Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,086 G→T 6.2% S562* (TCA→TAA)  EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0860GT 6.2% 734.8 / 3.1 286S562* (TCA→TAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (66/189);  new base T (28/2);  total (94/192)
Fisher's exact test for biased strand distribution p-value = 3.55e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGT  >  NZ_CP034943/633947‑634221
                                                                                                                                           |                                                                                                                                       
ttGATAGATGTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                                                         <  1:4896862/140‑1 (MQ=255)
 tGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGa                                                                                                                                        <  1:1172734/140‑1 (MQ=255)
 tGATACATTTCCGGAACACATAGCAATTCATCTGTATGAGTAATGTTTTCTTCAATGGTTTGAACAGTGTCTTAAATTTCTTCAAGCCGACGTAGTCGTTGGCGTTATTTCTTTTTCCATTCTTTTTCTTCTTCATATGa                                                                                                                                        <  1:3777321/140‑1 (MQ=255)
       aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTGCATTATTTTTATtattaatattaaacttt                                                                                                                                  >  1:1960698/1‑135 (MQ=255)
       aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGttt                                                                                                                                  <  2:809781/140‑1 (MQ=255)
       aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTAATATGAACGttt                                                                                                                                  >  2:1866283/1‑140 (MQ=255)
        tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTTGATTGTTTTTGTTGTTGTTATGTAAGtttt                                                                                                                                 >  2:1370055/1‑140 (MQ=255)
        tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTGCAATCTTTTTATTCTTAATATGAACGtttt                                                                                                                                 >  2:3875161/1‑140 (MQ=255)
            cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                             <  1:2393174/140‑1 (MQ=255)
            cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                             <  1:722260/140‑1 (MQ=255)
            cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                             <  1:809909/140‑1 (MQ=255)
            cGGATCACATAGCAATTCATCGGTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGCCGGAGTCGTTGGCGTTCTTTCTTGTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                             <  2:3287805/140‑1 (MQ=255)
             ggATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTTGTTTTTGGGTTCTTTTTGTTGTTGATATGTAAGtttttggtg                                                                                                                            >  1:3036292/1‑135 (MQ=255)
             ggATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGGGGTTGTTTTTGCCTTATTTTTGTTGTTCATATGTACCttttttgtc                                                                                                                            >  1:176440/1‑135 (MQ=255)
                   caTAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTATTTTTGCATTATTTTTGTTGTTGAAATGGACGTTTTTATTAAGGCtt                                                                                                                      >  1:2327567/1‑140 (MQ=255)
                      aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                                                   <  2:585397/140‑1 (MQ=255)
                      aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                                                   <  2:791060/140‑1 (MQ=255)
                           ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTCCATTCTTTTTATTCTTAATATTAACGTTTTGAGTCAGGCTTAACCGAAg                                                                                                              >  2:1572807/1‑140 (MQ=255)
                           ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTGCATTGTTTTTTGTGTTGATATTTACGTTTTTTGTGAGGCTTAAACGAAg                                                                                                              >  1:1661003/1‑140 (MQ=255)
                           ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTGGTTGTTTTTGGAGTGTTTTTGTTGGTCATATTGAAGTTTTTTGTGAGGGTTatacgcag                                                                                                              >  1:2663035/1‑133 (MQ=255)
                           ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCGTCAATTTCTTCAATCCGACGGAGTCGTTTGGGGTCTTTCTGTTTACCTTCTTTTTGTTGTTCATATGGACGGGTTTCGTCAGGCTTAACCCAAg                                                                                                              >  1:39654/1‑140 (MQ=255)
                             cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTCTTTTTACATTCTTTTTCTTCTTCATATGAACGGTTTTAGTGAGGCTTAACCGAAGCa                                                                                                            >  1:929390/1‑140 (MQ=255)
                                cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTGTTTGCCCTCTTTTTGTTGTTAATATGAAACTTTTTAGTCAGGCGTAAACGAAGCAGGc                                                                                                         >  1:1196115/1‑140 (MQ=255)
                                  ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTCGTTGTTTTTGCATTATTTTTGTTATTGATATTAACGTTTTTAGTGAGGCTTTACCGAAGCCCGCGt                                                                                                       >  1:4961020/1‑140 (MQ=255)
                                  ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTTTTTTTGCAATGTTTTTGTTGATCATTATAAAGTTTTTTGTTAGGGTTTAACGAATCAGGCGt                                                                                                       >  1:3843843/1‑140 (MQ=255)
                                    aTGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTCTTTTTTGATTCATTTTTTTGTTGATATTTACGGTTTTAGTGAGGCTTAAACGAAGCAAGCCttt                                                                                                     >  1:4545905/1‑140 (MQ=255)
                                     tGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTGGAATATTTTTATTGATTAGATTAACGGTTTGTGTGAGGGATAAAAAAAACAGGCGtttt                                                                                                    >  1:4094336/1‑140 (MQ=255)
                                     tGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTTGTTTTTTGCTTGTTTTTTTTGTTGATATTGACGTTTTTTGTGAGGCTTAAAAGAAGGACGCGgttt                                                                                                    >  1:2930740/1‑140 (MQ=255)
                                      gACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGttttt                                                                                                   <  2:4700981/140‑1 (MQ=255)
                                            tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGAt                                                                                             <  2:3801319/140‑1 (MQ=255)
                                                ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTATTTTTGCAATCTTTTTATTCTTCATATGAAAGTTTTTAGTCAGGCTTAAAACAAACAGGCGTTTTTTCGGATTGct                                                                                         >  2:3348317/1‑140 (MQ=255)
                                                    tcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTACTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTg                                                                                     <  1:4689538/140‑1 (MQ=255)
                                                      aaTGTTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCATTATTAACCTAATTATTATTTTTTTCGGATTCCTCTTgat                                                                                   <  2:2703834/140‑3 (MQ=255)
                                                             tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTGCATTCTTTTTAGTCTGGATATGAAGGTTTTGAGTCAGGGTTAACCGAAGCAGGCGGTTTTTCGGCTTGCTCTTGCtgattgat                                                                            >  1:5162862/1‑140 (MQ=255)
                                                             tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGtttttt                                                                                                  >  2:4724305/1‑118 (MQ=255)
                                                                  aatGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                                                       <  1:1538314/138‑1 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGGTTGTTTTTGCATTGTTTTTGTTCTTGAAATGAAAGTTTTGAGTGAGGGTTAAACGCAGCAGGCGTTTTTTTGGATTACTGTTTGTGATTAAatttatgc                                                                     >  2:494478/1‑136 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTGTTTTTACATTATTTTTGTTCTTCATATTAACGTTTTTAGTCAGGCTTAACCGAATCAGGGGTTTTTTAGGATTACTCTTTGTGATTCAttttataa                                                                     >  2:720878/1‑136 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTACATTCTTTTTCTTCTTCATATGTAAGTTTTGAGTCAGGGTTAACCGAAGCAGGCGTTTTTTCGGGTTACTATTTGTGAGTAATTTTCTcc                                                                     >  2:4151753/1‑140 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTATTTTTCCAGTGTTTTTGGTGTTCATATGAAACTTTTTTGTCAGGCTTTACCGAAGCAGGCGTTTTTTGGGGTTGCTCTTTGTGATTCAATTTCTcc                                                                     >  2:1184871/1‑140 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTGCATTGTTTTTATTGTTCATATTAACGTTTTGAGTCAGGCTTAAACGAAGCAGGGGTTTTTTAGGATTACGCTTGGTGGTTCAttttgtgc                                                                     >  2:2515023/1‑136 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTATTTTTACATTCTTTTTGTTCTTCATATGAACGTTTTGAGTCAGGCTTAAACCAAACAGGCGTTTTTTGGGATTGCGCTTTGTGATTGAttttgtgc                                                                     >  2:3994505/1‑136 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTAGTTTTACAATCTTTTTGTTCTTCATATGAACGTTTTGAGTCAGGCTTAAACGAAGCAGGCGTTTTTTAGGATTCCTCTTTCTGATTAATTTTCAcc                                                                     >  2:3582862/1‑140 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTGGTTTTCCATTGTTTTTCTTCGTAATATGAACGTTTTGAGTCAGGCTTAAACGAAGCAGGCGGTTTTTAGGATTCCTCGTTCTGTTTAAATTTGTcc                                                                     >  2:2560975/1‑140 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGGTTGGTTTTGGGTTCTTTTTGTTGGTGATTTGAACGTTTTTAGTGAGGCTTAACCCGAGCAGGCGGTTTTTGGGGTTGCGCTTGGTGATTGAgtttgtgg                                                                     >  1:4729732/1‑136 (MQ=255)
                                                                    tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGGGTTTGTTTTTCCATTCTTTTTGTTGTTGAAATTTACGTTTTTTGTCAGGCTTAAACGAAGCAGGCGTTTTTTAGGGTTGCTCTTGGGGATTGATTTTATcc                                                                     >  1:2429530/1‑140 (MQ=255)
                                                                       cTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAAt                                                                  <  1:4058091/140‑1 (MQ=255)
                                                                            aTTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTGCATTCTTTTTCTTCTTCATATGAAAGTTTTTAGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGATTGCTATTGCGGATTGATTTTGTACCATTCAAg                                                             >  2:4195459/1‑140 (MQ=255)
                                                                                cttcAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTACCTTCTTTTTGTTGTTCATATGTACGGTTTGTGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTGCTCGTGCTGGTTGATTTTATCCAATTCAAgctgg                                                         >  2:3613274/1‑139 (MQ=255)
                                                                                 ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                        <  2:2803903/140‑1 (MQ=255)
                                                                                 ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                        <  1:3348317/140‑1 (MQ=255)
                                                                                 ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                        <  1:4527859/140‑1 (MQ=255)
                                                                                 ttcAATCCGACGGAGTCGGTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCCGGCTTAACCGAAGCAGGCGTTTGTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                        <  2:4967698/140‑1 (MQ=255)
                                                                                           cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                              <  1:3397105/140‑1 (MQ=255)
                                                                                           cGGAGTCGTTGGCGTTCTTTATTTTTCCTTTCATTTTCGTATTCATCAGACCGTTTTGAGTCAGGCTTACCCGACGCAGGCGTTTTTGCGGATTCATCTTGCTGATTCATTTTCTCCTATTCAAGCTGCTCTGttttttt                                              <  2:5115047/140‑1 (MQ=255)
                                                                                           cGGAGTCGTTGGCGTTCGTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                              <  1:4748183/140‑1 (MQ=255)
                                                                                             gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                            <  2:690979/140‑1 (MQ=255)
                                                                                              aGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtctc                                           <  2:4534451/140‑1 (MQ=255)
                                                                                                tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                         <  2:425235/140‑1 (MQ=255)
                                                                                                tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                         <  2:3217691/140‑1 (MQ=255)
                                                                                                   ttGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGtat                                      <  2:1624367/140‑1 (MQ=255)
                                                                                                    tGGAGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTCGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGtata                                     <  2:478608/140‑1 (MQ=255)
                                                                                                     ggCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa                                    <  2:1843506/140‑1 (MQ=255)
                                                                                                        gTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTAtaataa                                 <  2:2327567/140‑1 (MQ=255)
                                                                                                             ttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                            <  2:2927786/140‑1 (MQ=255)
                                                                                                             ttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTATTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                            <  2:129999/140‑1 (MQ=255)
                                                                                                               ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCTTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                          <  2:5215242/140‑1 (MQ=255)
                                                                                                               ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                          <  2:3672214/140‑1 (MQ=255)
                                                                                                               ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                          <  2:3378230/140‑1 (MQ=255)
                                                                                                               ctttTTCCATTCTTTTTCGTCTTCATATGAACGTTTTGAGTCAGGCTTAAGCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                          <  2:906415/140‑1 (MQ=255)
                                                                                                               cttcgtCCATGCTATCTCTTCTCCCTATGACCGTTTAGAGTCAGGCGGACCCGAAGCAGCCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAAGTCAAGCTGCTCTGTTTTTTTCTGCGTATAATAATCGTAAt                          <  2:3544496/135‑1 (MQ=255)
                                                                                                                      cATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAg                   >  2:3400366/1‑140 (MQ=255)
                                                                                                                       aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa                  <  2:176440/140‑1 (MQ=255)
                                                                                                                       aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa                  <  2:4238332/140‑1 (MQ=255)
                                                                                                                       aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa                  <  2:3036292/140‑1 (MQ=255)
                                                                                                                       aTTCTTTTTATTATTCATATCATCGTTTTGAGTCAGGCTTAACCTAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATATTCGTAATCTCCCAGa                  <  2:2055939/140‑1 (MQ=255)
                                                                                                                        ttCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGAt                 >  2:103073/1‑140 (MQ=255)
                                                                                                                        ttCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTATCCGTATAATAATCGTAATATCCCAGAt                 >  1:3139168/1‑140 (MQ=255)
                                                                                                                         tCTTTTTCTTCTTCATATTAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                <  1:1370800/140‑1 (MQ=255)
                                                                                                                         tCTTTTTCTTCTTCATATGCACGTTTTGAGTCAGGCTTACCCGCAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                <  2:2091499/140‑1 (MQ=255)
                                                                                                                         tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                <  1:3327538/140‑1 (MQ=255)
                                                                                                                         tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                <  2:4494132/140‑1 (MQ=255)
                                                                                                                         tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTCTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                <  2:2215518/140‑1 (MQ=255)
                                                                                                                         tCTTTTTCTTCTTCAGATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                <  1:2516281/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  2:1263959/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  1:3049144/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  2:3322764/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  2:3186614/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  2:2426653/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  2:3714625/140‑1 (MQ=255)
                                                                                                                           tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCAGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct              <  1:1059922/140‑1 (MQ=255)
                                                                                                                             tttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa                                    <  2:1093683/116‑1 (MQ=255)
                                                                                                                                 ttcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCta        >  1:4238976/1‑140 (MQ=255)
                                                                                                                                  tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat       <  1:2730231/140‑1 (MQ=255)
                                                                                                                                  tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat       <  1:1816429/140‑1 (MQ=255)
                                                                                                                                  tcttcATATGAACGTTTTGAGTCAGGATTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCACAGATACTCTTCtat       <  1:4204191/140‑1 (MQ=255)
                                                                                                                                   cttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtata      <  2:4899657/140‑1 (MQ=255)
                                                                                                                                     tcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTATTCTATATg    >  2:4434296/1‑140 (MQ=255)
                                                                                                                                     tcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATAGTAATATCCCAGATACTCTTCTATATg    >  1:2816988/1‑140 (MQ=255)
                                                                                                                                     tcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATAGTAATATCCCAGATACTCTGCTAtgtg    >  1:3598784/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGT  >  NZ_CP034943/633947‑634221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: