BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	814,356	T→G	8.8%	H340Q (CAT→CAG)	nosA →	nitric oxide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	814,356	0	T	G	8.8%	1020.5 / 22.3	386	H340Q (CAT→CAG)	nosA	nitric oxide synthase
Reads supporting (aligned to +/- strand): ref base T (124/213); new base G (46/3); total (170/216)
Fisher's exact test for biased strand distribution p-value = 3.99e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

AGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCTT  >  NZ_CP034943/814217‑814493

   aaGGTGTCAGCATCGGGGACCCTCAGACGGCCGCAAGCCAGTTTAAACGGTTTGACGAACAGGAGGAAGCAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCCCTCATATc                                                                                                                                        <  2:4959644/140‑1 (MQ=255)

        gtCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCa                                                                                                                                   <  2:259564/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                 <  2:4551589/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                 <  2:4216970/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                 <  1:1341372/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                 <  1:1556623/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                 <  2:2666039/140‑1 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCt                                                                                                                           >  2:142993/1‑140 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCt                                                                                                                           >  1:3087361/1‑140 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCt                                                                                                                           >  1:1369196/1‑140 (MQ=255)

                 gTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTa                                                                                                                          >  1:91244/1‑140 (MQ=255)

                 gTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTa                                                                                                                          >  1:1862054/1‑140 (MQ=255)

                 gTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTa                                                                                                                          >  2:2278982/1‑140 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTAt                                                                                                                         >  1:1958906/1‑140 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTAt                                                                                                                         >  2:798654/1‑140 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTAt                                                                                                                         <  2:2371434/140‑1 (MQ=255)

                    gACCATCATACGGCCGCAAGCCAGTTTAAACGTTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGa                                                                                                                       <  1:2655890/140‑1 (MQ=255)

                       catcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACaa                                                                                                                    >  2:4084585/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCTGATCTTCCACCGTTCCTATGACAACt                                                                                                                  >  2:2316578/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                  >  2:4429063/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                  >  2:279981/1‑140 (MQ=255)

                          catACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTc                                                                                                                 >  1:3141938/1‑140 (MQ=255)

                               ggCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCg                                                                                                            >  2:917131/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                          >  2:1485208/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACACCTCAATCGtt                                                                                                          >  1:5172935/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                          >  1:4111012/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                          >  1:818419/1‑140 (MQ=255)

                                   gCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATc                                                                                                                                        <  1:4803237/108‑1 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       >  1:948230/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       <  2:5077291/140‑1 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       >  2:2735448/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       >  2:706698/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       <  2:1836527/140‑1 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       <  2:2499798/140‑1 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTACACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                       >  1:454268/1‑140 (MQ=255)

                                           gTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAAtt                                                                                                >  1:2860209/1‑140 (MQ=255)

                                            tttAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTa                                                                                               >  2:1275442/1‑140 (MQ=255)

                                              tAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACt                                                                                             <  2:1898910/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAACGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                        <  2:3569456/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                        <  1:1505738/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                        <  1:2306946/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGAGTCCGCCGATATCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACACCTCAATCGTTAAGCCGAATTACTTGTAt                                                                                        <  1:2958916/140‑1 (MQ=255)

                                                        gaagaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGa                                                                                   <  2:2418423/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                 <  2:1145289/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                 <  1:1164599/140‑1 (MQ=255)

                                                               atgaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            <  2:315319/138‑1 (MQ=255)

                                                               agtaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            <  1:1589971/137‑1 (MQ=255)

                                                               aggaggAAGCAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            <  2:1760740/140‑1 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            <  1:100824/140‑1 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            <  1:3468745/140‑1 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            <  2:1462083/140‑1 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                            >  1:4775783/1‑140 (MQ=255)

                                                                 gaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTa                                                                          <  1:4858425/140‑1 (MQ=255)

                                                                      agaagCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGt                                                                     >  1:3272242/1‑140 (MQ=255)

                                                                           cGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaaca                                                                >  1:4330081/1‑140 (MQ=255)

                                                                           cGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaaca                                                                >  2:3880098/1‑140 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGaa                                                                                                  >  2:1353285/1‑104 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTACCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaacaac                                                              >  1:570058/1‑140 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGACTTACTTTTATCAGGATAAGCCTTACGAGTAaacaac                                                              >  1:2509536/1‑140 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaacaac                                                              >  1:3650545/1‑140 (MQ=255)

                                                                                          cGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTCTGACAACTCACTCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGa                                                 <  2:3087361/140‑1 (MQ=255)

                                                                                          cGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGa                                                 <  2:1510512/140‑1 (MQ=255)

                                                                                          cGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGa                                                 <  2:1020105/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGCTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                               <  2:3672151/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                               <  2:5019091/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                               <  1:3551206/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGAGTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                               <  2:3472618/140‑1 (MQ=255)

                                                                                                aCTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTaaa                                                                  <  2:2219679/117‑1 (MQ=255)

                                                                                                 cTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCtt                                          <  2:2876475/140‑1 (MQ=255)

                                                                                                  tGGACGTGGCTGAGTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTc                                         <  2:643444/140‑1 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGGTGCCACTCAGCTCTTCCACCGTTCCTATGACCACTCAATCGTTAAGCCGACTTACTTTTATCCGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCa                                        >  1:1715902/1‑140 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCa                                        >  1:2158809/1‑140 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                  <  2:2860209/140‑1 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                  <  2:41998/140‑1 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                  >  2:3319193/1‑140 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                  >  1:3220476/1‑140 (MQ=255)

                                                                                                              agtCCGCCGATTTCACCCGCTGCCACTCATATCTTCCCCCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTGTTATCAGGATAAGCCTTACGAGTAAACAACGGAAACTCGGTGATGGCCTTCAAGGCTGCGGGa                             <  2:5172935/138‑1 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGATAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAACTCGGTGATGGCCTTCAAGGCTGCGGGa                             >  2:4662249/1‑140 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGa                             >  1:1224741/1‑140 (MQ=255)

                                                                                                                tCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGAtt                           <  2:4770938/140‑1 (MQ=255)

                                                                                                                    ccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtgt                       >  1:1061422/1‑140 (MQ=255)

                                                                                                                    ccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtgt                       >  1:4381601/1‑140 (MQ=255)

                                                                                                                    ccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCATTACGAGTAAACAACGGAAAAACGGTGAAGGCCTTCAAGGCTGCGGGATTCtgt                       >  1:4981038/1‑140 (MQ=255)

                                                                                                                        tttCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACa                   >  2:1633944/1‑140 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  1:3921588/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  2:1596348/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  1:4226996/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  1:3738518/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  2:2958951/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATATCTTCCACCGTTACTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACCACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  2:3925794/140‑1 (MQ=255)

                                                                                                                            aCCCGCTGCCACTCATAACGTCCACCGTTCCTATGACCACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAgaga               <  1:3385633/140‑1 (MQ=255)

                                                                                                                                  tGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCgg         <  2:4723365/140‑1 (MQ=255)

                                                                                                                                  tGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCgg         <  2:4852899/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt        <  1:2346143/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt        <  1:3831862/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt        <  1:3213067/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt        <  1:5086566/140‑1 (MQ=255)

                                                                                                                                    ccACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTa       <  1:2253872/140‑1 (MQ=255)

                                                                                                                                    ccACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTa       <  2:2003413/140‑1 (MQ=255)

                                                                                                                                      aCTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAAc     >  2:3348762/1‑140 (MQ=255)

AGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCTT  >  NZ_CP034943/814217‑814493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`