BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,962	C→A	5.2%	D36Y (GAT→TAT)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,962	0	C	A	5.2%	1096.2 / 8.1	418	D36Y (GAT→TAT)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (179/197); new base A (34/3); total (218/200)
Fisher's exact test for biased strand distribution p-value = 5.71e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTATT  >  NZ_CP034943/3746823‑3747101

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                                                                             <  1:5057805/140‑1 (MQ=255)

  gtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTg                                                                                                                                           <  1:4049448/140‑1 (MQ=255)

     tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                                                                        <  1:4674067/140‑1 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTTTTTATGGAACACCACCAAATTTTCCCATTTTCACCGCCCACGGAAAAACAAGCCCCCCCGTAAAATATGTAttttatac                                                                                                                                 >  1:6802883/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTATTCAACAGCAGCATATGTACCCAATGTGAGCGCGCACGGTAACACATGCCCCCCCTTAAAATCTGTATTTTATcc                                                                                                                                 >  2:5960402/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTTTTCAAAAACAGCAAAATTAACCAATGTAAGCGCGCCCCGCAAAACAAACCCCCCCGTATAAACAGTATTTtctgc                                                                                                                                 >  1:2362081/1‑138 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAACATCAGCATATTTACACATTGTCAGCCCGCACGGAAAAACATGGCCGCCCGTATAATAAGTAAGTTCTcc                                                                                                                                 >  2:5624642/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCAAATTTACCCAATGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTcc                                                                                                                                 >  2:2377930/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTAACCAATTTAAGCGCCCACGGAAACAAAATCCCCCCGTTATAATCTTTAAGTTATcc                                                                                                                                 >  2:7579939/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCACCATATGTAACCATTGTCAGCGCGCACCGAAAAACAAGACCGCCCGTATAATCTGTATGTTATcc                                                                                                                                 >  2:4027670/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAAAACCCCCAAATGTGCCCATGGTCAGCCCGCACCGAAACAACTGCCCGCCCGTATAATAAGTGTGttggac                                                                                                                                 >  1:716686/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAAAACCAGCATATGTAACCATTGTGAGCGCGCACGGAAACAAAAGCCCGCCGTTTTAATATGTATGttgtgc                                                                                                                                 >  1:5531813/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTATGCAACAGCAGCAAAAGTACCCATTGTCAGCGCGCACGGAAACACATGCCCCCCGGTATAATAGGTATGTTGTcc                                                                                                                                 >  2:4749903/1‑140 (MQ=255)

                       cccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCa                                                                                                                      <  2:6578921/140‑1 (MQ=255)

                        ccTACGTCTTTAAAGTTGTCTGCATACATCCGGACAACCCCGGCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                     <  2:6935720/140‑1 (MQ=255)

                        ccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                     <  2:4328051/140‑1 (MQ=255)

                        ccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                     <  2:6655437/140‑1 (MQ=255)

                        ccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                     <  2:5652614/140‑1 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTATGCCACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAAGCCCGCCGGTATAATCGGTTTGTTCGCCAATCAGATTCActct                                                                                                                  >  1:7649115/1‑140 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGGTTGTTCCACAGCCCCAAATTTACCCCAAGGAAGCGCCCACCGCAACCCCTGCCCGCCCGTAAAATCGGTATGGTCTACAATCAGAttacagct                                                                                                                  >  1:6366856/1‑134 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAACATCAGCATAAGTAACCCTTGTCAGCCCGCACCGAAACACATGCCCCCCGGTATAATCTGGATGTTCTGCCATCAGAttgaatgt                                                                                                                  >  1:5657181/1‑134 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTATTCAAAACCAGCATATTTTCCCCATGTCAGCGCGCACCGACACACAAGCCCGCCGTTATAATCTGTATGTTGTCCAATAAGATTGActct                                                                                                                  >  1:6142254/1‑140 (MQ=255)

                                   aaaGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTg                                                                                                          <  2:6142254/140‑1 (MQ=255)

                                          tCTGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAACAGCAGCAAATTTACCCATAGTCAGCGCGCACCGAAACAAAAGCCCGCCGGTATAAAATGGATCTTATACAATAAGATTGAATATTCACGGTGCGaaaaa                                                                                                   >  2:7241994/1‑140 (MQ=255)

                                                  cATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTa                                                                                           <  2:7119874/140‑1 (MQ=255)

                                                   aTCCGGACAACCCCGTCGTTTCTTTATTCAACAGCAGCATATGTACCCATTGTGAGCGCGCACGGAAACACATGGCCGCCCTTATAATCTGTATGTTCTACCATCAGATTACATCGTACAGGTGCGAAAAAAAAACGTaa                                                                                          >  2:7360137/1‑140 (MQ=255)

                                                      cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAccc                                                                                       <  2:2009870/140‑1 (MQ=255)

                                                      cGGAAAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAccc                                                                                       <  2:5493131/140‑1 (MQ=255)

                                                        gACAACCCCGTCGTTTGTTTATGCAACAGCACCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCCCCGTTATAAACTGTATGTTATCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAAACCTAAccccg                                                                                     >  2:4818652/1‑139 (MQ=255)

                                                           aaCCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCtt                                                                                  <  1:6484404/140‑1 (MQ=255)

                                                             ccccGTCGTTTATTTCTGCAACAGCAGCAAATGGAACCATTGTCAGCGCGCACGGCAACACATGCCCGCCGGTTTAATCTGTATGTTCTCCAATCAGATTAACACTTCCACGTGCGAAAAAAAACCGTACACCctcggtt                                                                                >  2:2149945/1‑136 (MQ=255)

                                                               ccGTCGTTTATTTCTGCAACAGAAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCATTCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtc                                                                              <  2:5165941/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct                                                                             <  2:1303045/140‑1 (MQ=255)

                                                                     tttctttcTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGaa                                                                        <  2:3548956/140‑1 (MQ=255)

                                                                       tctttctGCAACATCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                      <  1:1176557/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGTACCCAGTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCACTCAGATTCACTCGTCCAGGGGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                      <  1:1012117/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGTAACCATTTTCATCGCGCACCGTAAAACATTCCCGCCCTTAAAAACATTATGTTGTGCAAAGAGAATCACTCTTACAAGTTCGAAAAAAAACCGTAACCCCGCTTTTTATCCGAATa                                                                      >  2:5381019/1‑140 (MQ=255)

                                                                             tGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                                                                <  1:2698280/140‑1 (MQ=255)

                                                                             tGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGGTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                                                                <  2:379731/140‑1 (MQ=255)

                                                                               cAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGACACACATGCCCGCCGTAATAATCGGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAAACGTAACCGCTCTTTTTCTCCGCATACACtagag                                                              >  2:7692846/1‑136 (MQ=255)

                                                                                   agcagcATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCTTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          <  2:4127609/140‑1 (MQ=255)

                                                                                   agcagcATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          <  2:7461036/140‑1 (MQ=255)

                                                                                   agcagcATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          >  2:1621854/1‑140 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:3966035/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        >  2:4985987/1‑140 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:2974264/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:2779942/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:272604/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:1889673/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:1703014/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:1238449/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:4450290/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACATGACCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTGTTCTCCGAATACACTTGCAAATATc                                                        <  2:2869524/140‑1 (MQ=255)

                                                                                         ctatGTACCCATTGTCAGCGCGCACTGAAACACCTGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCCGGTGCGAAAAAAAACCGGAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt                                                    <  1:6961882/139‑1 (MQ=255)

                                                                                         atatGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt                                                    <  2:5604366/140‑1 (MQ=255)

                                                                                             ggaCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACACCTCTTTTTCTCCGAATACACATGCAAATATCCACTTTAg                                                >  2:6332523/3‑140 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATACTCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCGTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  2:5107864/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  2:1055672/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  1:5201253/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  1:3800517/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  1:4172864/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATAACCCCTTTAg                                                >  2:3576791/1‑140 (MQ=255)

                                                                                               aCCCATTGTCAGCGCGCACGGAACCACATGCCCGCCGTTATAATCTGTATTTTCTCCAATCAGATTCACTCTTCCTGGTGCGAAAAAAAACCGTACCCCCTCGTTTTCTCCGATTACAGTTGCAAATTTCCCCTTTAGct                                              >  1:7281384/1‑140 (MQ=255)

                                                                                               aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                              >  1:817763/1‑140 (MQ=255)

                                                                                                cccATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctc                                             >  2:1761753/1‑140 (MQ=255)

                                                                                                cccATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctc                                             >  2:1486262/1‑140 (MQ=255)

                                                                                                  cATTTTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                           <  2:257903/140‑1 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:5143495/127‑1 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTATCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                           >  1:3628527/1‑140 (MQ=255)

                                                                                                   aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtc                                                                              >  2:6245194/1‑104 (MQ=255)

                                                                                                   aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtc                                                                              <  1:6245194/104‑1 (MQ=255)

                                                                                                    ttGTTAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                         <  1:1407594/140‑1 (MQ=255)

                                                                                                    ttGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                         <  2:5840060/140‑1 (MQ=255)

                                                                                                    ttGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                         >  1:2899127/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACCTGCCCGCCGTTATAATCTGTCTGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTGGCAAATATCCCCTTTAGCTCTTccc                                        >  1:3453287/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:1634430/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:2547222/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:5834857/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:5893010/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:5339181/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:93932/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:4873908/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGATCTTacc                                        >  1:4869808/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTGTTTCGCCGAATACCCTTGCATATATCCGCTTTAGCTCTTccc                                        >  2:4613307/1‑140 (MQ=255)

                                                                                                        cAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                     >  1:3145071/1‑140 (MQ=255)

                                                                                                        cAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                     <  1:2157072/140‑1 (MQ=255)

                                                                                                        cAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                     <  1:2027898/140‑1 (MQ=255)

                                                                                                           cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaa                                                                                                    <  2:1212194/74‑1 (MQ=255)

                                                                                                             cgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGc                                >  2:5894661/1‑140 (MQ=255)

                                                                                                               cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCACTCAGATTCACTCTTCCAGGTGCGAAAATAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              >  2:2276574/1‑140 (MQ=255)

                                                                                                               cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              >  2:1429990/1‑140 (MQ=255)

                                                                                                                aCGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAAt                             <  1:2382059/140‑1 (MQ=255)

                                                                                                                aCGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAAt                             <  1:3364720/140‑1 (MQ=255)

                                                                                                                              ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTGCATTGtt               >  2:3246169/1‑140 (MQ=255)

                                                                                                                                  cgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        >  1:3536813/1‑95 (MQ=255)

                                                                                                                                  cgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCACTCACCTGTCAGTGttttat           >  1:4332190/1‑140 (MQ=255)

                                                                                                                                  cgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCAGTGttttat           >  2:3504993/1‑140 (MQ=255)

                                                                                                                                      ataATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttattttt       <  2:4406166/140‑1 (MQ=255)

                                                                                                                                          tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:7504912/140‑1 (MQ=255)

                                                                                                                                          tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  2:877204/140‑1 (MQ=255)

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTATT  >  NZ_CP034943/3746823‑3747101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`