Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,936,593 G→T 14.8% P3553Q (CCA→CAA)  EO946_RS09735 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,936,5930GT14.8% 733.0 / 39.2 375P3553Q (CCA→CAA) EO946_RS09735non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (127/181);  new base T (65/2);  total (192/183)
Fisher's exact test for biased strand distribution p-value = 3.02e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

CAAGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCT  >  NZ_CP034943/1936458‑1936731
                                                                                                                                       |                                                                                                                                          
cAAGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAg                                                                                                                                        >  2:5133695/1‑140 (MQ=255)
     aTCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCct                                                                                                                                   <  1:429771/140‑1 (MQ=255)
        cGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACTATTTTCGGTTGGTACTTAGACATTTTTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCtctc                                                                                                                                <  1:5050907/140‑1 (MQ=255)
               aCTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCTATCCTCCaaa                                                                                                                         >  2:3843456/1‑139 (MQ=255)
                  tCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTaa                                                                                                                      <  1:955185/140‑1 (MQ=255)
                      tGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGa                                                                                                                  <  1:2403883/140‑1 (MQ=255)
                         tGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGa                                                                                                               <  2:3897913/140‑1 (MQ=255)
                         tGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGa                                                                                                               <  2:3023833/140‑1 (MQ=255)
                           aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                             <  1:4623440/140‑1 (MQ=255)
                           aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGCGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                             <  2:4476869/140‑1 (MQ=255)
                                 gACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCgg                                                                                                       <  1:4131688/140‑1 (MQ=255)
                                  aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGt                                                                                                      <  2:4151087/140‑1 (MQ=255)
                                     cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCGCTCCTCCCATTAACAGATGTCGAAGCGGTACt                                                                                                   >  1:4534916/1‑140 (MQ=255)
                                          acacAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTTCCTCTCATCCAAATAAAACATGAAGAAACGGTaatacatg                                                                                              >  1:2680528/1‑133 (MQ=255)
                                          acacAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTCCTCCAATTAAAACATGACGAACCGGTAATCCTTc                                                                                              >  1:4334191/1‑140 (MQ=255)
                                          acacAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTTTAGTGCCTCTCCGCCAAATAAAAGATGAAGAAGCGGTACTCCGTc                                                                                              >  1:1768121/1‑140 (MQ=255)
                                           cacaAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGATCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcc                                                                                             <  2:2276084/140‑1 (MQ=255)
                                           cacaAGTGTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcc                                                                                             <  2:4229548/140‑1 (MQ=255)
                                           aaaaattttCGGTTGGTCCATATACATTTGTGCTGGCAGGTGCTGCCCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACGGTCGTTTTTGGAAGTGCCTCTCCTCAAATTAACAGATGACGAAGAGGTACTCCTTcc                                                                                             <  2:2931518/134‑1 (MQ=255)
                                                   tCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTGGCGCTCCTCCAATTAAAAGATGACGAAGCGGTACTCCTTGCCCatcatc                                                                                     >  1:1476359/1‑140 (MQ=255)
                                                          gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTACTCCAAATAACATATTAAGAAGCGGTAATCCGTACCCAACAAATGCAGCt                                                                              >  1:3027990/1‑140 (MQ=255)
                                                          gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGCCTCTCCTCCAAATAACAGATGAAGAAGCGGTACTCCTTCCCCATCATCTTCAGca                                                                              >  1:3536848/1‑139 (MQ=255)
                                                          gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGCCTCTCCGCCAAATAACATATTAAGTAGCGGTACTCCATCCCCATCCTCATCAGca                                                                              >  2:4724227/1‑139 (MQ=255)
                                                           tCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGGATTGCCGCTCCTCCAAATAAAAGATGACGAAGCGGTACTCCTTACCCATAATATGCAGCTa                                                                             >  1:4016581/1‑140 (MQ=255)
                                                           tCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCTCCAATTAAAAGATGACGAAGCCGTACTCCATCCCCAACATCTGCaccga                                                                             >  2:2826479/1‑136 (MQ=255)
                                                                ataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcagc                                                                        <  2:1107529/140‑1 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGGAGTTCCTCTCCTCCAATTAAAACATGACGAAGCGGTTCTCCTTCCCCATCATCTGGAGCTACCagcagcgt                                                                   >  2:2394999/1‑138 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTAGCCTCCAATTAAAACAAGACCAAGCGGGACTCCGTACCCATCAAAAGCAGCTACCAGCAGCtt                                                                   >  1:3451142/1‑140 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCTCGCCGCCCAATAAAAGATTACGAAGCGGTAATCCTTCCCCCTCAACTGCAGCGACCCGCAGCtt                                                                   >  1:1248308/1‑140 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATAATCTGCAGCTACCAGCAGCtt                                                                   >  2:2359148/1‑140 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCGCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCAGCATCTGCAGCTACCCGCCGCtt                                                                   >  2:142795/1‑140 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTACTCCAAGTAAAAGATGAACCAACGGTGCTCCTTACCCAACATCTGCAGCTCACagcagcgt                                                                   >  2:2690264/1‑138 (MQ=255)
                                                                     tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGGAGTGCCACGCCTCGAATTAACAGATTAAGACGCCCTACTCCTTCCCCAACAACTGCACCTACCCGCACCtt                                                                   >  1:4230415/1‑140 (MQ=255)
                                                                      ttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCGCCAAATAACATATGACGAAGCGGTACTCCGTCCCCCACATCTTCAGCGACCAGCAGCGTc                                                                  >  2:1281957/1‑140 (MQ=255)
                                                                       tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTTCCTCTCCGCCAATTAACAGATGACGAAGCGGTACTCCGTCCCCCTCATCTGCAGCTACCCGCCGCGTCa                                                                 >  1:2433445/1‑140 (MQ=255)
                                                                       tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCGGCTCCACTTAACGGATGGCGTAGCGGGACTCCGTCCCCCTCCTCTGGAGCTCCCcgcagcgtgc                                                                 >  2:1988434/1‑136 (MQ=255)
                                                                        gtgATGGCAGGTGCTGCTCTATCCGCGCCAAATCAGTGTCTGAACTTGGTTACCGTCGTTATTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCaa                                                                <  1:766955/140‑1 (MQ=255)
                                                                         tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCCATTAAAAGATTACGAAGCGGTACTCCTTACCCCTCATATGCAGCTACCAGCAGGATCaaa                                                               >  2:1867831/1‑140 (MQ=255)
                                                                         tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACATATGACGAAGCGGTACTCCTTCCCCATCAAATGCAGCTACCACCAGCTTaaaa                                                               >  2:1125099/1‑140 (MQ=255)
                                                                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCGCGGCTGGAATTAAAACATTACGAATCGGTACAACTTGCCCAACAAATGCATCTACCAGCAGCTTGGAATgggc                                                          >  1:1668102/1‑140 (MQ=255)
                                                                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTGTTGGAAGTGCCGGTGCGCCCATTAACAGATGTCGAAGCGGTACAGCTTCACCATCATCTGCAGCTAACAGCAGCTTAAAATgtgc                                                          >  1:1413286/1‑140 (MQ=255)
                                                                              gCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCGCCAATTAAAAGATGACGAAGCGGTACTCCTTACCCAACATCTGCAGCTACCAGCAGCTTGAAAAgggc                                                          >  2:37549/1‑140 (MQ=255)
                                                                                aGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCGCCAATTAACAGATGACGAAGCGGTTCTCCTTACCCCTCATCTGGAGCGACCAGCAGCTGCAAATgggcgg                                                        >  2:4036992/1‑140 (MQ=255)
                                                                                   tgctgcTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGCCGCTCCTCCCATTAACAGATGTCCAAGCGGGACTCCTTCCCCATCATATGCAGCGACCACCCGCGTCAAATGggggggcg                                                     >  2:1591113/1‑140 (MQ=255)
                                                                                       gctCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCGGCTCCTCCAATTAACACAGGACGAATCGGTCCTGCTTCCCCAACATCTGCACCTCCCACCACCGTCAAAAGGGCCGGCGTgcc                                                 >  1:2219466/1‑140 (MQ=255)
                                                                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                <  1:1498540/140‑1 (MQ=255)
                                                                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                <  1:1871498/140‑1 (MQ=255)
                                                                                        ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCCGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                <  1:3726621/140‑1 (MQ=255)
                                                                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTACTGGCTGGCCGCCAAATAACACAATACCAAGCGGTAATCCGAACCCAGCATCAGCAGCGACCAACAGCTTAAAAAGGGCGGGCGTCCCGGCGGt                                           >  1:3604756/1‑140 (MQ=255)
                                                                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACATATGACGAATCGGTACTACATCCCCATCATCTGCAGCTAACAGCAGCTTAAAAAGGGCGGGCGTCCCGTCGGt                                           >  2:547466/1‑140 (MQ=255)
                                                                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                           <  1:1941210/140‑1 (MQ=255)
                                                                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCGCCAAATTACACATGACGAAGCGGTACTCCTTCCCCCTCATCTGCAGCTACCAGCACCGTCAAATGGGGGGGCGTCCCGTCGGt                                           >  1:4089096/1‑140 (MQ=255)
                                                                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTGTTGGTAGTGCCTCTCCTCCAATTAACAGATTACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCCGCTTCAAAAGGGCGGGCGGCCCGTCGGt                                           >  2:1502574/1‑140 (MQ=255)
                                                                                             tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGTAGTGCCGGGCCTCCAAATAACAGATGTCGAAGCGGTACTCCTTCCCCCTCCTCTGCCGCGACCACCAGCTTCAAATGGGCGGGCGTCCcggcggg                                           >  1:3782641/1‑139 (MQ=255)
                                                                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGGCTCTCCTCCAAATAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGt                                      >  2:5058795/1‑140 (MQ=255)
                                                                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCGCCGCCAATTAACAGATTAAGAATCGGTACTCCTTCCCCAACCACTTCAGCTACCAGCAGCGTCAAATGGGCGGGCGGCCCGTCGGTTACGt                                      >  2:2568959/1‑140 (MQ=255)
                                                                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTCCTCCCAATAAAAAATGACGCAGCGGTACACCGTCCCCATCAAATGCAGCGACCCGCAGCGTGAAATGGGCGGGCGTCCCCGCGGTGACGt                                      >  1:3971630/1‑140 (MQ=255)
                                                                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGGAGTGCCGCTGCTCCCATTAACACATGACGAAGCGGTGCTCCGTACCCCTCAAATGCCGCTACCAGCAGCGTCAAATGGGGGGGGGGGCCGTCGGTTTCGt                                      >  1:852348/1‑140 (MQ=255)
                                                                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGCAGTGCCGCTCCTCCAAATAACAGATGAAGAAGCGGTACTCCTTGCCCCTCATCTGCAGCTACCAGCAGCGTCAAATGGGGGGGGGTCCCGTGGGTTACgg                                      >  2:3726628/1‑139 (MQ=255)
                                                                                                  gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTTCCGCTCCTCCAATTAACAGATTACGAAGCGGTACTCCTTCCCCCTCATCAGCAGCTACCACCAGCTTCAAAAGGGGGGGGGTCCCGGCGGGTACGt                                      >  2:3513454/1‑140 (MQ=255)
                                                                                                     aaaTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCaa                                   <  1:116630/140‑1 (MQ=255)
                                                                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTGCCCCTCAACTGCAGCAACCACCACCTTCAAATGGGGGGGCGTCCCCGCGGTTACCTCaaag                                 >  2:3381685/1‑138 (MQ=255)
                                                                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                 <  2:5199519/140‑1 (MQ=255)
                                                                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                 <  2:1028608/140‑1 (MQ=255)
                                                                                                       aTAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATg                                 <  2:4087652/140‑1 (MQ=255)
                                                                                                         aaGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                               <  2:3684457/140‑1 (MQ=255)
                                                                                                         aaGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                               <  1:1040305/140‑1 (MQ=255)
                                                                                                         aaGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                               <  2:2719789/140‑1 (MQ=255)
                                                                                                         aaGTGTATGAACTGGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                               <  2:4328923/140‑1 (MQ=255)
                                                                                                           gtgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCGTACCCATCATCTGCAGCTACCAGCAGCTTCAAAAGGGCGGGCGTCCCGTCGGTTACGTCAAAGCCAt                             >  2:14418/1‑140 (MQ=255)
                                                                                                            tgtATGAACTTGGTTACCGTCGTTTTTTGAACTTCCACTCCTCCAAATAACACATTAAGTAGCGGTAATACTTACCCATAATCTGCAGCTTCCAGCCGCGTAAAATTGGCGGGCGTGCCGGGGGGTAAGTCAATGCGTTg                            >  2:4787296/1‑140 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTTTTTTGAAATGCCTATCCTCCAATTAACAGATGAAGAAGCGGTACTCCATACCCATCATCTGCAGCAACCACCAGCGTCAAATGGGCGGGCGTCCCGTCGGTTAAGTCAAATCGATTCt                          >  2:2445374/1‑140 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTCACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                          <  1:2077153/140‑1 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                          <  2:3181631/140‑1 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                          <  1:2970702/140‑1 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                          <  1:4821723/140‑1 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                          <  2:4293292/140‑1 (MQ=255)
                                                                                                              tATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCACCCATTAACAGATTACGAAGCCGTACTACTTCCCCCTCAACAGCCGCTACCAGCACCTTAAAATGGGCGGGCGTCCCCGCGGGTAACTCAAAGCGATTCt                          >  2:635553/1‑140 (MQ=255)
                                                                                                                tGAACTTGGTTACCGTCGTTTTTTGAAGTTGCTCTGAGCCAATTAACAGATTACGAACCGGGACTACATACCCAACATCAGCAGCGACCAGAAGCTTAACATCGGGGGGCGGCCCGTCGGTTACGTCCAAGCGaatcttt                        >  2:1172865/1‑134 (MQ=255)
                                                                                                                tGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGt                        <  2:715882/140‑1 (MQ=255)
                                                                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTGTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTACCCATCATCTGCAGCTACCACCCGCTTCAAAAGGGCGGGCGGCCCGGCGGGTAAGTCAATGCCATTCTGTCGa                     >  2:3298320/1‑140 (MQ=255)
                                                                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTCACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                     <  1:3209771/140‑1 (MQ=255)
                                                                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                     <  2:4004969/140‑1 (MQ=255)
                                                                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                     <  1:685165/140‑1 (MQ=255)
                                                                                                                   aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCGACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                     <  1:4407723/140‑1 (MQ=255)
                                                                                                                    cTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAt                    <  1:3853729/140‑1 (MQ=255)
                                                                                                                      tGGTTACCGTCGTGTTTGGTAGTGCCTCGCCTCCAATTAACAGATTACGAAGCGGTACGCCTTCCCCAACATATGCAGCGACCAGCCGCTTCAAATGGGCGGGCGGCCCCGCGGGTACGTCAAAGCGAGGCTGTAGATAg                  >  2:1701151/1‑140 (MQ=255)
                                                                                                                         ttAGCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  2:4948773/140‑1 (MQ=255)
                                                                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  1:564374/140‑1 (MQ=255)
                                                                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  2:4856815/140‑1 (MQ=255)
                                                                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  2:4959218/140‑1 (MQ=255)
                                                                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  1:2826479/140‑1 (MQ=255)
                                                                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  1:2784182/140‑1 (MQ=255)
                                                                                                                         ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt               <  2:3536848/140‑1 (MQ=255)
                                                                                                                               tcgtTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCAt         <  1:4957379/140‑1 (MQ=255)
                                                                                                                                 gtTTTTGGAAGTGCCTCTCCACCAATTAAAAGATGACGAAGAGGTACTCCTTACCCAACATAAGCAGCTACCACCAGCTTCAAAAGGGCGGGCGTCCCGTCGGTTACCTCAATGCGATGCTGTCCATAATATTGGCaaaa       >  2:4754841/1‑140 (MQ=255)
                                                                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCATCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCATTGCGATGCTTTCGATAGTATTGGCATAAGGCCt  <  1:3345630/140‑1 (MQ=255)
                                                                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt  <  1:5214232/140‑1 (MQ=255)
                                                                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt  <  2:3045262/140‑1 (MQ=255)
                                                                                                                                      tGGAAGTGCCTCTCCTCCAATTAACAGATCACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGGATGGGCATAAGGCCt  <  1:3140933/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CAAGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCT  >  NZ_CP034943/1936458‑1936731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: