Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,488,182 G→T 6.2% A575S (GCA→TCA)  ade → adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,488,1820GT 6.2% 406.8 / 2.8 140A575S (GCA→TCA) adeadenine deaminase
Reads supporting (aligned to +/- strand):  ref base G (50/78);  new base T (9/3);  total (59/81)
Fisher's exact test for biased strand distribution p-value = 2.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGA  >  NZ_CP034943/1488044‑1488320
                                                                                                                                          |                                                                                                                                           
cATGATAAATTGTTATTAGTCGGTTTCACATGCGGGTTTAATCCAATTTTGACGCTGTCGTTTTTATATTTGCCTGTAATTACTGACATCAAAATTAAGACAAAAGGATTATTTCTTTTAAAAACATTTCAGCATattgc                                                                                                                                            >  1:199472/1‑140 (MQ=255)
    aTAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTATCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATAATTTCAGCATatttcattg                                                                                                                                        >  1:3914979/1‑140 (MQ=255)
      aaaTTGTTATTAGTGGGTTTCACAGGCGGTTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTCACATCAAAACGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATAttgcattgca                                                                                                                                      <  2:2300263/140‑1 (MQ=255)
      aaaTTGTTATGAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATAttgcattgca                                                                                                                                      <  2:1567567/140‑1 (MQ=255)
        aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTTAAATCAAAATGACGACAACAGGATTATTTTATGTAAAATCATTTCAGCATATTTCATTGCaaa                                                                                                                                    >  2:1267587/1‑139 (MQ=255)
          tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTTCATGTCATTACTGACATAAAAATGAAGACAACAGGATTAATTGATGTAAAATCATTTAAACATATTGCATTTAAATaa                                                                                                                                  >  2:3132000/1‑140 (MQ=255)
          tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGATTTTCCTGTCAATCCTGACATAAAAATGACGACAACAGGATTATTTTAAGTAAAAAAATTTAAGCAAATAGCAATGCAATaa                                                                                                                                  >  1:1072529/1‑140 (MQ=255)
           gTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATaaa                                                                                                                                 <  1:1719587/140‑1 (MQ=255)
            ttattaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGGTTTGCCTGTAATTCCTGACATCAAAATGACGACAACAGGTTTTTTTGTTGTAAAATCATTTGAGCATAATTCATTTCAATaaaa                                                                                                                                >  1:1248334/1‑140 (MQ=255)
            ttattaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTCCTGACATCAAAAAGACGACAACAGGATTATTTGATGTAAAATAATTTCAGCAAATTTAATTGCAATaaaa                                                                                                                                >  1:1363018/1‑140 (MQ=255)
              attaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTTCCTGTCATTCCTGACATCAAAAAGACTACAAAAGGATTTTTTGATGTAAAAAAAATTCAGCAAATTTACTTTCAaaaaaaaa                                                                                                                              >  1:4898353/1‑140 (MQ=255)
                 aGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTTGCGTTGGCTGTCATTCCTGACATCAAAATGAAGACAACAGGATTATTTTATGTAAAATAATTTCAGCAGATTGCATTGCAATAAAAAAAGa                                                                                                                           >  1:3685963/1‑140 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:4633108/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:3746000/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:1541814/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  2:2636076/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:814741/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  2:4243582/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:1732918/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTGTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  2:2823669/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTAGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTAATTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  2:1748455/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCAATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:5178664/140‑1 (MQ=255)
                  gTCGCTTTCAGAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                          <  1:2747497/140‑1 (MQ=255)
                  gTCGCTTTCACACGCGGGTTTAATCCATTGTTGACCCTGTCGTATTTCGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATTTAAAATCATTTCAGCATATTGCATTTCAATAAACAAAGAt                                                                                                                          <  2:2938718/140‑1 (MQ=255)
                   tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                         <  2:4834195/140‑1 (MQ=255)
                    cgttttCACAGGCGTGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTTTCATTCCTGACATCAAAATGAAGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                        <  2:3285319/137‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                        <  2:1874430/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                        <  2:474481/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGACTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                        <  2:466545/140‑1 (MQ=255)
                    cGGGTTCCCAGGGGGGTTTAATCGATTTTGGACGCTGTCGTTTTTAGCTGTGGCTGTCATTCCTGACATCAAAATGACGACTACAGGATGATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                        <  2:2772037/140‑1 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTTCCTGGCCATCCGGACAACAAAATTTAGACAACAGGATTATTTTATGTAAAATAATTTAAGCATATTGGATTGCAATAAAAAAATATGCAc                                                                                                                      >  1:5222271/1‑140 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCAc                                                                                                                      <  2:3739520/140‑1 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTGTTAGCGTTGCCTTTCATTACTGACATCAAAAATACGACAACCCGATTATTTGTAGTAAAAAAAATTAAGCATATTGGATTGCAATAAAAAAAGATTCAc                                                                                                                      >  1:1609869/1‑140 (MQ=255)
                        ttCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTc                                                                                                                    <  1:1477949/140‑1 (MQ=255)
                         tCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTcc                                                                                                                   <  2:1555863/140‑1 (MQ=255)
                            caGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAACTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCaaa                                                                                                                <  1:1416537/140‑1 (MQ=255)
                              tgCGGGTTTAATCCATTTTTGACGCGGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAagt                                                                                                              <  2:2684082/139‑1 (MQ=255)
                                 gggTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATAAAAATGAAGACAACAGGATTATTTGATTTAAAAAAATTTCAGCATAATGCATTGCAATAAAAAAAGATGCACTCCAAagtgag                                                                                                           >  2:740972/1‑140 (MQ=255)
                                 gggTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCGGTCAATCCTGACATCAAAATGCCGACACCAGGTTTATTTGATGTAAATTAATTTCAGGATAATGCAATGCAATAAAAAAAGATTCCCTCCAAgctgag                                                                                                           >  2:2202933/1‑140 (MQ=255)
                                 gggTTTAATCCATTTTTGACGCTGTCGTGTTTAGCGTTGCCTGTCATTCCGGGCATCAAAATGTCGACAACTGGTTTATTTGATGTAAAATCATTTCAGCATTTTGGACTGCCATAATATGAGTTGGACTCCatcggggg                                                                                                           >  2:2929009/1‑133 (MQ=255)
                                        aTCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCt                                                                                                   <  1:4500673/140‑1 (MQ=255)
                                         tCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAGAGAt                                                                                                                          <  1:3466917/117‑1 (MQ=255)
                                             tttttGACGCTGTCGTTTTTAGATTTGGCTGTGATTCCTGACATAAAAATGACGAGAACAGGATTATTTGTTGTAAAATCATTTGAGCATATTTGATTGCAATTAATAAATATGGGATCCAAAGTGAGTGGATGtgtttt                                                                                              >  1:3222363/1‑140 (MQ=255)
                                             tttttGACGCTGTCGTGTTTTGCGTTTGCTGTCATTACGGACAACAAAATGACGACAACAGGATTATTTTATGTAAAATAATTTCAGCATATTTCATTGCAATAAAAAAAAATTCACTACAAAGTGATTTCATCtttttt                                                                                              >  1:569187/1‑140 (MQ=255)
                                                ttGACGCTGTCGCTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGCTGTAAAATCATTTCAGCATAGTGCATTGCACTAAAAAAAGATGCACTCCAAAGGGAGTGCATATTTTTTTTa                                                                                           <  2:3318700/140‑1 (MQ=255)
                                                  gACGCTGTCGTTGTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTGGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTaaa                                                                                         <  1:3574332/140‑1 (MQ=255)
                                                   aCGCTGTCGTTTTTAGCTTTTCCTGTCATTACTGACAAAAAAATTACGACAACAGGATTATTTTATGTAAAATAAATTCATCAAATTTCAATTCAATAAAAAAAGATTCACACCAAAATGAGTGCATCTTTTTTTTaaaa                                                                                        >  1:429507/1‑139 (MQ=255)
                                                   aCGCTGTCGTTTTTAGCTTTGCCTTTCATTCCTGACATCAAAATTAAGACAAACGGATTATTTTATGTAAAAACATTTAAGCAAAATGCATTGCAAAAAAAAAAAATTAACTCCCAATTGAGTGGAACATTTTTTTaaaa                                                                                        >  1:1956608/1‑139 (MQ=255)
                                                          cGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCatg                                                                                 <  2:1971423/140‑1 (MQ=255)
                                                          agTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCatg                                                                                 <  2:5191871/139‑1 (MQ=255)
                                                                tAGCTTTGGCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATc                                                                           <  2:4602875/140‑1 (MQ=255)
                                                                tAGCGTGGCCTGTCATTCCTGACATCAAACTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATCTTGCATTCCAATAAAAAAAGATGCACTCCAAAGTGAGTGCAACTTTTTTTTAAACGTCCATGATGATc                                                                           <  2:2404628/140‑1 (MQ=255)
                                                                gaGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATc                                                                           <  2:3295657/139‑1 (MQ=255)
                                                                 ttcattGCCTGTCATTACTGCCATAAAAATTAAGACAACACGATTATTTGATGTAAAATCAATTCACCAAAATGCATTTCAATAAAAAAATATGAACAAAAAAGTAATGGCAAAATTTTTTTAAAATTACATTATGATCg                                                                          >  2:4414213/5‑140 (MQ=255)
                                                                   cTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTTCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATGATTTTTTTAAACTTCCATTATGATCggt                                                                        >  2:2623700/1‑139 (MQ=255)
                                                                   cTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGa                                                                        <  1:880043/140‑1 (MQ=255)
                                                                     ttGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAg                                                                      >  2:3554061/1‑140 (MQ=255)
                                                                      tGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCCAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAgg                                                                     >  1:426191/1‑140 (MQ=255)
                                                                       gCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTTAAACTTCCATGATGATCGGAAgg                                                                     >  1:1208754/1‑140 (MQ=255)
                                                                             cATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCg                                                              >  1:2381941/1‑140 (MQ=255)
                                                                                      cATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtt                                                     >  2:373481/1‑140 (MQ=255)
                                                                                      cATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATAGGAAGGATCATCGGACGGcgtt                                                     >  2:4309700/1‑140 (MQ=255)
                                                                                       aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAGAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                                                    <  2:2724245/140‑1 (MQ=255)
                                                                                       aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                                                    <  2:1045868/140‑1 (MQ=255)
                                                                                       aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                                                    <  2:790919/140‑1 (MQ=255)
                                                                                       aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCAGTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                                                    <  2:3997074/140‑1 (MQ=255)
                                                                                          aaaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgt                                                 >  1:2879987/1‑140 (MQ=255)
                                                                                          aaaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgt                                                 >  1:4151086/1‑140 (MQ=255)
                                                                                          aaaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgt                                                 >  1:5185478/1‑140 (MQ=255)
                                                                                               gacgacAACAGTATTATTTGATGTAAAATCATTTCAGCCTAGTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:3815076/140‑1 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGTTGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACGCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:882539/140‑1 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGGATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  1:4856122/140‑1 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:4386391/140‑1 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  1:1019516/140‑1 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  1:2022380/140‑1 (MQ=255)
                                                                                                    aaacaGTATTATTTTCTGTAAAATCATTTCAGCATATTGCTTTGCAATAAAAAAAGATGCACTCAAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCatat                                       <  2:1179081/139‑1 (MQ=255)
                                                                                                     aacaGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCAttta                                      >  2:3399209/1‑140 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:574197/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:2838116/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:1681302/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:3132000/140‑1 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc                            >  1:1612173/1‑140 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCAGTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTGTTGCATATAAGTACGgcgc                            >  1:1859335/1‑140 (MQ=255)
                                                                                                                   aTGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAATATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGc                        <  2:2508047/140‑1 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGCGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:2208326/1‑140 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCCTCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:187801/1‑140 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCTTTTCGTTTTTTCATATAAGAACGGCGCAAGc                        >  2:284313/1‑140 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:5106094/1‑140 (MQ=255)
                                                                                                                     gTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCgt                      <  2:1370433/140‑1 (MQ=255)
                                                                                                                      tAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCgtg                     >  1:419656/1‑140 (MQ=255)
                                                                                                                              tttCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAATAACGGCGCAAGCGTGTCTGTAAt             >  1:243970/1‑140 (MQ=255)
                                                                                                                              tttCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAAt             >  2:3069965/1‑140 (MQ=255)
                                                                                                                                tcatcaTATTTAATTTCAATAAAAAAAGATGCACTCCAAAGTGAGTGAATGTTTTTTTTAAACTTCCATGATGATCGGAAGGTTGATCGTACTGCTTTTATTTTTTTGTTATAATAACGGCTCAAGCGTGTCttttatat           <  2:3495223/140‑8 (MQ=255)
                                                                                                                                tCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAAtct           >  2:1023494/1‑140 (MQ=255)
                                                                                                                                  aGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCa         >  2:4332626/1‑140 (MQ=255)
                                                                                                                                    cATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGAGTCTGTAATCTCAtt       >  1:3943717/1‑140 (MQ=255)
                                                                                                                                       attgcattgcAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCAtttt     >  2:4238655/1‑140 (MQ=255)
                                                                                                                                       attgcattgcAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCAttttt    >  2:1466108/1‑140 (MQ=255)
                                                                                                                                         tgcattgcaATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGa  >  1:3602132/1‑140 (MQ=255)
                                                                                                                                         tgcattgcaATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGa  >  1:2949965/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGA  >  NZ_CP034943/1488044‑1488320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: