Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,225,192 C→A 9.5% intergenic (‑71/‑48) EO946_RS06345 ← / → EO946_RS06350 ATP‑dependent helicase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,225,1920CA 9.5% 637.0 / 6.9 269intergenic (‑71/‑48)EO946_RS06345/EO946_RS06350ATP‑dependent helicase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (114/118);  new base A (2/35);  total (116/153)
Fisher's exact test for biased strand distribution p-value = 1.29e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AATTGGTAATGTTCTCTCGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTACGCCGGAACCGAAAA  >  NZ_CP034943/1225054‑1225331
                                                                                                                                          |                                                                                                                                           
  ttGGTAATGTTCTCTCGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCACAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCaaa                                                                                                                                          >  2:1490797/1‑140 (MQ=255)
      tAATGTTCTCTCGAATTTGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAaagt                                                                                                                                      <  2:673878/140‑1 (MQ=255)
      tAATGTTCTCTCGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAaagt                                                                                                                                      <  2:3084518/140‑1 (MQ=255)
          gTTCTCTCGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCCAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAaagtaact                                                                                                                                  >  2:3125244/1‑138 (MQ=255)
           ttCTCTCGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTAAAAAAGTAAGtt                                                                                                                                 >  2:4486655/1‑140 (MQ=255)
             ctctcGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTa                                                                                                                               <  1:2289600/140‑1 (MQ=255)
              tctcGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTaa                                                                                                                              >  2:2543800/1‑140 (MQ=255)
              tctcGAATATGGATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTag                                                                                                                              >  2:1110761/1‑139 (MQ=255)
               ctcGAATATGTATGTAACTGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAg                                                                                                                             <  1:1042280/140‑1 (MQ=255)
                    atatGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGaaa                                                                                                                        <  1:3941468/140‑1 (MQ=255)
                       tgtatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAgg                                                                                                                     <  1:4953666/140‑1 (MQ=255)
                       tgtatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTACAAATAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAgg                                                                                                                     <  1:5136808/140‑1 (MQ=255)
                         tatgtaAATTTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAATGGaa                                                                                                                   <  2:3599566/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATGTTTGAGTCGGGCGCATTTAAAAAAGGGATCCGCACAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAATCGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGaa                                                                                                                   <  1:4866030/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATCATTCAGTGGTGATCATTTCAAAAAGGTATCCTCCCAATACAAAAAGGTTTTCATTACAGACAAAGCCTATCATATCAATAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGaa                                                                                                                   <  2:3264866/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAATGTGTTCATTACAGACAAAGACTTTCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGaa                                                                                                                   <  2:4422657/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTACGTTTAAGGGAAAAGGaa                                                                                                                   <  2:4520492/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGaa                                                                                                                   <  1:3763989/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGaa                                                                                                                   <  2:5095498/140‑1 (MQ=255)
                         tatgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGaa                                                                                                                   <  2:526312/140‑1 (MQ=255)
                          atgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTCAGGTAAAAGGacc                                                                                                                  >  2:4632365/1‑138 (MQ=255)
                          atgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGacc                                                                                                                  >  2:2239505/1‑138 (MQ=255)
                          atgtaAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAAc                                                                                                                  >  2:3419895/1‑140 (MQ=255)
                              aaaTGTATAGTTCTATCATTCAGTCGGGCGCATTTCAAAAAGGTATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAATCCTATCATATCAAAAGAAACGTGTTTCAAAAAGTATGTTTAAGGGAAATGGAAcagca                                                                                                              <  2:4665434/140‑1 (MQ=255)
                              aaaTGTATAGTTCTATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTAATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTAAAAAAGTAAGTTTAAGGGAAAAGGAAcagca                                                                                                              <  1:62222/140‑1 (MQ=255)
                              aaaGGTATAGTCCGATAATTACGTCGTGCGAATTTCAAAATGGGATCCGCCCAATCAAAAAAGTTGTTAATAACTTACAAAGACTATCATATCAAAAGAAAGTTGTTTAAAACATTAAGTTTAATGgaaaaggaaaagaa                                                                                                              <  2:2405002/140‑3 (MQ=255)
                                atttataGTTCTATCATTCAGTCGGGCGAATTTCAAAAAGGGATCCGCCCAATCAAAAAAGGTGTCCATTACAGACAAAGACTATCATATAAAAAGAAACGTGTTTAAAAAAGTAAGTTTAAGGGAAAAGGACCAGCaga                                                                                                            <  2:265403/137‑1 (MQ=255)
                                aTGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGaaa                                                                                                                        >  2:3745699/1‑128 (MQ=255)
                                     taGTTCGATCATTCAGTCGGGGGAATTTAAAAACTGGATCCTCCAAATCAAAAAAGGTGTTCATTACACAAAACGCCTATCATATCAAACGAAACGTGTTTCAAAAAGTAAGTTTAAGGTAAAAGGAACAGCAGAGgggt                                                                                                       <  2:3509755/140‑1 (MQ=255)
                                     taGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGACAAGGAACCGCAGAGgggt                                                                                                       >  1:1380726/1‑140 (MQ=255)
                                     taGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGgggt                                                                                                       >  1:4128890/1‑140 (MQ=255)
                                       gTTCGATCATTCAGTCGGGCGCATTTCAAAAATGTATCCTCCCAATCAAAAAAGGTGTTCATTCAAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGgggtgg                                                                                                     <  1:147793/140‑1 (MQ=255)
                                       gTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGACCAGCAGAGgggtgg                                                                                                     >  2:4105959/1‑140 (MQ=255)
                                          ctaTCATTCAGTCGGGCGCATTTCAAAAAGGGATCATCCAAATCAAAAAAGGTTTTCATTCCAGACAAAGACTATCATATCAAAAGAAACGTGTTTAAAAAAGTAAGTTTAGGGGAAAAGCAACAGCAGAGGGGTGGGTa                                                                                                  <  1:4575400/138‑1 (MQ=255)
                                          cGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTa                                                                                                  >  1:3269495/1‑140 (MQ=255)
                                          cGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTa                                                                                                  >  1:3086511/1‑140 (MQ=255)
                                          cGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGGTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTa                                                                                                  >  1:1535166/1‑140 (MQ=255)
                                          cGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCGCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTa                                                                                                  <  1:581679/140‑1 (MQ=255)
                                          cGATCATTCAGTCGGGCGAATTTAAAAACGGGATCCTCCCAATCAAAAAAGGTGTTCTTTACAGACAAAGACTATCATATCAAAACAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGCAACAGCAGAGGGGGGGGTa                                                                                                  <  1:951173/140‑1 (MQ=255)
                                          cGATCATTCAGTCGGCCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTCCATTACAGACAAACACTATCATATCAAACGAAAGGTTTTTAAAAAATTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTa                                                                                                  <  2:1959040/140‑1 (MQ=255)
                                           gATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTCTCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGACCAGCAGAGGGGTGGGTAt                                                                                                 >  2:1703747/1‑140 (MQ=255)
                                            aTCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATc                                                                                                >  2:1602015/1‑140 (MQ=255)
                                            aTCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATc                                                                                                >  1:711675/1‑140 (MQ=255)
                                              cATTCCGTCGGGCGCATTTAAAAAAGGGACCCTCCCAATCAAAAAAGGTGTTAATTACAGACAAAGACTAGCATATCAAAAGAAACGTGTTTAAAAACGTACTTTTAAGGCAAAAGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:2140255/140‑1 (MQ=255)
                                              cATTCAGTCGGGCTAATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAACGAAACGTGTTTCAAAAAGTACGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:4099958/140‑1 (MQ=255)
                                              cATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCCTATCAAAAGAAACGTGTTTCAAAAAGTACGTTTAAGGGAAAAGGAACAGCAGCGGGGTGGGTATCGt                                                                                              <  2:4191839/140‑1 (MQ=255)
                                              cATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:1812257/140‑1 (MQ=255)
                                              cATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTAAAGGCAAACGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:4390620/140‑1 (MQ=255)
                                              cATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGGGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAACGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:4158427/140‑1 (MQ=255)
                                              cATTCAGTCGGGCGCATTTAAAAAAGGGATCCTCCCAATCAAAAAAGGTTTTCATTACACACAAACACTATCATATCAAACCAACCGTGTTACAAAAAGTAAGTTTACGGCAACCGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:508883/140‑1 (MQ=255)
                                              cATTCAGTCGGGCGAATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCTTTACATACAAAGACTATAATATCAAAAGAACCGTTTTTCAAAACGTACGTTTAAGGGAAAAGCAACAGCAGAGGGGTGGGTATCGt                                                                                              <  1:333663/140‑1 (MQ=255)
                                              atttCAGTCGCGCGCATTTCAAAAAGGTATCCTCCAAATCAAAATATGTGTTCATTACTTACAAACAATATCATATCAAAAGAAACGTGTTTAACAACTTAACTTTAAGTGAAAAGTACCTGCTGTGGGGTGGGTATCGt                                                                                              <  2:4298816/138‑1 (MQ=255)
                                              aaTTCCGTCGGGCGCATTTCAAAAAGGGAGCCGCCCAATCAAAAAAGGTGTTCATTACAGACAAAGAATAACATATCAAACGAACCGTGTTTCAAAAAGTAAGTTTAAGGCAAACCCACCAGCAGAGGGGTGGGTATCGt                                                                                              <  2:762913/139‑1 (MQ=255)
                                              aaTTCAGTCGGGCTCTTTTAAAAATGGGATCCTCCAAAAAAAAAAAGGTGTTCATTACATACAACCACTAAAATATCAAAACAAACGTGTTTAAAAATGTAAGTTTAAGGGAAAAGGAACAGCAGTGGGGGGGGTATCGt                                                                                              <  2:5146814/139‑1 (MQ=255)
                                              aaTTCAGCCGGGCGAATTACAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTAATTACAGACAAACACTATCATATAAAAACAAACGTTTTAAAAAAATTACGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGt                                                                                              <  2:836091/139‑1 (MQ=255)
                                                ttCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAATGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAATGGAACAGCAGAGGGGTGGGTAGCGTAt                                                                                            >  2:4269363/1‑140 (MQ=255)
                                                ttCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTAt                                                                                            >  2:2915940/1‑140 (MQ=255)
                                                  cAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGa                                                                                          >  2:786503/1‑140 (MQ=255)
                                                   attCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAACGGTGTACATTACAGAAAAAGACTATCATATCAAAACAAACGTGTTACAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGat                                                                                         <  2:2075307/138‑1 (MQ=255)
                                                   aGTCGGGCGCATTTCAAAAAGGCATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGat                                                                                         <  2:4610800/140‑1 (MQ=255)
                                                            cATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAgg                                                                                >  1:3890363/1‑140 (MQ=255)
                                                              tttCAAAAAGGGATCCTCCAAATCAAAAAAGGTGTTCATTACATACAAAGAATATCATATAAAAATAAACGGGTTTAAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGTTGGGTATCGTATGATATATGCAGGTc                                                                              <  2:4965002/140‑1 (MQ=255)
                                                              tttCAAAAAGGGAGCCGCCCTATCAAAAAAGGTGTTCATTACAGACAAAGACTATCTTATCAAAATAAACGTGTTTCAAAACGTAAGTTTAAGGGAAAAGGAACAGCAGTGGGGTGGGTATCGTATGATATATGCAGGTc                                                                              <  1:4444388/140‑1 (MQ=255)
                                                               ttCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGgggt                                                                                                       >  1:3325112/1‑114 (MQ=255)
                                                                    aacggtaTCCCCCAAATCAAAAATGGTGTTCATTAAACAAAACCACTATCATATAAAAAGAACCGTTTTAAAAAAAGAAAGTTTAAGGCAAAAGCAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTc                                                                        <  2:2344037/134‑1 (MQ=255)
                                                                         gATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAAc                                                                   >  1:1663394/1‑140 (MQ=255)
                                                                         gATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAAc                                                                   >  1:2966091/1‑140 (MQ=255)
                                                                         gATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAAc                                                                   >  1:1174756/1‑140 (MQ=255)
                                                                         gATCCTCCCAATCAAAAAAGGTGTTCATTACACAAAAACACTATAATATAAAACGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGCAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAAc                                                                   <  1:3390802/140‑1 (MQ=255)
                                                                         gATCCTCCAAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCAAATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGTAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAAc                                                                   <  2:70268/140‑1 (MQ=255)
                                                                              tccCAATCAAAAAAGGTGTTCATTACAGACACTGACTATCATATCCAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGCAATGTAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAGAGTCAGAACCAGCa                                                              >  2:2897133/1‑140 (MQ=255)
                                                                                            ggTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCACAATATg                                                >  1:3736683/1‑140 (MQ=255)
                                                                                              tgtTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCa                                              >  2:2859910/1‑140 (MQ=255)
                                                                                                ttatttACATACAAAGACTATCATATCACAATAATCGTGTTTAAAATAGTAATTTTAAGGTACAAGGAACAGGAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCCGAACCAGCAATACGCTCAATAGGCaaa                                            <  2:1531985/136‑1 (MQ=255)
                                                                                                ttCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCaaa                                            <  1:2726858/140‑1 (MQ=255)
                                                                                                ttCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCaaa                                            >  1:4662263/1‑140 (MQ=255)
                                                                                                ttCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCACAATATGCaaa                                            >  1:4522929/1‑140 (MQ=255)
                                                                                                ttCATTACAGACAAAGACTATCATATCAAAAGAAACGTGGTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTCTCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATAAGCTCAATATGCaaa                                            >  1:3475687/1‑140 (MQ=255)
                                                                                                   aTTACATACAAAGACTATCATAACAAACGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAg                                         <  2:4819886/140‑1 (MQ=255)
                                                                                                   aTTACAGAAAAATACTATCATATCAAAATAAACGTGTTACAAAATGTATTTTTAAGGTAAAAGGAAAAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAg                                         <  2:2273424/140‑1 (MQ=255)
                                                                                                        aGACAAAGCCTATCATATCAAACCAAACGTGTTTCAAAACGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGGGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGa                                    <  2:4393087/140‑1 (MQ=255)
                                                                                                            aaaGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTgg                                >  2:1005551/1‑140 (MQ=255)
                                                                                                              aGACTATCATATCAAAAGAAACTTGTTTAAAAAAGTCCGTTTAAGGGAAAAGGACCAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATAGGCAAATAGAACTATTGGac                              <  1:257408/140‑1 (MQ=255)
                                                                                                                  tatcatatcaAAACAAACGTGTTTCAAACAGTAAGTTTAAGGGAAAAGCAACAGCCGAGGCGTGGGTATCTTATGATATATGCAGGTCAACTTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACaa                          <  1:2995684/140‑1 (MQ=255)
                                                                                                                   atcatatcaAAAGAACCGTGTTTCAAAAAGTACGTTTAAGGGAAAAGCAACAGCAGCGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  1:2048680/140‑1 (MQ=255)
                                                                                                                   atcatatcaAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  2:1013786/140‑1 (MQ=255)
                                                                                                                   atcatatcaAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  1:276988/140‑1 (MQ=255)
                                                                                                                   atcatatcaAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  1:4018187/140‑1 (MQ=255)
                                                                                                                   atcatatcaAAAGAAACGTGTTACAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  1:1605691/140‑1 (MQ=255)
                                                                                                                   ataatatcaAAATAAACGTGTTTCAAAATGTAATTTTAAGTCAAAAGCAACAGCAGAGGGGGGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  2:2175203/137‑1 (MQ=255)
                                                                                                                   ataatataaAAACAACCGTTTTTAAAAAAGAACGTTTAAGGCAAAAGCAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  1:1007535/137‑1 (MQ=255)
                                                                                                                   accatatcaAAACAAACGTGTTTAAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  2:4400198/138‑1 (MQ=255)
                                                                                                                   aacatataaAAACAAACTTGTTCAAAAATGTACGTTTAAGGGAAAAGTAACAGCTTAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAg                         <  2:3826863/138‑1 (MQ=255)
                                                                                                                          caAAAGAAACGTGTTTCAAAAATTAAGTTTATGGGAAATGTAACCGCAGTGGGGTGGTTATCGTATTATATATCCAGGTCAAATACTGAACAACAAATACGAGCAAAATGCAAAATGAACTATGGGAACCAACCAGGAta                  >  1:2546583/1‑139 (MQ=255)
                                                                                                                          caAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGAtt                  >  1:4335447/1‑140 (MQ=255)
                                                                                                                            aaaGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTAc                >  2:4146831/1‑140 (MQ=255)
                                                                                                                            aaaGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTAc                >  2:3343594/1‑140 (MQ=255)
                                                                                                                              acaaccGTGTTTAAAAACGTAAGTTTAAGGCAAACGGAACAGCAGCGGGGGGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTACGc              <  1:2088975/135‑1 (MQ=255)
                                                                                                                                  aCGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTACGCCGGa          >  1:4217599/1‑140 (MQ=255)
                                                                                                                                      ggtacAAAAAGTATGTTTAAGGTAAACGGAACAGCAGCGGGGTGGTTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTACGCCGGAACCg      <  2:2506451/136‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AATTGGTAATGTTCTCTCGAATATGTATGTAAATGTATAGTTCGATCATTCAGTCGGGCGCATTTCAAAAAGGGATCCTCCCAATCAAAAAAGGTGTTCATTACAGACAAAGACTATCATATCAAAAGAAACGTGTTTCAAAAAGTAAGTTTAAGGGAAAAGGAACAGCAGAGGGGTGGGTATCGTATGATATATGCAGGTCAAATTCAGAACCAGCAATACGCTCAATATGCAAATAGAACGATTGGACACAAGCAGGATTACGCCGGAACCGAAAA  >  NZ_CP034943/1225054‑1225331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: