Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,565 C→G 9.8% A136A (GCC→GCG)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5650CG 9.8% 321.1 / 12.6 141A136A (GCC→GCG) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base C (64/59);  new base G (13/3);  total (78/63)
Fisher's exact test for biased strand distribution p-value = 3.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

GCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGG  >  NZ_CP034943/607428‑607697
                                                                                                                                          |                                                                                                                                    
gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCCACCGGAGCAACCGGGGCAGCTGGAC‑CAGCGGGAGCCACCGGAGCGACAGGAGCTACCGGGGAACCCGGAGCAGCCGGGGCCAc                                                                                                                                    >  2:4809708/1‑140 (MQ=17)
gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                                                                    >  2:430940/1‑140 (MQ=255)
gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGACCACCCGGGGACGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGGGCGAc                                                                                                                                    >  2:4960287/1‑140 (MQ=18)
   ccaggagcTACCGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:2060874/139‑1 (MQ=255)
   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 >  2:1119664/1‑140 (MQ=255)
   acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:3132458/140‑1 (MQ=255)
   acagcagcACCCGGCGAAGCCCGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCTGGGAAGCTTGAC‑CAGCTGGAGCTACGGTGGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                 <  1:4147513/140‑1 (MQ=18)
     aggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                                                               >  1:3801428/1‑140 (MQ=255)
         gcTCCCGGGGAAGCCGCACCAGCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGATACCTGGGAAGCTGGAC‑CAGTTGTAGCTACTGTAGAGACATGAGCTACCGGGTTAGCAGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                           <  1:1652818/140‑1 (MQ=255)
          cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  1:433196/140‑1 (MQ=255)
          cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  1:2286899/140‑1 (MQ=255)
            cccGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                        <  1:2107229/139‑1 (MQ=255)
            aCCGGGGACGCCGGACCCGGCGGAGGCACCGGTGCCCCAGGCGCGTCCGGAGCAACCGGGCCCCCTGGCC‑CACCGGGAGCCACGGGAGCCACAGGACCAACCGGGGAAGCCGGACCCGCGGGGGCCACGGGAGCCACAgg                                                                                                                        <  2:2157211/140‑1 (MQ=255)
            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGATCGACAGGAGCTACCGGAGAAGCTGGAC‑CAGCTGGAGCGACTGGAGCGACAGGAGCTGCCGGTGCAGCCGGACCAGCCGGGGCGACGGGAGCCGCAgg                                                                                                                        >  1:786610/1‑140 (MQ=18)
              cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                      >  2:3765434/1‑140 (MQ=255)
              cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                      >  2:2852027/1‑140 (MQ=255)
                 ggcaGCCGGACCAGCCGGAGCACCAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                   <  1:4626058/137‑1 (MQ=18)
                 ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTAACGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                   <  1:1619222/140‑1 (MQ=255)
                 ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGCAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                   <  1:4182291/140‑1 (MQ=255)
                  gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCCCGGGAGCCACCGGGGCTAc                                                                                                                  >  2:1938865/1‑140 (MQ=255)
                  gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGATACCGGGCAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGCAGCTACCGCGGAAGCCGGACCACCCCGGGCCACGGGAGCCACCGCGGCGAc                                                                                                                  >  2:4177203/1‑140 (MQ=18)
                     gCCGGACCAGCCGGAGCGACTGGAGCGACAGGAGCCACAGGAGCAACCGGGGAAGCTGGAC‑CACCGGGAGCGACGCGAGCGACACGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACGGGGGCTACggg                                                                                                               >  1:5207337/1‑140 (MQ=17)
                     gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCTACCGTGGCAGATGGACACAAAT‑CCGCGACCGGCGGAACACGACCTAACGGGGAAGCCGGACCAACCGGTGCAACAGGAGCCACGGGGGCTACggg                                                                                                               >  1:4974829/1‑140 (MQ=12)
                        ggACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCCCCGGGGCCACCGGCGCCACAGGGGCTCCTGGGGa                                                                                                            >  2:4013500/1‑140 (MQ=21)
                          aCCCGCCGGCGCGACAGGAGCGACAGGCGCGCCCGGAGCCCGCGGCGCAGCTGGCC‑CAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                          <  1:3846425/140‑1 (MQ=21)
                          aCCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCTCCCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAg                                                                                                          >  2:2884030/1‑140 (MQ=255)
                          aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCCACAgggccag                                                                                                          >  2:4320185/1‑136 (MQ=17)
                               ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCGACAGGGGCTCAGGGGGAAGCCGGa                                                                                                     >  2:1587934/1‑140 (MQ=21)
                               ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                     >  1:4070879/1‑140 (MQ=255)
                                   agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg                                                                                                 >  1:1251716/1‑140 (MQ=255)
                                   agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg                                                                                                 >  1:4039817/1‑140 (MQ=255)
                                     ctacaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                               <  1:2040263/138‑1 (MQ=255)
                                        caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          >  1:1548075/1‑110 (MQ=255)
                                        caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                          <  2:1548075/110‑1 (MQ=255)
                                         aggagcgaccggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                           >  2:1054943/1‑140 (MQ=255)
                                              cgacaggagcgacaggagcTACCGGGGACGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCGGGGCCACGGGAGCCACGGGGGCGACTGGGGCAGCCGGACCCGCCGGCGCGAcg                                                                                      >  2:4113480/1‑139 (MQ=21)
                                              cgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACGGGGGAAGCCGGCCCAGCCGGAGCGAcc                                                                                      >  1:4470341/1‑139 (MQ=255)
                                                ccaggagcgccaggcgcACCCGGACCACCAGGAC‑CCGCGGGAGCCACGGGAGCCACAGGAGATACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                    <  2:793385/139‑1 (MQ=17)
                                                acaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                    >  2:1147295/1‑140 (MQ=255)
                                                acaggagcgacaggagcAACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCCACGGGAGCCACAGGCGCAACGAGCCAACGCGGAGCAGCCGGAGCGACaag                                                                                    >  2:2221849/1‑138 (MQ=18)
                                                  aggagctaaaggagcTCCCGGTCAACCTGGAC‑TAGCGGGAGTGATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACTGGAGCCACTGGCGATACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                  <  1:1727684/140‑1 (MQ=14)
                                                  aggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTCCGGGGGACGACGGACCAGCCGGGGCCACGGGAGCAACAGGGGCGAGGGGGGCGGCGGGACCGGCCGGCGCGCCCGGAg                                                                                  >  2:4433281/1‑140 (MQ=17)
                                                    gagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                <  2:4691655/140‑1 (MQ=255)
                                                    gagcgacacgagcTACCGGCGAAGCTGGAC‑CACCTGCAGCCACGTGAGCGACAGGAGCTCCCGGGGAAGCGCGACCACCCGGTGCCACGGGAGCGACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                <  2:143021/140‑1 (MQ=18)
                                                     ggcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                               >  1:4487107/2‑140 (MQ=255)
                                                     agcgacaggagcTCCCGGGGAAGCGGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCGACCGGCGAAGCCGGACCAGCGGGGGCGACCGGAGCCACAGGGGCTCCGGGGGACGCCGGACCAGCCGGAGCGCCAGCAGCtc                                                                               >  2:747631/1‑139 (MQ=255)
                                                     agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACTGGAGCTACAGGGGAAGGCGCAGCTGCCTGAGCCTCAGGAGCTa                                                                               >  1:1088637/1‑140 (MQ=18)
                                                     agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                               <  1:3765434/140‑1 (MQ=255)
                                                     agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                               <  1:2852027/140‑1 (MQ=255)
                                                     agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCAGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCtc                                                                               >  1:4706152/1‑139 (MQ=255)
                                                       cgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACCGGGGCTACTGGGGAGGGGGGACCCGCCGGGGCGAGAGGAGCtctc                                                                             >  2:44918/1‑137 (MQ=18)
                                                       cgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACGGGAGCGCCAGGGGCTACGGGGGACGCCGGCCCAGCCGGAGCGACAGGGGCTAcc                                                                             >  2:4039003/1‑140 (MQ=18)
                                                         acaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCgg                                                                           >  1:716876/1‑140 (MQ=255)
                                                                cTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                                                               >  2:584894/1‑81 (MQ=255)
                                                                  aCCGGAGCAGCCGGAC‑TGGCTGGAGTCACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                  <  1:1800625/140‑1 (MQ=17)
                                                                    cGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                <  1:1322936/140‑1 (MQ=255)
                                                                       ggAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGCGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGATACCGGGGCAGCCGGAcccg                                                             >  2:4626497/1‑138 (MQ=18)
                                                                        gAAGCGGGAC‑CAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGGCGGACCAGCCGGAGCCACAGGCGCGACAGGGGCGACCGGCCCAGCCGGAGCGGCCGGAGCCAAGGGAGCGACCGGGGAAGCAGGACCAGc                                                            >  2:2325338/1‑140 (MQ=12)
                                                                         cctcTGGAC‑CAGCCGGAGCGACGGGACCCACAGGCGCTACCGGGGAACCCGGACATTCCGGGTCCTCTGGAGACTCCGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                           <  2:1565562/137‑1 (MQ=17)
                                                                         aaGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                           <  2:1452280/140‑1 (MQ=255)
                                                                           gcgggAC‑CAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCGGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                         <  1:4801660/137‑1 (MQ=18)
                                                                           ccTGGAC‑CTCGTGCAGCGACCGCACCTACAGGACCTACCGGGGAAGCCTGACCTTCCGTTGCCACGGGAGCCACAGGGGCACCTGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                         <  2:1431955/139‑1 (MQ=14)
                                                                             tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                        <  2:3855426/75‑1 (MQ=255)
                                                                             tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACAGGGGAAGCCGGACCAGCCGTAg                                                       >  2:3120830/1‑140 (MQ=255)
                                                                             tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGCCGGGAGCCACAGGGGCGCCTGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGACGCCGGACCAGCCggcg                                                       >  2:607173/1‑138 (MQ=17)
                                                                             cggAC‑CCTCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGTGCCACAGGCGCTTCTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                       <  2:561845/139‑1 (MQ=17)
                                                                             cggAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                       <  1:4592623/139‑1 (MQ=255)
                                                                             cggAC‑CAGCCGGAGCGACGGGAGCCACGGGAGCGACCGGTGAAGCGGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                       <  2:2830459/139‑1 (MQ=11)
                                                                             cggAC‑CACCCGGAGCCCCCGGAGCTACAGGCGATCCCGGACCACCCGGAGCCCCCGGAGCCACGGGAGCCACCGGTGCTACTGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGAg                                                       <  2:3933685/139‑1 (MQ=2)
                                                                              ggAC‑CAGCGGGAGCGACGGGAGCGACAGGCGCTACTGGGGAAGGCGGACCAGCCGGTGCCACGGGATCAACTGGGGCATCGGTGGACGCCGGACACGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccggccggggg                                                      >  1:2146254/1‑137 (MQ=17)
                                                                               gAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg                                                     >  2:1879204/1‑140 (MQ=255)
                                                                                     gCTGGAGCGACGGGAGCGACAGGGGCTACGGGGGACGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCAACGGGGGAAGCCGGAGCGGCAGGCGCGACGGGAGCAACCGGGTCAGGCGGAGCGGGCGTAGCGGCatc                                                >  1:5059623/1‑138 (MQ=9)
                                                                                      cTGGAGCGATGGGAGCCCCAGGCGCTACCGGGGAACCCGGACCAGCCGGGGCCACAGGAGCCACAGGGCCTACTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAggc                                               <  1:2914714/140‑2 (MQ=17)
                                                                                       tGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                              <  1:1119664/140‑1 (MQ=255)
                                                                                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                           >  1:2556866/1‑140 (MQ=255)
                                                                                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                           >  2:479439/1‑140 (MQ=255)
                                                                                             catgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCg                                        >  2:2597528/4‑140 (MQ=255)
                                                                                              aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACggg                                       >  1:3958137/1‑140 (MQ=255)
                                                                                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGCGCACCAGGCGACGCCGGACCACCCGCACCGACAGGCGCGAcgcgg                                      >  2:2790622/1‑136 (MQ=11)
                                                                                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                  >  1:3156245/1‑96 (MQ=255)
                                                                                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCGACAGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGCACAGACAGGAGCGAGCggt                                      >  2:3900707/1‑139 (MQ=14)
                                                                                                gggAGCGACAGGAGCTACCGGGGAATCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAGCCGGAGCGACAGGAGCTACCgggg                                     >  1:5055473/1‑140 (MQ=255)
                                                                                                gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCggcg                                     >  2:4339681/1‑138 (MQ=255)
                                                                                                   aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                  <  1:479439/140‑1 (MQ=255)
                                                                                                            aGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                >  2:261523/1‑101 (MQ=255)
                                                                                                            aGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                <  1:261523/101‑1 (MQ=255)
                                                                                                             gCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                        <  1:2490177/140‑1 (MQ=255)
                                                                                                             ccTACCGGCGCACCCGGACCACCCGGTGCCACGGGAGCCACAGGGGCTACGGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                        <  1:2490224/139‑1 (MQ=17)
                                                                                                                     ggAAGCCGGACCCCCCGGGGCAACGGGAGCCACTGGGGCTTCTGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                <  1:3499042/140‑1 (MQ=17)
                                                                                                                      gAAGCCGGAGCCGCCGGGGCCACGGGAGCACCAGGAGCTCCTGGGGAACCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc               <  1:5100672/140‑1 (MQ=14)
                                                                                                                      gAAGCCGGACCAGCCGGTGCCACGGGAGCAACAGGGGCTACTGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc               <  1:4369967/140‑1 (MQ=255)
                                                                                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGCACGGACAGGCGCTACCGGGGACGCCGGACCAACCGGAGAGACAGGAGCAACAGTGGAAGCGGGGCCAGGCGGTGCGAc               >  2:3110344/1‑140 (MQ=14)
                                                                                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc               >  2:2861628/1‑140 (MQ=255)
                                                                                                                         tccGGACCAGCCGGGGCCACGGGACCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg            <  2:4142210/139‑1 (MQ=255)
                                                                                                                         gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGCCCGGGGAAGCCGGACCCACCGGAGCGACggg            >  2:4144026/1‑140 (MQ=17)
                                                                                                                         gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACAgg            >  2:5088997/1‑140 (MQ=255)
                                                                                                                             gACCAGCCGGGGCCCCGGGAGCCACCGGGGCTCGGGGTGAAGCGGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc        <  1:3625734/140‑2 (MQ=14)
                                                                                                                              aCCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa       <  1:1054943/140‑1 (MQ=255)
                                                                                                                                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAGCGGAGCGCCAGGAGCGACAggg  >  1:2558818/1‑140 (MQ=255)
                                                                                                                                   ccGCGGCCACCGCAGCCACCCGGGCCACTGGGGAAGCCGGCCCCGCCGGAGCGACAGTAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg  <  2:1925330/140‑1 (MQ=14)
                                                                                                                                          |                                                                                                                                    
GCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGG  >  NZ_CP034943/607428‑607697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑