Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 8.2% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA 8.2% 924.5 / 10.0 384Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (214/122);  new base A (2/46);  total (216/168)
Fisher's exact test for biased strand distribution p-value = 2.64e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

GAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316128‑316401
                                                                                                                                      |                                                                                                                                            
  aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCt                                                                                                                                      >  2:747750/1‑140 (MQ=255)
      tACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGcc                                                                                                                                  <  2:2955948/140‑1 (MQ=255)
      tACATAAGGCTTACGGTTAAAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGTCAAACCAATTAAGATAAAGGTCGAGACAGTATGTGACATTTTTGGGACCCCAACAAAGAAAGCAGTTCAAATGCTTGcc                                                                                                                                  >  2:277462/1‑140 (MQ=255)
           aaGTGTTACGGTTATAAGGATTGTTTAATAGGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACgg                                                                                                                             >  2:4788231/1‑140 (MQ=255)
             gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGATTGCCAACGGaa                                                                                                                           >  1:1627291/1‑140 (MQ=255)
               gtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAg                                                                                                                         >  1:4705312/1‑140 (MQ=255)
                ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAAGCAATTCAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGCAACAAAGACAGAAAGCCGGTCAAATGCTTGCCCACggggcgc                                                                                                                        >  2:1368609/1‑135 (MQ=255)
                  aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  2:4304530/1‑140 (MQ=255)
                  aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  1:2272153/1‑140 (MQ=255)
                  aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  1:196461/1‑140 (MQ=255)
                  aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCACTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  2:1678456/1‑140 (MQ=255)
                       tataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaa                                                                                                                 >  2:3943441/1‑140 (MQ=255)
                        ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAATTTTTTGGGAAACAAACAAAGAAAGCGGTTCAAATGCTTGCCAACGGAACGACaaaaaac                                                                                                                >  2:5190309/1‑139 (MQ=255)
                        ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:1087598/1‑140 (MQ=255)
                        ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACacaaaaa                                                                                                                >  2:1512323/1‑140 (MQ=255)
                        ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:2410901/1‑140 (MQ=255)
                        ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAAGTGTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:360455/1‑140 (MQ=255)
                           aGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAagag                                                                                                             <  1:1341740/140‑1 (MQ=255)
                              aTTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATc                                                                                                          >  2:924813/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGTAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:3479202/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:3069763/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:3614278/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:1255452/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:2053951/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:2124268/1‑140 (MQ=255)
                                tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTATGGGAAACAAACAAAGAAAGAAGTTCAAATGCTTGACAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:1006736/1‑140 (MQ=255)
                                  tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  1:818026/1‑140 (MQ=255)
                                  tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  1:1848006/1‑140 (MQ=255)
                                        atGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGa                                                                                                >  1:1209151/1‑140 (MQ=255)
                                            aGGGAAAATATGAGTGTAGATGAGAAACCAATTAATATAAAAGTCGAGAAAGGATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAAGGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:2338607/1‑140 (MQ=255)
                                            aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:362571/1‑140 (MQ=255)
                                            aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:3758448/1‑140 (MQ=255)
                                            aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:2833402/1‑140 (MQ=255)
                                            aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:5233881/1‑140 (MQ=255)
                                              ggAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACACAAAAAGAGATCCTGAAAGCGACCGGAt                                                                                          >  2:4173618/1‑140 (MQ=255)
                                              ggAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGAt                                                                                          >  2:4873653/1‑140 (MQ=255)
                                                     attaGTGTAGATGCCAAACCAATTAAGATAAAAGTCTAGAAAGTATCTAATATTTTTGGCAAACAAACAAAGAAAGCAGTCAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGt                                                                                   <  2:453337/137‑1 (MQ=255)
                                                     atGATTGTAGATCATAAACCAATTAATATAAAAGTCGAGAAAGTATATAAAATTTTTGGGAAACAAACAAAGAAAGCCGTTCAAATGCTTGCAACCGGAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGt                                                                                   <  2:4599949/140‑1 (MQ=255)
                                                         ttttAGATGAGAAACCAATTAAGATAAAAGTCGATAAATTATATAAAATTTTTGGCAAAAAAAAAAAGAAAGCTGTTAAAATGCTTGCCAACGCAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTggg                                                                               <  1:177673/137‑1 (MQ=255)
                                                         ttgtATATAACAAACAAATTAATAAAAACTTCGACAACTTATATAAATTTTTGGCCAAAAAAACAACCAAACCAGTAAAACGCGTTCCCACCCTAAACAAAAAAAAAGACACCCCGAAAGCGACCGGATCAACCGTTggg                                                                               <  1:484177/139‑1 (MQ=255)
                                                          tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:2558937/1‑140 (MQ=255)
                                                            tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                            >  1:330956/1‑140 (MQ=255)
                                                              gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaaa                                                                          >  1:1069383/1‑140 (MQ=255)
                                                                    cacccAATAACGACAAACGTCGCGAAAGGACCTAAAATTGTGGGCAAACAAAAAAACAACGCATTTCAATGCCTGGCCACCGGACCGCCAAAAAACCACATCGTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  1:907667/137‑1 (MQ=255)
                                                                    aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  2:2491996/140‑1 (MQ=255)
                                                                    aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:3218138/1‑140 (MQ=255)
                                                                    aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:199826/1‑140 (MQ=255)
                                                                      aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                  >  1:2474483/1‑140 (MQ=255)
                                                                      aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAg                                                                  >  1:680591/1‑140 (MQ=255)
                                                                      aCCAATTAAGATAAAAGTCGAGAAAGTAGCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                  >  2:780978/1‑140 (MQ=255)
                                                                         aaTTAATATAAAAGTCTATAAAGTATATAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTAAAAGGCTTGCCAACGGAAAGAAAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                               <  2:2125639/140‑1 (MQ=255)
                                                                           ttAAGATCAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtttt                                                             >  2:1638682/1‑140 (MQ=255)
                                                                            tAAGCTACAAGCCCAGAAACTAACTAAAATTTTGGCTAACCAAACAACGAACCCACTAAAAATGATTGCCAACCTCAATACAAAAAACCAGACCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:1744804/140‑1 (MQ=255)
                                                                            tAAGATAAAAGTCGAGAAAGTATCTAAAATTTTGGGCAAAAAAACAAACAAAGCAGTTCAAATGGTTCCCACCGCAAAGAAAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:4623854/140‑1 (MQ=255)
                                                                            tAAGAAAAAAGTAGACAATCTATCTAAAATTTTTGGTAAACAAAAAAATAAAGCAGTTCAAAGGCTTGCCAACGCAACTACAAAAAAAGTGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:4411645/140‑1 (MQ=255)
                                                                            tAACATAAAAGTCGACAACGTATCTAAAATTTTGGGCAAAAAAACAAACAAACCAGTTCAAAGCCTTGCCAACGCAAACAAAAAAAAACAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:943616/140‑1 (MQ=255)
                                                                            tAACATAAAAGTCGACAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:4710996/140‑1 (MQ=255)
                                                                            aaacaaaaaaCTCCACAAAGTATATAAATTTTTTGCCAACAAAACAAACAACCCCGTTCAAAGGCTTCCCAACGCAAACACAAAAAAAGACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:3943441/134‑1 (MQ=255)
                                                                              ataTAAAAGTCTATAAAGTATATAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:1227086/138‑1 (MQ=255)
                                                                              acaaaaaaGTCGACAAAGTATATAAAATTTTTGGTAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:3504237/136‑1 (MQ=255)
                                                                              aGATAAAAGTCGACAAATTAAAAAAATTTTTTGGGAAACAAACAAACAACCCAGTAAAAATGCTGCCCACCGCAAACACAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:3625574/140‑1 (MQ=255)
                                                                              aGACAAAAGCCGACACAGTAACTAAAATTTTTGGCAAACAAACAAACAACCCAGTTCAAATGCTTCCCACCGCAAACACAAAAAAAGAGACCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:4382482/140‑1 (MQ=255)
                                                                              aGAAAAAAGTCGAGAAAGTAACTAAAATTTTTGGGAAAAAAAAAAAGAAAGCAGTTAAAATGCTTCCCACCGCAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:3836771/140‑1 (MQ=255)
                                                                                ctAAAAGTCGCGAAAGTATCTAAACTTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGt                                                        <  2:5044279/139‑1 (MQ=255)
                                                                                   aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                     <  2:1049290/140‑1 (MQ=255)
                                                                                    aaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACACAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTatat                                                    >  2:3485120/1‑140 (MQ=255)
                                                                                    aaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTatat                                                    >  2:4089408/1‑140 (MQ=255)
                                                                                    aaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATGTTGATGTatat                                                    >  2:2900378/1‑140 (MQ=255)
                                                                                      ctctataaaGTTTATAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatga                                                  <  2:3868820/134‑1 (MQ=255)
                                                                                        ctataaaGTATATAAAATTTTTGGTAAACAAAAAAAGAAAGCAGTTCAATTGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatgatg                                                <  2:4467334/136‑1 (MQ=255)
                                                                                         gataaaTTATATAAATTTTTTGTTAAAAAAAAAATTAAAGCTTTTCAAATGCTTGCAATCGTAAACAAAAAAAAACATTTGCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               <  1:1678456/137‑1 (MQ=255)
                                                                                         gagaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               >  1:4536927/1‑140 (MQ=255)
                                                                                         gagaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               >  1:511798/1‑140 (MQ=255)
                                                                                         gagaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATCTATATGATgg                                               >  2:3420493/1‑140 (MQ=255)
                                                                                         cacaaaGTATCTAAATTTTTTGGCAAACAAACAAACAACCCATTTAAATTGCTTGCCAACGGAAAGACAAAAAAATACACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               <  1:4304530/137‑1 (MQ=255)
                                                                                              aGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgaga                                          <  2:4131873/140‑1 (MQ=255)
                                                                                                 aTCTAAATTTTTTGGTAAACAAAAAAAGAAAGCAGTACAAAGGCTTGCCACCGTAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       <  2:4986096/140‑1 (MQ=255)
                                                                                                        aTTTTTGGGAAACAAACCACGAACGCAGTTCAACTGCTGCCCAACGCAAACAAAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcat                                <  1:1006736/140‑1 (MQ=255)
                                                                                                         tgtttGGCAAACAAACAAAGAACCCACTTCACATGGTTGCCACCGCAAAGAAAAAAAAAGATATCCTTAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                               <  1:3374137/138‑1 (MQ=255)
                                                                                                           tttcgcaaacaaacaaacAAAGCAGTTCAATGGCTTCCCAACGGAAAGACAAAAAAAGACAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:3054733/134‑1 (MQ=255)
                                                                                                           tttGGTAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCAAACGCAAACACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:2035307/140‑1 (MQ=255)
                                                                                                           tttGGGAAACAAACAAAGAAAGCGGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:2413370/140‑1 (MQ=255)
                                                                                                           tttGGCAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:330956/140‑1 (MQ=255)
                                                                                                           tttGGCAAACAAACAAAGAAACCATTTAAAATGCTTGCCAACGCAAACACAAAAAAAGACAGCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:4993889/140‑1 (MQ=255)
                                                                                                             tGGGAAACAAACAAATAAAGCATTTCAAATGCTTGCCACCGGAAAGACAAAAAATGATATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:2272153/140‑1 (MQ=255)
                                                                                                             tGGGAAAAAAACAAAGAACGCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:270164/140‑1 (MQ=255)
                                                                                                             tGGCAAACAAAAAAATAAAGCAGTACAAATGCTTGCCAACGCAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:577586/140‑1 (MQ=255)
                                                                                                             tGGCAAACAAAAAAAGAAAGCAGTTCAAATGCTTCCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:1925454/140‑1 (MQ=255)
                                                                                                             ggggAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGTAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:2252114/139‑1 (MQ=255)
                                                                                                             gggcaaacaaaaAAAGAAACCATTACAATGGCTGGCCAACCCAAACAAAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:4137112/136‑1 (MQ=255)
                                                                                                                gAAACAAACAAAGAAAGCCGTTCAAATGCTTGCCAACGCAAAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTc                        <  2:3148644/140‑1 (MQ=255)
                                                                                                                  aacaaaaaAATAAACCCTTTCAATTGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTa                      <  2:5078103/140‑1 (MQ=255)
                                                                                                                       acaaacaaAGCAGTTCAAATGCTTGCCAACGTAAAGACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAgg                 <  2:2672650/140‑1 (MQ=255)
                                                                                                                       acaaaGAACGCTGTACAAATGCTTCCAACCGCAAACAAAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAgg                 <  1:775067/140‑1 (MQ=255)
                                                                                                                        aaaacaaagCATTTAAAATCGTTGCCAACTGAAAGACAAAAAAAGAGATCGTTAAAGCGACCTGTTCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg                <  2:3699449/139‑1 (MQ=255)
                                                                                                                               agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa         >  2:3223657/1‑140 (MQ=255)
                                                                                                                                  aGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  2:3069688/140‑1 (MQ=255)
                                                                                                                                   tttCAAATGCTTGCCACCGGAAACACAAAAAAAGAGATCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  2:1755726/139‑1 (MQ=255)
                                                                                                                                    ttCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCa    >  2:4905896/1‑140 (MQ=255)
                                                                                                                                      cAACTGCTTGCCAACGTAAAGAAAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc  <  2:875185/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                            
GAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316128‑316401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: