Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 924,135 A→T 5.4% intergenic (+10/+41) EO946_RS04760 → / ← spo0M tRNA‑Leu/sporulation control protein Spo0M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943924,1350AT 5.4% 918.1 / 4.6 298intergenic (+10/+41)EO946_RS04760/spo0MtRNA‑Leu/sporulation control protein Spo0M
Reads supporting (aligned to +/- strand):  ref base A (117/160);  new base T (19/2);  total (136/162)
Fisher's exact test for biased strand distribution p-value = 1.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

ATCCATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAGCTGTT  >  NZ_CP034943/923998‑924273
                                                                                                                                         |                                                                                                                                          
aTCCATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGc                                                                                                                                          >  2:4762937/1‑140 (MQ=255)
aTCCATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGc                                                                                                                                          >  2:3367168/1‑140 (MQ=255)
aTCCATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGc                                                                                                                                          >  2:222145/1‑140 (MQ=255)
  ccATGTTATATTTAGATTCGTCGGTTAGATACGACGTAAAACTTGCCGGGGTGGCGGAATTGGCAGACGCGCACGACTCAAAAGCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGAGCACCGGGATCCTGACATAGccg                                                                                                                                        >  1:3824556/1‑139 (MQ=255)
  ccATGATCTATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAATGccc                                                                                                                                        >  2:1490164/1‑140 (MQ=255)
  ccATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAATGccc                                                                                                                                        >  1:1492021/1‑140 (MQ=255)
  ccATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGccc                                                                                                                                        >  1:4958008/1‑140 (MQ=255)
  ccATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGccc                                                                                                                                        <  2:1619653/140‑1 (MQ=255)
  ccATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGccc                                                                                                                                        <  2:4958008/140‑1 (MQ=255)
  ccATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGccc                                                                                                                                        <  1:2412061/140‑1 (MQ=255)
  ccATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGccc                                                                                                                                        >  1:3505354/1‑140 (MQ=255)
       atatatTTATATTCGTCGGTTCGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTc                                                                                                                                   <  1:5002552/140‑1 (MQ=255)
        tatatttatattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCt                                                                                                                                  <  1:1013229/140‑1 (MQ=255)
           atttatattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTtat                                                                                                                               >  1:2210265/1‑140 (MQ=255)
           atttatattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTtat                                                                                                                               >  1:2063109/1‑140 (MQ=255)
           atttatattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTtat                                                                                                                               >  1:2743730/1‑140 (MQ=255)
           atttatattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTtat                                                                                                                               >  2:3865389/1‑140 (MQ=255)
               atattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACat                                                                                                                           >  2:62164/1‑139 (MQ=255)
               atattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAg                                                                                                                           >  2:2183693/1‑140 (MQ=255)
               atattCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGGATACTGAAAAAGCCCGTTTCTTATACat                                                                                                                           >  2:2645885/1‑139 (MQ=255)
                 attCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGc                                                                                                                                          <  1:2638151/123‑1 (MQ=255)
                  ttCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACAACCGGTATACTGAAAAAGCCCGTTTCTTATACAGaaa                                                                                                                        >  1:4103656/1‑140 (MQ=255)
                    cgtcgGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAATGCCCGTTTCTTATACAGAAACg                                                                                                                      >  2:3120234/1‑140 (MQ=255)
                    cgtcgGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACg                                                                                                                      >  2:2821270/1‑140 (MQ=255)
                      tcgGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACggg                                                                                                                    >  2:5184150/1‑140 (MQ=255)
                      tcgGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACggg                                                                                                                    >  1:3936749/1‑140 (MQ=255)
                        ggTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGtt                                                                                                                  <  2:1801941/140‑1 (MQ=255)
                                  gacgTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAATGCCCGTTTCTTATACAGAAACGGGtttttattttta                                                                                                        >  2:4020749/1‑140 (MQ=255)
                                   acgTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTttttatttttat                                                                                                       >  1:2330218/1‑140 (MQ=255)
                                   acgTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTttttatttttat                                                                                                       <  2:2063109/140‑1 (MQ=255)
                                   acgTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTgtttattgttat                                                                                                       >  1:4768581/1‑140 (MQ=255)
                                        aaaCTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTc                                                                                                  <  2:776561/140‑1 (MQ=255)
                                         aaCTTGCCGGTGTGGCGGAATGGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCa                                                                                                 <  2:1094353/140‑1 (MQ=255)
                                           ctggCCGGGGTGGCGGAATTGGCAGACCCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATAATGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACt                                                                                               <  2:3934140/137‑1 (MQ=255)
                                           cTTGCCGGTGTGGCGTAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACt                                                                                               <  1:4031514/140‑1 (MQ=255)
                                           cTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACt                                                                                               <  2:132888/140‑1 (MQ=255)
                                           cTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACt                                                                                               <  2:328532/140‑1 (MQ=255)
                                           cTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGGTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACt                                                                                               <  2:4687287/140‑1 (MQ=255)
                                             tGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCa                                                                                             <  1:4240218/140‑1 (MQ=255)
                                              gCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAATGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAg                                                                                            >  2:2466052/1‑140 (MQ=255)
                                              gCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACATAAACGGGTTTTTATTTTTATAATTCACTCAg                                                                                            >  1:184118/1‑140 (MQ=255)
                                              gCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAg                                                                                            >  1:4329963/1‑140 (MQ=255)
                                              gCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAg                                                                                            >  1:708908/1‑140 (MQ=255)
                                              gCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAg                                                                                            >  1:2172246/1‑140 (MQ=255)
                                              gCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAg                                                                                            >  1:186213/1‑140 (MQ=255)
                                                  gtgtGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTa                                                                                        <  1:2821270/140‑1 (MQ=255)
                                                  gtgtGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTa                                                                                        <  1:3120234/140‑1 (MQ=255)
                                                  gtgtGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAAGTCACTCAGCGTa                                                                                        <  1:5184150/140‑1 (MQ=255)
                                                     tgGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTg                                                                                     <  2:129413/140‑1 (MQ=255)
                                                        cGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTc                                                                                  >  1:744001/1‑140 (MQ=255)
                                                         ggAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCt                                                                                 <  2:2407878/140‑1 (MQ=255)
                                                             ttGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCATCGTATTGGTCTATGa                                                                             >  2:438073/1‑140 (MQ=255)
                                                             ttGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGAACACAGGTATACTGAAAAAGCCCGTTTCTTATACAGAAAAGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGa                                                                             <  1:1287800/140‑1 (MQ=255)
                                                              tGGCAGACGCTCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAACAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAt                                                                            <  2:3202571/140‑1 (MQ=255)
                                                              tGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAt                                                                            <  2:4440478/140‑1 (MQ=255)
                                                              tGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAt                                                                            <  2:1126642/140‑1 (MQ=255)
                                                               ggCAGACGCGCACGACTCAAAATCGTGTTCCTTGTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAtc                                                                           <  2:1502502/140‑1 (MQ=255)
                                                               ggCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAtc                                                                           <  2:869013/140‑1 (MQ=255)
                                                                gCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAtct                                                                          <  1:3865367/140‑1 (MQ=255)
                                                                 cAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGAtctc                                                                         <  2:3756248/140‑1 (MQ=255)
                                                                     cgcgCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCtcttct                                                                     >  1:647180/1‑140 (MQ=255)
                                                                         cACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTa                                                                 <  2:3482384/140‑1 (MQ=255)
                                                                         cACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTa                                                                 <  1:2229054/140‑1 (MQ=255)
                                                                         cACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTa                                                                 <  1:1435684/140‑1 (MQ=255)
                                                                          aCGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTAc                                                                <  1:4057552/140‑1 (MQ=255)
                                                                                 aaaaTCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTa                                                         <  1:3367168/140‑1 (MQ=255)
                                                                                 aaaaTCGTGTTCCTTATGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTa                                                         <  1:2989374/140‑1 (MQ=255)
                                                                                    aTCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGc                                                      <  1:3270405/140‑1 (MQ=255)
                                                                                        tgtTCCTTCTGTATTGTCGGTTCGACCCAGACCACAGGTATACTGAAAAAGCCCGTTTATTATAAAGAAAGGGGTTTTTATTTTTATAATTCACTCATATTATTTGTCTATCATATCTTCTATTACCTCTTTAAGCtctt                                                  >  2:4277002/1‑140 (MQ=255)
                                                                                        tgtTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTc                                                                                                                                   <  1:808899/59‑1 (MQ=255)
                                                                                        tgtTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCtctt                                                  >  1:1169835/1‑140 (MQ=255)
                                                                                        tgtTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTAGACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGAATTGGTCTATGATCTCTTCTAGTACCTCTATAAGctggt                                                  >  2:5142109/1‑137 (MQ=255)
                                                                                           tCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTg                                               <  1:914061/140‑1 (MQ=255)
                                                                                           tCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTg                                               <  1:3409425/140‑1 (MQ=255)
                                                                                            ccTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGc                                              <  2:1392731/140‑1 (MQ=255)
                                                                                             cTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCa                                             <  2:1051285/140‑1 (MQ=255)
                                                                                             cTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCa                                             <  2:4153036/140‑1 (MQ=255)
                                                                                               tCTGGAGTGTCGGTTCGAACCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGCTCTCGTCTAGTACCTCTTTAAGCTCTTCTGCCTc                                           >  2:4888288/1‑140 (MQ=255)
                                                                                                     gtgtCGGTTCGACCCCGACCAGCGGTATCCCGAAAAAGCCCGTTTCTTATACAGAACCGGGTTTTTATTTGTATAAGTCACTCAGCGTATTGGTCTCTGAGCTCTTCTCGTACCACTTTAAGCTCTGCTGCCTCTTGAAg                                     <  2:3405569/140‑1 (MQ=255)
                                                                                                     gtgtCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAg                                     <  1:1572656/140‑1 (MQ=255)
                                                                                                     gtgtCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAg                                     <  1:339297/140‑1 (MQ=255)
                                                                                                     gtgtCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAg                                     <  1:3774718/140‑1 (MQ=255)
                                                                                                     gtgtCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAg                                     <  1:4495756/140‑1 (MQ=255)
                                                                                                     gggtCGGTTCGACCCCGACCACCGGTATACAGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAg                                     <  1:2685636/138‑1 (MQ=255)
                                                                                                      tgtCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGc                                    >  2:4132119/1‑140 (MQ=255)
                                                                                                        tCGGTTCGACCCCGACCACCGGTATACTGAAAATGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCACGctc                                  >  1:1084906/1‑140 (MQ=255)
                                                                                                        tCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGctc                                  >  2:2526637/1‑140 (MQ=255)
                                                                                                        tCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGct                                   <  1:4719501/139‑1 (MQ=255)
                                                                                                        tCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGct                                   >  2:4719501/1‑139 (MQ=255)
                                                                                                                aCCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTAtt                                                                                      >  1:5120259/1‑80 (MQ=255)
                                                                                                                aCCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTAtt                                                                                      <  2:5120259/80‑1 (MQ=255)
                                                                                                                aCCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGc                          <  2:1849030/140‑1 (MQ=255)
                                                                                                                           cGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGaca               >  2:3110199/1‑140 (MQ=255)
                                                                                                                                taCTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTAc          <  2:300885/140‑1 (MQ=255)
                                                                                                                                   tGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAg       <  1:2491920/140‑1 (MQ=255)
                                                                                                                                     aaaaaGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAGCt     >  1:319048/1‑140 (MQ=255)
                                                                                                                                     aaaaaGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAGCt     >  1:2892751/1‑140 (MQ=255)
                                                                                                                                      aaaaGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAGCTg    >  2:377780/1‑140 (MQ=255)
                                                                                                                                       aaaGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAg       <  1:4568006/136‑1 (MQ=255)
                                                                                                                                       aaaGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAg       >  2:4568006/1‑136 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ATCCATGATATATTTATATTCGTCGGTTAGATACGACGTAAAACTTGCCGGTGTGGCGGAATTGGCAGACGCGCACGACTCAAAATCGTGTTCCTTCTGGAGTGTCGGTTCGACCCCGACCACCGGTATACTGAAAAAGCCCGTTTCTTATACAGAAACGGGTTTTTATTTTTATAATTCACTCAGCGTATTGGTCTATGATCTCTTCTAGTACCTCTTTAAGCTCTTCTGCATCTTCAAGCTCTGATTGGCTGAAGCGGACACGTACGAGCTGTT  >  NZ_CP034943/923998‑924273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: