCAAACTTCGGAAATACGCCGGGGTGGGGAATCGCAAAGACGATTACTTTTGGAACGCAGGATAACATATTGCgagagat                               <  2:7380613/134‑2 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                             >  2:5342238/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                             >  2:3896523/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                             >  2:2963776/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGATTCACAAGGGTGACAATGAACTTGTGGTACAATTATCAAACTTAGGAAAAAAGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGATAGAACa                             >  2:3076589/1‑140 (MQ=255)
                                                                                                                  aCGAGCTTTTTGACGCTTCACCAGGGTGACAATGAACGTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTc                      <  2:6453844/140‑1 (MQ=255)
                                                                                                                    gAGCTTTTTTACGCTTCACATGGGTGACAATGAAGTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCt                    <  1:4494056/140‑1 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                              <  2:4428899/111‑1 (MQ=255)
                                                                                                                         tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt               >  2:866779/1‑140 (MQ=255)
                                                                                                                          ttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTa              >  2:7075112/1‑140 (MQ=255)
                                                                                                                           tttcccattCACAAGGGTGACAAGTAACTTGTGGTACAATTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa             <  1:5555025/133‑1 (MQ=255)
                                                                                                                           tttCCGCTTCACAAGGGTGACAATGAACTTTTGGTACAATTTTCAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa             <  2:4941375/140‑1 (MQ=255)
                                                                                                                           tttACGCTTCACACGGGTGACAATGAACTGGTGGGACAATTGTCAAACGTCGGAACAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa             <  2:4728354/140‑1 (MQ=255)
                                                                                                                           tttACGCTTCACACGGGTGACAATGAAATTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa             <  1:535220/140‑1 (MQ=255)
                                                                                                                           ttgaCGCTTCCAAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa             <  2:6736733/137‑1 (MQ=255)
                                                                                                                                    cacaCGGGTGACAATTAACTTGTGGTACAATTTTCAAACTGCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt    <  1:1202038/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATCA  >  NZ_CP034943/3561879‑3562152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: