Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,512,556 G→T 5.2% intergenic (‑84/‑29) EO946_RS07905 ← / → typA YlaF family protein/translational GTPase TypA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,512,5560GT 5.2% 721.8 / 7.1 251intergenic (‑84/‑29)EO946_RS07905/typAYlaF family protein/translational GTPase TypA
Reads supporting (aligned to +/- strand):  ref base G (160/69);  new base T (2/20);  total (162/89)
Fisher's exact test for biased strand distribution p-value = 2.52e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

TACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTGCCAAC  >  NZ_CP034943/1512418‑1512695
                                                                                                                                          |                                                                                                                                           
taCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGt                                                                                                                                            >  2:3173275/1‑140 (MQ=255)
   tGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACATAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATATTGTGAAAGttgt                                                                                                                                         >  2:2928625/1‑138 (MQ=255)
   tGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTAt                                                                                                                                         <  1:923333/140‑1 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATTTGAAAGTTATCt                                                                                                                                       >  1:4753448/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  2:2888490/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:2748846/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:2639571/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  2:3098884/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:255067/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:312017/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  2:4412228/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  2:2484260/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:3856291/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  2:4640371/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:1166922/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:627/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTGTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:4904486/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTAATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGATGATGTGTTGTATAATGTGAAAGTTATCt                                                                                                                                       >  1:1209894/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGACAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGACAGTTATCt                                                                                                                                       >  1:3255119/1‑140 (MQ=255)
     aaaaCCGCTGCAAATGCAAATAATAGTAAAAGACAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                                       <  2:5043512/140‑1 (MQ=255)
       aaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTaa                                                                                                                                     >  2:2638321/1‑140 (MQ=255)
          cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGATGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                                  >  2:3930284/1‑140 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGACAGTTATCTAATTtta                                                                                                                                >  1:4967618/1‑140 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAtt                                                                                                                                   <  2:3639956/137‑1 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAtt                                                                                                                                   >  1:4591285/1‑137 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAtt                                                                                                                                   >  1:3639956/1‑137 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTtta                                                                                                                                >  1:1812566/1‑140 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTtta                                                                                                                                >  1:4834127/1‑140 (MQ=255)
            cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTtta                                                                                                                                >  1:743936/1‑140 (MQ=255)
                       aataatAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAGTTTATTAGGAGATGa                                                                                                                     >  2:1179992/1‑140 (MQ=255)
                         taataGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGaaa                                                                                                                   >  1:2247264/1‑140 (MQ=255)
                         taataGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGaaa                                                                                                                   >  1:4926934/1‑140 (MQ=255)
                              gTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATGTTATTAGGAGATGAAAAAGTg                                                                                                              >  2:4196110/1‑140 (MQ=255)
                                    gCCAATTCATTTTTTTCATCTTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACtt                                                                                                        >  2:146137/1‑140 (MQ=255)
                                    gCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGAGGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACtt                                                                                                        >  1:5154372/1‑140 (MQ=255)
                                    gCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTCTAATGTGTAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAAGtt                                                                                                        >  1:502627/1‑140 (MQ=255)
                                         ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAATCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                                   >  1:848048/1‑140 (MQ=255)
                                         ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                                   >  1:3502423/1‑140 (MQ=255)
                                         ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                                   >  1:4498060/1‑140 (MQ=255)
                                           cATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                                 <  2:2231796/140‑1 (MQ=255)
                                           cATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                                 <  2:581773/140‑1 (MQ=255)
                                           cATTTTTTTCATATTTTCCATCCCCCCTAACCTCTGATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGACGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                                 <  1:2064485/140‑1 (MQ=255)
                                           aaTTTTTTTCATATTTTCCATCCCATTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                                 <  2:587042/139‑1 (MQ=255)
                                             tttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                               <  2:4515835/140‑1 (MQ=255)
                                             tttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                               <  2:4975168/140‑1 (MQ=255)
                                             tttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                               <  1:1148039/140‑1 (MQ=255)
                                              ttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGAAGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAATTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATc                                                                                              >  2:348407/1‑140 (MQ=255)
                                                           tCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATcgc                                                                                 <  1:3198328/140‑1 (MQ=255)
                                                           tCCATCCCCTTTTACGTATTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATcgc                                                                                 <  1:4325987/140‑1 (MQ=255)
                                                                  ccTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTACAGAGGATGTGTGGTATAATGTGAAAGTTATCTACTTTTAGTAGGAGAGGAAAAAGTGAAACTTCGAAATGATCTTCGCACCCTCGCGcttatt                                                                          >  1:214990/1‑140 (MQ=255)
                                                                   cTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTg                                                                         <  1:1075134/140‑1 (MQ=255)
                                                                   cTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTg                                                                         >  2:521046/1‑140 (MQ=255)
                                                                    ttttACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGc                                                                        <  1:4459711/140‑1 (MQ=255)
                                                                     tttACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGcc                                                                       <  1:2459233/140‑1 (MQ=255)
                                                                         cGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACg                                                                   <  1:4412228/140‑1 (MQ=255)
                                                                             ttATCATAATTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                               <  2:536321/140‑1 (MQ=255)
                                                                             ttATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATcgc                                                                                 >  1:3059673/1‑122 (MQ=255)
                                                                             ttATCATAAGTTTAAAGAAAAAAGTGACTCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGTAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                               <  2:2554950/140‑1 (MQ=255)
                                                                              tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                              >  2:138188/1‑140 (MQ=255)
                                                                              tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                              >  1:2283398/1‑140 (MQ=255)
                                                                              tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                              >  2:4437805/1‑140 (MQ=255)
                                                                              tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                              >  1:3697136/1‑140 (MQ=255)
                                                                              tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                              >  2:63251/1‑140 (MQ=255)
                                                                              tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAAGGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGACCACGTTGAc                                                              >  1:2619484/1‑140 (MQ=255)
                                                                                          aCAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACg                                                  >  2:3473374/1‑140 (MQ=255)
                                                                                          aCAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACg                                                  >  1:4139797/1‑140 (MQ=255)
                                                                                                 aaCGTGACGCTTTTAAAGAGGATGTGGGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAg                                           >  1:1452387/1‑140 (MQ=255)
                                                                                                       aCGCTTTTAACGAGTATGTTTGGTTTATTTTTAAATTTATATATTTTTATTCGGATATCAAAAAGTTAAACTTCGAAATCATCTTCCCAAAAGCGCTTTTTTTGCCCACTTTGACAAGGGGAAAACGACTCTAGTCGATc                                     <  1:3780313/140‑1 (MQ=255)
                                                                                                             ttAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCttt                               >  2:854701/1‑140 (MQ=255)
                                                                                                             ttAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCttt                               >  2:4604840/1‑140 (MQ=255)
                                                                                                             ttAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCttt                               >  2:1849332/1‑140 (MQ=255)
                                                                                                             ttAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCttt                               >  2:1576365/1‑140 (MQ=255)
                                                                                                                  ttggttgtttgGTATATTTTTAATTTTTTATATTTTTATTTGTATATTAAAAATTCAAAGTTCGAAATTATATTCGCAACATCGCGTTTATGGCCCACGTTCACCATGGGAAAACGACTCTAGTCGATCAGCTTTTacac                          <  2:5083444/135‑1 (MQ=255)
                                                                                                                  ttggATGGGTGGTATAATTTCAATGTTATCTAATTTTATTTGGAGAATAAAATGTAAAACTTCTAAATGATCTTCGAACCTTCGCTATTATGGCCCACGTTGACCAGGGGAACACGACTCTAGTCGATCAGCTTTTacac                          <  1:3415237/138‑1 (MQ=255)
                                                                                                                  tagGTTGTGTGTTTTAATTTTAATTTTATATAATTTTATTTGTATTTTAAAAATTGAAAGTTCGAAATTATCTTCGAAACATCGCTTTTTTGGCCCACTTTGACCATGGCAAAACGACTCTAGTCGATCAGCTTTTacac                          <  2:4834127/139‑1 (MQ=255)
                                                                                                                  gtgtttgtttgttATATTTTTAAAGTTATCTATTTTTATTAGGTGTTGAAAAATTGAAATTTCGAAAGTATCTTCGCAACTTCGGTTTTATTGCCACCGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTacac                          <  2:1117446/135‑1 (MQ=255)
                                                                                                                  gagGTTGTGTGTTTTAATGTTAAAGTTATATATTTTTTTTAGGTGATCAAAAATTCAAAGTTCTAATTGAGCTGCCCACCACCGCGTTTTTGGCCCAGGTTGACCTTGGCAACACGACTCTAGTCGATCAGCTTTTacac                          <  1:4159354/140‑1 (MQ=255)
                                                                                                                     gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg                       >  1:5121981/1‑140 (MQ=255)
                                                                                                                     gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg                       >  2:2420798/1‑140 (MQ=255)
                                                                                                                     gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg                       >  2:3663078/1‑140 (MQ=255)
                                                                                                                     gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg                       >  1:1742081/1‑140 (MQ=255)
                                                                                                                     gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGATTTTACACCAg                       >  2:2731490/1‑140 (MQ=255)
                                                                                                                     gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCACCTTTTACTCCAg                       >  2:4324544/1‑140 (MQ=255)
                                                                                                                      ttgtttgttATAATTTTAAAGTTAAATAATTTTATTAGGATATGAAAAAGTTAAACTTCCAAATTATCTTCGCAACTGCGCGTTTATGGCCCCCGTTCACCAGGGTAACACGACTCTAGTCGATCAGCTTTTACACCAgg                      <  1:3479487/139‑1 (MQ=255)
                                                                                                                      ttgtgtgTTATATTGTTAAATTTATATATTTTTATTTGGATATTAAAAATTCAAACTTCCAAATCATCTCCACAACCGCGCGTTTATGGCCCCGGTTCACCATGGCAACACGACTCTAGTCGATCAGCTTTTACACCAgg                      <  1:2781015/139‑1 (MQ=255)
                                                                                                                      ttgtgtgTTATACTGTGAATGTTTAATACTTTTATTAGGAGATGAAAAAGTCAAAGTTCGAAATGATCTTCGAAACATCGCGATTATTCCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAgg                      <  2:1252592/139‑1 (MQ=255)
                                                                                                                           tgttataTTGTGAAAGTTATCTATTTTTATTAGGATATCAAAAAGTTAAATTTCTAAATTATCTTCGCAACATCGCGTTTTTGGCCCAGGTTGACCTTGGTAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGt                 <  2:3856291/137‑1 (MQ=255)
                                                                                                                                   tgtgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCg         >  1:1972469/1‑140 (MQ=255)
                                                                                                                                   tgtgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCg         >  1:811583/1‑140 (MQ=255)
                                                                                                                                   tgtgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATAAGCTTTTACACCAGGCTGGTACGTTcct         >  1:1267986/1‑139 (MQ=255)
                                                                                                                                     tgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACTACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTg       >  1:5044414/1‑140 (MQ=255)
                                                                                                                                     tgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTg       >  1:1524990/1‑140 (MQ=255)
                                                                                                                                     tgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTg       >  1:2178013/1‑140 (MQ=255)
                                                                                                                                     tgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTg       >  1:1374460/1‑140 (MQ=255)
                                                                                                                                     tgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTg       >  1:5217033/1‑140 (MQ=255)
                                                                                                                                     tgAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACATTCCGTg       >  2:738121/1‑140 (MQ=255)
                                                                                                                                      taaaTTTATATATTTTAATTCGTAGATAAAAACGTAAAACTTCCAAATCATCTTCGCAACCCCGCGATTAGTGCCCAGGTGGACCATGGGAACACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTGc      <  2:2676278/139‑1 (MQ=255)
                                                                                                                                      taaaTTTAACTATTTTTATTAGTATATGAAAAAGTGAAACTTCTAATTTATCTTCGCAACATCGCGTTTCTTGCCCCGTTTGACCAGGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTGc      <  2:3396007/139‑1 (MQ=255)
                                                                                                                                      gAAATTTATATATTTTTATTAGTATATGAAAAAGTTAAACTTCGAAATTATCTCCGCAACAGCGCGATTATTGCCCACGTTGACCATGGGAACACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTGc      <  2:4294706/140‑1 (MQ=255)
                                                                                                                                          gTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTGCCAAc  >  2:2314817/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCCGTGCCAAC  >  NZ_CP034943/1512418‑1512695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: