Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,719,864 T→G 7.6% S79R (AGC→CGC)  EO946_RS19145 ← YwdI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,719,8640TG 7.6% 1356.9 / 15.2 621S79R (AGC→CGC) EO946_RS19145YwdI family protein
Reads supporting (aligned to +/- strand):  ref base T (268/272);  new base G (7/74);  total (275/346)
Fisher's exact test for biased strand distribution p-value = 1.29e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 632 total aligned reads displayed.

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001
                                                                                                                                           |                                                                                                                                         
    cTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTccc                                                                                                                                       <  2:251330/140‑1 (MQ=255)
     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  2:4676223/1‑140 (MQ=255)
      gTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGGTTAATCCCGCGCTTCCcat                                                                                                                                     >  2:4477160/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  2:2850861/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:6621429/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:7617376/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:2377144/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCACCGGCTTTCGATATTTGTTTAATCCCGCGCTTCCaatc                                                                                                                                    >  2:2314304/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                               >  1:1671965/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACAGAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCATGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                               >  2:3592369/1‑140 (MQ=255)
              cAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttttt                                                                                                                             <  1:7512696/140‑1 (MQ=255)
               aGCGCACCAAGAACCACTTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACGCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTGCCCATCATTTTTTc                                                                                                                            <  2:3991974/140‑1 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:6313049/1‑140 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:5799084/1‑140 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        <  1:462645/140‑1 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  2:2935751/1‑140 (MQ=255)
                        aGAACCAAGTTCACATAGCTCCACCTTTCTCATTAAAAATCAAATACAGCCTCTCCGTGTCCGTCCTCTTCCTGCTTCTCTGTCTCCCGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCCTCAg                                                                                                                   <  1:3431935/140‑1 (MQ=255)
                         gAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGc                                                                                                                  <  2:7044062/140‑1 (MQ=255)
                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  2:6621374/140‑1 (MQ=255)
                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  2:1933063/140‑1 (MQ=255)
                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  2:1105215/140‑1 (MQ=255)
                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  1:7158952/140‑1 (MQ=255)
                             cAATTTCACAGAGCTCAACCTTTCTCAATAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  2:7207420/140‑1 (MQ=255)
                                       tAGCTCAACCTTTCTCATTATAAATCAAATATAGTCTCTCGTTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCTCTGCCATTCATTTTTTCGATCATCAGCTGGTCTGTCGATgt                                                                                                    <  2:7510343/140‑2 (MQ=255)
                                       tAGCTCAACCTTTCTCATTAAAAATCAAATACAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATGATTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  2:1539560/140‑1 (MQ=255)
                                       tAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    >  2:5607170/1‑140 (MQ=255)
                                       tAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  2:5840927/140‑1 (MQ=255)
                                       gaGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  2:5150550/139‑1 (MQ=255)
                                        aGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGc                                                                                                   >  1:2170056/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  1:1484982/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  1:5676358/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 <  2:6941344/140‑1 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTGTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  1:3522300/1‑140 (MQ=255)
                                                cTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGc                                                                                           <  1:5529685/140‑1 (MQ=255)
                                                cTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGc                                                                                           <  2:6848282/140‑1 (MQ=255)
                                                cTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCGGGTCTGTCGATGGCGGTGCGGc                                                                                           <  1:931882/140‑1 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:2765160/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:6723945/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:2083615/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:4040593/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  2:5463581/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATACCGCGCTGCCCATAATTTTTTCCATCATCATCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:3124938/1‑140 (MQ=255)
                                                      cATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACt                                                                                     <  2:3502137/140‑1 (MQ=255)
                                                        ttAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCg                                                                                   <  1:1154506/140‑1 (MQ=255)
                                                         tAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCgg                                                                                  >  2:3109122/1‑140 (MQ=255)
                                                           aaaaTCAAATAAAGCCTCTCCGGTTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:422942/140‑1 (MQ=255)
                                                           aaaaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                >  2:6388618/1‑140 (MQ=255)
                                                           aaaaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:6439742/140‑1 (MQ=255)
                                                           aaaaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:5306556/140‑1 (MQ=255)
                                                           aaaaTCAAATAAAGACTCTCCGTTTCCGTCCACTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  2:7661631/140‑1 (MQ=255)
                                                           aaaaTCAAAGAAAGACTCTCCGTTTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:3043033/140‑1 (MQ=255)
                                                             aaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  1:374957/140‑1 (MQ=255)
                                                                  aaTACAGACTCTCCGTGTCCGTCCTCTTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGGTTACTCCCGCGCGTCCCATCATTCTTTCCCTCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:6077257/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:1760174/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2912207/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:2168691/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:5708448/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2939412/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:1019065/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:4945258/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:5744312/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:6727400/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATGTTTTCCATCCTCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  2:6481498/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  2:4200474/140‑1 (MQ=255)
                                                                            ctcCGTTTCCGTCCTATTCATGCTTCTCTTTCTCCTTCTTTCGATATTTGTTTAATCCCGAGATTCCCATCATTTTTTCCATAATCAGCTTTTCTGTCGATGGCGGTGCGGCAGGACTCGGCTTTCTGCTCGGATGTATg                                                               >  1:4927391/1‑140 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:3335290/129‑1 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  2:2118124/1‑140 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  2:1260788/1‑140 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTGTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  1:2703088/1‑140 (MQ=255)
                                                                                 tttCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCgggg                                                          <  1:3947443/140‑1 (MQ=255)
                                                                                   tCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGgcg                                                        <  2:4333353/140‑1 (MQ=255)
                                                                                       tCCTCTTCGTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGAAGGACTCGGCGTGCTGCTCGGCTCTATGAGGGGCGCGCt                                                    >  2:4824655/1‑140 (MQ=255)
                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:5005885/1‑140 (MQ=255)
                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  2:622237/1‑140 (MQ=255)
                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  2:2908708/1‑140 (MQ=255)
                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:6874022/1‑140 (MQ=255)
                                                                                        ccTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTc                                                   >  1:7344074/1‑140 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTGCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:5334479/140‑1 (MQ=255)
                                                                                            ttCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  1:5090526/137‑1 (MQ=255)
                                                                                            ttCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCgg                                               >  2:3157426/1‑140 (MQ=255)
                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  2:1388409/140‑1 (MQ=255)
                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  2:840452/140‑1 (MQ=255)
                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGCCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  2:5991713/140‑1 (MQ=255)
                                                                                                    tctcTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCCTCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  2:7367039/140‑1 (MQ=255)
                                                                                                     ctctTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCt                                      <  1:243920/140‑1 (MQ=255)
                                                                                                        tttCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:6013095/136‑1 (MQ=255)
                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                           >  1:1306873/1‑140 (MQ=255)
                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                           <  2:2765160/140‑1 (MQ=255)
                                                                                                                    tCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         >  1:7422054/1‑90 (MQ=255)
                                                                                                                               ttAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGaa            <  2:4070961/140‑1 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCCTCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:156726/140‑1 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  2:3893725/140‑1 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  2:3737381/140‑1 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGAGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:3845858/140‑1 (MQ=255)
                                                                                                                                    cccGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  2:3265888/140‑1 (MQ=255)
                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:4375311/1‑140 (MQ=255)
                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:5459300/1‑140 (MQ=255)
                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:837806/1‑140 (MQ=255)
                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGAGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:59473/1‑140 (MQ=255)
                                                                                                                                         gcTGCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCg  <  1:6192879/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: