Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 986,801 G→T 10.3% D372Y (GAT→TAT)  spoVR → stage V sporulation protein SpoVR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943986,8010GT10.3% 657.4 / 14.1 283D372Y (GAT→TAT) spoVRstage V sporulation protein SpoVR
Reads supporting (aligned to +/- strand):  ref base G (114/125);  new base T (2/39);  total (116/167)
Fisher's exact test for biased strand distribution p-value = 2.54e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCATTGGT  >  NZ_CP034943/986664‑986939
                                                                                                                                         |                                                                                                                                          
gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                                          >  2:3717129/1‑140 (MQ=255)
gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                                          >  2:3872498/1‑140 (MQ=255)
   tGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATtat                                                                                                                                       >  1:4896869/1‑140 (MQ=255)
     aaGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATaa                                                                                                                                     >  2:2136246/1‑140 (MQ=255)
     aaGCCTGGCTCAGGCAGGGAGAAAATGTGTGACGTCCGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATTCGCGAGGATCTTTATTATTTTCAAAAAAAGGGATGAGAtaataa                                                                                                                                     >  2:1650894/1‑140 (MQ=255)
          tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                                >  2:2412014/1‑140 (MQ=255)
          tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAAGTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                                >  2:1971067/1‑140 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                          <  2:4511647/140‑1 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                        >  1:1960663/1‑140 (MQ=255)
                   caggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAg                                                                                                                       <  2:3423263/140‑1 (MQ=255)
                   caggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAg                                                                                                                       <  2:1354703/140‑1 (MQ=255)
                    aggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAAAAAAAGACCTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGa                                                                                                                      <  2:3583444/140‑1 (MQ=255)
                      ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                                    >  1:1563037/1‑140 (MQ=255)
                        agaAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATgg                                                                                                                  <  1:2378285/140‑1 (MQ=255)
                        agaAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATgg                                                                                                                  <  1:911031/140‑1 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCTTCGACAAAGAATGGAAAt                                                                                                              >  1:2696909/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                              >  1:2152485/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                              <  2:490169/140‑1 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                              <  2:605373/140‑1 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                              <  1:1789262/140‑1 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                              >  1:2045352/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATAGTCGACAAAGAATGTAAAt                                                                                                              >  1:4117923/1‑140 (MQ=255)
                                       tCAGGTAAATTGAATCCGATATCTCTTTCATCCGCAATTAATTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  1:4365270/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  2:1563037/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  1:129306/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  1:36633/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  2:809418/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  2:279809/140‑1 (MQ=255)
                                         aGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAcaa                                                                                                 >  2:1438174/1‑138 (MQ=255)
                                         aGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCa                                                                                                 >  1:666372/1‑140 (MQ=255)
                                         aGGGAAATTGAATCAGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCa                                                                                                 >  1:4729995/1‑140 (MQ=255)
                                           ggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGc                                                                                               <  2:2631360/140‑1 (MQ=255)
                                                    aTCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCa                                                                                      <  1:982853/140‑1 (MQ=255)
                                                     tCCGATATCTGTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCTCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCAt                                                                                     >  2:670790/1‑140 (MQ=255)
                                                     tCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAATGAATGGAAATCGGTAAGCGACCAGCTCGTGAGCAt                                                                                     >  2:1871026/1‑140 (MQ=255)
                                                     tCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   >  2:3489064/1‑126 (MQ=255)
                                                     tCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                   <  1:3489064/126‑1 (MQ=255)
                                                     tCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATAGGTACGCGACCAGCTCGTGAGCAt                                                                                     >  1:3215862/1‑140 (MQ=255)
                                                      ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                                    <  1:4435580/140‑1 (MQ=255)
                                                      ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATCCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                                    <  1:4784580/140‑1 (MQ=255)
                                                                     tCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCggg                                                                     >  2:862632/1‑140 (MQ=255)
                                                                      ccGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGt                                                                    <  2:3810761/140‑1 (MQ=255)
                                                                       cGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAcgg                                                                         <  1:2569643/134‑1 (MQ=255)
                                                                        gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                  <  1:3398958/140‑1 (MQ=255)
                                                                        gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                  <  1:189564/140‑1 (MQ=255)
                                                                        gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                  >  1:1207076/1‑140 (MQ=255)
                                                                         cAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGCTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTc                                                                 >  2:1200547/1‑140 (MQ=255)
                                                                          aaTTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                                >  2:319743/1‑140 (MQ=255)
                                                                          aaTTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                                >  2:4323095/1‑140 (MQ=255)
                                                                                  aaCAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                                        <  2:1303247/140‑1 (MQ=255)
                                                                                             tatttATGCGCGAGGTTGTTTTTTTGTTTCAAAAAAAGGGCAGATATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCCGCTCGTGAGCATGCGGGTAACCGGCGGGTTTCCATATTTGACAGTAAacgac                                             <  2:2277503/137‑1 (MQ=255)
                                                                                                   tGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAt                                       >  2:2737094/1‑140 (MQ=255)
                                                                                                   tGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAt                                       >  2:2330366/1‑140 (MQ=255)
                                                                                                   tGCGCGAGGATCTTTATTTGTTTAAAAAACAGTGAAGATATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGTCGGGTTTCAATATTTGACAGTAAACGACGGTGAt                                       >  2:307992/1‑140 (MQ=255)
                                                                                                    gcgcGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCa                                                                                      >  2:1216665/1‑92 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                          >  1:3996330/1‑55 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                                        <  2:1063697/121‑1 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                                     >  2:4938914/1‑140 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAGTa                                     >  2:721426/1‑140 (MQ=255)
                                                                                                         aGGATCTTTATTTGTTTCAAAAAAAGGCCAGAGATTATAAAATAGTCTACAAAAAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGTTAAAATGAGGGTGTCCATATTTGACAGTAAACGAAGGTGAATACATg                                 >  2:292453/1‑140 (MQ=255)
                                                                                                               tttATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAAt                           >  2:2033892/1‑140 (MQ=255)
                                                                                                                ttttttGTTTAAAAAACTGGGAATAGATTATAAAATCGTCGACAAATAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATTTTTGACATTAAACGACGGTGATTACATGAAAAATa                          <  2:1949222/137‑1 (MQ=255)
                                                                                                                ttATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATa                          >  2:1110293/1‑140 (MQ=255)
                                                                                                                ttATTTGTTGCAAAACCAGGGCTGAGATAATAAAATCTTCGACAAAGAATGGAAATCGGTACGCGACCAGATCGGGAGCATCCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATa                          <  2:3259838/140‑1 (MQ=255)
                                                                                                                ttATTTGTTACAAAACCCGGGCCGAGATTATAAAATCGGCGACAAAGAATGGAAATCGGTACGCGACAAGCTCGTCAGCATGCGGTAAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATa                          <  1:5088346/140‑1 (MQ=255)
                                                                                                                  ttttGTTAAAAAAACAGGGCAGATATTATAAAATCTTCTACAAACAATGCAAATCGTTACGCCACCAGCTCGTGAGAATGCGGTTAAACGGCGGGTTTCCATATTTGACCGTAAACGACGGTGATTACATGAAAAATAAc                        <  1:2009329/139‑1 (MQ=255)
                                                                                                                  aTTTTTTTAAAAAACTGGGCAGATATTAAAAAATCGTCTACAAAGATTGGAAATCGGTACGCGACCAGCTGTTGAGCATGGGGGTAAACGGCGGGTTTCCATTTTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  1:103526/140‑1 (MQ=255)
                                                                                                                  aTTTTTTTAAAAAACCGGGCAGAGTTTATAAAATCGTCGACAAATAATGGAAAGGGGTACGCGACCAGCTCTTGAGAATGCGGTTAACCGGCGGGTTTCAATATTTGACATTAAACGACGGTGATTACATGAAAAATAAc                        <  1:4323095/140‑1 (MQ=255)
                                                                                                                  aTTTTTTTAAAAAACAGGGCAGATTTTATAAACTCGTCGACAAAGATTGGAAATCGGTACGCGACCAGCTGGTGAGAAGGGGGGTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  1:2454737/140‑1 (MQ=255)
                                                                                                                  aTTTTTTTAAAAAACAGGGCACCTTTTATAAATCCGTCCAAAAATAATGCAAATGGGTACGCGACCAGCTCGTGAGCAGGGGGGTAAACGGCGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  1:790603/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTCAAAAACTGGGCACATATTAAAAAATCGTCCAAAACGAATGGAAATGGGTACGCGACCAGCTCGTGAGCTTGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  1:3717129/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTCAAAAACAGGCCAGATATTATAAAATCGTCGACAAAGAATGTAACTCGGTCCGCGCCCCGCTCGTGAGCTGGCGGGTAACCGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  1:3115226/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAAACTGGGAAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGATCGTGAGAATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  2:4747242/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAAACAGGGCATAGATTTTAAAATCGTCGACAAATAATGGAAATCGGTACGCGACCAGATCGTGAGCATGCGGTTAACCGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  2:2550174/140‑1 (MQ=255)
                                                                                                                  aTTTGTTCAAAAAACAGGGCAGAGTTTATAAAATCGTCGACAACGAATGCAAATCGGTACGCCACCAGCGCGTGAGAATGCGGGAAACCGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                        <  2:4611206/140‑1 (MQ=255)
                                                                                                                     tttttAAAAAAACGGGAATATATTATAAATTAGTCGACAAACATTGGAAATCGGTACGCGACAAGCTCTTGATAATGCGGTTAAACGGCGGTTTTCATTATTTGACATTACACGACGGTGATTACATGAAAAATAACGAg                     <  1:1869135/138‑1 (MQ=255)
                                                                                                                     tGTTTAAAAAACAGGGAAGACATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCTACCAGCTCGTGAGAATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAg                     <  2:4263796/140‑1 (MQ=255)
                                                                                                                     tGTTACAAAAACAGGTCAGATATTATAAAATCGTCTACAAATAATGTAAATGGGTACGCGACCAGCTGTTTAGCATGCGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAg                     <  2:3984214/140‑1 (MQ=255)
                                                                                                                      gTTTCAAAAACTGGGCATAGATTATAAAATCGTCGAAAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCAGGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                    <  2:745668/140‑1 (MQ=255)
                                                                                                                      gTTTAAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGATTGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                    <  2:4123453/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACTGGGCAGAGATTATAAAATCGTCGAAAAAGATGGGAAATCGGTACGCGACAAGCTCGTGCGCTGGGGGGTAAAGGGGGGGTTTCCATTTTTGAATGTAAACGACGGTGATTACATGAAAAATAACGAGct                   <  1:5149357/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACTGGGCAGAGATTATAAAATCGTCGAAAAAGAATGCAAATGGGTACGCGACCAGCTCGTCAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                   <  1:2995570/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACTGGGAATTGATTATAAATGGGTCGAAAAATAATGGAAATCGGTACGCTACCTGTTGTTCAGCATGGGGGTAAACGGCGGGTTTCCTTATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                   <  1:3091204/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACAGGGCAGATATTATAAAATCGTCGAAAAAGAATGCAAATCGGTCCGCGACCAGCTCGTGAGCATGGGGGTAACCGGCGGGTTTCAATATTTGACAGTACACGACGGTGATTACATGAAAAATAACGAGct                   <  1:388721/140‑1 (MQ=255)
                                                                                                                       tttAAAAAAATGGGCAGAGATTATAAAATCGTCGACAATGAATGGAAATCGTTACGCGACCTGCTCGTTTGCATGCGGTTAAAGGGCGGGTTTCCATTTTTGAAAGTAAACGACGGTGATTACATGAAAAATAACGAGct                   <  1:1078124/140‑1 (MQ=255)
                                                                                                                       ttacAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAGGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAACCGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                   <  2:920635/137‑1 (MQ=255)
                                                                                                                       ttacAAAAACAGGCCAGCTATTATAAAATCGTCGACAAAGAATGCAAATCGGTCCGCCACCAGCTCGTGAGCTTGCGGGTAAACGGCGGGTTTCCATATTGGACATTAACCGACGGTGATTACATGAAAAATAACGAGct                   <  1:1519295/137‑1 (MQ=255)
                                                                                                                       ttaaaaaaaaTGGCCAGATATTATAAATTGTTCTAAAATGAATGCAAATCGTTTCGCCACAAGCTCGTTCGCATGCGGTTAAACGGCGGGTTTCAATTTTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                   <  1:456104/136‑1 (MQ=255)
                                                                                                                              aaCAGGGCTGTTTTTTTAAATTCGTCCAAAAATAATGCAATTCGGTAGGCCACCAGCTCGTGAGCATGGGGTTAAAGGGCGGTTTTCAATATTTCACATTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt            <  1:4633538/140‑1 (MQ=255)
                                                                                                                              aaCAGGGCATATATTATAAAATCTTCGACAAACAAGGTAAATCGGTACGCAACAAGATCTTGACAATGCGGTAAAAGGGGGGGTTTCCATATTTCACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt            <  1:2884873/140‑1 (MQ=255)
                                                                                                                              aaCAGGGCATAGATTATAAAATCGTCGACAAAGACTGTAAATCGGTACGCGACCAGCTCGTGAGAATGGGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt            <  1:2822340/140‑1 (MQ=255)
                                                                                                                                   ggCATAGATTATAAAATCGTCCACAAAGAATGGAAAGCGGTACGCCACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa       <  1:4240968/140‑1 (MQ=255)
                                                                                                                                   ggCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTCCACGACGGTGATTACAGGAAAAATAACGAGCTCTCTATTAAGCa       >  2:2896402/1‑140 (MQ=255)
                                                                                                                                   ggCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa       >  2:4020597/1‑140 (MQ=255)
                                                                                                                                   ggCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa       >  2:5108870/1‑140 (MQ=255)
                                                                                                                                    gctccgaTTATAACATCGGCTCAAAAGATGGGAAATCTGTACGCGACCACCGGGTGCGCACGCGGGTAAACGGGGGGTTTCCAAATTTGACTGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCAt      <  2:2060046/135‑1 (MQ=255)
                                                                                                                                    gaagagATTATAAAATCGTCGACAAAGAATGGAAATGGGTCCGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCAt      <  1:4105388/138‑1 (MQ=255)
                                                                                                                                    gCATAGATTATAAAATCGTCGACAACGAATGGAAATCGGTACGCCACCAGCTCGTGAGAATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCAt      <  2:2465601/140‑1 (MQ=255)
                                                                                                                                    gCACAGATTATAAAATCGTCGACAAAGAATGTAATTCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGGGGGTTTCAATATTTGAAATTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCAt      <  1:3836465/140‑1 (MQ=255)
                                                                                                                                        agaTTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCAGTGGt  >  2:4370191/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCATTGGT  >  NZ_CP034943/986664‑986939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: