BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,514,881	G→T	7.8%	Q62K (CAA→AAA)	EO946_RS07920 ←	YlaI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,514,881	0	G	T	7.8%	630.0 / 7.9	242	Q62K (CAA→AAA)	EO946_RS07920	YlaI family protein
Reads supporting (aligned to +/- strand): ref base G (47/166); new base T (26/3); total (73/169)
Fisher's exact test for biased strand distribution p-value = 1.31e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCA  >  NZ_CP034943/1514742‑1515020

cATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTGTGTTTTGTATGGTAACGCGGCCGCTTTATTTTTTGACATTTTCTTTTTTTTg                                                                                                                                              <  2:5194742/140‑1 (MQ=255)

  tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCt                                                                                                                                            >  2:1262754/1‑140 (MQ=255)

      cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGTCAGCTTTATTTTTTTAATTTTTCTTTTTTTTTCtttac                                                                                                                                        <  2:4458358/140‑5 (MQ=255)

      aaaaaTTCTGATTTATCAGTCGAAATATGTATATTATGCTAAATAATAATAAGACTGAGCTTATAAGCTGAGTCTTTTTTTGTTTTTTGTTTGGTAAAGCTTAATCTTTATTTTTTTACTTTTTGTTTTTTTTTCtttac                                                                                                                                        <  2:3885262/139‑5 (MQ=255)

         aaTTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTGTTTTGTGTTTTGTCTGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTGTTTTTTTCTGTCCCgg                                                                                                                                     >  1:588738/1‑140 (MQ=255)

              ggCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGaa                                                                                                                                <  2:1462375/140‑1 (MQ=255)

                  gTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGt                                                                                                                            <  1:4184009/140‑1 (MQ=255)

                  gTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTATTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGt                                                                                                                            <  1:2906524/140‑1 (MQ=255)

                         tCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAAtttt                                                                                                                     >  2:1701927/1‑140 (MQ=255)

                         tCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                                                    <  1:871778/140‑1 (MQ=255)

                          cGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                                                    <  2:3950989/140‑1 (MQ=255)

                          cGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                                                    <  2:4817742/140‑1 (MQ=255)

                           gAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTcc                                                                                                                   <  1:621340/140‑1 (MQ=255)

                                  ggACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGTTAAAGCGGCCGCTTTATTTTTTGACTGTTTCTTTTTTTTGGTGTCCCGGGTGAAAAGTGAATTTTGCCGtttta                                                                                                            >  2:3809992/1‑139 (MQ=255)

                                  ggACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCGtttta                                                                                                            >  2:3480420/1‑139 (MQ=255)

                                   gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTAAGTCTATTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTTAAAAGTGAATTTTCAAGTTTTCa                                                                                                           >  2:4141290/1‑140 (MQ=255)

                                    aCAGTCTGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAg                                                                                                          <  1:3231997/140‑1 (MQ=255)

                                       gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCg                                                                                                       >  2:3599130/1‑140 (MQ=255)

                                       gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCg                                                                                                       <  2:3709621/140‑1 (MQ=255)

                                       gTCAGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGGGTGTTGTATGGTAAACCGGCCGCTTTCTTTTTTGAGTTTTGCTTTTTTTTGCTGTCCCGGGTGAAAAGTTAATTTTCGCGTTGTCAGcgg                                                                                                       >  2:3387086/1‑138 (MQ=255)

                                        tCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTGTTTGTCTTTTTGTTTTTTTTTGTGTCCCGGGTGAAAAGTGTATTTTACCGGTTTGAGCCGt                                                                                                      >  1:2460427/1‑140 (MQ=255)

                                          tGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTTCTTTTT‑GTTTTTTTGCTGTACCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGtgcg                                                                                                   >  2:235921/1‑137 (MQ=255)

                                            cAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGttctt                                                                                                  <  2:4298519/140‑1 (MQ=255)

                                             aaaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGttcttc                                                                                                 <  1:3853648/140‑1 (MQ=255)

                                             aaaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGttcttc                                                                                                 <  1:585687/140‑1 (MQ=255)

                                                  cATAAAAAGACTCAGCTGAGAAGCTGAGTCGTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGTCTTTTTCTTTTTTTTGCTTTCCCGGGTGAAAAGTGAATTTTCCCGGTTTCAGCCGTTCTTCTt‑ttt                                                                                           >  2:1143434/1‑140 (MQ=255)

                                                   aTAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑tttt                                                                                          <  1:3599130/140‑1 (MQ=255)

                                                   aTAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑tttt                                                                                          <  2:2254408/140‑1 (MQ=255)

                                                   aTAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑tttt                                                                                          <  2:218554/140‑1 (MQ=255)

                                                        aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTGCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTggg                                                                                     <  1:4776478/140‑1 (MQ=255)

                                                        aaGACTCAGCGGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTGTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTggg                                                                                     <  2:1881294/140‑1 (MQ=255)

                                                           aCTCAGCTGAGAAGCTGAGTCTTTTTTTGGGTTTTGTATGGTAAAGCGGCCGGGTTTTGTTTTGTGTTTTTGTTTTTTTTTGTGTGCCGGGTGTAAAGTGAAATTTGCCGGTTTGAGCCGTTGTTATGTTTTTTTGGGCa                                                                                   >  1:544748/1‑140 (MQ=255)

                                                            cTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAAt                                                                                 <  2:4700425/140‑1 (MQ=255)

                                                             tCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGGGTTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTTCTG‑TTTTTTGTGCAATc                                                                                >  2:4422656/1‑140 (MQ=255)

                                                                 cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGt                                                                            <  2:4676471/140‑1 (MQ=255)

                                                                     gaAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGGTATTTTTTGACTGTTTGTTTTTTTTTGTGTCCCGGGTGGAAAGTGAATTTTACCGTTTTGAGCCGGGGTTGTG‑TTTTTTTGGGCATCCgggggtg                                                                        >  1:4276051/1‑135 (MQ=255)

                                                                       aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGc                                                                      <  2:1501341/140‑1 (MQ=255)

                                                                         cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGGGTTATTTTTTGAGTGTTTGTTTTTTTTGGTGTCCCGGGGTCAAACTGATGGTTCCCGGTTTGCGGCGTTGGTGTG‑GTTTTGGGGCAATAGGGtggtgggag                                                                    >  1:4134831/1‑137 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTGTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTc                                                                  <  1:1648470/140‑1 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTTTCTTTTTATTTTTTTTGATGTACCCCGGGAAAAATTAATTTTACCGCTTTAAGCCGGTGTTATT‑TTTTTTTTGCAAAACGGTCGTgggcttc                                                                  >  1:1311052/1‑135 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGAAGGTTTGTTTTTTTTTCTGTCCCGGGTGAAAAAATAATTTTACCGTTTTAATCCGGTTTTATT‑TTTTTTTTGCAATCCCGGCGTGGGAttg                                                                  >  1:3336182/1‑139 (MQ=255)

                                                                              tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                               <  2:1400412/140‑1 (MQ=255)

                                                                               gtttttttGTGTTGTGTAGGGTAACGCGGCCGCTTTCTTTTGTGACTTTGTCTTTGTTTGGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                              <  2:4236535/139‑1 (MQ=255)

                                                                               cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                              <  1:1701927/140‑1 (MQ=255)

                                                                                tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                             <  1:3155446/140‑1 (MQ=255)

                                                                                tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                             <  1:5058095/140‑1 (MQ=255)

                                                                                tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                             <  1:2006689/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  2:4217582/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  1:2888399/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  2:2553347/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  2:2503257/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  2:673682/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  1:4163684/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  1:1874479/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  1:1524722/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  1:1015985/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCGGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  2:5024470/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTATTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                            <  2:363791/140‑1 (MQ=255)

                                                                                  tttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcac                                                           <  1:4511772/140‑1 (MQ=255)

                                                                                    tttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                                                         <  2:331195/140‑1 (MQ=255)

                                                                                    gttGTGTTTTGTAGGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                                                         <  2:4625961/139‑1 (MQ=255)

                                                                                     ttGTGTTTTTTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                        <  2:2652239/140‑1 (MQ=255)

                                                                                     ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                        <  2:593902/140‑1 (MQ=255)

                                                                                     ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                        <  1:3074307/140‑1 (MQ=255)

                                                                                           tttGTATGGTAAAGCGCCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgta                                                  <  1:4684651/140‑1 (MQ=255)

                                                                                             tGTATGGTTAAGCGGCCGCTTTATTTTTTTAATTTTTGTTTTTTATTCTGTACCGGGTGAAAAGTAAAATTTACCGTTTTAATACCTTCCTATG‑TTTTTTTTGCAATACGAAAGTTGCAATCCATGCACaaataattatt                                                >  1:3672145/1‑134 (MQ=255)

                                                                                                    tAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATTTATGGATTggg                                         >  2:1533718/1‑140 (MQ=255)

                                                                                                    tAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg                                         <  1:4153540/140‑1 (MQ=255)

                                                                                                     aaaGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGc                                        <  2:784328/140‑1 (MQ=255)

                                                                                                      acgCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCg                                       <  2:3336182/138‑1 (MQ=255)

                                                                                                       aGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATACCGTCGTGGCATTACTGGCACATATATGTATGGATTGGGCgg                                      >  2:1105406/1‑140 (MQ=255)

                                                                                                       aGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGGTGTCCCGGGTGAAAAGTGAAATTTACCGTTTTAAGCCGTTCTTCTG‑TTTTTTTGGGAAACCGGTCGTGGCATTCCTGGCAACTATATGTATTGATTGGGCgg                                      >  2:5052471/1‑140 (MQ=255)

                                                                                                         cGGCCGCTTTATTTTTTGAGTTTTTCTTTTTTTTTATTTACCGGGTGAAAAGTGAAATTTACAGTTTAAACCCCTTCTTCTT‑TTTTTTTGGCAATCGCGTAGTGGAAATCATGGCACATAAATGTATGTATTTGGCGGtt                                    >  2:4723698/1‑140 (MQ=255)

                                                                                                         cGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTACCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGtt                                    >  2:4550914/1‑140 (MQ=255)

                                                                                                              gCTTTATTTTTTGACTTTTTCTTTTTTTTGCTTTCCCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTTCTG‑TTTTTTTGGCAATCCGGTAGTGGCATTCCTGGCACATATTTGTATGGAATGGGCGGTTTCGaa                               >  2:1211050/1‑140 (MQ=255)

                                                                                                              gCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGGTCTTGTG‑TTTTTTGGGGCATCCGGTGGTGGCCTTCCGGGCCCAGAGAGGGAGGGTTTGGGCGGGTTCgga                               >  2:2900858/1‑138 (MQ=255)

                                                                                                                     tttttGACTTTTTCTTTTTTTTGCTGTCCCGGGTGCAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGttt                        <  1:321792/140‑1 (MQ=255)

                                                                                                                      ttttGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTc                       <  1:4856293/140‑1 (MQ=255)

                                                                                                                      tattGACTTTTTATTTTTTTTGCGGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTCTGGATTGGGCGGTTTCGACGCCGTTTc                       <  1:5143261/138‑1 (MQ=255)

                                                                                                                         tGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTcgcg                    >  1:710869/1‑140 (MQ=255)

                                                                                                                          gACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGa                   >  1:1165511/1‑140 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  2:892816/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:19921/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  2:2420907/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:3418083/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  2:1347884/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:988316/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  2:4953585/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  2:4905034/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGGATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:402907/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTTTTGTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:2283376/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCTTTGTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  2:4324593/140‑1 (MQ=255)

                                                                                                                           aCTTTTTCGTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTATTCTG‑TTTTTTGTGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:3719984/140‑1 (MQ=255)

                                                                                                                           aCTTGTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                  <  1:4718761/140‑1 (MQ=255)

                                                                                                                            ctttttctttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATc                 <  2:3157446/140‑1 (MQ=255)

                                                                                                                            ctttttctttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATc                 <  2:3694139/140‑1 (MQ=255)

                                                                                                                            ctttttctttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATc                 <  2:4022176/140‑1 (MQ=255)

                                                                                                                                       ttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatcatc      <  2:1677769/140‑1 (MQ=255)

                                                                                                                                       ttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatcatc      <  2:1982031/140‑1 (MQ=255)

                                                                                                                                           gCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTAtcatcatca  <  1:3014261/140‑1 (MQ=255)

CATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTG‑TTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCA  >  NZ_CP034943/1514742‑1515020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`