BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,945,908	T→G	6.6%	L388R (CTT→CGT)	EO946_RS20305 →	Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,945,908	0	T	G	6.6%	1208.3 / 4.9	529	L388R (CTT→CGT)	EO946_RS20305	Na+/H+ antiporter
Reads supporting (aligned to +/- strand): ref base T (243/201); new base G (66/2); total (326/203)
Fisher's exact test for biased strand distribution p-value = 8.44e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 570 total aligned reads displayed.

CCGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTC  >  NZ_CP034943/3945769‑3946047

ccGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCt                                                                                                                                             <  1:326019/140‑1 (MQ=255)

  ggTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttg                                                                                                                                           <  1:1012390/140‑1 (MQ=255)

  ggTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttg                                                                                                                                           <  1:964648/140‑1 (MQ=255)

  ggTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttg                                                                                                                                           <  2:3362496/140‑1 (MQ=255)

  ggTGACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttg                                                                                                                                           <  2:4158381/140‑1 (MQ=255)

          ggAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAg                                                                                                                                   <  2:4779570/140‑1 (MQ=255)

              gCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTGTTGTTCAGGGGc                                                                                                                               >  1:2024613/1‑140 (MQ=255)

               cATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  2:2063722/140‑1 (MQ=255)

               cATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  1:5196712/140‑1 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCa                                                                                                                            >  2:1953754/1‑140 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCa                                                                                                                            >  2:6009389/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCAc                                                                                                                           >  2:4625696/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCAc                                                                                                                           >  2:6748022/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCCGGGGCTAAc                                                                                                                           >  1:4353380/1‑140 (MQ=255)

                      tCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCATGGGCTCACCAta                                                                                                                       >  2:4935321/1‑139 (MQ=255)

                         aTTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAg                                                                                                                    >  2:2640324/1‑140 (MQ=255)

                            ggggCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTTTTGTTGCGGGGCTCACCAACAAGCCg                                                                                                                 >  1:6025122/1‑140 (MQ=255)

                              ggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTGTGTTTTTCCTTGTTTTTCACGGGCTCACCATCAAGCCGct                                                                                                               >  2:6410516/1‑140 (MQ=255)

                              ggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTGAGGGGCTCACCATCAATCCGcg                                                                                                               >  1:2526840/1‑139 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAATACATTCTGTTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTTTTGTTGAGGGGCTCACCATCAATCCGcatatt                                                                                                           >  2:1304625/1‑135 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGcttctt                                                                                                           >  2:7167517/1‑140 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTGCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCACGTTTTTTTCAGGGGCAAACCATCAATCCGcgtctt                                                                                                           >  1:6268826/1‑140 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:7074796/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:167283/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  2:762135/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:2837161/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  2:4693388/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  2:3504016/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:1636750/140‑1 (MQ=255)

                                     tGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGATACTTCCCGGGACGTGAAGACATTCTTGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTTaa                                                                                                        <  2:3020544/140‑1 (MQ=255)

                                          ggTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGtt                                                                                                   <  1:3335990/140‑1 (MQ=255)

                                          ggTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGtt                                                                                                   <  1:74874/140‑1 (MQ=255)

                                            ttCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  1:4141818/111‑1 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTGTTGTTGAGGGGCTAACCATCAACCCGCATCTTGAAGGGGTAgg                                                                                                >  1:558619/1‑140 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:7025941/131‑1 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTCAGGGGCTCAACATAAAGCCGCTTCTTGAACGGTTatg                                                                                                >  2:7031550/1‑138 (MQ=255)

                                              ctctGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTGTTGTTAAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGgt                                                                                               >  2:4050369/1‑140 (MQ=255)

                                              ctctGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTTTTCAGGGGCTCACCAACAAGCCCCGTGTTGAACGGGTAGgt                                                                                               >  1:153469/1‑140 (MQ=255)

                                              ctctGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTTTTAAGGGGCTCACCATCAAGCCGCGTATTGAACGGTTAGgt                                                                                               >  1:297759/1‑140 (MQ=255)

                                               tctGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTACCGTGTTGTTCAGGGGCTCAACATCAAGCCGCGTCTTGAAAGGGTAGgtg                                                                                              >  1:3118915/1‑140 (MQ=255)

                                                 tGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTAAGGGGCTCACCATCAAGCCGCTTCTTGAAAGGTTAGGtgtg                                                                                            >  2:792122/1‑140 (MQ=255)

                                                   tCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTGTTTTTGAGGGGCTCACCATCAAGCCCCTTGTTGAACGGTTAGGTGTGaa                                                                                          >  1:727597/1‑140 (MQ=255)

                                                    ccATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGAACGGTTAGGGGTGTAt                                                                                         >  1:5701026/1‑140 (MQ=255)

                                                      aTTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTAACGTGTTGTTAAGGGGCTAAACATAAATCCGCTTCTTGAACGGTTAGGTGTGAATCa                                                                                       >  2:839352/1‑140 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaaa                                                                                     <  1:7211165/140‑1 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGACTAGAGAATTCACGGGACGTGAAGACATACTGGTTTTAGCCTTCAGTGTCGTTTTTTTTTACCATGTTGTTGAGGGGATGAACAACAAGCCGCGTCTTGAACAGTTATCTGTGaaaaaaa                                                                                     >  1:4921401/1‑135 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTCGTTTTTCAGGGGCTCACCATCAAGCCGCGTCTTGGACGGTTAGGTGTGAATCaaaaaaaa                                                                                >  1:3538413/1‑140 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCa                                                                                                                      <  1:7395489/102‑1 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCa                                                                                                                      >  2:7395489/1‑102 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGGTGTTCAGGGGCTCCCCAGCAAGCCGCTTCGTGAACGGTTTGGTGTGAATCAAAaagac                                                                                >  1:7567974/1‑139 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGGTGTTGAGGGGCTCACCATCAAGCCGCGTCGTGAACGGGTAGGGGTGAATCAAAaagaa                                                                                >  1:1174183/1‑140 (MQ=255)

                                                               gtggtgAGCCGGCCTAGAGACTTCCCGGGACGTGAATACATTCTGGTTTTCGCCTTCAGTGTGGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaaga                                                                              <  1:6410516/140‑1 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATAAAGCCGCTTCTTGAACGGTTAGGGGTGAATCAAAaagaaga                                                                              >  2:664387/1‑140 (MQ=255)

                                                               gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTGAGGTGTGAATCAAAaagaaga                                                                              >  2:2253863/1‑140 (MQ=255)

                                                                 gCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaagaag                                                                            <  1:1311505/140‑1 (MQ=255)

                                                                 gCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATAAAAaagaagaag                                                                            <  1:4956158/140‑1 (MQ=255)

                                                                       cctgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGtt                                                                                                   <  1:5857595/111‑1 (MQ=255)

                                                                       cctgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAAcc                                                                      >  2:5787759/1‑140 (MQ=255)

                                                                         tgtctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    <  1:773777/137‑1 (MQ=255)

                                                                         tgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         >  2:4366707/1‑103 (MQ=255)

                                                                         tgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    <  2:5218275/140‑1 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  1:4501698/81‑1 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCTCCTTCATTGTCGTTTTTTTTTCCCTTGTTGTTCAGGGGCGCACCATCAAGCCGCTTATTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                  <  1:6824585/140‑1 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTGTTGTTCAGGGGCTCACCAACAAGCCGCGTCTTTAACGGTTAGGGGTGAATAAAAAATAATAAGTGAACCAGGa                                                                  >  1:1866671/1‑140 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCCGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                  >  2:1450807/1‑140 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAATCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                  >  2:5410633/1‑140 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCACCCAGGa                                                                  >  2:2451474/1‑140 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    <  1:2423361/138‑1 (MQ=255)

                                                                             tAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGt                                                                <  2:3831090/140‑1 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCGTGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTTAGCGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGa                                                         >  2:526923/1‑140 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAACCCGCGTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGa                                                         >  2:3312543/1‑140 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGGTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTTGGTGTGAATCAAAAAGAAGCAGGCAACCCGGCGTaggaggc                                                         >  2:6097562/1‑139 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTTTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTTAACGGTTAGGTGTGAATGAAAAATAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  2:651951/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         >  2:7250901/1‑91 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaa                                                                                      <  1:5431592/110‑1 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATAAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  2:4429645/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTGAGGGGCTCACCAACAACCCGCGTCGGTAACGGGTTGGGGTGAATAAAAAAGAATAAGGCAACCAGGAGTAAGATGaa                                                        >  1:1921465/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCACCCTAAACCCGCTTGTTGGACGGGTAGGGGTGAATCAAAAAGAAGAAGGCAACCAGGACTATGAGGaa                                                        >  1:6474024/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCGTGTCCGGGTAGGTGTGAATCAAAAAGAAGAAGGCAACCCGGAGTATGAGGaa                                                        >  2:3955576/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCGTGAACGGGTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                        >  2:3623686/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACAGTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGGTGTTAAGGGGCTCACCATCAAGCCGCGTCTTGAACGGTTCGGTGTTAATCAAAAAGGAGAAGGCAACCAGGAGGATGAGGaa                                                        >  2:510566/1‑140 (MQ=255)

                                                                                      cccGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTTTTTTTGAGGGGCTAAACATAAAGCCGCGTCTTGAACGGTTAGGGGTGAATAAAAAAGAAGAAGGCAAACACGAGTATTATGaaa                                                       >  1:3891828/1‑139 (MQ=255)

                                                                                      cccGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTTTTAAGGGGCTCACCATCAAGCCGCTTCGTGAACGGTTAGGTGTGAATAAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAt                                                       >  1:3634018/1‑140 (MQ=255)

                                                                                      cccGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGATTTGTTTTCCCTTGTTGTTCAGGGGCTGACCATCATGCCGCATCGTGAACGGTTAGGTGTGAGTCCAAAAGAAGAACTCACGCAGGAGGAAGAGGAAt                                                       >  1:2676613/1‑140 (MQ=255)

                                                                                            aCGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgcttg                                                 <  1:4044588/140‑1 (MQ=255)

                                                                                                 aaGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTGAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTAGGAGGAATTGCTTGCaaaa                                            >  2:6222947/1‑140 (MQ=255)

                                                                                                 aaGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGGTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCaaaa                                            >  2:5475052/1‑140 (MQ=255)

                                                                                                     cATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGAc                                        <  2:1722402/140‑1 (MQ=255)

                                                                                                      aTTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTTTTGTTCAGGGGCTCACCAACAAGCCGCTTCGTGAACCGTTAGGTGTGAATCAAAAAGAAGAAGGCAAACAGGAGTATGAGGAATTTCTTGAAAAAGGaaa                                       >  2:3174647/1‑138 (MQ=255)

                                                                                                       ttCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACAt                                      <  2:4968828/140‑1 (MQ=255)

                                                                                                       ttCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACAt                                      <  2:3076233/140‑1 (MQ=255)

                                                                                                       ttCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACAt                                      <  1:6708520/140‑1 (MQ=255)

                                                                                                       ttCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGATTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACAt                                      <  2:7555596/140‑1 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATc                                     >  2:5739515/1‑140 (MQ=255)

                                                                                                               ttCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGa                              >  2:1523576/1‑140 (MQ=255)

                                                                                                                 cGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGt                                                                                                    <  1:6092512/68‑1 (MQ=255)

                                                                                                                  gCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAAc                           <  2:2756299/140‑1 (MQ=255)

                                                                                                                           gtCGTTTTGGTTTCCCTTGTTGTTCAGGGGCTCACCATCACTCCGCTTCTTGAACGGTTAGGTGTGACTCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAGTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCaa                  <  2:1897419/140‑1 (MQ=255)

                                                                                                                              gttttgttttCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAaaga               <  1:2170128/140‑1 (MQ=255)

                                                                                                                                         ccTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgct                                                   <  1:4715989/93‑1 (MQ=255)

CCGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTC  >  NZ_CP034943/3945769‑3946047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`