Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,582,922 C→A 7.2% A38E (GCA→GAA)  ylyA → sporulation‑related RNA polymerase‑binding protein YlyA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,582,9220CA 7.2% 617.9 / 6.2 245A38E (GCA→GAA) ylyAsporulation‑related RNA polymerase‑binding protein YlyA
Reads supporting (aligned to +/- strand):  ref base C (134/84);  new base A (2/25);  total (136/109)
Fisher's exact test for biased strand distribution p-value = 7.54e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

CTACGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAG  >  NZ_CP034943/1582783‑1583061
                                                                                                                                           |                                                                                                                                           
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACACGCCCGCAcgcagc                                                                                                                                             >  2:3169328/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:4316196/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:2428154/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:2633351/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:1494252/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:1170930/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:1002696/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:3906731/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:3959155/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:4661845/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:4918272/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  1:2584565/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:76452/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  1:1376618/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  1:1461922/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  1:1484949/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  1:2050232/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGATTTCAGGCTTCAACAAGCCCGCAagcagc                                                                                                                                             >  2:4387886/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTAGAGATTATTTGAGTATTCTTGATTTCAGGCTTAAACAATCCCGCAagcagc                                                                                                                                             >  2:2972754/1‑140 (MQ=255)
ctacGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATAGATACGGAACTTCTTCTGACGACAGAAGAATTACAGGCGAGAGTCTTTGAGTATTCTTGCGTTCAGGCTTCAACAAGCCCGCAcccagc                                                                                                                                             >  2:1061112/1‑140 (MQ=255)
  acGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGcaa                                                                                                                                           >  1:2359543/1‑140 (MQ=255)
  acGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGcaa                                                                                                                                           >  1:1106425/1‑140 (MQ=255)
  acGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGcaa                                                                                                                                           >  1:900098/1‑140 (MQ=255)
  acGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTATTGCTTTCAGGCTTCAACAAGCCCGCAAGCACcaa                                                                                                                                           >  2:2673337/1‑140 (MQ=255)
   cGTTAAGAGCAGGAGTTGTGATGACTGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGcaat                                                                                                                                          <  1:4291852/140‑1 (MQ=255)
     ttAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGcaatca                                                                                                                                        >  2:688379/1‑140 (MQ=255)
        agagCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCaatcaatc                                                                                                                                     >  1:1788341/1‑140 (MQ=255)
          agCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCAt                                                                                                                                   <  1:3027101/140‑1 (MQ=255)
             aGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAGGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTAAGGCTTCAAAAAGCCCGCAAGCAGCAATCAATCATaaa                                                                                                                                >  2:2923710/1‑140 (MQ=255)
               gaattGTGATGAATGAATGATCAACTGCCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACa                                                                                                                              <  2:2095032/137‑1 (MQ=255)
               gAGTTGTTATTAATGAATGATAAACTGACCGAAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGATATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACa                                                                                                                              <  2:3719347/140‑1 (MQ=255)
               gAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACa                                                                                                                              >  2:3593256/1‑140 (MQ=255)
                    gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAg                                                                                                                         >  1:1930875/1‑140 (MQ=255)
                          aatgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAActc                                                                                                                   >  1:3364705/1‑140 (MQ=255)
                            tgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTg                                                                                                                 >  1:3933093/1‑140 (MQ=255)
                               atgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATc                                                                                                              >  1:4781020/1‑140 (MQ=255)
                                  ataaaCTGACCGCAATATATACGTAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                           <  2:3030817/137‑1 (MQ=255)
                                  aTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                           >  2:2194661/1‑140 (MQ=255)
                                  aTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                           <  2:4114657/140‑1 (MQ=255)
                                  aTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                           <  2:4236386/140‑1 (MQ=255)
                                  aTCAACTGACCGAAATATATACGTAACTTCTTCTGACGAAAGAATAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                           <  2:4041105/140‑1 (MQ=255)
                                    cAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATca                                                                                                         >  1:629582/1‑140 (MQ=255)
                                    cAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATca                                                                                                         >  2:3308950/1‑140 (MQ=255)
                                    aaaCTGACCGCAATATATACTGACCTTCTGCTGACTAAATAACAATTAGATTCGATTTTATTTTTTTATTGTTGTTTCCCGGCTTCAACAACCCCGAAAGCAGCAATAAATCATAAAAAAAAAGCAACTCTGATCTATca                                                                                                         <  1:4719968/139‑1 (MQ=255)
                                      aCTGACCTAAATATATCCGCAACTTATTCTGACTAAATAAGAATTAAAGTCTAGTTTTTTTGTTTTTTGTTGATTTCAGGCTAAAACAACCCCGCAAGCAGCAATCAATAATAAAAAAAACGCAACTCTGATCTATcaca                                                                                                       <  1:4108951/140‑1 (MQ=255)
                                          aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCAGAACTATCACATTaa                                                                                                   >  1:2468649/1‑140 (MQ=255)
                                              cAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATAAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                               <  2:2555815/140‑1 (MQ=255)
                                              cAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGAAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                               <  2:5187015/140‑1 (MQ=255)
                                              aaaTATATACGTAACTTCTTCTGACGAAATAATAATTACAGTCGATTTTATTTGATTATTCTTGCTTTCAGGCTTAAACAAGCCCGAAAGCAGAAATAAATAATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                               <  2:1433467/139‑1 (MQ=255)
                                                 tatataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAagag                                                                                            >  1:2246161/1‑140 (MQ=255)
                                                    ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                         >  1:354251/1‑140 (MQ=255)
                                                      aCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCa                                                                                       >  1:3180464/1‑140 (MQ=255)
                                                      aCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCa                                                                                       >  1:3447471/1‑140 (MQ=255)
                                                      aCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCa                                                                                       >  1:2840603/1‑140 (MQ=255)
                                                        tgAACTACTTCTGACTAAAGAATAATTACAGTCCATATTATTTGATTATTCTTCATTACAGGCTTCAACACCCCCGAAAGCAGCAAACAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAg                                                                                     <  1:5177145/139‑1 (MQ=255)
                                                         gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGTCTTCAACAAGCCCGCAAGCAGAAATCAATCATAAACAAAAAGCAACTCGTATCTATCACATTAAAGAAGAGCTTAAAGa                                                                                    >  1:1659580/1‑140 (MQ=255)
                                                         gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                    >  2:175850/1‑140 (MQ=255)
                                                         gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                    >  1:3215651/1‑140 (MQ=255)
                                                           aCTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACg                                                                                  >  1:4161959/1‑140 (MQ=255)
                                                           aCTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACg                                                                                  >  1:395333/1‑140 (MQ=255)
                                                            cttcttCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGAAATCAAACATAAACAACAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGt                                                                                 <  2:1623375/140‑1 (MQ=255)
                                                            attcttaTGACGAAAGAAGAATTACAGTGGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCACGAAAGAAGAAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGt                                                                                 <  2:2703361/139‑1 (MQ=255)
                                                             ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                >  2:865696/1‑140 (MQ=255)
                                                             ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                >  2:4528449/1‑140 (MQ=255)
                                                             ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                >  2:169144/1‑140 (MQ=255)
                                                             ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                >  1:3855040/1‑140 (MQ=255)
                                                             ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                >  1:511866/1‑140 (MQ=255)
                                                                    gACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCg                                                                         >  2:3384284/1‑140 (MQ=255)
                                                                    gACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTATCCTCg                                                                         >  2:1123835/1‑140 (MQ=255)
                                                                      ccaaaCAAAAAATAAAGTCTACATTATTTCAGTATTCTTCTTTTACGCCTAAAAAAACCCCCCACGCAGAAATAAATCAAAAACAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                       <  1:2647176/138‑1 (MQ=255)
                                                                                  tACAGTCTATATTATTTGATTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATc                                                           <  2:641979/140‑1 (MQ=255)
                                                                                         gagaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACg                                                    >  1:5189831/1‑140 (MQ=255)
                                                                                          agaTTTTTTGAGTTTTGTTGCTTTCAGGCTTCAACAAGCCCGCACGCAGAAATCAACCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACgg                                                   <  2:1968777/140‑1 (MQ=255)
                                                                                          agaTTATTTGATTATTCTTGATTTCAGGCTTCAACAAGCCCGCAAGAAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACgg                                                   <  2:3502875/140‑1 (MQ=255)
                                                                                          agaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACgg                                                   <  2:124535/140‑1 (MQ=255)
                                                                                            tttattTGATTTTTCTTGCTTTCAGGCTTAAACACGCCCGCAAGCAGCAATCAATCATAAAAAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 <  1:4661845/139‑1 (MQ=255)
                                                                                            atttttTGAGTATTCTTGCTTTCAGGCTTCAACACGCCCGCACGCAGAAATCAAACATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 <  1:4591932/140‑1 (MQ=255)
                                                                                            attattTGATTATTCTTGCTTTCAGGCTTCACCAAGCCCGCAAGCAGCAATCAATAAAAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 <  1:4020440/140‑1 (MQ=255)
                                                                                            attattTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 >  2:3620772/1‑140 (MQ=255)
                                                                                            attattTGAGTATTCTTGCTTTCAGGCTACAACAAGCCCGCACGCAGCAATAAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 <  1:5063279/140‑1 (MQ=255)
                                                                                            attattTGAGTATTATTGCTTTCCGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 <  1:1028896/140‑1 (MQ=255)
                                                                                            attaatTGATTATTGGTGCTTTCAGGCTACCACACCCCCGCACGCAGCAATCACTCATAAACAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                 <  1:4989931/140‑1 (MQ=255)
                                                                                                     gTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGAt                                        >  2:1517983/1‑140 (MQ=255)
                                                                                                       aTTCTTGCTTTCAGCCTCAAAAAAGCCCCCACGCACCAAACAATCATAAACAAAAACCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATAc                                      <  1:69806/140‑1 (MQ=255)
                                                                                                                 tccggCTTCAACAAGCCCGCAAGCAGCCATCAAACATAAACAAAAAGCAACTCTGATCTATCACATTAAAGCAGAGCTTCAAGACGTTCTCCACGCAGTGTCCAAAATCGAAACCGGTACATTCGGGTACTGCGAAGaaa                            <  1:2633351/137‑1 (MQ=255)
                                                                                                                     gCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgg                        <  2:3786283/140‑1 (MQ=255)
                                                                                                                     gCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgg                        >  2:2841229/1‑140 (MQ=255)
                                                                                                                       ttaaacaaGCCCGAAAGCAGAAATCAATAATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGTTAACTTTAAATTCGTATTCTGGGTAGATACttttg                      <  1:76452/137‑5 (MQ=255)
                                                                                                                       ttCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                      <  1:1002696/140‑1 (MQ=255)
                                                                                                                       tacaacacGCCCGAAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                      <  1:4918724/138‑1 (MQ=255)
                                                                                                                        tCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGc                     <  2:1376618/140‑1 (MQ=255)
                                                                                                                               gCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAATAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCAtt              >  1:361753/1‑140 (MQ=255)
                                                                                                                                 ccGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTcc            <  2:5082327/140‑1 (MQ=255)
                                                                                                                                 ccGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTcc            <  2:3215651/140‑1 (MQ=255)
                                                                                                                                       caagcaATCAATCATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTc      <  1:807161/138‑1 (MQ=255)
                                                                                                                                         agcaATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaa    >  2:4927057/1‑140 (MQ=255)
                                                                                                                                          gcaATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaaa   <  2:5042457/140‑1 (MQ=255)
                                                                                                                                          gcaATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaaa   <  2:558542/140‑1 (MQ=255)
                                                                                                                                          gcaATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATCCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaaa   >  2:2335629/1‑140 (MQ=255)
                                                                                                                                           caatcaatCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGTAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATAGTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaatg  >  1:2757419/1‑138 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTACGTTAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAG  >  NZ_CP034943/1582783‑1583061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: