Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,256 G→C 5.4% A26P (GCA→CCA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2560GC 5.4% 1738.1 / 5.8 657A26P (GCA→CCA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base G (405/191);  new base C (2/52);  total (412/245)
Fisher's exact test for biased strand distribution p-value = 9.15e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 696 total aligned reads displayed.

CTGTTTGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTA  >  NZ_CP034943/316117‑316394
                                                                                                                                            |                                                                                                                                          
cTGTTTGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACaaagaaag                                                                                                                                            <  1:139364/140‑1 (MQ=255)
  gtgtgtgtgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCa                                                                                                                                          >  1:7506508/4‑140 (MQ=255)
  gTTTGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCa                                                                                                                                          >  1:1669356/1‑140 (MQ=255)
  gTTTGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCa                                                                                                                                          >  2:4017068/1‑140 (MQ=255)
  gTTTGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATATAAAATTTTTGGGAAACAAACAAAGAAAGCa                                                                                                                                          >  1:5138613/1‑140 (MQ=255)
   tttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAg                                                                                                                                         >  2:1291812/1‑140 (MQ=255)
   tttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAg                                                                                                                                         >  2:1013707/1‑140 (MQ=255)
   tttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAg                                                                                                                                         >  2:4319187/1‑140 (MQ=255)
    ttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACACACAAAGAAAGCAGt                                                                                                                                        >  2:496350/1‑140 (MQ=255)
    ttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGt                                                                                                                                        >  1:3463624/1‑140 (MQ=255)
    ttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGt                                                                                                                                        >  1:6447646/1‑140 (MQ=255)
    ttGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGt                                                                                                                                        >  1:2088032/1‑140 (MQ=255)
      gtgtgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                      <  1:764998/140‑1 (MQ=255)
             aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGCt                                                                                                                               >  1:5622683/1‑140 (MQ=255)
                ttACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTCAAATTTTTGGGACACAAACAAAGAAAGCAGTTCAAATGCTTGc                                                                                                                            >  2:415767/1‑140 (MQ=255)
                        gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGGTTGCCAACGGaa                                                                                                                    >  2:6357134/1‑140 (MQ=255)
                        gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                    >  1:3258504/1‑140 (MQ=255)
                        gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                    >  2:1958155/1‑140 (MQ=255)
                        gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                    >  2:188863/1‑140 (MQ=255)
                           ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACCAAGAAAGCAGTTCAAATGCTTGCCCACGGAAAGa                                                                                                                 >  2:6564034/1‑140 (MQ=255)
                           ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATGACGATAAAAGTCGAGAAAGTATCTATAATTTTTGGGAAACAATCAAAGACAGCAGGTCAAATGCTTGCCAACGGACAGa                                                                                                                 >  2:2686855/1‑140 (MQ=255)
                           gtACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                 >  2:2537377/2‑140 (MQ=255)
                             aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                               >  1:3757834/1‑140 (MQ=255)
                             aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTAGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAATTGCTTGCCAACGGAAAGACa                                                                                                               >  1:92156/1‑140 (MQ=255)
                                   ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAATCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                         >  2:5285611/1‑140 (MQ=255)
                                   ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                         >  2:23152/1‑140 (MQ=255)
                                     gagacaggTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                       <  2:6002523/133‑1 (MQ=255)
                                     aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAACCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                       <  2:6666758/140‑1 (MQ=255)
                                     aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                       <  1:132156/140‑1 (MQ=255)
                                           tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                 >  1:109838/1‑140 (MQ=255)
                                           tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                 >  1:3570659/1‑140 (MQ=255)
                                             tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                      >  2:4380150/1‑101 (MQ=255)
                                              ttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGaa                                                                                              >  1:6919204/1‑140 (MQ=255)
                                                    tgttgtaaaaaTATGATTGTATCTCACAAACAAATTAAGATAAAAGTCGAGAACTTAAATAAATTTTTTGGCAAAAAAAAAAATAAACCAGTTAAAATGGTTCCCACCGCAAAAAAAAAAAAAGATATCCTGAAAGCGAc                                                                                        <  1:7184965/133‑1 (MQ=255)
                                                    gggAGGGAAAATATGAGTGTATATCAGAAACAAATTAATATAAAAGTCGAGAAAGTATCTAAAATTTTTGGTAAAAAAACAAATAAAGCATTTAAAATGCTTGCCAACGCAAAGAAAAAAAAAGAGATCCTGAAAGCGAc                                                                                        <  2:855905/139‑1 (MQ=255)
                                                      gAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCg                                                                                      >  1:3106044/1‑140 (MQ=255)
                                                      gAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCg                                                                                      >  1:4022486/1‑140 (MQ=255)
                                                       aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                     >  1:1231329/1‑140 (MQ=255)
                                                       aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                     >  1:2378429/1‑140 (MQ=255)
                                                       aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                     >  1:2923096/1‑140 (MQ=255)
                                                       aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                     >  2:7275020/1‑140 (MQ=255)
                                                          gAAATTATTATTGTAGATGATAAACATATTATTATAAAAGTCTAGAAAGTTTATAAAATTTTTGGTACAAAAACAACTAATCCAGTACATTTGGTTGCCACCGCAAAGACAAAAAAAGAGATCGTTAAAGCGACCGGATc                                                                                  <  2:7164397/140‑1 (MQ=255)
                                                            aaaTATGAGTGTAGAGGAGAAACCAATTAAGATACAAGTCGAGAAAGTATCTAAAATTTTTGGGCAACAAACAAAGAAAGCAGTTCAATTGCTTGCCAACGGAAAGAGACTAAAAGAGATCCTGAAAGCGAGCGGCTCag                                                                                >  1:1000992/1‑139 (MQ=255)
                                                                atGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGt                                                                            >  2:1536419/1‑140 (MQ=255)
                                                                    gtttacaTGACAAAAAAATAAACATAAAAGTCGATAAAGTATCTAAAATTTTTGGTAAAAAAAAAAAGAACGCTTTTCAATGGCTTGCCAACGCAAAGAAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTggg                                                                        <  1:6631063/134‑1 (MQ=255)
                                                                     tgtAGATGAGAAACCAATTAAGATCAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                       >  2:5874448/1‑140 (MQ=255)
                                                                     tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                       >  1:6134398/1‑140 (MQ=255)
                                                                       tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                     >  2:2028089/1‑140 (MQ=255)
                                                                             agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGAGACCGGATCAAACGTTGGGGTAAATCaa                                                               >  2:3205033/1‑140 (MQ=255)
                                                                              gaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAg                                                              >  1:432338/1‑140 (MQ=255)
                                                                               aaaCCACTTAACAAAAAACTCCATAACCTATCTAAAATTTTTGGCAAACAAACAAACAAAGCAGTTCAAATGCTTCCCACCGCAAACACAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                             <  2:4875090/140‑1 (MQ=255)
                                                                               aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                             >  1:2784752/1‑140 (MQ=255)
                                                                               aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                             >  1:1951947/1‑140 (MQ=255)
                                                                               aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                             >  1:2213532/1‑140 (MQ=255)
                                                                               aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGc                                                             >  2:1606473/1‑140 (MQ=255)
                                                                               aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAACAAAGCAGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                             <  2:7364279/140‑1 (MQ=255)
                                                                                 aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                           >  1:7040904/1‑140 (MQ=255)
                                                                                  acAATTAACATAAAAGACGAGAACGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGa                                                          <  2:5185759/139‑1 (MQ=255)
                                                                                    aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGAtt                                                        >  2:4597423/1‑140 (MQ=255)
                                                                                       tAATATAAACGTCGAGAAAGTATCTAAAATTTTGGGCAAACAAACAAAGAACGCCGTAAAAATGCTTGCCACCGTAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                     <  2:1118560/140‑1 (MQ=255)
                                                                                       tAAGATAAAAGTCGATAAATTATCTAAAATTTTTGGGAAACAAAAAAAGAAAGCAGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                     <  2:3463624/140‑1 (MQ=255)
                                                                                       tAAGATAAAAGTCGACAATGTATCTAAAATTTTTGGGAAACAAAAAAACAAAGCAGTTCAAAGGCTTGCCAACGGAAACACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                     <  2:6913464/140‑1 (MQ=255)
                                                                                       tAACATAAAAGCCGACAAAGTATCTAAAATTTTTGGCAAACAACCAAAGAACCCAGTTCAAATCCGTGCCAACGCAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                     <  2:1359647/140‑1 (MQ=255)
                                                                                       aaacaaaaaaGTCGACAAATTAAAAAAATTTTTGGCCAAAAAAACAAACACAGCATTACAAATGCTTGCCAACGCAAACACAAAAAAATACACCCTCACCGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                     <  1:654878/134‑1 (MQ=255)
                                                                                       aaaGATAACCGTCCAGACAGTCTCTAAATTTTTGGGCAAACAAAAAAACAAAGCCTTTAAAAGGCGTGCCACCCCAAACAAAAAAAAACAGACCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                     <  1:4596162/139‑1 (MQ=255)
                                                                                         aGATACAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                   <  2:1762385/140‑1 (MQ=255)
                                                                                         aGATAAACGTCGATAAAGTATCTAAAATTTTTGGCAAAAAAACAAAGAAAGCATTAAAAATGCTTGCAAACGCAAACAAAAAAAAAGAGAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                   <  1:5285611/140‑1 (MQ=255)
                                                                                         aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                   <  2:5644248/140‑1 (MQ=255)
                                                                                         aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGCACAAAAACAAACAACGCAGTACAAAGGCTTGCAAACGCAAACACAAAAAAACATATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                   <  2:1199708/140‑1 (MQ=255)
                                                                                           aTAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCACGCAGATTTTGATGt                                                 >  2:6813283/1‑140 (MQ=255)
                                                                                             aaaaGTCGAGAAAGTATCTAAAATTTTTGGGACACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                               >  2:703625/1‑140 (MQ=255)
                                                                                             aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                               >  2:6452083/1‑140 (MQ=255)
                                                                                                aGTCGAGAAAGTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCCTTTCAATTGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                            <  2:2871612/140‑1 (MQ=255)
                                                                                                      taatttatataATATTTTTCTAAAAAAAACAACAAAACCAGTAAATTTGGTTGCAACCGCAAACACAAAAAAATTCATCGTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                      <  1:2645606/135‑1 (MQ=255)
                                                                                                      taaaGTATCTAAAATTTTGGGTAAAAAAACAAACAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAATACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                      <  2:369826/139‑1 (MQ=255)
                                                                                                      caaaGTATCTAAAATTTTGGGCAAACAAAAAAATAAAGCATTTAAAATGCTTGCCAACGTAAAGAAAAAAAAAGACATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                      <  1:5529921/139‑1 (MQ=255)
                                                                                                      caaaGCATCTAAAATTTTTGGGAAACAAACAACCAACGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                      <  2:3611950/139‑1 (MQ=255)
                                                                                                            aTCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                >  1:4996900/1‑140 (MQ=255)
                                                                                                              cTACAATTTTGGGCACACAAACAAAGAAAGCAATTCAAATGCTTGCAACCGCTACGACAAAAACAGAGAGCCGGAAAGCGACCGGCTCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                              <  2:7436413/140‑1 (MQ=255)
                                                                                                                aaaaTTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                            >  1:597383/1‑140 (MQ=255)
                                                                                                                    tttttGGCAAAAAAAAAAAGAACGCATTTCAAATGATTGCAAACGAAAAGAAAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                        <  2:5944174/140‑1 (MQ=255)
                                                                                                                      tttGGGAAACAAACAAAGAAAGCAGTTCAAAGGCTTGCAAACGCAAACACAAAAAAAGATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                      <  1:6245416/140‑1 (MQ=255)
                                                                                                                      tttGGCAAACAAACAAAGAACGCAGTTAAAATCCTTGCCAACGCAACCCCAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                      <  2:6591162/140‑1 (MQ=255)
                                                                                                                      tttGGCAAACAAAAAAAGAAAGCAGTTCAAATGATTGCCAACTGAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                      <  1:1796801/140‑1 (MQ=255)
                                                                                                                      tttGGCAAAAAAACACACAAACCCGTACACAGGCTTGCCCACGCAAACACAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                      <  1:6346945/140‑1 (MQ=255)
                                                                                                                      ttctgccaaacaaacaaacaaacCAGTAAAAAGGCTTCCCAACGAACACACAAAAAAACACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                       <  1:4849329/133‑1 (MQ=255)
                                                                                                                      gttGGCAAACAACCAAACAACGCCGTCAAAAGGCTGGCCAACGTAAACACAAAAAAAGACATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                      <  1:7410273/139‑1 (MQ=255)
                                                                                                                       ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCACGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                     >  2:3394453/1‑140 (MQ=255)
                                                                                                                       ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                     >  1:6751020/1‑140 (MQ=255)
                                                                                                                        tGGCAAACAAACAAAGAAACCATTTCAAATGATTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                    <  2:4753508/140‑1 (MQ=255)
                                                                                                                        ggggAACCAACAAAAGAAAGCATTTCAACTCCGTGCCACCTGAACCACAAAAACTCTGACCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCACGCAGATTTTGAGGTATATGATGGTGAGATATTTGTCATCATgg                    <  1:2519599/139‑1 (MQ=255)
                                                                                                                           cgAAACAAACAAATAAAGCAGTTCATCTGCTTCCCAAGGGACATACAAAAAAAGCCAGCCTGAAAGCGACCGGATCTACCGTTTGGGTAAATCAAGCAGATTTTGATTTATATGATGGTGAGATATTTGTCATCATGGGt                  <  1:4139041/139‑1 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCACCGGAAAGACAAAATAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAGTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa  >  2:3036850/1‑140 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTCTATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa  >  1:3385863/1‑140 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGtc  >  2:7587230/1‑139 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa  >  2:6285564/1‑140 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa  >  2:61682/1‑140 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa  >  1:5789220/1‑140 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTAATCATGGGTCTATCAGGGAGCGGTa  >  2:2127493/1‑140 (MQ=255)
                                                                                                                                           agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATAAGGGAGCGGTa  >  2:5421491/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CTGTTTGTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTA  >  NZ_CP034943/316117‑316394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: