Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 44,870 C→T 21.0% intergenic (‑51/‑450) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8700CT21.0% 378.3 / 95.8 176intergenic (‑51/‑450)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (61/78);  new base T (14/23);  total (75/101)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01

Only 100 of 176 total aligned reads displayed.

TGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAA  >  NZ_CP034943/44733‑44988
                                                                                                                                         |                                                                                                                      
tGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGt                                                                                                                      <  1:4358807/140‑1 (MQ=255)
tGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGt                                                                                                                      <  1:2461680/140‑1 (MQ=255)
            agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                          <  2:1112167/140‑1 (MQ=255)
            agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                          <  2:2515735/140‑1 (MQ=255)
            agTACGACGCAATTCGATAGGAAGAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAACCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                          <  2:406735/140‑1 (MQ=255)
             gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTcaacaa                                                                                                         <  1:1463565/140‑1 (MQ=255)
             gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACaa                                                                                                         <  2:1147954/140‑1 (MQ=255)
               acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTGTAAATTATATTTTTCTTCGTCATTATTCAACaaaa                                                                                                       >  1:2917085/1‑140 (MQ=255)
                   cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   >  2:1347387/1‑140 (MQ=255)
                   cgCAATTCGATAGGAATAACTACACGTCCTAGCTCAACAACTTTACGTACACTACCCGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   <  1:1077863/140‑1 (MQ=255)
                    gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                  <  1:4769607/140‑1 (MQ=255)
                    gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGGCATTATTCACCAAAATCCtt                                                                                                  <  1:804301/140‑1 (MQ=255)
                    gCAATTCGATAGGAATAACTAAACGTCATAGCTCTTCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGTTTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                  <  1:5201197/140‑1 (MQ=255)
                      aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                <  1:3692386/140‑1 (MQ=255)
                      aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                <  1:1777872/140‑1 (MQ=255)
                      aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                >  1:4115949/1‑140 (MQ=255)
                      aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAc                                                                                                >  1:3334217/1‑140 (MQ=255)
                           gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCa                                                                                           <  2:2074132/140‑1 (MQ=255)
                           gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                           <  2:1001781/140‑1 (MQ=255)
                           gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                           <  2:2247190/140‑1 (MQ=255)
                           gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                           <  2:4686257/140‑1 (MQ=255)
                                gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   <  2:1933964/127‑1 (MQ=255)
                                gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                   >  1:1933964/1‑127 (MQ=255)
                                gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCa                                                                                      >  1:1433192/1‑140 (MQ=255)
                                    aaCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  <  2:3287539/140‑1 (MQ=255)
                                         cacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGt                                                                             >  2:804289/1‑140 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  <  1:4274950/134‑1 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  <  1:2733668/134‑1 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  >  2:4274950/1‑134 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                  >  2:1835266/1‑134 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:3603104/1‑140 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  1:5075648/1‑140 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:1604141/1‑140 (MQ=255)
                                          acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                            >  2:587669/1‑140 (MQ=255)
                                           cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCTACAAAATCCTTACCTTCATAGCATTAGAAGGttt                                                                           >  2:2334116/1‑140 (MQ=255)
                                           cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  1:166610/1‑140 (MQ=255)
                                           cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  1:1540472/1‑140 (MQ=255)
                                           cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  2:1296198/1‑140 (MQ=255)
                                           cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                           >  1:4560116/1‑140 (MQ=255)
                                           cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTAATAGCATAACAAGGttt                                                                           >  2:3703990/1‑140 (MQ=255)
                                              ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                        >  1:3538335/1‑140 (MQ=255)
                                              ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                        >  2:4401469/1‑140 (MQ=255)
                                              ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTACa                                                                        >  2:4264438/1‑140 (MQ=255)
                                                tAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaat                                                                      <  2:2447047/140‑1 (MQ=255)
                                                      atcaACTTTACGTACAATACCAGTAGATTTCGTAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:3487153/1‑140 (MQ=255)
                                                      atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  1:1791522/1‑140 (MQ=255)
                                                      atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:5204472/1‑140 (MQ=255)
                                                      atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                >  2:3171776/1‑140 (MQ=255)
                                                        caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAAGCGTCa                                                              >  1:1703601/1‑140 (MQ=255)
                                                              tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                        >  2:659982/1‑140 (MQ=255)
                                                                  tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCGTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                    <  1:3154060/140‑1 (MQ=255)
                                                                   aaaaTACCAGTAGATTTCATAAACGTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:1943459/138‑1 (MQ=255)
                                                                   aCAATACCATTAGATTTCATAAAATTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:1091197/140‑1 (MQ=255)
                                                                   aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:4115949/140‑1 (MQ=255)
                                                                   aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:2863015/140‑1 (MQ=255)
                                                                   aCAATACCAGTAGATTTCATAAACGTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                   <  2:385481/140‑1 (MQ=255)
                                                                            gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                          <  1:1634776/140‑1 (MQ=255)
                                                                            gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                          >  1:3742013/1‑140 (MQ=255)
                                                                               gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAGCAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgattg                                       >  1:2720302/1‑140 (MQ=255)
                                                                                aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                      <  2:1539520/140‑1 (MQ=255)
                                                                                aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                      <  2:166610/140‑1 (MQ=255)
                                                                                 tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:3487359/140‑1 (MQ=255)
                                                                                 tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTACTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  2:2529416/140‑1 (MQ=255)
                                                                                 tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:2504877/140‑1 (MQ=255)
                                                                                 tttCATAAACTGTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                     <  1:1912544/140‑1 (MQ=255)
                                                                                  ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                    >  1:251526/1‑140 (MQ=255)
                                                                                    cATAAACTTTCTCCTCCCAAGAGATGTGTATGTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATGCCAAGGTTTCCAATAGTCGTCAATCCGTGTGTTTGCTAGTGTTTGTCAGa                                  >  1:4934110/1‑140 (MQ=255)
                                                                                    cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgatt                                        >  1:1978320/1‑134 (MQ=255)
                                                                                    cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGa                                  >  1:2404248/1‑140 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:2422283/140‑1 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:460171/140‑1 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  1:613685/140‑1 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:337570/140‑1 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  1:2070603/140‑1 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:2354065/140‑1 (MQ=255)
                                                                                       aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                               <  2:3558237/140‑1 (MQ=255)
                                                                                         aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAGCAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                             <  2:2720302/140‑1 (MQ=255)
                                                                                         aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                             <  1:2011702/140‑1 (MQ=255)
                                                                                                cctccCAAGAGAGGATTATTTGTTTATATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGGATAACCAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                      <  2:4852398/140‑1 (MQ=255)
                                                                                                 ctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                     <  1:5188984/140‑1 (MQ=255)
                                                                                                   ccccAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                   <  2:902516/140‑1 (MQ=255)
                                                                                                   cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                   <  2:165164/140‑1 (MQ=255)
                                                                                                   cccAACAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                   <  1:3640444/140‑1 (MQ=255)
                                                                                                    ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                  <  2:1545569/140‑1 (MQ=255)
                                                                                                      aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCCGAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                <  1:3441038/140‑1 (MQ=255)
                                                                                                      aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                <  1:2979228/140‑1 (MQ=255)
                                                                                                        gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              <  1:1604141/140‑1 (MQ=255)
                                                                                                        gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              <  1:587669/140‑1 (MQ=255)
                                                                                                        gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              <  1:3603104/140‑1 (MQ=255)
                                                                                                           atgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCTTCAATCATTTTGTTTGCTATTGATTGTAAGAATTATTTATTATAGTCAGTAATg           >  1:637306/1‑140 (MQ=255)
                                                                                                           atgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           >  1:978130/1‑140 (MQ=255)
                                                                                                           atgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           <  1:3189783/140‑1 (MQ=255)
                                                                                                           atgatTATTTGTTTAAATTATATTTTTCTACGTCATTCTTCAACAAAATCCTTACCTTCATCGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           <  1:3105371/140‑1 (MQ=255)
                                                                                                           atgatTATTTGTTTAAATTATATTGTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg           <  1:1220926/140‑1 (MQ=255)
                                                                                                             gatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTa         >  2:2985041/1‑140 (MQ=255)
                                                                                                                  tttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGa    >  2:3566625/1‑140 (MQ=255)
                                                                                                                   ttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaa   >  2:4757275/1‑140 (MQ=255)
                                                                                                                   ttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaa   >  2:3878226/1‑140 (MQ=255)
                                                                                                                    tGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaaa  <  2:5075648/140‑1 (MQ=255)
                                                                                                                                atTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa              >  2:1614623/1‑116 (MQ=255)
                                                                                                                                         |                                                                                                                      
TGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAA  >  NZ_CP034943/44733‑44988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: