Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 886,747 A→C 10.5% P47P (CCT→CCG)  EO946_RS04490 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943886,7470AC10.5% 505.0 / 2.5 232P47P (CCT→CCG) EO946_RS04490MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (128/60);  new base C (2/36);  total (130/102)
Fisher's exact test for biased strand distribution p-value = 1.36e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACA  >  NZ_CP034943/886613‑886886
                                                                                                                                       |                                                                                                                                           
      aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGCCAGCTTTCCGACGATAGGCACACTGAAGCTAAGGCCGAGCGGATAGAGGGTTATGCCCCCTG‑ACCCCCCGGAAGGAGAAACGGc                                                                                                                                  >  1:2507824/1‑140 (MQ=255)
      aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGCAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGACCCATG‑ACCCCCAGGAAGGAGaaaacgc                                                                                                                                  >  1:591241/1‑136 (MQ=255)
      aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCCGGAAGGAGAAACGGc                                                                                                                                  >  1:5188000/1‑140 (MQ=255)
      aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGGAGAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGc                                                                                                                                  >  2:2635000/1‑140 (MQ=255)
       gTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGTCGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCa                                                                                                                                 >  2:1471869/1‑140 (MQ=255)
       gTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCa                                                                                                                                 >  2:3723072/1‑140 (MQ=255)
           gAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAATGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCCGCACGGGGAAACGGCAAAcc                                                                                                                             >  1:2708738/1‑139 (MQ=255)
                 gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGAGCGGCACACTGACGGTAAGGCCGAGGGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAAGGACTCGt                                                                                                                       >  1:1679314/1‑140 (MQ=255)
                  cAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCCGGAAGGAGAAACGGCAAAAGACACGtt                                                                                                                      >  1:2617919/1‑140 (MQ=255)
                      ccTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACGGACGCTAAGGCCGAGCGTCTAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGGTTATc                                                                                                                  >  1:907111/1‑140 (MQ=255)
                           aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                             >  2:4373872/1‑140 (MQ=255)
                           aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                             >  2:1448014/1‑140 (MQ=255)
                           aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                             >  1:285573/1‑140 (MQ=255)
                                    cttttctttcTGCCGTAGGGGTCGGAAAGGTTTCCGAGGTTCGCCTCACTGAGGCTCCGCCCGTGGGTATCGAGGGTACTGCCCCATC‑ACCCCCACGACGGAGAAAGGGACAACGACTCGTTTATCGTTGTTAAagcagc                                                                                                    <  2:3475398/138‑1 (MQ=255)
                                                ccGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTcc                                                                                        >  2:2976340/1‑140 (MQ=255)
                                                        gTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCACGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCa                                                                                >  1:3444716/1‑140 (MQ=255)
                                                           tgAATGCTTTCCGACTATGGGCACACTGCCGCTAAGGCGGAGCTTATAGTGGTTAATGCCCCATC‑ACCCCCCGTACGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:591241/139‑1 (MQ=255)
                                                           gtacaggtttCGGACTATGGGCACACTGAGGCTACGGCCGTGCGTATTGCGGGTAATGCCCACTG‑ACCCCAAGTACGGTGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:4534555/133‑1 (MQ=255)
                                                           gtaaaGGTTCCCGACGATCGGAAAACGGACGCTACGGCCGCGCGTATAGAGGGTAATGCCCCATG‑CCCCCCAGCAACGACAACCGGCACACGACGCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:545506/138‑1 (MQ=255)
                                                           gtaaaGCTTACCGACGATCGGCACCCTGACGCTAAGGCCGTGCTTATAGAGGGTAATGCCCCAGG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:5188000/138‑1 (MQ=255)
                                                           gcaacgCTTTCCGCCGCTCGGCACCCTCACGCTCAGCCCTAGCGAACAGCGGGTAAGGCCCCATC‑ACCCCCCTGACGCAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCCGCAGAAATAAGTCCGTTATCCAgcg                                                                             <  2:2305821/135‑1 (MQ=255)
                                                                   ttCCGACGATGGGAACACTGCCGCTACGGCCGAGCGTATAGCGGTTAATGCCCAATG‑ACCCCCATCAAGGATACACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATa                                                                     <  2:1425815/140‑1 (MQ=255)
                                                                   taccGACGAGCGGCACACTGACGCTAAGGCCGAGGGTATAGAGGGTAATGCCCCTTG‑ACCCCCAGGAAGGAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATa                                                                     <  2:3520313/138‑1 (MQ=255)
                                                                     cccactaTCGGAACACTGACGATAAGGCCGAGCTTATCGTGGTTAATGCCCATTG‑ACCCCCCGGATGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                   <  1:954841/134‑1 (MQ=255)
                                                                          taTCGGCACACTTACGCTACGGCCGCGCTTATAGAGGTTAATGCCCCATG‑ACCCCCAGGACGGAGAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:5130539/139‑1 (MQ=255)
                                                                          gttCGGCACCCTGACGCTACGGCCGAGCTTATATAGGGTAATGCCCAATG‑ACCCCCAGGAAGGACAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  2:249783/138‑1 (MQ=255)
                                                                          gaTGGGCACACTGACGCTATGGCCGAGCTTATTGAGTTTAAGGCCCATTC‑ACCCCCAGTATGTTTAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:3116949/140‑1 (MQ=255)
                                                                          gaTGGGAACACTGACGCTAAGGCCGAGCTTATAGTGGGTAATGCCCCATC‑ACCCCCAGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:3608162/140‑1 (MQ=255)
                                                                          gaTCGGCACACTGACGCTACGGCCGCGCGTTTAGTGGTTAATCCCCAATC‑ACCCCCAGTACGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:1448014/140‑1 (MQ=255)
                                                                          gaTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              >  1:4695431/1‑140 (MQ=255)
                                                                          gaTCGGCACACTGACGCTAAGGCCGAGCGATTTGAGTGTATGGCCCCATC‑ACCCCCTGGAAGTATAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:3226170/140‑1 (MQ=255)
                                                                          gaTCGGCACACTCCCCCAACGGCCGAGCTTATACAGGTTAAGGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:4028227/140‑1 (MQ=255)
                                                                          gaTCGGAACACTCTCGGTACGGCTTAGTGTATAGAGGTTAATGCCCCATG‑ACCCCAAGGATGTATAAACGGAAAACGACTCGTTTATAGTTGTTAAATCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAaaacac                                                              <  2:3703960/140‑1 (MQ=255)
                                                                          gaTCGCAACACTTCCGCTACGGCGGTGGGTATAGTGGTTAATG‑CCCATGCACCCCACCTAAGCAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  2:509794/140‑1 (MQ=255)
                                                                              tgaaaaatGACGATAAGGCCTAGGTTTTAGTGGTTATTGCCCCATG‑ACCCCCTGTATGTATAAAGGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                          <  2:691609/133‑1 (MQ=255)
                                                                              ggCACAGTGACGCTAAGGCCGAGCGTATAGAGGTTAATGCCCCATG‑ACCCCCTGGACGGAGAAAGGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                          <  2:4454920/140‑1 (MQ=255)
                                                                              ggCACACTGACGATACGGCCGAGCTTATAGTGGGTAATGCCCAATG‑ACCCCCAGGAAGTAGAAACGTCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                          <  2:1767963/140‑1 (MQ=255)
                                                                              gacacaCTCACGATAAGCCCCAGCTTATGGCGGGTAAGCCCCCTTG‑ACCCCCCGTCCGGATACCCGCAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                          <  1:311735/138‑1 (MQ=255)
                                                                                  cacTGCCGCTTCGGCCGAGCGTATTGTGGGTATGCCCCCCTG‑ACCCCCAGGAAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTa                                                      <  1:3691739/140‑1 (MQ=255)
                                                                                  cacTGACGCTACGGCCGAGCGTATAGCGGTTAATCCCCCATG‑ACCCCCCGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTa                                                      <  1:4753711/140‑1 (MQ=255)
                                                                                  cacTGACGCAACGCCCTCGCTTATTGAGGTTACTGCCCCATG‑ACCCCCAGGAAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTa                                                      <  1:1145478/140‑1 (MQ=255)
                                                                                    cTGAGGCTAAGGCGGAGCTTATTGCGGTTAATGCCCCATC‑ACCCCAAGGACGTATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                    <  1:4373872/140‑1 (MQ=255)
                                                                                       aCGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAg                                                 >  1:1895361/1‑140 (MQ=255)
                                                                                          cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                              >  2:4705925/1‑140 (MQ=255)
                                                                                            ccggCCGAGCGTATAGAGGGTAAGGCCCCATC‑ACCCCCCCGACGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGa                                            <  1:1591585/138‑1 (MQ=255)
                                                                                                 accgCGTAGAGAGGGTCAGGCCCAAGG‑ACCCCCAGCCCGGACAACCGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                       <  2:2678499/137‑1 (MQ=255)
                                                                                                   aGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                     >  2:2864595/1‑140 (MQ=255)
                                                                                                   aGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                     >  2:1406516/1‑140 (MQ=255)
                                                                                                   aGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                     >  2:1310730/1‑140 (MQ=255)
                                                                                                   aGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGAAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAAAGTAAACAGGCTGATCGCCCa                                     >  2:2030017/1‑140 (MQ=255)
                                                                                                         taGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                               >  2:1128380/1‑140 (MQ=255)
                                                                                                         taGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCAGAAACACACCAATCGTAAACAGGCTGATTGCCCAcggttg                               >  2:1553736/1‑135 (MQ=255)
                                                                                                          caggggTAATGCCCCATG‑ACCCCAACCACGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                              <  2:5047271/137‑1 (MQ=255)
                                                                                                          caggggTAAGGCCCCATG‑ACCCCCAGCACGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                              <  2:3824982/137‑1 (MQ=255)
                                                                                                          agtgtttAATGCCCCATG‑ACCCCCAGCAAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                              <  1:4558681/134‑1 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGAc                                                                                                                           <  2:4139891/43‑1 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCCATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:3315153/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:1025981/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:4307924/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:4849648/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:4651401/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:4850160/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:5127314/1‑140 (MQ=255)
                                                                                                              ggTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGAAAACATGCTTATCGACCACTTTTGTCttt                          >  2:4317329/1‑140 (MQ=255)
                                                                                                               gTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                         <  2:1895361/140‑1 (MQ=255)
                                                                                                                 aaTGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  1:2628529/1‑140 (MQ=255)
                                                                                                                 aaTGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  1:4245981/1‑140 (MQ=255)
                                                                                                                 aaTGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  1:2341867/1‑140 (MQ=255)
                                                                                                                 aaTGCCCCATG‑ACCAACAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGTCTGATCGCCCACTTTTGGCTTTTCt                       >  1:2034544/1‑140 (MQ=255)
                                                                                                                  atcccccAGC‑ACCCCCAGGAAGTACAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAACTACTTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACATTTGGCTTTTCTg                      <  2:3995461/137‑1 (MQ=255)
                                                                                                                  aTGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                      >  1:4122677/1‑140 (MQ=255)
                                                                                                                  aTGCCCCATG‑ACCCCCAGCAAGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGTGATCCAGCGCAGCCATAAACACACCAATCGTACACAGGCTGATCGCCCACTTTTGGCTTTTCTg                      <  2:1824987/140‑1 (MQ=255)
                                                                                                                   tGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  1:2177822/84‑1 (MQ=255)
                                                                                                                   tGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                     >  1:78580/1‑140 (MQ=255)
                                                                                                                    gCCCCATG‑ACCCCCCGGAAGCAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  1:5030831/140‑1 (MQ=255)
                                                                                                                    cccccATG‑ACCCCCAGGACGGACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  2:4198204/139‑1 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATACACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  1:389576/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGctctct                >  1:4922833/1‑138 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  2:3326249/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  1:4875797/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  2:2021592/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  1:422789/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  2:2776431/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  1:3812036/1‑140 (MQ=255)
                                                                                                                        cATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTATGt                >  2:3751543/1‑140 (MQ=255)
                                                                                                                         aTG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTc               >  1:1174930/1‑140 (MQ=255)
                                                                                                                          tG‑ACCCCCAGGACGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              <  2:5008359/140‑1 (MQ=255)
                                                                                                                          tG‑ACCCCCAGGACGCAGAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              <  2:2114920/140‑1 (MQ=255)
                                                                                                                          tG‑ACCCCCAGGAAGTATAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              <  2:3181470/140‑1 (MQ=255)
                                                                                                                           g‑ACCCCCCGTAAGGACAACCGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa             <  2:4883307/140‑1 (MQ=255)
                                                                                                                           g‑ACCCCCCGGACGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa             <  2:2207544/140‑1 (MQ=255)
                                                                                                                                 cctgcaagtaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt        <  1:3741137/135‑1 (MQ=255)
                                                                                                                                 cccgcaaggaGAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt        <  2:3444716/135‑1 (MQ=255)
                                                                                                                                 ccAGTAAGCACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCCATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt        <  1:36369/140‑1 (MQ=255)
                                                                                                                                 ccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt        <  2:4060998/140‑1 (MQ=255)
                                                                                                                                   aggaaggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCa      >  1:2570678/1‑140 (MQ=255)
                                                                                                                                       aggaTAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa  <  2:3594779/140‑1 (MQ=255)
                                                                                                                                       aggaGACACCGCACTCGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa  <  1:2976340/140‑1 (MQ=255)
                                                                                                                                       aggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa  <  2:907111/140‑1 (MQ=255)
                                                                                                                                       aggaAAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCACCAGAAATAATTCCGTTATCCAGCGCAGCCATAACCACACCACTCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa  <  1:2260811/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
GAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATG‑ACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACA  >  NZ_CP034943/886613‑886886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑