BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,562	G→A	6.3%	G135G (GGG→GGA)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,562	0	G	A	6.3%	295.7 / 3.9	143	G135G (GGG→GGA)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (67/55); new base A (6/2); total (80/63)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

AGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGG  >  NZ_CP034943/607424‑607697

aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGATACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCgggg                                                                                                                                        >  1:143021/1‑140 (MQ=255)

 ggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGc                                                                                                                                       >  2:3383760/1‑140 (MQ=255)

    gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCCACCGGAGCAACCGGGGCAGCTGGACCAGCGGGAGCCACCGGAGCGACAGGAGCTACCGGGGAACCCGGAGCAGCCGGGGCCAc                                                                                                                                    >  2:4809708/1‑140 (MQ=17)

    gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACCGGAGCAACCGGGGACGCTGGACCAGCGGGAGCGCCCGGACCGACCGGAGCTCCCGGGGACGCCGGACCGGCCGGGGcccc                                                                                                                                    >  2:29943/1‑138 (MQ=18)

    gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                                                                    >  2:433196/1‑140 (MQ=255)

    gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCAc                                                                                                                                    >  2:1990038/1‑140 (MQ=255)

       ccaggagcTACCGGGGAAGCCGGACCAGCCGGAGCTAAAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:2061234/139‑1 (MQ=255)

       ccaggagcTACCGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:2060874/139‑1 (MQ=255)

       acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:4785591/140‑1 (MQ=255)

       acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 >  2:1119664/1‑140 (MQ=255)

       acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:3132458/140‑1 (MQ=255)

       acaggagcTACCGGGGAAGCCGCACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:4600406/140‑1 (MQ=255)

       acagcagcACCCGGCGAAGCCCGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCTGGGAAGCTTGACCAGCTGGAGCTACGGTGGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:4147513/140‑1 (MQ=18)

         aggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                                                               >  1:3801428/1‑140 (MQ=255)

             gcTCCCGGGGAAGCCGCACCAGCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGATACCTGGGAAGCTGGACCAGTTGTAGCTACTGTAGAGACATGAGCTACCGGGTTAGCAGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                           <  1:1652818/140‑1 (MQ=255)

              cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  1:433196/140‑1 (MQ=255)

              cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  1:430940/140‑1 (MQ=255)

              cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  1:2286899/140‑1 (MQ=255)

                cccGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                        <  1:2107229/139‑1 (MQ=255)

                aCCGGGGACGCCGGACCCGGCGGAGGCACCGGTGCCCCAGGCGCGTCCGGAGCAACCGGGCCCCCTGGCCCACCGGGAGCCACGGGAGCCACAGGACCAACCGGGGAAGCCGGACCCGCGGGGGCCACGGGAGCCACAgg                                                                                                                        <  2:2157211/140‑1 (MQ=255)

                aCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCAACCGGGGCAGCTGGACCAGCAGGAGCGACGGGAGCGACAGGAGCTACCGGAGCCGCCGGACCGGCGGGAGCGACCGGAGCAACAgc                                                                                                                        >  2:2387201/1‑139 (MQ=14)

                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGATCGACAGGAGCTACCGGAGAAGCTGGACCAGCTGGAGCGACTGGAGCGACAGGAGCTGCCGGTGCAGCCGGACCAGCCGGGGCGACGGGAGCCGCAgg                                                                                                                        >  1:786610/1‑140 (MQ=18)

                  cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                      >  2:2852027/1‑140 (MQ=255)

                  cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                      >  2:3765434/1‑140 (MQ=255)

                     ggcaGCCGGACCAGCCGGAGCACCAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                   <  1:4626058/137‑1 (MQ=18)

                     ggAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                   <  1:1688120/140‑1 (MQ=255)

                      gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGATACCGGGCAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGCAGCTACCGCGGAAGCCGGACCACCCCGGGCCACGGGAGCCACCGCGGCGAc                                                                                                                  >  2:4177203/1‑140 (MQ=18)

                         gCCGGACCAGCCGGAGCGACTGGAGCGACAGGAGCCACAGGAGCAACCGGGGAAGCTGGACCACCGGGAGCGACGCGAGCGACACGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACGGGGGCTACggg                                                                                                               >  1:5207337/1‑140 (MQ=17)

                              aCCCGCCGGCGCGACAGGAGCGACAGGCGCGCCCGGAGCCCGCGGCGCAGCTGGCCCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                          <  1:3846425/140‑1 (MQ=21)

                              aCCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGCGCTCCGGGGGAAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                          <  2:4023505/140‑1 (MQ=21)

                              aCCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCTCCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAg                                                                                                          >  2:2884030/1‑140 (MQ=255)

                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGCCGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGacg                                                                                                          >  2:4126772/1‑138 (MQ=255)

                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCCACAgggccag                                                                                                          >  2:4320185/1‑136 (MQ=17)

                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCGACAGGGGCTCAGGGGGAAGCCGGa                                                                                                     >  2:1587934/1‑140 (MQ=21)

                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                     >  1:4070879/1‑140 (MQ=255)

                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCAACCGGAGCCACCGGACCAGCCGGAGCGCGGGGAGCCCCAGGCGCGTCTGGAGCAGCCGGa                                                                                                     >  2:1587685/1‑140 (MQ=17)

                                   acgtagcgtcaggagcgacatgagcgacaggAGCGACCGGTCAAGCAGGACGAGCTGGAGCGAGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGCGCTACTGGGGAAGCCGGa                                                                                                     <  1:4862958/136‑1 (MQ=21)

                                       agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg                                                                                                 >  1:4039817/1‑140 (MQ=255)

                                       agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg                                                                                                 >  1:1251716/1‑140 (MQ=255)

                                         ccccaggacccacaggagccaccggagcACCCGGTCAAGCTGGACCACCTGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                               <  1:1860366/137‑1 (MQ=18)

                                            caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  2:1548075/110‑1 (MQ=255)

                                            caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          >  1:1548075/1‑110 (MQ=255)

                                             aggagcgaccggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                           >  2:1054943/1‑140 (MQ=255)

                                                 gcgacaggagctaaaggagcTCCCGGTGAAGCTGGAGCTGCTGTAGCGACGGGAGCCACAGGAGCTACCGGTGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                       <  2:4258239/140‑1 (MQ=21)

                                                  cgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACGGGGGAAGCCGGCCCAGCCGGAGCGAcc                                                                                      >  1:4470341/1‑139 (MQ=255)

                                                    ccaggagcgccaggcgcACCCGGACCACCAGGACCCGCGGGAGCCACGGGAGCCACAGGAGATACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                    <  2:793385/139‑1 (MQ=17)

                                                    acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                    >  1:2505171/1‑140 (MQ=255)

                                                    acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                    >  2:1147295/1‑140 (MQ=255)

                                                    acaggagcgacaggagcAACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCCACGGGAGCCACAGGCGCAACGAGCCAACGCGGAGCAGCCGGAGCGACaag                                                                                    >  2:2221849/1‑138 (MQ=18)

                                                      aggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTCCGGGGGACGACGGACCAGCCGGGGCCACGGGAGCAACAGGGGCGAGGGGGGCGGCGGGACCGGCCGGCGCGCCCGGAg                                                                                  >  2:4433281/1‑140 (MQ=17)

                                                        gagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                <  2:4691655/140‑1 (MQ=255)

                                                        gagcgacacgagcTACCGGCGAAGCTGGACCACCTGCAGCCACGTGAGCGACAGGAGCTCCCGGGGAAGCGCGACCACCCGGTGCCACGGGAGCGACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                <  2:143021/140‑1 (MQ=18)

                                                         agcgacaggagcTCCCGGGGAAGCGGGACCAGCTGGAGCGACGGGAGCGACCGGAGCGACCGGCGAAGCCGGACCAGCGGGGGCGACCGGAGCCACAGGGGCTCCGGGGGACGCCGGACCAGCCGGAGCGCCAGCAGCtc                                                                               >  2:747631/1‑139 (MQ=255)

                                                         agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACTGGAGCTACAGGGGAAGGCGCAGCTGCCTGAGCCTCAGGAGCTa                                                                               >  1:1088637/1‑140 (MQ=18)

                                                         agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                               <  1:3765434/140‑1 (MQ=255)

                                                         agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                               <  1:2852027/140‑1 (MQ=255)

                                                         agcgacaggagcTACCGGGGAAGCTGGACCAGCAGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCtc                                                                               >  1:4706152/1‑139 (MQ=255)

                                                           cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACCGGGGCTACTGGGGAGGGGGGACCCGCCGGGGCGAGAGGAGCtctc                                                                             >  2:44918/1‑137 (MQ=18)

                                                           cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACGGGAGCGCCAGGGGCTACGGGGGACGCCGGCCCAGCCGGAGCGACAGGGGCTAcc                                                                             >  2:4039003/1‑140 (MQ=18)

                                                             acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCgg                                                                           >  1:716876/1‑140 (MQ=255)

                                                             acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCCACAGGCGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAACGCGGAGCACCCGGAGCCACAGCAGCGCCCgg                                                                           >  2:4752247/1‑140 (MQ=18)

                                                                  agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACGGGCGCTACTGGCGATGAGGGACCTGCGGGAGCGACGGGAGATAcccgcgagg                                                                      >  2:4532938/1‑133 (MQ=17)

                                                                  agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGACGGGGCCACGGGAGCCACGGGGGCGACGTGCGAAGCCGGACCAGGCGGAGGGACAGGAGCTACCGGGGAAg                                                                      >  2:3904944/1‑140 (MQ=21)

                                                                  agcTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCAACCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCGACAGGGGCGTCGGGCGAAGCGGGACCGGCCGGAGCGACGGGAGCTACCGGGGacg                                                                      >  2:1884788/1‑138 (MQ=18)

                                                                    cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                                                               >  2:584894/1‑81 (MQ=255)

                                                                      aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTCCTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGCGCAGCCgg                                                                  >  2:4920028/1‑140 (MQ=21)

                                                                      aCCGGAGCAGCCGGACTGGCTGGAGTCACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                  <  1:1800625/140‑1 (MQ=17)

                                                                        cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                <  1:1322936/140‑1 (MQ=255)

                                                                             aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                           <  2:1452280/140‑1 (MQ=255)

                                                                               gcgggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCGGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                         <  1:4801660/137‑1 (MQ=18)

                                                                               ccTGGACCTCGTGCAGCGACCGCACCTACAGGACCTACCGGGGAAGCCTGACCTTCCGTTGCCACGGGAGCCACAGGGGCACCTGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                         <  2:1431955/139‑1 (MQ=14)

                                                                                 tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                        <  2:3855426/75‑1 (MQ=255)

                                                                                 tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACAGGGGAAGCCGGACCAGCCGTAg                                                       >  2:3120830/1‑140 (MQ=255)

                                                                                 tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGCCGGGAGCCACAGGGGCGCCTGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGACGCCGGACCAGCCggcg                                                       >  2:607173/1‑138 (MQ=17)

                                                                                 cggACCCTCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGTGCCACAGGCGCTTCTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                       <  2:561845/139‑1 (MQ=17)

                                                                                 cggACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                       <  1:4592623/139‑1 (MQ=255)

                                                                                 cggACCACCCGGAGCCCCCGGAGCTACAGGCGATCCCGGACCACCCGGAGCCCCCGGAGCCACGGGAGCCACCGGTGCTACTGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGAg                                                       <  2:3933685/139‑1 (MQ=2)

                                                                                        gCTGGAGCGACGGGAGCGACAGGGGCTACGGGGGACGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCAACGGGGGAAGCCGGAGCGGCAGGCGCGACGGGAGCAACCGGGTCAGGCGGAGCGGGCGTAGCGGCatc                                                >  1:5059623/1‑138 (MQ=9)

                                                                                         cTGGAGCGATGGGAGCCCCAGGCGCTACCGGGGAACCCGGACCAGCCGGGGCCACAGGAGCCACAGGGCCTACTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAggc                                               <  1:2914714/140‑2 (MQ=17)

                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGa                                               >  1:4386311/1‑140 (MQ=255)

                                                                                          tGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                              <  1:1119664/140‑1 (MQ=255)

                                                                                                catgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCg                                        >  2:2597528/4‑140 (MQ=255)

                                                                                                 aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACggg                                       >  1:3958137/1‑140 (MQ=255)

                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGCGCACCAGGCGACGCCGGACCACCCGCACCGACAGGCGCGAcgcgg                                      >  2:2790622/1‑136 (MQ=11)

                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                  <  2:3156245/96‑1 (MQ=255)

                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                  >  1:3156245/1‑96 (MQ=255)

                                                                                                   gggAGCGACAGGAGCTACCGGGGAATCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAGCCGGAGCGACAGGAGCTACCgggg                                     >  1:5055473/1‑140 (MQ=255)

                                                                                                               aGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                <  1:261523/101‑1 (MQ=255)

                                                                                                                ccTACCGGCGCACCCGGACCACCCGGTGCCACGGGAGCCACAGGGGCTACGGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                        <  1:2490224/139‑1 (MQ=17)

                                                                                                                        ggAAGCCGGACCCCCCGGGGCAACGGGAGCCACTGGGGCTTCTGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                <  1:3499042/140‑1 (MQ=17)

                                                                                                                         gAAGCCGGAGCCGCCGGGGCCACGGGAGCACCAGGAGCTCCTGGGGAACCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc               <  1:5100672/140‑1 (MQ=14)

                                                                                                                         gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc               >  2:2861136/1‑140 (MQ=255)

                                                                                                                            tccGGACCAGCCGGGGCCACGGGACCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg            <  2:4142210/139‑1 (MQ=255)

                                                                                                                            gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCGACCGGGGACCCCGGCGGAGCCGGAGCGCCAgg            >  2:2700806/1‑140 (MQ=17)

                                                                                                                            gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACAgg            >  2:5088997/1‑140 (MQ=255)

                                                                                                                            cccGGCCCAGCCGGGGACACGGGTGCGACTGGGCCTTCTGGGCTAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg            <  1:2424513/139‑1 (MQ=17)

                                                                                                                                gACCAGCCGGGGCCCCGGGAGCCACCGGGGCTCGGGGTGAAGCGGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc        <  1:3625734/140‑2 (MQ=14)

                                                                                                                                 aCCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa       <  1:1054943/140‑1 (MQ=255)

                                                                                                                                      ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAGCGGAGCGCCAGGAGCGACAggg  >  1:2558818/1‑140 (MQ=255)

                                                                                                                                      ccGCGGCCACCGCAGCCACCCGGGCCACTGGGGAAGCCGGCCCCGCCGGAGCGACAGTAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg  <  2:1925330/140‑1 (MQ=14)

AGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGG  >  NZ_CP034943/607424‑607697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`