Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 44,865 T→A 21.0% intergenic (‑46/‑455) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8650TA21.0% 418.8 / 107.6 176intergenic (‑46/‑455)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (61/78);  new base A (14/23);  total (75/101)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TTCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAA  >  NZ_CP034943/44730‑44988
                                                                                                                                       |                                                                                                                           
ttCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTttctt                                                                                                                         <  2:3443161/140‑1 (MQ=255)
   tGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGt                                                                                                                      <  1:4358807/140‑1 (MQ=255)
     cGATTCCAAGACTACGACGCAATTCGATAGGAAGAACGACACGTCCTAGCTCATCAACTTGACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCa                                                                                                                    <  2:3318001/140‑1 (MQ=255)
               agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                          <  1:2805091/140‑1 (MQ=255)
               agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                          <  2:2515735/140‑1 (MQ=255)
               agTACGACGCAATTCGATAGGAAGAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAACCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                          <  2:406735/140‑1 (MQ=255)
                gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTcaacaa                                                                                                         <  1:1463565/140‑1 (MQ=255)
                gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACaa                                                                                                         <  2:1270663/140‑1 (MQ=255)
                 tACGACGCAATTCGATACGAATAACCCCCCGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCACCCCAGAGATGATTATTTGTTTAAATTATATATTTCGTCGACATTATTCAACaaa                                                                                                        <  2:3730617/140‑1 (MQ=255)
                  acgacgCAATTTGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACaaaa                                                                                                       >  1:3287539/1‑140 (MQ=255)
                  acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTGTAAATTATATTTTTCTTCGTCATTATTCAACaaaa                                                                                                       >  1:2917085/1‑140 (MQ=255)
                      cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   >  2:1347387/1‑140 (MQ=255)
                      cgCAATTCGATAGGAATAACTACACGTCCTAGCTCAACAACTTTACGTACACTACCCGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   <  1:1077863/140‑1 (MQ=255)
                       gCAATTCGATAGGACTAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCCACAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                  <  2:1142330/140‑1 (MQ=255)
                       gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACGTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                  <  1:5200887/140‑1 (MQ=255)
                         aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                >  1:4115949/1‑140 (MQ=255)
                         aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                <  1:3692386/140‑1 (MQ=255)
                         aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                <  1:1777872/140‑1 (MQ=255)
                         aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAc                                                                                                >  1:5230790/1‑140 (MQ=255)
                         aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAc                                                                                                >  1:3334217/1‑140 (MQ=255)
                              gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                           <  2:1001781/140‑1 (MQ=255)
                              gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                           <  2:4686257/140‑1 (MQ=255)
                                   gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   <  2:1933964/127‑1 (MQ=255)
                                   gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   >  1:1933964/1‑127 (MQ=255)
                                   gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCa                                                                                      >  1:1433192/1‑140 (MQ=255)
                                            cacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGt                                                                             >  2:804289/1‑140 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  >  2:1662517/1‑134 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  >  2:1835266/1‑134 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  >  2:4274950/1‑134 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  <  1:4274950/134‑1 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  1:5075648/1‑140 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:1567172/1‑140 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:3603104/1‑140 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:1604141/1‑140 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:587669/1‑140 (MQ=255)
                                             acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCACCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  >  2:2733668/1‑134 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCTACAAAATCCTTACCTTCATAGCATTAGAAGGttt                                                                           >  2:2334116/1‑140 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  1:166610/1‑140 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  1:1540472/1‑140 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  2:213080/1‑140 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  1:1994814/1‑140 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  2:1296198/1‑140 (MQ=255)
                                              cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTAATAGCATAACAAGGttt                                                                           >  2:3703990/1‑140 (MQ=255)
                                                 ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                        >  2:4401469/1‑140 (MQ=255)
                                                         atcaACTTTACGTACAATACCAGTAGATTTCGTAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:3487153/1‑140 (MQ=255)
                                                         atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:3171776/1‑140 (MQ=255)
                                                         atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:5204472/1‑140 (MQ=255)
                                                         atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  1:1791522/1‑140 (MQ=255)
                                                         atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:3487359/1‑140 (MQ=255)
                                                           caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                              >  2:2378457/1‑140 (MQ=255)
                                                           caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                              >  2:228149/1‑140 (MQ=255)
                                                           caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAAGCGTCa                                                              >  1:1703601/1‑140 (MQ=255)
                                                                 tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                        >  2:659982/1‑140 (MQ=255)
                                                                   cGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTAGTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                      >  1:918607/1‑140 (MQ=255)
                                                                     tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                    <  1:3847801/140‑1 (MQ=255)
                                                                     tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCGTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                    <  1:3154060/140‑1 (MQ=255)
                                                                      aCAATACCATTAGATTTCATAAAATTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:1091197/140‑1 (MQ=255)
                                                                      aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:4115949/140‑1 (MQ=255)
                                                                        aaTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtttg                                                 >  2:2504877/1‑140 (MQ=255)
                                                                               gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTTTTTGCTattga                                          >  1:732383/1‑140 (MQ=255)
                                                                               gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                          <  1:1634776/140‑1 (MQ=255)
                                                                                  gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAGCAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgattg                                       >  1:2720302/1‑140 (MQ=255)
                                                                                   aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                      <  2:1540472/140‑1 (MQ=255)
                                                                                    tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  2:2917085/140‑1 (MQ=255)
                                                                                    tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:3487359/140‑1 (MQ=255)
                                                                                    tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:2504877/140‑1 (MQ=255)
                                                                                    tttCATAAACTGTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:1912544/140‑1 (MQ=255)
                                                                                    tttCATAAACGTTCTCCTCCCAAGAGATGATGATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACCAAATCCTTACCTTCCTAGCATAACAAGGTTTCCAATCATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:4661428/140‑1 (MQ=255)
                                                                                     ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                    >  1:251526/1‑140 (MQ=255)
                                                                                       cATAAACTTTCTCCTCCCAAGAGATGTGTATGTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATGCCAAGGTTTCCAATAGTCGTCAATCCGTGTGTTTGCTAGTGTTTGTCAGa                                  >  1:4934110/1‑140 (MQ=255)
                                                                                       cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgatt                                        <  2:1978320/134‑1 (MQ=255)
                                                                                       cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGa                                  >  1:2472591/1‑140 (MQ=255)
                                                                                       cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGa                                  >  1:2404248/1‑140 (MQ=255)
                                                                                          aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:2442260/140‑1 (MQ=255)
                                                                                          aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:460171/140‑1 (MQ=255)
                                                                                          aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  1:613685/140‑1 (MQ=255)
                                                                                          aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  1:2070603/140‑1 (MQ=255)
                                                                                          aaaCTATCTCCTCCCCAGACATGATTATTTGATTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCAGAGCATAACAAGGTTTCCAATAATCGTCAATCATGTTGTTTGCTATTGATTGTAAGAAtt                               <  2:4242225/140‑1 (MQ=255)
                                                                                            aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                             <  1:2011702/140‑1 (MQ=255)
                                                                                            aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                             <  1:3572228/140‑1 (MQ=255)
                                                                                                 ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                        <  2:2532009/140‑1 (MQ=255)
                                                                                                   cctccCAAGAGAGGATTATTTGTTTATATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGGATAACCAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                      <  2:4852398/140‑1 (MQ=255)
                                                                                                      ccccAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                   <  2:902516/140‑1 (MQ=255)
                                                                                                      cccAACAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                   <  1:3640444/140‑1 (MQ=255)
                                                                                                         aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCCGAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                <  1:3441038/140‑1 (MQ=255)
                                                                                                         aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                <  1:2979228/140‑1 (MQ=255)
                                                                                                           gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              <  1:1604141/140‑1 (MQ=255)
                                                                                                           gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              <  1:587669/140‑1 (MQ=255)
                                                                                                           gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              <  1:3603104/140‑1 (MQ=255)
                                                                                                             gatgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt            <  1:4834279/140‑1 (MQ=255)
                                                                                                              atgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           >  1:978130/1‑140 (MQ=255)
                                                                                                              atgatTATTTGTTTAAATTATATTTTTCTACGTCATTCTTCAACAAAATCCTTACCTTCATCGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           <  1:3105371/140‑1 (MQ=255)
                                                                                                              atgatTATTTGTTTAAATTATATTGTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           <  1:1220926/140‑1 (MQ=255)
                                                                                                                gatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTa         >  2:2985041/1‑140 (MQ=255)
                                                                                                                     tttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGa    >  2:3566625/1‑140 (MQ=255)
                                                                                                                      ttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaa   >  2:4757275/1‑140 (MQ=255)
                                                                                                                      ttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaa   >  2:1675541/1‑140 (MQ=255)
                                                                                                                      ttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaa   >  2:3878226/1‑140 (MQ=255)
                                                                                                                       tGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaaa  <  2:5075648/140‑1 (MQ=255)
                                                                                                                                   atTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              >  2:1614623/1‑116 (MQ=255)
                                                                                                                                       |                                                                                                                           
TTCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAA  >  NZ_CP034943/44730‑44988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: