Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,569,289 T→G 7.4% E116D (GAA→GAC mbl ← cell shape‑determining protein Mbl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,569,2890TG 7.4% 1489.4 / 12.0 580E116D (GAA→GACmblcell shape‑determining protein Mbl
Reads supporting (aligned to +/- strand):  ref base T (234/269);  new base G (74/2);  total (309/271)
Fisher's exact test for biased strand distribution p-value = 6.23e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 600 total aligned reads displayed.

CGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTGTTACTTCAAAGTCAGCA  >  NZ_CP034943/3569151‑3569427
                                                                                                                                          |                                                                                                                                          
cgcgATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCACCTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGctt                                                                                                                                           >  2:4543675/1‑140 (MQ=255)
cgcgATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGGGGctt                                                                                                                                           >  1:113209/1‑140 (MQ=255)
cgcgATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGctt                                                                                                                                           >  1:1295906/1‑140 (MQ=255)
cgcgATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGctt                                                                                                                                           >  1:5985912/1‑140 (MQ=255)
cgcgATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGctt                                                                                                                                           >  1:5694730/1‑140 (MQ=255)
 gcgATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAAGTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGgttc                                                                                                                                          >  1:211668/1‑140 (MQ=255)
   gATATCCGTCGTCCCGCCTCCGTTGTCTACAACCATGTTACCGCTTGCCTTGAATATTTCCATACCCGAGCCCTTAGCGGAAACTTTAGGTTCTAATTCAAGTTAGACAAGATAAACGCCGCTGTGTTCTGCGGcatcat                                                                                                                                        >  2:1766944/1‑138 (MQ=255)
      atCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGa                                                                                                                                     <  2:1372892/140‑1 (MQ=255)
      atCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGa                                                                                                                                     <  2:596887/140‑1 (MQ=255)
        ccGTCGTCCCGCCTCCGATGTCTACAACCCTGTTACCGCTTGGCTGGAATTTTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGAtt                                                                                                                                   <  1:2878772/140‑1 (MQ=255)
        ccGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGAtt                                                                                                                                   <  2:3756975/140‑1 (MQ=255)
             gtcCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTGTGATTGCttt                                                                                                                              >  2:218631/1‑140 (MQ=255)
                   ccTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTc                                                                                                                        <  1:1780400/140‑1 (MQ=255)
                     tCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCaa                                                                                                                      <  1:3229034/140‑1 (MQ=255)
                      ccGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAAc                                                                                                                     <  1:897303/140‑1 (MQ=255)
                      ccGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAAc                                                                                                                     <  1:3616073/140‑1 (MQ=255)
                        gATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACgg                                                                                                                   <  2:3140618/140‑1 (MQ=255)
                          tGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGAt                                                                                                                 <  1:4717683/140‑1 (MQ=255)
                              tACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTCGTTGTTTGCTTTTTTCTCAACGGAAGTac                                                                                                             >  1:5265654/1‑139 (MQ=255)
                                cAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAata                                                                                                           >  2:7248286/1‑140 (MQ=255)
                                   ccATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGGGTGTTTGATTGCGTTTTGCTCAACGGATGTAAttttg                                                                                                        >  1:4260191/1‑139 (MQ=255)
                                   ccATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTCGTTGATTGCGTTTTGCTCAACGGATTTAATATtc                                                                                                        >  1:748342/1‑140 (MQ=255)
                                     aTGGTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgt                                                                                                      <  2:6867454/140‑1 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAAGGTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTGCTTTGTTTGGTTTTTGCTCCACGGATGTAATATtcgtc                                                                                                     >  1:5501638/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGGTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgtc                                                                                                     >  2:2692872/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGGGTCTTTGATTTGTTTTTGCTCAACGGATTTAATATtcgtc                                                                                                     >  1:487024/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTGTTTGATTGCTTTTTTCTCAACGGATGTAATATtagtc                                                                                                     >  1:6610908/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGCTTGCTTTTTGCTCAACGGATGTAATATtcgtc                                                                                                     >  2:542284/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATg                                                                                                                >  2:6618478/1‑129 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtggtc                                                                                                     >  2:234790/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgtc                                                                                                     >  2:4110671/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgtc                                                                                                     >  2:152573/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgtc                                                                                                     >  2:6806589/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtagtc                                                                                                     >  2:5677106/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgtc                                                                                                     >  2:2408988/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGGGTTTGATTGGTTTTTGCTCAACGGATGTAATATtggtc                                                                                                     >  1:3841378/1‑140 (MQ=255)
                                      tGTTACCGCTTGGCTGGAATATTTACATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGGTTGTTTGATTTCTTTTTTCTCAACGGATGTAATATtcgtc                                                                                                     >  1:5375353/1‑140 (MQ=255)
                                        ttACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCgg                                                                                                   >  2:3993471/1‑140 (MQ=255)
                                        ttACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCgg                                                                                                   >  2:7343169/1‑140 (MQ=255)
                                        ttACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCGTTGATTGCTGTTTGCTCAACGGATGTAATATtcttagg                                                                                                   >  1:7616671/1‑135 (MQ=255)
                                        ttACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTGTTTGATTGGTTTTTGCTCAACGGATGTAATATTGGTCgg                                                                                                   >  1:7208804/1‑140 (MQ=255)
                                        ttACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTGTTTGATTGGTTTTTGCTCAACGGATGTAATATTCGTCgg                                                                                                   >  1:7319914/1‑140 (MQ=255)
                                        ttACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTGTTCTGCGGGTTGGTTGGTTGCTTTTTGCTCAACGGATGTGatatgggtggg                                                                                                   >  1:4623317/1‑133 (MQ=255)
                                         tACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCggg                                                                                                  <  2:4692271/140‑1 (MQ=255)
                                         tACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCggg                                                                                                  <  1:5019025/140‑1 (MQ=255)
                                         tACCGCTTGGCTGGAATATGTCCATACCCGCGCCGATAGCGGCAACTGTAGGTTCTTCTTCCAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCggg                                                                                                  <  2:5105045/140‑1 (MQ=255)
                                          aCCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGgc                                                                                                 <  1:152573/140‑1 (MQ=255)
                                              cTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTGTGCGGCTTGTTTTATTGGTTTTTGGTGAACGGATGTAAAATTGTTCGGgccgca                                                                                             >  1:3096419/1‑140 (MQ=255)
                                              cTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGCTGTAATATTCGTCGGgcagca                                                                                             >  2:2091132/1‑140 (MQ=255)
                                                tGGCTGGAATATTTTCATACCCGCGCCGATAGCGGCAAGTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCaaa                                                                                           >  2:6225698/1‑140 (MQ=255)
                                                 ggCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGgca                                                                                                <  2:5152147/134‑1 (MQ=255)
                                                 ggCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTGTTTGCTCAACGGATGTAATATTCGTCGGGCAGCaact                                                                                          >  2:6777897/1‑138 (MQ=255)
                                                          atTTCCCGACCCGCGCCGATAGCGGCACCTTTAGGTTCTTCTTCCAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATgcg                                                                                 <  1:2550582/140‑1 (MQ=255)
                                                          atTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATgcg                                                                                 <  1:6098052/140‑1 (MQ=255)
                                                          atTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATgcg                                                                                 <  1:5762157/140‑1 (MQ=255)
                                                          atTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATgcg                                                                                 <  1:342829/140‑1 (MQ=255)
                                                          agttCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATAGTCGTCGGGCAGCAAATGAGCATgcg                                                                                 <  1:6277660/138‑1 (MQ=255)
                                                             tCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCgg                                                                              >  2:6476347/1‑140 (MQ=255)
                                                             tCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCgg                                                                              <  1:7150280/140‑1 (MQ=255)
                                                                 tACCCGCGCCGAGAGCGGCAACTTTAGGTTCTTCTTCAAGGGACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGtttt                                                                          <  1:1032486/140‑1 (MQ=255)
                                                                     cgcgCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTCGTTGATTGCTTTTTGCTCAACGGATGTAATATTGGTGGGGGAGCAAATGAGCCTGCGCGGTTTTgtgt                                                                      >  1:6363268/1‑137 (MQ=255)
                                                                     cgcgCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCGGCGGCGTCTTTGATTGCGTTTTGGGCAACGGATGTACTATTCGTCGGGCAGCAAATGAGCCTGCGCGGTTTTgtga                                                                      >  1:5605123/1‑140 (MQ=255)
                                                                       cgcCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTGGTTGATTGGGTTTTGCTGAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGGTTTGAGaaa                                                                    >  1:741318/1‑139 (MQ=255)
                                                                       cgcCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATAGTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAAc                                                                    <  2:3545562/140‑1 (MQ=255)
                                                                         ccGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACaa                                                                  <  2:6976743/140‑1 (MQ=255)
                                                                         ccGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACaa                                                                  <  2:3297697/140‑1 (MQ=255)
                                                                           gATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGc                                                                <  2:7184225/140‑1 (MQ=255)
                                                                              aGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCGTCTTTGATTGCTTTTTGCTAAAAGGATGTTAAATTCGTCGGGCAGCAAATTATCATGCGCGGTTTTTAGAACAAACCtt                                                             >  1:1438949/1‑140 (MQ=255)
                                                                                  gCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTATTCGGCGTCTTTTATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCCGCAAAATAGCATGCGCGGTTTTGAGAAAAAACCttttta                                                         >  1:699119/1‑138 (MQ=255)
                                                                                  gCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCACTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATtcgtcg                                                                                                    <  2:2514677/97‑1 (MQ=255)
                                                                                       tttAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTGTGGGGCGTCTTTGATTGCTTTTTTGTCAACGGATGTTATATTGGTCGGGCAGCAAAATAGCATGCGCGGTTTTGTGAACAACCCTTTTAAAttaa                                                    >  1:4428613/1‑138 (MQ=255)
                                                                                         tAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGc                                                  <  2:812492/140‑1 (MQ=255)
                                                                                             ttcttcttcAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGt                                              <  1:4520186/140‑1 (MQ=255)
                                                                                                ttcttcAAGGTACACATGTTTACCGCCGCTTTTTTGTGCGGCTTCTTTGATTGCTTTTTGCTCAAAGGATGTAATATTCGTCGGGCAGCAAATGAGCCTGCGCGGGTTTGAGAACAAGCCTTTTACATTGAGCTTGTtaa                                           >  1:6155573/1‑140 (MQ=255)
                                                                                                 tcttcAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTGTTTGATTGGTTTTTTCTCAACGGATGTAAAAATCGGCGGGCCGCCAAAGAGCAAGCGCCGGTTTGAGAACAAACCGTTTACATTCAGCGTGTtaat                                          >  1:6714029/1‑140 (MQ=255)
                                                                                                   ttcAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTtaataa                                        <  2:487024/140‑1 (MQ=255)
                                                                                                     cAAGGTACACATGTGTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGACGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATaaaa                                      <  1:6829321/140‑1 (MQ=255)
                                                                                                         gTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCt                                  <  2:3042259/140‑1 (MQ=255)
                                                                                                         gTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCt                                  <  2:1196754/140‑1 (MQ=255)
                                                                                                          tACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCtt                                 <  1:4025429/140‑1 (MQ=255)
                                                                                                            cacaTGTTTACCGCCGCTTTTTTCTTCGGCGTAGTTGATTGCTTTTTGCTCAACGGATTTAATATTCGTCGGGCAGCCAAAGAGCAAGCGCGGTTTTGAGAACAACCCATTTACATTAATCTTGTTAATAAAAttcataa                               >  1:1901090/1‑134 (MQ=255)
                                                                                                            cacaTGTTTACCGCCGCTTTTTTCTGCGGCTTGTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTGGGGCAGCAAATGAGCAATCGCGGGTTTGAGAAAAAGCCGTTTAAATTAAGCTTGTTAATAAAAATCTTCa                               >  1:4141271/1‑140 (MQ=255)
                                                                                                            cacaTGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGc                                                                >  2:1090747/1‑107 (MQ=255)
                                                                                                            cacaTGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCa                               >  2:7628537/1‑140 (MQ=255)
                                                                                                               aTGTGTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCa                            <  2:7516965/140‑1 (MQ=255)
                                                                                                                  tttACCGCCGCTTTTTTCTGCGGGTTCTTTGATTGCTTTTTGCTCAACGGCTGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACAATAAGCTTTTTAATAAAATGCTTCAGCATTg                         >  1:5825103/1‑140 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGCTTGTTTGATTTGTTTTTGCTCAACGGCTGTAATATTCGTCGGGGAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCATTTACAATAAGCTTGTTAATAAAATGCTTAAGCagttgt                       >  1:2778284/1‑135 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGTGAACAAACCTTTTTCATTCAGCTTGTTAATAAAATGCTTAAGCATTGCt                       >  2:6184148/1‑140 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCAAGCGCGGTTTTGAGAACAAGCCTTTTAAATTCAGCTTGTTAATAAAATGCTTCAGCATTGCt                       >  2:4913319/1‑140 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGCGTCTTTGATTGGTTTTTGCTCAACGGATGGAATTTTCGTCGGGGCGCAAATGAGCCTGCGCGGGTTTGAGAACAATCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCt                       >  1:1947880/1‑140 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGCGTCTTTGATTGCTTTTTTCTCAACGGATGTAATATTCGTCGGGCCGCAAATGAGCATGCGCGGTTGTTTGAACAAGCCTTTTACATTCAGCTTGTTTATAAAATTCTTCATCATTGCt                       >  1:51490/1‑140 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGCGTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCt                       >  2:3893382/1‑140 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTCTGCGGATTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCt                       <  2:6355906/140‑1 (MQ=255)
                                                                                                                    tACCGCCGCTTTTTTATGCGGCTTCTTTGATTGCTTTTTTCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTTAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAAAGCGTCATCATTGCt                       >  2:2850418/1‑140 (MQ=255)
                                                                                                                        gccgcTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTg                   <  2:189080/140‑1 (MQ=255)
                                                                                                                         ccgcTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTGt                  <  2:4372095/140‑1 (MQ=255)
                                                                                                                           gcTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGtttt                                                                          <  1:161440/82‑1 (MQ=255)
                                                                                                                                tttCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATAGTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTGTTACTTCa           <  2:5324826/140‑1 (MQ=255)
                                                                                                                                     gCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTGTTACTTCAAAGTc      <  1:2385990/140‑1 (MQ=255)
                                                                                                                                         cttcttTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTGTTACTTCAAAGTCAGCa  >  2:3452935/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCTTTTTTCTGCGGCTTCTTTGATTGCTTTTTGCTCAACGGATGTAATATTCGTCGGGCAGCAAATGAGCATGCGCGGTTTTGAGAACAAGCCTTTTACATTCAGCTTGTTAATAAAATGCTTCAGCATTGCTTCTGTTACTTCAAAGTCAGCA  >  NZ_CP034943/3569151‑3569427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: