BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	16.7%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	16.7%	294.3 / 33.8	172	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (89/49); new base C (25/7); total (115/57)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607567‑607839

 gggAGCCACAGGGGCTGCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGACGCGGGCCCAGCAGGAGCGGCGGTAGCGAACGGGGCGGCCGGAc                                                                                                                                      >  2:828872/1‑140 (MQ=11)

 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                      <  1:3872058/140‑1 (MQ=255)

      ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                      >  1:4586593/1‑135 (MQ=255)

          aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                             <  1:2861136/140‑1 (MQ=255)

              gctccgggggAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                         <  1:3255742/134‑1 (MQ=255)

              cctcatGGGCAAGCCGGACCACCCGGAGCCACAGGCGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                         <  1:3206347/135‑1 (MQ=11)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCACCCGCAGCGGCGGGAGCGACCGGGGAAACAGCACCAGCCGGAGCGCCa                                                                                                                        >  2:3576086/1‑140 (MQ=11)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                               >  1:2054575/1‑140 (MQ=255)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGCACCAACCGCAGCGACAGCAGCGAACGGGGACGCCGCACCAGCCTGAGCAACAGGAGCTAcc                                                                                                               >  2:1447596/1‑140 (MQ=14)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGAGAACCCGGAACAACCGGAGCGACCGGAGCGAAAGCGGAAGCCGGACAAGACGTATCGCCTGGAGCTccc                                                                                                               >  1:1491649/1‑140 (MQ=11)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAACCGCAGCGCCCGGAGCAACCGGGGAAGCCGGACAAGCCGGACCCACACCAGCTAcc                                                                                                               >  1:3748512/1‑140 (MQ=14)

                          gCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                             <  1:2884030/140‑1 (MQ=255)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCCGGAGcggcagg                                                                                                             >  2:2570777/1‑134 (MQ=11)

                            cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACCGGAGCTACCgggg                                                                                                           >  2:2208822/1‑140 (MQ=255)

                               aCCCGCCGGACCCACCGGAGCTACCGGAGCCCCAGGACCACCCGGAGCCTCAGGACCTCCCGGGGAGCCAGGACCTACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAg                                                                                                        <  1:4447550/140‑1 (MQ=9)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                        <  1:29943/140‑1 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                        <  1:4809708/140‑1 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                        <  1:1990038/140‑1 (MQ=255)

                                 cccccGGAGCTCCAGGCGCTACGGGAGAAGCCGGACCACCCGGAGCCACCGGAGCGCCCGGGGCCCCCGGACCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGcc                                                                                                      <  2:2391088/137‑1 (MQ=11)

                                  aGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGCAGCCG                                                                                                     >  2:460771‑M1/1‑140 (MQ=255)

                                    caggAGCCACCGGAGCACCCGGCGCCCCCGGAGCACCCGGACCCACAGGACCTACCGGCGACCCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                   <  2:4253755/138‑1 (MQ=11)

                                                  gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGGCAGGAGCCACCGGGGCAGCCGGCGAAGCCGGAGCGACACGAGCGACCGGGGAAGCCGGACCCtccggagcgcc                                                                                     >  2:2417788‑M1/1‑129 (MQ=255)

                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                  >  2:4135486/1‑140 (MQ=255)

                                                       cGGGGACGCCGGACCCTCCGGTGCGACTGGAGCCCCCGGCGACGCCCGACCAACCGGAGCCACAGGCGCTACCGGCGACGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                                <  1:1245429/140‑1 (MQ=11)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGTGCGCCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAg                                                                                >  2:3607661/1‑140 (MQ=11)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACAGGAGCGACAGGATCTACCTGGGAATCCGGACCAGACCGAGCGACAGGAGCTAACGGGGAAGCAGTACCAAACGGAGCGACGGGAg                                                                                >  1:3339297/1‑140 (MQ=14)

                                                       cGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGTCCAAACGGAGCGACAGGAGCGACAGGGGAAGCCGGGGCAGCCGGACCGACAGGAGCTACCGGGGAACCCGGCGCAACCGGGGCGACCGGAg                                                                                >  2:515065/1‑140 (MQ=11)

                                                           gcaGCAGGACCAGCCGGAGCTCCCGGAGCAACCGGCGAACCCGGACCAACCGGAGCCCCAGGACCACCCGGGGAACCCGGAGCACCGGGAGCCACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:1208980/138‑1 (MQ=12)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCGACCGGGGCCGCCGGACCAGCCGGAGCGCGAGGAGCGCCCGGCGCGGCCGGAGCAACCGGAGCGACGGGAGcccg                                                                            >  2:480129/1‑138 (MQ=14)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  2:3931396/1‑140 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  2:2670640/1‑140 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACTCCGGACCAGCCGGAGCGCCAGTAGCGACCTGCGACTCCGGACCAACCGGAGCGACGGGAGCgac                                                                            >  2:3514235/1‑140 (MQ=14)

                                                            caGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                           <  1:1600747/139‑1 (MQ=255)

                                                            aaGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACGGGAGCcaca                                                                           >  1:4804990/1‑140 (MQ=255)

                                                              gCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:4013500/140‑1 (MQ=255)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                     >  2:161841/1‑126 (MQ=38)

                                                                   aCCCGCCGCCGCCACCGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGACCGGCGCAACCGGCGACGCCGGACCCGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                    <  2:4310815/140‑1 (MQ=14)

                                                                   aCCAGCCGGATCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                            >  1:340491/1‑68 (MQ=255)

                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                            <  2:340491/68‑1 (MQ=35)

                                                                       gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAACCGGAGCGGCGGGAGCCACAGGGGCGACggg                                                                >  2:4822117/1‑140 (MQ=14)

                                                                       gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCCGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCCGCCGGCCCACCAGGGGCGGCGGGAGCCACGGGGGcgccggg                                                                >  2:5075531/1‑134 (MQ=14)

                                                                       gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCGGAGCGACATGAGCAACAGGGGAACCCGGACAAACAGGAGCCACGGGAGCCACAGGGGCTACCgg                                                                >  2:883434/1‑140 (MQ=17)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                            >  1:5121604/1‑91 (MQ=37)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                           >  1:604654/1‑140 (MQ=255)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                      >  1:1963065/1‑140 (MQ=255)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCGACCGGGGAAGCCGGACCAACCGGAGCCACGGGAGCCACAGCTCCACCTGGGGACGTCGGa                                                      >  1:2838548/1‑140 (MQ=17)

                                                                                  cggAGCTACCGGCGCAGCCGGACCCACCGGCGCGCCAGGAGCGTCGGGGGCCCCCGGTGCAGCCGGAGCGACAGGCGATACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                     <  1:2474634/139‑1 (MQ=18)

                                                                                  cggAGCTACCGGCGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                     <  1:4148361/139‑1 (MQ=255)

                                                                                  cggAGCCACCGGGGCCCCCGGACCAACCGGAGAGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                     <  1:3136294/139‑1 (MQ=18)

                                                                                  aGGAGCTACCGGCCAAGCCGGACCACCCGGCGCGACAGGCGCGTCCGGGGAACCAGGACCAGCCGGAGCGACAGGAGAAACCGGCCAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                     <  1:2594804/140‑1 (MQ=18)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAGCCGGAGCGAGAGGATCTACCGGGGAAGCAGGCGCAACCGGACCGACGGTAGCGCCAGGCGCTACTGTGTAGGTCGGAGCAGCAgg                                              >  2:3024192/1‑140 (MQ=14)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                              >  1:1058453/1‑140 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCgg                                              >  1:261786/1‑140 (MQ=255)

                                                                                               gcccccGGACCCACCGGAGCCACAGCAGCTACCGGGGACCCCGGAGCACCGGGAGCCACTGGAGCTACCGGGGAAGCCGGACATACGGGAGGGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        <  1:733346/136‑1 (MQ=18)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGCGAACCCGGCCCACCCGGAGCGTCGGGCGCGACAGGGGCTAGGGGGGCCCTCGGACCAGCGGGAGCgcc                                        >  1:3569193/1‑138 (MQ=17)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        >  1:68880/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGAc                                        >  2:2085822/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCTACCGGCGAACCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        >  1:4287651/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        <  1:1219261/140‑1 (MQ=255)

                                                                                                cacccGGCCCACCCGGCCCGACCGGAGCTACCGGGGAAGCCGCACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACa                                       <  1:517901/137‑1 (MQ=18)

                                                                                                aaGCCGGACCAACCGGAGCTACAGGTGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACa                                       <  1:2354923/140‑1 (MQ=255)

                                                                                                aaGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACa                                       <  2:4039817/140‑1 (MQ=255)

                                                                                                 aGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAg                                      >  2:3031612/1‑140 (MQ=255)

                                                                                                 aGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAg                                      >  2:2233769/1‑140 (MQ=255)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGCCCTGGGAAGGCGGAGCAACCTGAGCGTCGCGAGCCGCAGCGGGTTCTGGTGAGGTCGGACACGCCGTCGCGACTggtg                                   >  1:2388551/1‑138 (MQ=9)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAACCGGAGCGACGGGAGCCACAGGCGCGACGGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                   >  2:71057/1‑140 (MQ=255)

                                                                                                       cgcgaCCCGAGCCACAGGACCTCCCGGCGAACCCGGACCCGCCGGAGCGACATCAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                <  2:2189921/136‑1 (MQ=17)

                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGt                                                          >  2:4337323/1‑114 (MQ=255)

                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCtc                                >  1:3583409/1‑139 (MQ=255)

                                                                                                       aCCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                <  1:1687891/140‑1 (MQ=255)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                            >  1:2202505/1‑140 (MQ=255)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCTGCCGTAGCGACAGGAGCTACCgg                            >  1:1819316/1‑140 (MQ=255)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                       >  2:776574/1‑140 (MQ=255)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACAGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGAATCGACAGGAGCTACCGGTGAAg                       >  2:2335908/1‑140 (MQ=21)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACAACCCGGAGCGACAGGAGCTAACGCGGAAGCCgg                   >  1:2778176/1‑140 (MQ=21)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAgccggagcgacaggagctaccggggaagccggaccaaccggagcgacaggagccacgggagccacaggcacaggagc                   >  1:101911‑M1/1‑63 (MQ=255)

                                                                                                                     cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACGGGAGCGACAGGGGCTACTGGGGACGTCGGAGCAGCCGGAGCGCCAGGAGCGACCGGGGCAGCCGGa                  >  2:1353118/1‑140 (MQ=18)

                                                                                                                     cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAATCCGGACCAACCTGAGCGACGGGAGCCACCGGGGCCCCAGGCGACGTCGGACCAGCCGGAGCGACAGGCGCAACCGGTGAACCCGGa                  >  2:1041615/1‑140 (MQ=18)

                                                                                                                         ggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa              >  1:1285560/2‑140 (MQ=255)

                                                                                                                         ggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa              >  1:1676799/2‑140 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc             >  2:467541/1‑140 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCacc             >  2:3170684/1‑138 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc             >  1:427364/1‑140 (MQ=255)

                                                                                                                           cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc            >  2:1962729/1‑140 (MQ=255)

                                                                                                                           cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc            >  1:3863492/1‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:3131835/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  2:4709055/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  2:863762/4‑140 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:2074065/1‑140 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:1638829/1‑140 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:4984920/1‑140 (MQ=255)

                                                                                                                               tggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg        >  2:252117/2‑140 (MQ=255)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAgccggagcgacagg                                                                                  >  2:5031665‑M1/1‑48 (MQ=255)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc    >  1:4392852/1‑140 (MQ=255)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGATACTGGGGACGTCGGACCAGCCGGAGCGCCATGAGCTACCGGGGGAGCCGGACCAACCGGAGCGag    >  2:976018/1‑139 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGCAGCGAcgg  >  2:3393115/1‑138 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGCGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGAcgg  >  2:4395811/1‑138 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcgg  >  2:5117020/1‑138 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcgg  >  2:4567112/1‑138 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAcgg  >  2:306576/1‑138 (MQ=255)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607567‑607839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`