BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,040,043	A→C	5.2%	E431D (GAA→GAC)	EO946_RS05365 →	AAA family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,040,043	0	A	C	5.2%	724.9 / 7.3	301	E431D (GAA→GAC)	EO946_RS05365	AAA family ATPase
Reads supporting (aligned to +/- strand): ref base A (135/138); new base C (2/24); total (138/163)
Fisher's exact test for biased strand distribution p-value = 1.84e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

TACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGTTCATCGT  >  NZ_CP034943/1039906‑1040180

  cttACAGGCAATAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGAAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       <  2:4278655/138‑1 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTTAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  2:3867084/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       <  2:2422048/140‑1 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  2:223176/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  1:4047710/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  2:634049/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  1:1983714/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  1:3728014/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  2:3279887/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCg                                                                                                                                        >  1:3860321/1‑139 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCATTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       >  1:3156155/1‑140 (MQ=255)

  cGTACAGGCAAGAGACAGAAAACGGCAGATTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       <  2:5192841/140‑1 (MQ=255)

  cGTACAGGCAAGAGACACAAAAGGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       <  2:4711319/140‑1 (MQ=255)

   gTAAAGGAAAGAGAAAGAAAACGGCAGCTTTATGAGAAATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCTTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGc                                                                                                                                      <  2:1756704/140‑1 (MQ=255)

     aCAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGctc                                                                                                                                    >  2:3227218/1‑140 (MQ=255)

       aGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACACCGAAAGAAAAGATAAAGACGCGGctctg                                                                                                                                  >  2:2944858/1‑139 (MQ=255)

       aGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCtt                                                                                                                                  >  1:4986983/1‑140 (MQ=255)

        ggaaaGATACAGAAAACGGCAGATTTTTTAGACATTTGAACTTGCCCGTCAAGATCTGGAAGACGCAGAAAAGGTCGTTCATGCCGATTCGTCCGCTATCATTGAAAACAACGAAAGAAAACATAAAGAAGCGGCTCTTa                                                                                                                                 <  2:1579604/137‑1 (MQ=255)

        ggCACGAGACAGACAACGGCAGCTTGATGAGCCATTTGAACTGGCCCGGCAAGCGCTGGAAGACGCAGAAATGGTCGTTAAGGCCGCTTCGGCGGCTATCCTTGAAAACAACGAACGAAACCATACAGACGCGGCTCTTa                                                                                                                                 <  1:672586/140‑1 (MQ=255)

        ggCAATACACACAACACGGCATCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTTAAAACAACGAAAGAAAACATAAACAAGCGGCTCTTa                                                                                                                                 <  2:561623/140‑1 (MQ=255)

        ggCAAGAGACAGAAAACGGCAGCTTTATGAGACATTTGAACTTGCCCGGAAAGAGCTGTAAGACGCATAAATGGTCTTTCATGCCGCTTCGTCCGATATCCTTTAAAACAACGAAAGAAAATATAAACAAGCGGCTCTTa                                                                                                                                 <  1:1279468/140‑1 (MQ=255)

        ggCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       <  2:1982693/134‑1 (MQ=255)

        ggCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                       <  2:1801612/134‑1 (MQ=255)

        ggCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTa                                                                                                                                 <  2:696674/140‑1 (MQ=255)

           aaGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATCAAGAAGCGGCTCTTAAAg                                                                                                                              >  2:3024947/1‑140 (MQ=255)

           aaGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGACGCGGCTCTTAAAg                                                                                                                              >  2:3406213/1‑140 (MQ=255)

            agagACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGc                                                                                                                             >  2:3650201/1‑140 (MQ=255)

            agagACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGGTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAACGAAAAGATAAAGAAGCGGCTCTTAAAGc                                                                                                                             >  2:1041396/1‑140 (MQ=255)

             gagaCAGAAAACGGCAGCTTGTTGAGACATTTGAAGTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAACGAAAAGATAACGACGCGGCTCTTAAAGCa                                                                                                                            <  1:1168725/140‑1 (MQ=255)

               gaCAGAAAACGGCAGCTTGATGAGTCATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCata                                                                                                                          <  2:4004438/140‑1 (MQ=255)

               gaCAGAAAACGGCAGATTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAATGGTCGTTCATGCCGCTTCGTCCGCTATCATTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCata                                                                                                                          <  1:1228969/140‑1 (MQ=255)

                 cAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATatg                                                                                                                        >  2:2059078/1‑140 (MQ=255)

                 cAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATatg                                                                                                                        >  2:4561736/1‑140 (MQ=255)

                    aacaCGGCAGCTGGATCACACATTCGAACTGGCCCGGCAAGAGCGGGAAGACGACGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACACCGAAAGAACAGATAAAGAAGCGGCTCTTAAAGCATatgatg                                                                                                                     <  2:2868988/137‑1 (MQ=255)

                    aacaCGGCAGATTTATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCACAAAAGGTGGTTCATGCCGCTTCGTCCGCTATCCTTCAAAACAACCAAACAAAACATAAAGACGCGGCTCTTAAAGCATatgatg                                                                                                                     <  1:176770/137‑1 (MQ=255)

                    aaaaCGGCAGCTTTATGAGACATTTCAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTCAAAACAACGAAAGAAACCATAAACAAGCGGCTCTTAAAGCATatgatg                                                                                                                     <  1:4172088/140‑1 (MQ=255)

                    aaaaCGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATatgatg                                                                                                                     <  2:193450/140‑1 (MQ=255)

                     aaaCGGCAGCTTGATGAGAAATTTGAACTTGCCCGGCAAGAGCTGTAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGATATCCTTCAAAACAACGAAAGAAAATATAAACAAGCGGCTCTTAAAGCATAtgatga                                                                                                                    <  1:2094729/140‑1 (MQ=255)

                              cTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAggg                                                                                                           <  2:3156155/140‑1 (MQ=255)

                              cTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCATAAAGAATATGATGAAGCACAggg                                                                                                           <  2:3828806/140‑1 (MQ=255)

                                tgatgaTAAATTTGAAGTGGCCCGGCAAGAGATGTAAGACGCACAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTCAAAACAACGAAAGAAAAGATAACGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                         <  1:1364515/140‑1 (MQ=255)

                                tgatgaGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCATAAAAGGTCGTTCATGCCGATTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAACAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                         <  1:3314917/140‑1 (MQ=255)

                                tgatgaGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAACGATAAACAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                         <  2:812013/140‑1 (MQ=255)

                                tgatgaGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                         <  2:4088732/140‑1 (MQ=255)

                                tgatgaGACATTTGAACTTCCCCGGCAAGAGCTGGAACACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTCAAAACAACCAAACAAAAGATAAACACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                         <  1:1688748/140‑1 (MQ=255)

                                     agaattttGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTAATGCCGCTTCGTCCGCTATCCTTGAAAACAACCAAAGAAAACATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTAt                                                                                                    <  1:4323015/135‑1 (MQ=255)

                                     agaCATTTGAACTTGCCCGGAAAGAGCTGTAAGACGCAGAAACGGTCGTTCATGCCGCTTCGTCCGCTATCATTGAAAAAAACGAAACAAAAGACACAGACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTAt                                                                                                    <  1:2448967/140‑1 (MQ=255)

                                          tttGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGa                                                                                               <  2:5038186/140‑1 (MQ=255)

                                           ttGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAg                                                                                              >  1:4296928/1‑140 (MQ=255)

                                                 gtGCCCGGCCAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCg                                                                                        <  2:3701904/139‑1 (MQ=255)

                                                  tGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGa                                                                                       >  2:3948692/1‑140 (MQ=255)

                                                  tGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATACTTGAAAACAACGAAATAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGa                                                                                       >  1:4884116/1‑140 (MQ=255)

                                                   gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCg                                                                                                                                        <  2:497359/90‑1 (MQ=255)

                                                   gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                                      >  2:3729430/1‑140 (MQ=255)

                                                   gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                                      >  2:396510/1‑140 (MQ=255)

                                                   gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                                      >  2:334076/1‑140 (MQ=255)

                                                   gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                                      >  1:4388154/1‑140 (MQ=255)

                                                        gCAAGAGCTGGAAGACGCAGAAAAGGTCTTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAACGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTga                                                                                 <  2:4341854/140‑1 (MQ=255)

                                                        gCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTga                                                                                 <  2:2001584/140‑1 (MQ=255)

                                                        gCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTga                                                                                 <  2:480221/140‑1 (MQ=255)

                                                                  gAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCa                                                                                                        <  2:3533171/107‑1 (MQ=255)

                                                                  gAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCAGATGATGAAGCACAGGGGCa                                                                                                        >  1:3533171/1‑107 (MQ=255)

                                                                   aaGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTg                                                                      >  2:749462/1‑140 (MQ=255)

                                                                       cGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGca                                                                                                                <  1:2686088/94‑1 (MQ=255)

                                                                                 gTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAg                                                        >  1:2831571/1‑140 (MQ=255)

                                                                                 gTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAg                                                        >  2:4187018/1‑140 (MQ=255)

                                                                                 gTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAg                                                        >  1:346485/1‑140 (MQ=255)

                                                                                    gTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGgca                                                     >  2:5049794/1‑140 (MQ=255)

                                                                                    gTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGgca                                                     >  1:2958556/1‑140 (MQ=255)

                                                                                         tGCCGCTGCGTCCGCTATCCTTGAAAACAACGAAACAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAgg                                                <  1:218637/140‑1 (MQ=255)

                                                                                              cTTCGTCCTCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTaaa                                           <  2:2281338/140‑1 (MQ=255)

                                                                                              cTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTaaa                                           >  2:2744685/1‑140 (MQ=255)

                                                                                              cTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTaaa                                           >  2:2666785/1‑140 (MQ=255)

                                                                                              cTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTaaa                                           <  2:594247/140‑1 (MQ=255)

                                                                                               ttCGTCCGCTATCCTTGAAAAGACGGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGGTGAAGCAGAGGGGCAGTATCAGGAGCACGCGAAGCGGCGAGAGCAGCGGTGGTGTTGTGAAAGGCAGCAGGCTATAc                                          >  2:2032862/1‑140 (MQ=255)

                                                                                                   tCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGaa                                      >  2:1366940/1‑140 (MQ=255)

                                                                                                    ccGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGaaa                                     <  2:2614519/140‑1 (MQ=255)

                                                                                                    ccGATATCCTTCAAAACAACGAAACAAAACAAAAACACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGaaa                                     <  1:1899288/140‑1 (MQ=255)

                                                                                                        tATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAgg                                 >  1:4843569/1‑140 (MQ=255)

                                                                                                           ccTTGAAAACAAGGAAAGAAAAGATAAAGACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTg                              <  2:4885668/140‑1 (MQ=255)

                                                                                                           ccTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTg                              <  2:534798/140‑1 (MQ=255)

                                                                                                            cTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATAGGCTGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGt                             <  2:1270561/140‑1 (MQ=255)

                                                                                                             ttGAAAACAACGAAAGAAAAGATAAATAAGCGGATCTTAAAGCATAGGATGAAGCACAGGGGCAGTATCTGGAGCAATCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAATGGCATCAGGCTAAACAGAAATGGGTTGTc                            >  1:2479873/1‑140 (MQ=255)

                                                                                                             ttGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTc                            >  2:1726618/1‑140 (MQ=255)

                                                                                                             ttGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTc                            >  1:2078935/1‑140 (MQ=255)

                                                                                                             ttGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCAGATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTc                            >  1:1450719/1‑140 (MQ=255)

                                                                                                               gAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCAt                          >  1:4474732/1‑140 (MQ=255)

                                                                                                                 aaaCAACGAAAGACAAGATAACGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTg                        <  2:3438932/140‑1 (MQ=255)

                                                                                                                  aacaacGAAAGAAAAGATAAAGACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                       <  1:6057/140‑1 (MQ=255)

                                                                                                                  aacaacGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                       <  1:2971128/140‑1 (MQ=255)

                                                                                                                  aacaacGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                       <  1:711289/140‑1 (MQ=255)

                                                                                                                    caacGAAAGAAAAGATAAAGAAGCGGCGCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAg                     >  1:932409/1‑140 (MQ=255)

                                                                                                                         aaagaaaagaTAACGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt                <  2:2592819/140‑1 (MQ=255)

                                                                                                                         aaagaaaagaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt                <  2:2543660/140‑1 (MQ=255)

                                                                                                                         aaacaaaataTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt                <  2:3423614/140‑1 (MQ=255)

                                                                                                                         aaacaaaagaTACAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt                <  1:5176187/140‑1 (MQ=255)

                                                                                                                                   tacagaagCGGCTCATAAAGCATATGATGAAGCACAGGGGCAGTCTCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGTTCa      <  1:3248748/137‑1 (MQ=255)

                                                                                                                                    aaaGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGTTCAt     <  1:475336/140‑1 (MQ=255)

                                                                                                                                    aaaGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGTTCAt     <  2:735653/140‑1 (MQ=255)

                                                                                                                                      agaagCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGTTCATCg   <  1:2609297/140‑1 (MQ=255)

TACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGTTCATCGT  >  NZ_CP034943/1039906‑1040180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`