Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 399,035 C→A 8.2% V949V (GTC→GTA srfAC → surfactin non‑ribosomal peptide synthetase SrfAC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943399,0350CA 8.2% 570.5 / 9.7 246V949V (GTC→GTAsrfACsurfactin non‑ribosomal peptide synthetase SrfAC
Reads supporting (aligned to +/- strand):  ref base C (135/82);  new base A (3/26);  total (138/108)
Fisher's exact test for biased strand distribution p-value = 8.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CAACGCATACCTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTCATGATGA  >  NZ_CP034943/398897‑399172
                                                                                                                                          |                                                                                                                                         
  aCGCATACCTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAAt                                                                                                                                        >  1:424073/1‑140 (MQ=255)
   cGCATACCTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATa                                                                                                                                       >  1:465968/1‑140 (MQ=255)
        aCCTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGa                                                                                                                                  >  1:834746/1‑140 (MQ=255)
        aCCTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGa                                                                                                                                  >  1:1793671/1‑140 (MQ=255)
          cacGTTAACCGGAAGCAGCTTTCAGCTAAAGACGTAAAGGCGCACTTTAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTCTCTTGGACGAGGTTCCTTTAACCACAAACGGGAAGGTCAATAAACGAct                                                                                                                                <  2:3073832/138‑1 (MQ=255)
          cTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGGGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGAct                                                                                                                                <  2:2633934/140‑1 (MQ=255)
           tCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGActg                                                                                                                               >  1:226397/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTCAGCTCAATACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCAACAAACCTTTACTTTCTTGGACGAGCTTCCATTAACGACAAACGGGACGGTCAATAAACGAcagct                                                                                                                             >  2:412789/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGACGGTCAATAAACGActgct                                                                                                                             >  2:4265434/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGActgct                                                                                                                             >  2:2910482/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGActgct                                                                                                                             >  2:2007632/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGACACAATCCTTTAATTTCTTGGACGAGCTTCCTGTAACGACAAACGGGCAGGGCAATGAACGActgct                                                                                                                             >  2:2995891/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACGTGAAAAAACAGCTTCCTGATTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGATTCCTTTAAAGACAAACGGGAAGGTCAATAAAAGAcggct                                                                                                                             >  1:472607/1‑140 (MQ=255)
             gTGAACCGGACGCAGCTTTAAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTAACAATTTGCCACAAACCTTTACTTTCTTGGACTAGCTTCATTTGACTAAAAACGGGAACGTCAATAAAAGaaagct                                                                                                                             >  2:3125871/1‑135 (MQ=255)
              tGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGActgctg                                                                                                                            >  1:4925372/1‑140 (MQ=255)
              tGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGActgctg                                                                                                                            >  1:2581065/1‑140 (MQ=255)
              tGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTAAATGGTGCCACAAACCTTTACTTTCTTGGACGAGCGTCCTTTAACGACAAACGGGAAGGACCATAAACGActgctg                                                                                                                            >  2:1531275/1‑140 (MQ=255)
              tGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACATCAAAAAACAGCTTCCTGCTTACAAGGTGCCACACACCGTTAATTTCTTGGACGCGCTTCCTTTAACCACAAACCGCAAGGTCACTAAACGActgatg                                                                                                                            >  2:2928696/1‑140 (MQ=255)
                  ccGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCaa                                                                                                                        >  2:3308039/1‑140 (MQ=255)
                    ggACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCaaaa                                                                                                                      >  2:1097743/1‑140 (MQ=255)
                     gccGCAGCTTTCAGCTCAAGACGTAAAGGCGAACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAACCGGGAAGGTCAATAAACGACTGCTGCCAAAAc                                                                                                                     <  2:2439248/138‑1 (MQ=255)
                     gACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAAc                                                                                                                     >  1:972840/1‑140 (MQ=255)
                     gACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAAc                                                                                                                     <  2:1414007/140‑1 (MQ=255)
                       cGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCg                                                                                                                   >  2:763947/1‑140 (MQ=255)
                          aGCTTTCAGCTCAAGACTTAAAGGCGCACTTTAAAAAACAGCTTCCTGCTTACTTGGTGCCACAAACATTTACTTTCTTGGACGAGCTTCATTTAACGACAACCGGGAAGTTAAATAAACGACTGCTGCCAAAACCggat                                                                                                                <  1:1811508/140‑1 (MQ=255)
                          aGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCggat                                                                                                                >  1:947931/1‑140 (MQ=255)
                          aGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCggat                                                                                                                <  2:323187/140‑1 (MQ=255)
                          aGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCggat                                                                                                                <  2:2102772/140‑1 (MQ=255)
                                  gCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCggatcaggatca                                                                                                        >  2:5164282/1‑140 (MQ=255)
                                      aaGACGTAAAGGCGCACTTGAAAAAACAGCTTCGTGCTTACAGGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGCAGGTAACTCAACGACTGCTGCCAAAACCGGATCAGGATctgctg                                                                                                    >  1:3249576/1‑140 (MQ=255)
                                      aaGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTg                                                                                                    >  1:2914503/1‑140 (MQ=255)
                                       tggcGTCAATGCGCACTTGACAACACGGTTTCCTGGTTACGTGGTGCAACAACCCTTTAGTTTGTTGGACGAGCTTCATTTAAAGACACACGGCATGGTAAATAACCGACTGCTGCCAAAACCGGATCAGTATCAGCTgg                                                                                                   <  2:3357075/137‑1 (MQ=255)
                                       aGACGTAAAGGCGCACTGTAAAAAACAGCTTCCTGATTACATGGTGCCACAAACCTTTACTTTATTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGCATCAGGATCAGCTgg                                                                                                   <  2:3920229/140‑1 (MQ=255)
                                         acttAACGGCGAACTCGAAAAAACAGCTTCCTGCTTCCTTGGTGCCAAAATCGTTTACTTTCTTGGACTAGCTTCCTTTAACCACAACGGGGATGGTCAATACACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCg                                                                                                 <  1:1998270/137‑1 (MQ=255)
                                         aCGTACAGGCGCACTTTAAAAAACTGCTTCCGGCTTACCTGGTGCCACAAACCTTTACTTTCTTGGACGAGGTTCCTTTAACGACACCCGGGACGGTAAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCg                                                                                                 <  1:2239089/140‑1 (MQ=255)
                                         aCGTAATGCCGCAGTTGAACAAACAGATCCCTGCTTACATTGTCCCACAAACATTTAGTTTGTTGGACGAGGTGCCTTTCTGTACAAAGGGGAAGGTCAATAAACGACTGCTGCCAACCCCGGATCAGGATCAGCTGGCg                                                                                                 <  1:2354796/140‑1 (MQ=255)
                                         aCGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGGTTCCTTTAACGACAAACGGGAAGTTAAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCg                                                                                                 <  1:219543/140‑1 (MQ=255)
                                         aCGAAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAACCCTTTACTTTCTTGGACGCGCTTCGTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCg                                                                                                 <  1:4000960/140‑1 (MQ=255)
                                         aCGAAAAGGCGCAATTCAAAAAACAGCTTCCTGCTTACATGGTCCCACAAACCTTTCCTTTCTTGCCCGAGCTTCCTTTACCGACAACCGGGAAGGTAAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCg                                                                                                 <  1:2028991/140‑1 (MQ=255)
                                            tAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGgaa                                                                                              >  1:3150751/1‑140 (MQ=255)
                                              aaGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGgaaga                                                                                            <  1:5027022/140‑1 (MQ=255)
                                                  cgcACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATgg                                                                                        >  1:3119445/1‑140 (MQ=255)
                                                  cgcACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATgg                                                                                        >  1:4194679/1‑140 (MQ=255)
                                                    cACTTTAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTAGTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTAAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGAt                                                                                      <  1:2490775/140‑1 (MQ=255)
                                                    cACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAACCGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGAt                                                                                      <  2:3500060/140‑1 (MQ=255)
                                                    cACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGGCAAAACCGGATCAGGATCAGGTGGCGGACGAATGGAt                                                                                      >  2:1840427/1‑140 (MQ=255)
                                                    cACTTGAAAAAACAGCTTCCTGATTACATGGTGCGACAAACCTTTACTTTCTTGGACGAGGTTCCTGTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACAGGATCTGGATCAGCGGGCGTAAGAATGTAt                                                                                      >  2:1215179/1‑140 (MQ=255)
                                                      cTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCg                                                                                    <  2:834746/140‑1 (MQ=255)
                                                           aaaaaCAGGTTCCTGCTGACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAACCGGGAAGGTAAATACACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCa                                                                               <  1:3416027/140‑1 (MQ=255)
                                                                ccgCTTCCTGATTACATGGTCCCACAACCCTTTACTTTCTTGGACGCGCTTCCTTTAACGACAAACGTGAACCTCAATACACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCacgaga                                                                          <  2:4103019/138‑1 (MQ=255)
                                                                cAGCGTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACTAGCTTCCTTTAACGACAAACGGGAAGGTAAATACACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCacgaga                                                                          <  1:1299925/140‑1 (MQ=255)
                                                                 aGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCacgagac                                                                         >  1:1577376/1‑140 (MQ=255)
                                                                     tCCTGCTTACATGGTGCCACAACCCTTTAGTTTCTGGCACGAGGTACCTTTAACGAAAAACGGGAAGGTCAATACCCGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCAcgagacgaga                                                                     <  1:4987097/140‑1 (MQ=255)
                                                                     tCCTGCTTACATGGTGCCACAAACGTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCAcgagacgaga                                                                     <  1:3465645/140‑1 (MQ=255)
                                                                     tCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACCACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCAcgagacgaga                                                                     <  1:3497827/140‑1 (MQ=255)
                                                                         gCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGga                                                                 >  1:1132557/1‑140 (MQ=255)
                                                                          cTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGgaa                                                                <  2:4369611/140‑1 (MQ=255)
                                                                                  gTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGAt                                                        <  1:4500766/140‑1 (MQ=255)
                                                                                    gCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACTACTGCTGCCAAAACAGGATCAGGATCAGCTGGCGTAAGAATGGATCGGGCCACGAGACTAGAGGGAAGAAACGATCg                                                      >  1:4863590/1‑140 (MQ=255)
                                                                                    gCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGACACGATCg                                                      >  1:5194662/1‑140 (MQ=255)
                                                                                    gCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCg                                                      >  1:1039242/1‑140 (MQ=255)
                                                                                    gCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCg                                                      >  1:3649450/1‑140 (MQ=255)
                                                                                    gCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCg                                                      >  1:4341832/1‑140 (MQ=255)
                                                                                    gCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCg                                                      >  1:786430/1‑140 (MQ=255)
                                                                                     ccACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGACGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGACACGATCGc                                                     >  1:2983986/1‑140 (MQ=255)
                                                                                     ccACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGc                                                     >  1:1646896/1‑140 (MQ=255)
                                                                                      cacaAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGca                                                    <  1:3974042/140‑1 (MQ=255)
                                                                                       acaAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGcac                                                   >  2:3736285/1‑140 (MQ=255)
                                                                                             cTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAAttt                                             >  2:4192932/1‑140 (MQ=255)
                                                                                               ttACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTgg                                           >  1:628338/1‑140 (MQ=255)
                                                                                                tACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGt                                          >  2:5006499/1‑140 (MQ=255)
                                                                                                tACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGAGCGGGCCACGAGACGAGACGGAAGAACCGATCGCACAAATTTGGt                                          >  1:3300328/1‑140 (MQ=255)
                                                                                                   tttCTTGGACGTGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCgg                                       <  2:2958235/140‑1 (MQ=255)
                                                                                                   tttCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCgg                                       <  1:5164282/140‑1 (MQ=255)
                                                                                                   tttCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCgg                                       <  2:5216089/140‑1 (MQ=255)
                                                                                                     tCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAg                                     >  2:4596021/1‑140 (MQ=255)
                                                                                                     tCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAg                                     >  2:2153152/1‑140 (MQ=255)
                                                                                                     tCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTGGGTCGGAg                                     <  2:1577376/140‑1 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTATCGACATACGGGACGGTCCATAAACGAGTGGTGCGAAAGGCGGATCCGGTTCAGGGGGCGGAAGAAGGGATCGGGCCAGGAGACGAGACGGACGAACGGAGCGCGCAAATGTGGGCGGCGGt                                   >  2:4766688/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:456847/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:2380391/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:601989/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:3703799/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:712119/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:1370560/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:2556423/1‑140 (MQ=255)
                                                                                                       ttGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGt                                   >  2:2411012/1‑140 (MQ=255)
                                                                                                             gAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAACACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCgg                             <  1:3573765/140‑1 (MQ=255)
                                                                                                                         aCGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGt                 >  2:4043452/1‑140 (MQ=255)
                                                                                                                             cAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAACCGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGc             >  2:4930015/1‑140 (MQ=255)
                                                                                                                             cAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGc             >  2:4398358/1‑140 (MQ=255)
                                                                                                                              caaCGGGAAGGTCAATAACCGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCa            <  2:2300569/139‑1 (MQ=255)
                                                                                                                              aaaCGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCCCAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCa            >  2:1657339/1‑140 (MQ=255)
                                                                                                                              aaaCGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCa            <  1:149256/140‑1 (MQ=255)
                                                                                                                                 ccggAAGCTCAATAAACGACTGCTGCCATAACCGGATCAGGATCTGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATGTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTc         <  2:863555/138‑1 (MQ=255)
                                                                                                                                 cGGGAAGGTCAATACACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTc         <  2:740123/140‑1 (MQ=255)
                                                                                                                                     ccggTCAATCAACGACTGCTGCCCACACCGGATCAGGCTCAGCTGGCGGAAGAATGGATCGGGCCCCGAGACGACACGGAAGAAACGATCGCACAACTTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTCatga     <  2:3448861/138‑1 (MQ=255)
                                                                                                                                        gTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTCAtgatga  <  1:413457/140‑1 (MQ=255)
                                                                                                                                        gTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTCAtgatga  <  1:2719639/140‑1 (MQ=255)
                                                                                                                                        gTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTCAtgatga  <  1:1097743/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CAACGCATACCTCGTGAACCGGACGCAGCTTTCAGCTCAAGACGTAAAGGCGCACTTGAAAAAACAGCTTCCTGCTTACATGGTGCCACAAACCTTTACTTTCTTGGACGAGCTTCCTTTAACGACAAACGGGAAGGTCAATAAACGACTGCTGCCAAAACCGGATCAGGATCAGCTGGCGGAAGAATGGATCGGGCCACGAGACGAGACGGAAGAAACGATCGCACAAATTTGGTCGGAGGTTCTCGGCAGACAGGAAGTTGGCATTCATGATGA  >  NZ_CP034943/398897‑399172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: