BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,638	G→C	9.5%	A161P (GCC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,638	0	G	C	9.5%	234.2 / 6.3	116	A161P (GCC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (55/45); new base C (5/7); total (62/54)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607500‑607777

gAAGCGGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGGCGGACCAGCCGGAGCCACAGGCGCGACAGGGGCGACCGGCCCAGCCGGAGCGGCCGGAGCCAAGGGAGCGACCGGGGAAGCAGGACCAGc                                                                                                                                            >  2:2325338/1‑140 (MQ=12)

 cctcTGGACCAGCCGGAGCGACGGGACCCACAGGCGCTACCGGGGAACCCGGACATTCCGGGTCCTCTGGAGACTCCGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                           <  2:1565562/137‑1 (MQ=17)

 aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                           <  2:1452280/140‑1 (MQ=255)

   gcgggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCGGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         <  1:4801660/137‑1 (MQ=18)

   ccTGGACCTCGTGCAGCGACCGCACCTACAGGACCTACCGGGGAAGCCTGACCTTCCGTTGCCACGGGAGCCACAGGGGCACCTGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         <  2:1431955/139‑1 (MQ=14)

     tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACAGGGGAAGCCGGACCAGCCGTAg                                                                                                                                       >  2:3120830/1‑140 (MQ=255)

     tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGCCGGGAGCCACAGGGGCGCCTGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGACGCCGGACCAGCCggcg                                                                                                                                       >  2:607173/1‑138 (MQ=17)

     cggACCCTCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGTGCCACAGGCGCTTCTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                       <  2:561845/139‑1 (MQ=17)

     cggACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                       <  1:4592623/139‑1 (MQ=255)

     cggACCAGCCGGAGCGACGGGAGCCACGGGAGCGACCGGTGAAGCGGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                       <  2:2830459/139‑1 (MQ=11)

      ggACCAGCGGGAGCGACGGGAGCGACAGGCGCTACTGGGGAAGGCGGACCAGCCGGTGCCACGGGATCAACTGGGGCATCGGTGGACGCCGGACACGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccggccggggg                                                                                                                                      >  1:2146254/1‑137 (MQ=17)

            gCTGGAGCGACGGGAGCGACAGGGGCTACGGGGGACGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCAACGGGGGAAGCCGGAGCGGCAGGCGCGACGGGAGCAACCGGGTCAGGCGGAGCGGGCGTAGCGGCatc                                                                                                                                >  1:5059623/1‑138 (MQ=9)

             cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGa                                                                                                                               >  1:4386311/1‑140 (MQ=255)

              tGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                              <  1:1119664/140‑1 (MQ=255)

                 aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                           >  2:479439/1‑140 (MQ=255)

                    catgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCg                                                                                                                        >  2:2597528/4‑140 (MQ=255)

                     aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACggg                                                                                                                       >  1:3958137/1‑140 (MQ=255)

                      cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGCGCACCAGGCGACGCCGGACCACCCGCACCGACAGGCGCGAcgcgg                                                                                                                      >  2:2790622/1‑136 (MQ=11)

                      cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCGACAGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGCACAGACAGGAGCGAGCggt                                                                                                                      >  2:3900707/1‑139 (MQ=14)

                       gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCggcg                                                                                                                     >  2:4339681/1‑138 (MQ=255)

                                    gCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                                        <  1:2490177/140‑1 (MQ=255)

                                    ccTACCGGCGCACCCGGACCACCCGGTGCCACGGGAGCCACAGGGGCTACGGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                                        <  1:2490224/139‑1 (MQ=17)

                                       aCCGGGGGAGCCGGACCAGCCGGCGCCAGGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGGGAGGCCGGGACAACCgc                                                                                                     >  2:143672/1‑139 (MQ=17)

                                            ggAAGCCGGACCCCCCGGGGCAACGGGAGCCACTGGGGCTTCTGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                                <  1:3499042/140‑1 (MQ=17)

                                             gAAGCCGGAGCCGCCGGGGCCACGGGAGCACCAGGAGCTCCTGGGGAACCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                               <  1:5100672/140‑1 (MQ=14)

                                             gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                               >  2:2861136/1‑140 (MQ=255)

                                             gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGCACGGACAGGCGCTACCGGGGACGCCGGACCAACCGGAGAGACAGGAGCAACAGTGGAAGCGGGGCCAGGCGGTGCGAc                                                                                               >  2:3110344/1‑140 (MQ=14)

                                                tccGGACCAGCCGGGGCCACGGGACCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                            <  2:4142210/139‑1 (MQ=255)

                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCGACCGGGGACCCCGGCGGAGCCGGAGCGCCAgg                                                                                            >  2:2700806/1‑140 (MQ=17)

                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGCCCGGGGAAGCCGGACCCACCGGAGCGACggg                                                                                            >  2:4144026/1‑140 (MQ=17)

                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACAgg                                                                                            >  2:5088997/1‑140 (MQ=255)

                                                cccGGCCCAGCCGGGGACACGGGTGCGACTGGGCCTTCTGGGCTAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                            <  1:2424513/139‑1 (MQ=17)

                                                     aCCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                                                                       <  1:1054943/140‑1 (MQ=255)

                                                          ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAGCGGAGCGCCAGGAGCGACAggg                                                                                  >  1:2558818/1‑140 (MQ=255)

                                                          ccGGGGACCCGGGTCCCACAGGTGCTACTGGCGAAGCGGGACCCCCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                  <  1:1186144/140‑1 (MQ=14)

                                                          ccGCGGCCACCGCAGCCACCCGGGCCACTGGGGAAGCCGGCCCCGCCGGAGCGACAGTAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                  <  2:1925330/140‑1 (MQ=14)

                                                                   cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCTCCCGCAGCGCCCGGAGCGCCCGGGGACTCCGGa                                                                         >  1:3572927/1‑140 (MQ=14)

                                                                    gggAGCCCCAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGAGCGACCGGAGCGACCGGGGCCGCGGGCCCCCCCGGAGCGAGAGGAGCTCGCGGGGCACCCgggc                                                                        >  2:4361215/1‑138 (MQ=14)

                                                                    gggAGCCACAGGGGCTGCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGACGCGGGCCCAGCAGGAGCGGCGGTAGCGAACGGGGCGGCCGGAc                                                                        >  2:828872/1‑140 (MQ=11)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGTAGCTACCGGGGAAGCCGGAc                                                                        >  2:1697417/1‑140 (MQ=255)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                        <  1:3872058/140‑1 (MQ=255)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                        <  1:3681783/140‑1 (MQ=255)

                                                                         ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                        >  1:4586593/1‑135 (MQ=255)

                                                                         ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                        <  2:4586593/135‑1 (MQ=255)

                                                                             aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                               <  1:2861628/140‑1 (MQ=255)

                                                                             aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                               <  1:2861136/140‑1 (MQ=255)

                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                           >  2:2591270/1‑140 (MQ=40)

                                                                                 cctcatGGGCAAGCCGGACCACCCGGAGCCACAGGCGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                           <  1:3206347/135‑1 (MQ=11)

                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCACCCGCAGCGGCGGGAGCGACCGGGGAAACAGCACCAGCCGGAGCGCCa                                                          >  2:3576086/1‑140 (MQ=11)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGAAGCTACCGGGGCCGCCGGCCCAGCCGGAGCGACAgg                                                        >  2:2677029/1‑140 (MQ=14)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                                        >  1:2961880/1‑60 (MQ=255)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                                        >  1:1840287/1‑60 (MQ=255)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAAACGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGACGGAGCTACAgg                                                        >  2:4609251/1‑140 (MQ=14)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                               <  1:5113126/101‑1 (MQ=35)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                               >  2:5113126/1‑101 (MQ=35)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                        <  1:2591270/140‑1 (MQ=255)

                                                                                         ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                  <  1:5040038/109‑1 (MQ=35)

                                                                                          gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGAGACGCCGGAGCAGCCGGAGCGACAGGAGCGAc                                                  >  1:1640291/1‑140 (MQ=14)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCAACAGCGGATACAGGACAACCCGGAGCTGCCGGAGCGACAGGCGCACCCGGACACGCCGCAGCGACAGGAGCTAcc                                                 >  1:3521006/1‑140 (MQ=9)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAACCGCAGCGCCCGGAGCAACCGGGGAAGCCGGACAAGCCGGACCCACACCAGCTAcc                                                 >  1:3748512/1‑140 (MQ=14)

                                                                                             gCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                               <  1:2884030/140‑1 (MQ=255)

                                                                                             gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCCGGAGcggcagg                                               >  2:2570777/1‑134 (MQ=11)

                                                                                               cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACCGGAGCTACCgggg                                             >  2:2208822/1‑140 (MQ=255)

                                                                                                  aCCCGCCGGACCCACCGGAGCTACCGGAGCCCCAGGACCACCCGGAGCCTCAGGACCTCCCGGGGAGCCAGGACCTACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAg                                          <  1:4447550/140‑1 (MQ=9)

                                                                                                  aCCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                          <  1:29943/140‑1 (MQ=255)

                                                                                                  aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                          <  1:1990038/140‑1 (MQ=255)

                                                                                                  aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                          <  1:4809708/140‑1 (MQ=255)

                                                                                                    cccccGGAGCTCCAGGCGCTACGGGAGAAGCCGGACCACCCGGAGCCACCGGAGCGCCCGGGGCCCCCGGACCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGcc                                        <  2:2391088/137‑1 (MQ=11)

                                                                                                       ccGGAGCGACCGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGA                                     <  2:4070879‑M1/140‑1 (MQ=255)

                                                                                                       caggAGCCACCGGAGCACCCGGCGCCCCCGGAGCACCCGGACCCACAGGACCTACCGGCGACCCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                     <  2:4253755/138‑1 (MQ=11)

                                                                                                                   gAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                         <  2:2556866/140‑1 (MQ=255)

                                                                                                                     gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGGCAGGAGCCACCGGGGCAGCCGGCGAAGCCGGAGCGACACGAGCGACCGGGGAAGCCGGACCCtccggagcgcc                       >  2:2417788‑M1/1‑129 (MQ=255)

                                                                                                                        aCCGGGGATGCCGGAGCACCCGGCGCTCCCGGAGCTACCGGGGCACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                    <  1:4532527/140‑1 (MQ=14)

                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                    >  2:4135486/1‑140 (MQ=255)

                                                                                                                          cGGGGACGCCGGACCCTCCGGTGCGACTGGAGCCCCCGGCGACGCCCGACCAACCGGAGCCACAGGCGCTACCGGCGACGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                  <  1:1245429/140‑1 (MQ=11)

                                                                                                                          cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGTGCGCCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAg                  >  2:3607661/1‑140 (MQ=11)

                                                                                                                          cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACAGGAGCGACAGGATCTACCTGGGAATCCGGACCAGACCGAGCGACAGGAGCTAACGGGGAAGCAGTACCAAACGGAGCGACGGGAg                  >  1:3339297/1‑140 (MQ=14)

                                                                                                                          cGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGTCCAAACGGAGCGACAGGAGCGACAGGGGAAGCCGGGGCAGCCGGACCGACAGGAGCTACCGGGGAACCCGGCGCAACCGGGGCGACCGGAg                  >  2:515065/1‑140 (MQ=11)

                                                                                                                            gcgacgCCGGCCCACCCGGAGCCCCTGGAGCCCCCGGGGCAGCCGGCCCAACCGGTGCCCCAGGACCAACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                <  1:2058065/135‑1 (MQ=14)

                                                                                                                              gcaGCAGGACCAGCCGGAGCTCCCGGAGCAACCGGCGAACCCGGACCAACCGGAGCCCCAGGACCACCCGGGGAACCCGGAGCACCGGGAGCCACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  1:1208980/138‑1 (MQ=12)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCGACCGGGGCCGCCGGACCAGCCGGAGCGCGAGGAGCGCCCGGCGCGGCCGGAGCAACCGGAGCGACGGGAGcccg              >  2:480129/1‑138 (MQ=14)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              >  2:3931396/1‑140 (MQ=255)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACTCCGGACCAGCCGGAGCGCCAGTAGCGACCTGCGACTCCGGACCAACCGGAGCGACGGGAGCgac              >  2:3514235/1‑140 (MQ=14)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACGGCAGCgac              >  2:3498844/1‑140 (MQ=255)

                                                                                                                               caGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca             <  1:1600747/139‑1 (MQ=255)

                                                                                                                               actccGGACCAGCCGGCGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGCGCTCCCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca             <  2:3801428/137‑1 (MQ=14)

                                                                                                                               aaGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACGGGAGCcaca             >  1:4804990/1‑140 (MQ=255)

                                                                                                                               aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCccca             >  1:3222750/1‑140 (MQ=255)

                                                                                                                                aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGAAGCCGGAGCGACCGGATCGCCCGGGGACGCGGGACCAACCGGAGCGACGGGAGcaccag            >  2:1569158/1‑135 (MQ=14)

                                                                                                                                 gCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  1:4013500/140‑1 (MQ=255)

                                                                                                                                   cGGACCCGCCGGCGCGCCCGGAGCGCCGGGCGCCGCCGGCCCAACCGGAGCGCCAGGAGCCACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACGGGAGCCACAgggg         <  2:4805790/140‑1 (MQ=14)

                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTCCCGGGGCGGCCGGACCACGCGGAGCGCCGGGAGccccgggag         >  2:96712/1‑133 (MQ=11)

                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                       >  2:161841/1‑126 (MQ=38)

                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                       <  1:161841/126‑1 (MQ=38)

                                                                                                                                      aCCCGCCGCCGCCACCGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGACCGGCGCAACCGGCGACGCCGGACCCGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa      <  2:4310815/140‑1 (MQ=14)

                                                                                                                                      aCCAGCCGGATCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                              >  1:340491/1‑68 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  2:340491/68‑1 (MQ=35)

                                                                                                                                          gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAACCGGAGCGGCGGGAGCCACAGGGGCGACggg  >  2:4822117/1‑140 (MQ=14)

                                                                                                                                          gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCCGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCCGCCGGCCCACCAGGGGCGGCGGGAGCCACGGGGGcgccggg  >  2:5075531/1‑134 (MQ=14)

                                                                                                                                          gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCGGAGCGACATGAGCAACAGGGGAACCCGGACAAACAGGAGCCACGGGAGCCACAGGGGCTACCgg  >  2:883434/1‑140 (MQ=17)

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607500‑607777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`