Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,800,874 A→C 15.0% S3519R (AGC→CGC)  EO946_RS09135 → SDR family NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,800,8740AC15.0% 544.4 / 34.4 270S3519R (AGC→CGC) EO946_RS09135SDR family NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (138/82);  new base C (2/48);  total (140/130)
Fisher's exact test for biased strand distribution p-value = 1.17e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

ATTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAACGCCG  >  NZ_CP034943/1800738‑1801013
                                                                                                                                        |                                                                                                                                           
    aTCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCg                                                                                                                                      >  2:4962439/1‑140 (MQ=255)
     tggttttctttttAACGGAACACAAAGAGATTATCTAAGCATTTTCCCGGGTTTATGGGTGTGAGTCACAAAAGCCCCCTGAAGCTGTTTTGGAATTTCCTGCATCTATCTTACCACAAAAAAATAATATAAGCGACCgg                                                                                                                                     <  1:870204/135‑1 (MQ=255)
     tgggCTTCTTCTTAACGGATAACAAATAGCTTATCGAAGAATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCgg                                                                                                                                     <  2:5067115/138‑1 (MQ=255)
     tCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCgg                                                                                                                                     <  2:3896137/140‑1 (MQ=255)
     tCGGCTTCTTATTAACGGATCACAATGAGCTGATCGAAGAATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGATCTCGTACCATAAAACAATAATATAAGCGACCgg                                                                                                                                     <  2:3166610/140‑1 (MQ=255)
         cttcttctgAACGGATCACAACGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGACATTCCTGCAGCTCTCGTCCGAGACAACAATAATATAAGCGACCGGCAAt                                                                                                                                 >  1:4273933/1‑140 (MQ=255)
            cttcttAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAAtctc                                                                                                                              >  1:3583276/1‑140 (MQ=255)
                   aCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGa                                                                                                                       >  2:72376/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  1:801189/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  2:925096/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  2:4331409/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  2:3942710/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  2:2657932/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  2:1493926/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  1:4736962/1‑140 (MQ=255)
                            aaaGCGCTTCTCGAAGCTTTTTACCGGGTTGATGCGTCTGAGCCAAAAAAGCCCCCTTACGGTTATTTGCAAATGCCGGCAGCTCTCGTACAACAAAACAAAAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                              <  1:2271491/140‑1 (MQ=255)
                                cgCTGATCGAAGCTTTTTACGGGTATGAGGCTTCTGAGGCAAACAACCCCCCTGAAGCTTTTTTCTAACTTCCTGCAGCTCTCGAACCAGAAAACATTAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCatt                                                                                                          <  1:318067/139‑1 (MQ=255)
                                 tcTGATCTAACCATTTTCCCGGGTTGATGCTTCTCCGCCAAAAAACCCCCCTCAAGGTGATATGAAAATTCCTGCAGCTCTCTTACCAAAAAAAAATAATACAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         <  1:693902/139‑1 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGGTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  1:3116424/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCGatta                                                                                                         >  2:3105264/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  1:3590806/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  1:1211744/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  2:2542995/1‑140 (MQ=255)
                                  cTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCattat                                                                                                        >  2:3592412/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:2017794/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:208606/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:1039923/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:3749792/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCAGTATTgg                                                                                                     >  1:4142346/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACAAGAAAACAATAATATAAGCGACCGGCAATATCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:5203396/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGAGATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGTGCCTAGCGCCAGTCTTgg                                                                                                     >  2:3356882/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGACGCACAAAAGCCCCCTCAACCTGATATGGACATTCCAGCAGCTCTCCTACCAGAAAACAATAATATAAGCGACCCGCACTCTCACGCCGAGCCTATCGCCATTAGTgg                                                                                                     >  2:4452806/1‑140 (MQ=255)
                                      tCGACGAATTTTACCGGTATGATGCTTATGCGGAAAAAAAGCCCCCTGAAGCTGATATGTAAATTCCTGCAGCTCTCGTACCACAAAAAAATAATAAAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGc                                                                                                    <  1:2337035/140‑1 (MQ=255)
                                      tCGAAGCATTTTACCGGGATGATGCGTCTGAGGCAAATATCCCCCCTGAAGCTGATAAGGTAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGc                                                                                                    <  2:3015760/140‑1 (MQ=255)
                                       ctaaGAATTTTCCCGGTATGATGCGTCTGCGCCACAAAACCCCCCTCACGGTGATATGCAAATTCCGGCAGCTCTCGTACAACAAAACAATAATAACCGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCa                                                                                                   <  1:4348741/138‑1 (MQ=255)
                                         aaGCATTTTACCGGGATGATGCGTCTGAGGAACACAAGCCCCCTGACGCTGACATGGAAATTCCTGCAGCTCGCGTACCAGAACCCAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATg                                                                                                 <  2:2216941/140‑1 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  1:1506930/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  1:1484578/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCCCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  1:4867028/1‑140 (MQ=255)
                                                  aCCGGTATGATGCGTGTGCGGCACACACGCCCCCTGACGCTGATATGCAATTTCCGGCACCTCTCGTACCCCAAAACAATAATATCAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAgg                                                                                        <  1:1094147/140‑1 (MQ=255)
                                                   ccGGGATGAGGCTTCTGAGCCACAAACGCCCCCTGACGCTTATTTGGAAAGTCCGGCAGCGCGCGTACCAGAAAACAAAAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGt                                                                                       <  1:2083825/140‑1 (MQ=255)
                                                      ggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCGATTCTTGGCATGAGTGGCAGGTTTc                                                                                    >  1:2634585/1‑140 (MQ=255)
                                                      ggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTc                                                                                    >  1:5074542/1‑140 (MQ=255)
                                                      ggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTc                                                                                    >  1:270806/1‑140 (MQ=255)
                                                        ttgttgCGTCTGAGCCAAAAACGCCCCCTCAAGCTTATATGTAAATTCCTGCCGCTCTCTTACCACAAAACAATAATACACGCGACCGGCAATCTCCCGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:471118/136‑1 (MQ=255)
                                                        ttgatgCGTCTGAGGCAAAAAAGCCCCCTCAAGCTTATATGCAATTTCCGGCAGCTCTCGTACCACAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:3353463/139‑1 (MQ=255)
                                                        atgatgCTTCTGAGCAAAAAAACCCCCCTCAAGCTGATATGCAAATTCCTCCAGCTCTCGAACCATAAAAAAATAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:430004/140‑1 (MQ=255)
                                                        atgatgCGTGTGAGGCAAAAAAGCCCCCTTAAGCTTATTTGTAAATTCCTGCAGCTCTCTTACCACAAAACAATAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCTCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:435870/140‑1 (MQ=255)
                                                        atgatgCGTCTGATGCAAAAAAGCCCCCTTAAGCTTATATGGAAATTCCTGCAGCTCTCTTACCCCAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  2:4142346/140‑1 (MQ=255)
                                                        atgatgCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  2:4340412/140‑1 (MQ=255)
                                                        atgatgCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGAACCACAAAACAATAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:3398676/140‑1 (MQ=255)
                                                            tccGTCTCAGCCACAAAACCCCCCTCACGCTGATATGGAAATTCCGGCAGCTCTCGTCCCACAACACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAg                                                                              <  1:2766210/138‑1 (MQ=255)
                                                            ggCGTGTGAGGCACAAAACCCCCCGCACGGTTATTTGGAAATTCCTGCAGCTCTCGTACCACAAAAAAATACCAACAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAg                                                                              <  1:500867/139‑1 (MQ=255)
                                                              cGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCt                                                                            >  1:3465190/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                          >  1:1445047/1‑140 (MQ=255)
                                                                  tgcggCAAAAAACCCCCGTGACGGTGTTTTGGAATTTCGTGCAGCTCTCGTACCATAAAACAATATTAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                        <  1:1263218/137‑1 (MQ=255)
                                                                  tGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                        <  2:1493952/140‑1 (MQ=255)
                                                                  tGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                        <  2:1580117/140‑1 (MQ=255)
                                                                  tGAGCCACAAACGCCCCCTGACGCTTATATGGAAATTCCTGCAGCTCTGGTACCAGAAAAAAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                        <  2:148575/140‑1 (MQ=255)
                                                                   gAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATACGCGACCGGCAATCTCCCGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                       <  1:5209238/140‑1 (MQ=255)
                                                                   gAGGCACAAAAGCCCCCTGAAGCTGATATGCAAATTCCTGCAGCTCTCGTACCACAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                       <  1:275091/140‑1 (MQ=255)
                                                                   gAGCCACAAAAGCCCCCTGAAGCTGATATGCAATTTCCTGCAGCTCTCGTCCCAGAAAACAATAATCTCAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                       <  1:1160103/140‑1 (MQ=255)
                                                                    aGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCg                                                                      >  1:4070149/1‑140 (MQ=255)
                                                                    aGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCg                                                                      >  1:5166351/1‑140 (MQ=255)
                                                                    aGGCACAAAAGCCCCCTGAAGCTGATATGCAAATTCCTGCAGATATCGTCCCAGAAAAAATGAAAATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCg                                                                      <  2:1216797/140‑1 (MQ=255)
                                                                     ggCACAAAAGCCCCCTGAAGCTTATATGGAAATTCCTGCAGCTCTCGTACCAGAACACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCgg                                                                     <  2:2770261/140‑1 (MQ=255)
                                                                     ggCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCgg                                                                     >  1:166088/1‑140 (MQ=255)
                                                                         caAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCCGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCa                                                                 <  2:4894162/140‑1 (MQ=255)
                                                                         caAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAACACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCAGTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCa                                                                 >  2:49300/1‑140 (MQ=255)
                                                                         caAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCa                                                                 >  2:888526/1‑140 (MQ=255)
                                                                         caAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCa                                                                 >  1:4653010/1‑140 (MQ=255)
                                                                          aaaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAt                                                                >  2:3983484/1‑140 (MQ=255)
                                                                          aaaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAt                                                                >  2:3623141/1‑140 (MQ=255)
                                                                              gCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAGGAGc                                                            >  2:4752405/1‑140 (MQ=255)
                                                                               cccccTTAAGCTGATATGGAATTGCCTGCCGCTCTCGTACCACAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCt                                                           <  1:1248775/140‑1 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCa                                                                                                   >  1:2388524/1‑98 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCa                                                                                                   <  2:2388524/98‑1 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTAt                                                         >  1:3895221/1‑140 (MQ=255)
                                                                                           gATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCt                                               >  2:4711188/1‑140 (MQ=255)
                                                                                            atatGGAAATTGCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCACTCTCACGCCGAGCCTATCGCCATGATTGGCATGAGTGGCAGGTGTCCTCAAGCTGATTCGGTGCATGAGCTATGGGAGAATCTg                                              >  1:3014146/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGTCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  1:185250/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  2:797096/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  2:27460/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  2:110603/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  1:1329133/1‑140 (MQ=255)
                                                                                                ggAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGTAATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGaaaa                                          >  2:3538643/1‑140 (MQ=255)
                                                                                                   aaTTCCTGCAGCTCTCGTACCATAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACg                                       <  2:3292557/140‑1 (MQ=255)
                                                                                                    aTTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACgg                                      <  2:1445047/140‑1 (MQ=255)
                                                                                                      tCCTGCAGCTCTCTTACCAGAAAACAATACTATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCa                                    <  2:643414/140‑1 (MQ=255)
                                                                                                            aGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACAtt                              <  1:1493926/140‑1 (MQ=255)
                                                                                                                   gTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTAtctc                       >  2:1839374/1‑140 (MQ=255)
                                                                                                                      caaGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGa                    <  1:2945191/138‑1 (MQ=255)
                                                                                                                       cacaaaaCAATACCATCCGCGACCGGCACTCTCACCCCGAGCCTATCGCCAGTATTGGCATGAGTGGCAGGGTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGAc                   <  1:3975309/137‑1 (MQ=255)
                                                                                                                        aGAAAAAAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  1:611460/95‑1 (MQ=255)
                                                                                                                           aaaCAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATcc               >  2:4519367/1‑140 (MQ=255)
                                                                                                                                      taAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgc    <  2:3465190/140‑1 (MQ=255)
                                                                                                                                      taAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgc    <  1:2542939/140‑1 (MQ=255)
                                                                                                                                      taAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgc    <  1:1039923/140‑1 (MQ=255)
                                                                                                                                      taAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgc    <  1:3265909/140‑1 (MQ=255)
                                                                                                                                        aGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgccg  >  2:305838/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ATTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAACGCCG  >  NZ_CP034943/1800738‑1801013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: