Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,635,976 C→T 100% A174T (GCA→ACA)  EO946_RS18730 ← fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,635,9760CT99.7% 2271.9 / ‑3.7 601A174T (GCA→ACA) EO946_RS18730fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (260/338);  total (263/338)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 627 total aligned reads displayed.

TGTCAGCAGTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGAAA  >  NZ_CP034943/3635841‑3636112
                                                                                                                                       |                                                                                                                                        
tGTCAGCAGTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAgg                                                                                                                                      <  2:1642648/140‑1 (MQ=255)
                 ccAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTgcg                                                                                                                     <  2:1239285/140‑1 (MQ=255)
                 ccAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTgcg                                                                                                                     <  1:6008477/140‑1 (MQ=255)
                  cAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGACCAGAACATAATGCAGGTGTAAGGCAGTCGATGCCCGTgcgc                                                                                                                    >  2:4816964/1‑140 (MQ=255)
                  cAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCGTTGTAAGGGCCGTGACCAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTgcgc                                                                                                                    >  2:4351280/1‑140 (MQ=255)
                   aGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCt                                                                                                                   >  1:7549532/1‑140 (MQ=255)
                   aGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCt                                                                                                                   >  1:5750804/1‑140 (MQ=255)
                       ccgccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTcaac                                                                                                               >  1:209719/1‑140 (MQ=255)
                       ccgccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTcaac                                                                                                               <  1:6809377/140‑1 (MQ=255)
                        cgccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGTGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTcaaca                                                                                                              <  1:2360490/140‑1 (MQ=255)
                         gccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCAGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTcaacaa                                                                                                             <  2:5038020/140‑1 (MQ=255)
                          ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                            >  1:2704441/1‑140 (MQ=255)
                          ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                            >  2:4398534/1‑140 (MQ=255)
                          ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                            >  1:5162064/1‑140 (MQ=255)
                          ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                            >  2:7393401/1‑140 (MQ=255)
                          ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGACAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                            >  1:399424/1‑140 (MQ=255)
                            gTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAgg                                                                                                                                      <  1:4372601/112‑1 (MQ=255)
                                  aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  2:5750330/140‑1 (MQ=255)
                                  aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  1:7105310/140‑1 (MQ=255)
                                  aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  1:335554/140‑1 (MQ=255)
                                  aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  1:3649774/140‑1 (MQ=255)
                                  aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  2:6389798/140‑1 (MQ=255)
                                  aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  1:1020320/140‑1 (MQ=255)
                                  aaCTAGAGGAAGAGCAGTTGTGTTTCGGATCTCTTCCATCTCTTTGAAGCCAATGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                    <  1:4500190/140‑1 (MQ=255)
                                   aCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                   <  2:1822734/140‑1 (MQ=255)
                                   aCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                   <  2:1136412/140‑1 (MQ=255)
                                    cTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                  <  2:4604318/140‑1 (MQ=255)
                                    cTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                  <  1:4191844/140‑1 (MQ=255)
                                     tAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGACCAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAcg                                                                                                 >  2:1320614/1‑139 (MQ=255)
                                         ggAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCttt                                                                                             <  2:2945410/140‑1 (MQ=255)
                                         ggAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCttt                                                                                             <  1:353142/140‑1 (MQ=255)
                                         ggAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCttt                                                                                             <  1:5480210/140‑1 (MQ=255)
                                              aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                        <  1:6675516/140‑1 (MQ=255)
                                              aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                        <  1:7412965/140‑1 (MQ=255)
                                              aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                        <  1:6252630/140‑1 (MQ=255)
                                              aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                        <  2:4097928/140‑1 (MQ=255)
                                              aCCAGTTGTGTGTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                        <  1:6974774/140‑1 (MQ=255)
                                                  gTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCGTGGCACTCTTTAGGGTCAGc                                                                                    >  2:3673335/1‑140 (MQ=255)
                                                  gTTGTGTTTCCGATCTCTTCCATCTCTTAGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTGTAGGGTCAGc                                                                                    >  2:7609689/1‑140 (MQ=255)
                                                   ttGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCg                                                                                   <  2:2491990/140‑1 (MQ=255)
                                                   ttGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCg                                                                                   <  1:1796321/140‑1 (MQ=255)
                                                   ttGTGTTTCCGATCTCGTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCg                                                                                   <  2:4058041/140‑1 (MQ=255)
                                                        tttCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGAt                                                                              >  2:357379/1‑140 (MQ=255)
                                                        tttCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCGTGGCACTCTTTAGGGTCAGCGTAGAt                                                                              >  1:6410458/1‑140 (MQ=255)
                                                        tttCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCGTGGCACTCTGTATGGTCAGCGTAGAt                                                                              >  1:1351966/1‑140 (MQ=255)
                                                               tctcTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCt                                                                       <  2:6548693/140‑1 (MQ=255)
                                                                ctctTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCtt                                                                      <  2:6274399/140‑1 (MQ=255)
                                                                ctctTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCtt                                                                      <  1:4581875/140‑1 (MQ=255)
                                                                ctctTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCtt                                                                      <  2:1982811/140‑1 (MQ=255)
                                                                ctctTACATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCtt                                                                      <  1:1710263/140‑1 (MQ=255)
                                                                  ctTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCa                                                                    >  2:4935080/1‑140 (MQ=255)
                                                                           ctTTGAAGCCAAGGTTTGGCTCACCTGTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACg                                                           <  2:5825264/140‑1 (MQ=255)
                                                                             ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTgcgc                                                                                                                    <  1:4783539/81‑1 (MQ=255)
                                                                             ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGTGCTCAACAAGCTCTTGGc                                                                                                    <  2:7447585/97‑1 (MQ=255)
                                                                             ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTc                                                         >  2:3901617/1‑140 (MQ=255)
                                                                             ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTc                                                         >  2:4733735/1‑140 (MQ=255)
                                                                              tGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCa                                                        >  2:5329622/1‑140 (MQ=255)
                                                                                      aGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCACTAACGTCATCTTCTTg                                                >  2:7200001/1‑140 (MQ=255)
                                                                                      aGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTg                                                >  2:6650553/1‑140 (MQ=255)
                                                                                      aGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAAAAAGCTCTTGGCACGCGTTAGGGTCAGCGTAGATTACGCCTGCAGCCATAACGTCATCTTCTTg                                                >  2:6853480/1‑140 (MQ=255)
                                                                                           tGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTAAGGGTCAGCGTAGATTACGCCTTAAGCACTAACGTCATCTTCTAGTccgc                                           >  2:213379/1‑140 (MQ=255)
                                                                                                  ccTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCAtcttct                                                  <  1:4874044/126‑1 (MQ=255)
                                                                                                   cTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGtt                                   >  1:906431/1‑140 (MQ=255)
                                                                                                      tGTAAGGGCCGTGAACAGAACCTAATGCAGGGGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCa                                >  1:3730663/1‑140 (MQ=255)
                                                                                                        tAAGGGCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAg                              >  1:319321/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGa                                        <  2:2610124/125‑1 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGa                                        >  1:2610124/1‑125 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGa                                        <  2:1431763/125‑1 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTcc                                 >  1:2445525/1‑132 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         >  2:7667323/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         <  1:1956450/140‑1 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         >  1:2738537/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         >  1:2993091/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         >  1:5873902/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         >  1:7229068/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         <  2:6994161/140‑1 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAACCTCAg                         >  1:7310444/1‑140 (MQ=255)
                                                                                                             gCCGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCGTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                         >  1:3835323/1‑140 (MQ=255)
                                                                                                               cGTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCt                       <  1:4268058/140‑1 (MQ=255)
                                                                                                                gTGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCtt                      >  2:2754944/1‑140 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  1:1258179/140‑1 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  1:6596182/140‑1 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  2:5226388/140‑1 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  2:558154/140‑1 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  2:5941302/140‑1 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  1:1476518/140‑1 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCGTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     >  2:3149229/1‑140 (MQ=255)
                                                                                                                 tGAACAGAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCGTGAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                     <  1:3250679/140‑1 (MQ=255)
                                                                                                                      aGAACCTAATGCAGGTGTATGGCAGTCGATGCCCGTGCGCTCAACACGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTGGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAg                <  2:61057/140‑1 (MQ=255)
                                                                                                                       gAACCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGa               >  1:5211991/1‑140 (MQ=255)
                                                                                                                        aaCCTAATGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGAt              >  1:6898221/1‑140 (MQ=255)
                                                                                                                             aaTGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTcc         >  2:621271/1‑140 (MQ=255)
                                                                                                                             aaTGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTcc         >  2:3351265/1‑140 (MQ=255)
                                                                                                                             aaTGCAGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTcc         >  1:840269/1‑140 (MQ=255)
                                                                                                                                  aGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGa    <  2:840269/140‑1 (MQ=255)
                                                                                                                                  aGGTGTAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGa    <  1:5003351/140‑1 (MQ=255)
                                                                                                                                    gtgtAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa  >  2:3224730/1‑140 (MQ=255)
                                                                                                                                    gtgtAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa  >  2:2396709/1‑140 (MQ=255)
                                                                                                                                    gtgtAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGGAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa  >  1:1192278/1‑140 (MQ=255)
                                                                                                                                    gTGCAAGGCAGTCGATGCCAGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGATCAGCGTAGATTACGCCTTCAGCGATAACGTCATCTTCTTGTCCGCCTACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa  >  1:1567184/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TGTCAGCAGTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGAAA  >  NZ_CP034943/3635841‑3636112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: