Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,359 A→C 13.5% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,3590AC13.5% 380.3 / 10.5 192T68P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (54/99);  new base C (4/31);  total (59/133)
Fisher's exact test for biased strand distribution p-value = 4.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498
                                                                                                                                          |                                                                                                                                           
gCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGACCGAc                                                                                                                                            >  1:679730/1‑140 (MQ=255)
 ccACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           >  2:3836831/1‑140 (MQ=255)
   ccccgAGCCACAGGGGAACCTGGCCCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                                                         <  2:2853183/136‑1 (MQ=18)
    cGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGa                                                                                                                                        <  2:4231269/140‑1 (MQ=255)
    cGGGAGCTACGGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGTAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGa                                                                                                                                        <  1:2198083/140‑1 (MQ=255)
     gggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAg                                                                                                                                       <  1:4698232/140‑1 (MQ=255)
     gggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAg                                                                                                                                       <  1:4464738/140‑1 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  >  1:4919067/1‑140 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:1395459/140‑1 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGAACAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:638042/140‑1 (MQ=255)
            acgggggAAGCTGGCCCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  2:5166414/137‑1 (MQ=255)
            aCTGGGGAAGCTGGCCCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:1034936/140‑1 (MQ=255)
            aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  2:4798716/140‑1 (MQ=255)
            aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:3993224/140‑1 (MQ=255)
            aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  2:4120791/140‑1 (MQ=255)
            aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  2:1004041/140‑1 (MQ=255)
                gggAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGcc                                                                                                                            <  2:3061625/140‑1 (MQ=255)
                gggAAGCTGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGTGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGcc                                                                                                                            <  2:1755476/140‑1 (MQ=255)
                  gcaGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGCGCTACCGGGGACGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCAc                                                                                                                          <  2:2034798/138‑1 (MQ=255)
                     gCTGGACCAGGCGGAGCAGCTGGCGCCCCGGTACGCACGGGAGCTCCGGGTGAGGCTGGCCGTGCCGGGGCGGCGGGCGCGACAGGAGCGAGCGGGGCACCGGGACCAGCGGGACCAACCGGAGCGACGGGAGCCACcgg                                                                                                                       <  2:3276357/140‑1 (MQ=12)
                     gCTGGACCAGCCGGACCTGCGGGAGCCACGGGAGCCACTGGAGCTACGGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:2683806/140‑1 (MQ=18)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  1:4869788/1‑140 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:3671004/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:2316125/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCCGCCGGAGCGACGGGCGCGACCGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACcgg                                                                                                                       >  2:3598421/1‑140 (MQ=21)
                     gCTGGACCAGCCGGACCAGCCGGAGCCACGGGACCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:2559766/140‑1 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAg                                                                                                                     <  2:2755306/140‑1 (MQ=255)
                       gggccccgccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGCAGCTGGACCAGCCGGAGCGCCGGGAGCGAGCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAg                                                                                                                     <  2:4455473/133‑1 (MQ=21)
                          cccagccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:2176266/139‑1 (MQ=255)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  >  1:1692896/1‑140 (MQ=255)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGCCGGGAGCGACGGGAGCCACGGGAGcga                                                                                                                  >  1:665091/1‑138 (MQ=18)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  1:840179/140‑1 (MQ=255)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGACCGGAGCGACAGGAGCAACCGGACCAGCCGGACCAACCGGCGCGACGGGAGCGACGGGGGcga                                                                                                                  >  2:3499042/1‑138 (MQ=11)
                              gccggaccagcgggaGCGACGGGAGCCTGGGGAGCTCGCGGGTAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              <  2:2647141/140‑1 (MQ=21)
                              ccccgacccgccggaCCCACGGGAGCCACGGGAGCTACCGGGGACCCTGGACCAGCCGGAGCGACGGGCGCCACCGGAGCTACCTGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              <  2:4060955/136‑1 (MQ=17)
                               ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  2:868977/140‑1 (MQ=255)
                                   accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACCGGAGCGACCGGAGCCACGGGAGCTACCGGGGAag                                                                                                         >  1:4023505/1‑140 (MQ=18)
                                   accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  1:4829783/140‑1 (MQ=255)
                                   accagccggaGCGACGGGAGCCACCGGAGCTACCGGGGAATCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACAGGGGCAGCCGGACCCGCCGCACCACCCGGAGCGACGCGAGCCACGGGAGCGCCCggggcgg                                                                                                         >  1:890381/1‑137 (MQ=18)
                                   accagccggaGCGACCGGAGCCACGGGAGCTACCGGCGAAGCTGGACCAGCCGGAGCGAGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  2:4287718/140‑1 (MQ=255)
                                        ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGCCGGGCGCGACGGGCGCGGCCGGAGCagccgga                                                                                                    >  1:1816608/1‑140 (MQ=17)
                                                aCGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAcgccggaccagccgg                                                                                            >  2:182844/1‑140 (MQ=255)
                                                      gCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              >  1:716750/1‑116 (MQ=255)
                                                      gCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  1:348957/140‑1 (MQ=255)
                                                       ccACTGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                     <  2:1883831/140‑1 (MQ=255)
                                                                cTACGGGTGATGCGGGACGAGCCGGAGGGACGGGAGCCACGGGAGCGACTGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  2:2373409/140‑1 (MQ=14)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACGGGGGAAGGCGGAGCACCCGGACCCACCGCAGCCACAGGAGCAACGGGAGATCCCGGTGATGCCGGACCACCAGGACCAACCggagcggcg                                                                            >  2:1143684/1‑137 (MQ=17)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:250937/140‑1 (MQ=255)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:3577877/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:4919067/140‑1 (MQ=255)
                                                                          aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                  <  1:3126171/140‑1 (MQ=255)
                                                                              ggACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGCAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                              <  2:3699597/140‑1 (MQ=255)
                                                                               gACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCt                                                             <  1:2717967/140‑1 (MQ=255)
                                                                                cccACCCGGAGCGACGGGAGCCACAGGAGCGACCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  1:1010084/139‑1 (MQ=14)
                                                                                     ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              >  1:1175129/1‑85 (MQ=255)
                                                                                     ccGGACCGCCGGGAGCCACAGGAGCTACCGGTGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  2:2259051/140‑1 (MQ=14)
                                                                                     ccGGACCGACGGGAGCGCCCGGCGCTACCGGCGATGCGGGACCCGCCCGCCCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  2:4768736/140‑1 (MQ=14)
                                                                                               gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             >  1:4797687/1‑140 (MQ=255)
                                                                                               gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             >  1:2899260/1‑140 (MQ=255)
                                                                                               gggAGCCACAGGAGCTACCGGCGCAGCCGGACCACCAGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:1935227/140‑1 (MQ=14)
                                                                                               cggagcaacaggagcaacaGGACAACCCGGACCAGCAGGACGAACCGGAGCGATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:1441004/139‑1 (MQ=14)
                                                                                                   gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCAACCGGCGAAGCCCGAGCACCCGGACCAACCGGAGCCACAGGAGCCACCGGAGCGACCGGGGCAGCCGGagcgcc                                         >  2:3846425/1‑135 (MQ=17)
                                                                                                    cGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  1:5021311/140‑1 (MQ=255)
                                                                                                        cggAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                    <  2:4649397/139‑1 (MQ=255)
                                                                                                          gAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAGCCggaccc                                  >  2:1688120/1‑137 (MQ=255)
                                                                                                             cTACCGGGGAAGCCGGACAAGCCGGCCCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                               <  2:1719349/140‑1 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             <  2:153824/140‑1 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGCCGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGAGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             <  1:1150177/140‑1 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              <  1:3374631/59‑1 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              >  2:3374631/1‑59 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             >  2:723555/1‑140 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGGGCAGCCGGAGCAGCCGGAGCTACCGGAGCGACCGGAGCGACAGGAGCTACCGGCGACCCCGGACCAGCCggagcgacagg                             >  2:4897868/1‑140 (MQ=14)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACCGGGGACTCCGGACCATCAGGGCCAACCCGAGACACCGTAGCATCTGGAGCTACCGGGGCAGCCGGACCCGCCggggcgccggg                             >  1:2157061/1‑135 (MQ=9)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:1985074/116‑1 (MQ=255)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:978202/116‑1 (MQ=255)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggag                           >  1:561845/1‑140 (MQ=255)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACAGGCGAAGCCGGAGCAGCGGGACCTAGCGGCCTGACCGGAGCCACAGGAGCTCCGGCGGAAGCGGGACCCGCGggcgcgagaggcg                           >  1:4992919/1‑138 (MQ=9)
                                                                                                                    ggAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:2512254/140‑1 (MQ=255)
                                                                                                                    ggAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:4869788/140‑1 (MQ=255)
                                                                                                                     gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCTCGCGGGGAAGCCGGACGCGCCgcaccgcccggagcgac                       >  1:4147726/1‑140 (MQ=17)
                                                                                                                     gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACCGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacgggagcggc                       >  1:234646/1‑138 (MQ=255)
                                                                                                                      cagccggaccagccggaccACCCGGAGCCCCGGGAGCCACGGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  1:2851194/139‑1 (MQ=255)
                                                                                                                      aaGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCGACCGGTGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  2:4509790/140‑1 (MQ=255)
                                                                                                                      aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  1:1342789/140‑1 (MQ=255)
                                                                                                                        gccggccccgccggaccaCCCCGAGCCACCGGAGCCCCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:4623057/140‑1 (MQ=17)
                                                                                                                        gccggaccagccggaccaCCCGGAGCGCCCGGAGCCACGGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:1218247/140‑1 (MQ=17)
                                                                                                                        cccggaccagccggaccaTCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:5035084/139‑1 (MQ=255)
                                                                                                                          cggagccgctggacctACCGGAGCCCCGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacgggagccacagggg                  <  1:1726424/140‑3 (MQ=14)
                                                                                                                          cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCCCCCggcgcgacaggagcgacaggag                  >  1:5108899/1‑140 (MQ=17)
                                                                                                                           ggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACCGGAGCGACAGGAGCGACAGGAGCCCGCGGCGCAGCCGGACCACCCggaccgagcggaccgacacgcgc                 >  2:294297/1‑135 (MQ=14)
                                                                                                                           ggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCTACCGGGGCAGCCGGACGGGCCgcggcggcgggcgcgacaggggg                 >  2:3136294/1‑137 (MQ=14)
                                                                                                                             cccagccggaccaCCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:3843877/139‑1 (MQ=255)
                                                                                                                             accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:4607929/140‑1 (MQ=255)
                                                                                                                             accagccggaccaACCCGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:832809/140‑1 (MQ=255)
                                                                                                                             accagccggacaaACCGGAGCGAGGGGCGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:3298067/140‑1 (MQ=17)
                                                                                                                             accacccggaccacccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:223106/140‑1 (MQ=255)
                                                                                                                                 gccggaccaCCCGGTGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  1:3816842/140‑1 (MQ=255)
                                                                                                                                 gccggaccaCCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  1:1147129/140‑1 (MQ=255)
                                                                                                                                 gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGCACCACCCGGAGCCACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  1:2025812/140‑1 (MQ=17)
                                                                                                                                  ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga          <  2:2899260/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: